CN209010535U - A kind of cell co-culture device - Google Patents
A kind of cell co-culture device Download PDFInfo
- Publication number
- CN209010535U CN209010535U CN201821651041.7U CN201821651041U CN209010535U CN 209010535 U CN209010535 U CN 209010535U CN 201821651041 U CN201821651041 U CN 201821651041U CN 209010535 U CN209010535 U CN 209010535U
- Authority
- CN
- China
- Prior art keywords
- culture
- cell
- fixed strip
- mounting groove
- frame
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model discloses a kind of cell co-culture devices of technical field of cell culture, the intracavity bottom of the culture box is placed with culture medium, co-cultivation frame is placed at the top of the culture medium, the top left side for co-culturing frame offers adipocyte culture chamber, the top right side for co-culturing frame offers myocyte's culture chamber, the top middle-end for co-culturing frame is welded with handle, the bottom for co-culturing frame is provided with miillpore filter, the bottom outside for co-culturing frame offers mounting groove, the bottom middle-end for co-culturing frame offers clamping slot, short fixed strip is mounted at left and right sides of the mounting groove, the two sides up and down of the mounting groove are mounted on long fixed strip, pass through the setting of this cell co-culture device, the fat cell of sheep and myocyte can be placed in a culture box and be co-cultured, culture Miillpore filter is replaced afterwards, can be utilized again, reduces manufacturing cost, waste rate is low, practical.
Description
Technical field
The utility model relates to technical field of cell culture, specially a kind of cell co-culture device.
Background technique
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology, no matter for
For entire biotechnology, or one of them biological cloning technology, cell culture is all an essential mistake
Journey, the extensive clone of cell culture inherently cell, cell culture technology can by a cell by mass propgation at
For simple many cells that are unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture itself
It is exactly the clone of cell, a large amount of cell or its metabolite is obtained by cell culture, because biological product is all from cell
It gets, it is possible to say that cell culture technology is most crucial, most basic technology in biotechnology.The fat cell and flesh of sheep
Cell is generally placed upon in two culture boxes during the cultivation process and cultivates, and cultivating box is disposable, manufacturing cost
Greatly, waste rate is high, for this purpose, we have proposed a kind of cell co-culture devices.
Utility model content
The purpose of this utility model is to provide a kind of cell co-culture devices, mentioned above in the background art to solve
The fat cell of sheep and myocyte are generally placed upon in two culture boxes during the cultivation process and cultivate, and cultivating box is one
Secondary property uses, and manufacturing cost is big, the high problem of waste rate.
To achieve the above object, the utility model provides the following technical solutions: a kind of cell co-culture device, including culture
The intracavity bottom of box, the culture box is placed with culture medium, and co-cultivation frame, the co-cultivation are placed at the top of the culture medium
The top left side of frame offers adipocyte culture chamber, and the top right side for co-culturing frame offers myocyte's culture chamber, institute
The top middle-end for stating co-cultivation frame is welded with handle, and the bottom for co-culturing frame is provided with miillpore filter, the co-cultivation frame
Bottom outside offer mounting groove, the bottom middle-end for co-culturing frame offers clamping slot, the left and right two of the mounting groove
Side is mounted on short fixed strip, and the two sides up and down of the mounting groove are mounted on long fixed strip, folder is equipped in the clamping slot
Tight plate, the miillpore filter are fixed on the bottom for co-culturing frame by short fixed strip, long fixed strip and clamping plate.
Preferably, the co-cultivation frame, short fixed strip, long fixed strip and clamping plate are that stainless steel is made.
Preferably, the aperture of the miillpore filter is 0.1-2 μm.
Preferably, the short fixed strