CN202610230U - Culture dish with separable culture area - Google Patents

Culture dish with separable culture area Download PDF

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Publication number
CN202610230U
CN202610230U CN 201220246639 CN201220246639U CN202610230U CN 202610230 U CN202610230 U CN 202610230U CN 201220246639 CN201220246639 CN 201220246639 CN 201220246639 U CN201220246639 U CN 201220246639U CN 202610230 U CN202610230 U CN 202610230U
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China
Prior art keywords
culture dish
culture
petridish
separable
separator
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Expired - Fee Related
Application number
CN 201220246639
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Chinese (zh)
Inventor
田颖
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Shaanxi University of Science and Technology
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Shaanxi University of Science and Technology
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Priority to CN 201220246639 priority Critical patent/CN202610230U/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a culture dish with a separable culture area. The culture dish with the separable culture area comprises a culture dish bottom and a culture dish cover. A separator is arranged in the culture dish bottom; the separator consists of one or more separation plates; and the diameter of the separator and the inner diameter of the culture dish bottom are equal. According to the culture dish with the separable culture area, through arranging the separator in the culture dish bottom, the culture dish bottom is evenly separated into two to five parts. After a bacterium suspension and a culture medium are poured into the culture dish, the sterile separator is put in the culture dish, and different separated areas naturally form on the condensed culture medium, and therefore, operations under different conditions are convenient, and even operating personnel with not enough skill can also well perform selection and division under different culture conditions. Not only can the operations with high technical requirements be simplified but also the large usage amount of the culture dish is saved, and therefore, effects that the operation is accurate, the labor is saved and the cost is saved are achieved.

Description

The regional separable petridish of a kind of cultivation
Technical field
The utility model belongs to the petridish technical field, relates to the regional separable petridish of a kind of cultivation.
Background technology
Now the used petridish specification in laboratory is a lot, is generally that diameter comprises 3.5,6,9, the petridish of 10cm, and its floorage is respectively 8,21,49,55cm 2, the amount of the substratum that can add is 3.0,5.0,10.0,10.0mL, obtainable cell concentration is respectively 2.0,5.2,12.2,13.7 * 10 6Individual.Wherein the application of diameter 9cm is more.
Above-mentioned petridish structure is identical, by at the bottom of one and a lid form.Generally make, as the equipment of culturing bacterium by glass or plastics.Yet screening when carrying out auxanography, chemical factor, when identifying mutant strain to experiment such as the influence of mikrobe and strain improvement, owing in the same space, operate, so a lot of problems appear in regular meeting, such as:
When 1, measuring auxanogram, operate careless slightlyly, the mycoderm phase sheet of interconnect that grows often occurs, consequently can not judge it is that which kind of growth factor is influencing the growth of bacterial strain actually.
2, inhibition zone that produces or the mutual interference of degraded transparent circle phase, accurate its diameter, the result have to revision test.
3, strain improvement time screening, need a large amount of petridish when identifying mutant strain, clean, sterilizing works is huge.
The utility model content
The technical problem that the utility model solves is to provide a kind of cultivation regional separable petridish, can petridish be separated into different zones, is convenient to cultivation and the screening of different condition mikrobe.
The utility model is to realize by the following technical programs:
The regional separable petridish of a kind of cultivation comprises at the bottom of the petridish and the petridish lid, is provided with separation scraper at the bottom of the petridish, and separation scraper is made up of one or more shims, the diameter of separation scraper and the internal diameter equal and opposite in direction at the bottom of the petridish.
Described separation scraper is made up of 1～5 shim, with evenly separating at the bottom of the petridish.
Compared with prior art, the utlity model has following beneficial technical effects:
The separable petridish in cultivation zone that the utility model provides through separation scraper is set, evenly is separated into 2～5 parts with it at the bottom of the petridish.After in petridish, pouring bacteria suspension and substratum into; To put into through the separation scraper of sterilization; Substratum forms different separated regions naturally after the condensation, thereby is convenient to the operation of different condition, even the skilled inadequately operator of technology also can well carry out the selection and the differentiation of different culture condition.
The separable petridish in cultivation zone that the utility model provides; The job simplification that hi-tech is required; And the usage quantity of having saved a large amount of petridish, thereby save washing and sterilizing work, significantly reduced experimenter's workload; Also save the purchase expenditure of petridish, thereby reached accurate, the laborsaving economic again effect of operation.
Description of drawings
Fig. 1-1～1-4 is the structural representation of separation scraper;
Fig. 2-1～2-4 is separation scraper dividing equally the different five equilibriums of petridish;
Fig. 3-1～3-4 is the vertical view divided equally of separation scraper with the different five equilibriums of petridish.
Wherein: 1 be petridish at the bottom of, 2 is separation scraper.
Embodiment
Below in conjunction with accompanying drawing the utility model is done to describe in further detail, said is explanation rather than qualification to the utility model.
Referring to Fig. 1-1～Fig. 3-4; The regional separable petridish of a kind of cultivation comprises at the bottom of the petridish and the petridish lid, is provided with separation scraper 2 in 1 at the bottom of the petridish; Separation scraper 2 is made up of one or more shims, 1 internal diameter equal and opposite in direction at the bottom of the diameter of separation scraper 2 and the petridish.
Concrete separation scraper can be made up of 1～5 shim, with evenly separating at the bottom of the petridish, shown in Fig. 2-1～Fig. 3-4, can it evenly be divided into 2,3,4,5 five equilibriums.
When using single lattice, can pour into different culture medium shown in Fig. 2-1 in the both sides of cutting apart, same bacterial classification is inoculated in both sides.For example the EMB substratum is poured in the left side; Common beef protein-high peptone substratum is poured on the right side, and both sides are inoculated intestinal bacteria respectively, can contrast the both sides colony characteristics.
When using many lattices, can pour into different culture medium shown in Fig. 2-2～2-4 in the both sides of cutting apart; Can in petridish, pour minimum medium (MM) and bacteria suspension earlier into; Wait to solidify back point in different separations and add various nutritional factor; With the nutrition pedigree of definite this bacterium, or the auxotrophy type of definite this bacterium.
The tyro uses this petridish separation scraper the first, can reduce the mortality of test greatly, even also can operate very accurately; The second, can reduce the usage quantity of petridish greatly, not only practiced thrift the workload that test dropped into but also avoided petridish washing, sterilization.
Therefore, the utility model not only can improve the precision of test, has solved puzzlement mikrobe worker's the huge traditional problem of workload again; Have bigger use value and marketable value.

Claims (2)

1. cultivate regional separable petridish for one kind, it is characterized in that, comprise at the bottom of the petridish and the petridish lid, be provided with separation scraper at the bottom of the petridish, separation scraper is made up of one or more shims, the diameter of separation scraper and the internal diameter equal and opposite in direction at the bottom of the petridish.
2. the regional separable petridish of cultivation as claimed in claim 1 is characterized in that described separation scraper is made up of 1～5 shim, with evenly separating at the bottom of the petridish.
CN 201220246639 2012-05-29 2012-05-29 Culture dish with separable culture area Expired - Fee Related CN202610230U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220246639 CN202610230U (en) 2012-05-29 2012-05-29 Culture dish with separable culture area

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Application Number Priority Date Filing Date Title
CN 201220246639 CN202610230U (en) 2012-05-29 2012-05-29 Culture dish with separable culture area

Publications (1)

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CN202610230U true CN202610230U (en) 2012-12-19

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773680A (en) * 2014-01-15 2014-05-07 珠海市银科医学工程有限公司 Superficial-fungi separated culture identification system
CN106085816A (en) * 2016-06-29 2016-11-09 安徽三彩工贸有限责任公司 A kind of Portable cell incubator
CN106119109A (en) * 2016-06-29 2016-11-16 安徽三彩工贸有限责任公司 A kind of pharmaceutical cell culture system
CN106338507A (en) * 2016-09-30 2017-01-18 中国科学院合肥物质科学研究院 Device for detecting biological effect of settling nano material-pollutant system and application
CN109679833A (en) * 2019-01-10 2019-04-26 航天神舟生物科技集团有限公司 Multifactor integration screening flat board and its tools and production method

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773680A (en) * 2014-01-15 2014-05-07 珠海市银科医学工程有限公司 Superficial-fungi separated culture identification system
CN106085816A (en) * 2016-06-29 2016-11-09 安徽三彩工贸有限责任公司 A kind of Portable cell incubator
CN106119109A (en) * 2016-06-29 2016-11-16 安徽三彩工贸有限责任公司 A kind of pharmaceutical cell culture system
CN106338507A (en) * 2016-09-30 2017-01-18 中国科学院合肥物质科学研究院 Device for detecting biological effect of settling nano material-pollutant system and application
CN106338507B (en) * 2016-09-30 2019-05-21 中国科学院合肥物质科学研究院 Sedimentation type nano material-pollution objects system biological effect detection device and application
CN109679833A (en) * 2019-01-10 2019-04-26 航天神舟生物科技集团有限公司 Multifactor integration screening flat board and its tools and production method
CN109679833B (en) * 2019-01-10 2021-10-26 航天神舟生物科技集团有限公司 Multi-factor integrated screening flat plate and manufacturing tool and manufacturing method thereof

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C17 Cessation of patent right
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Granted publication date: 20121219

Termination date: 20140529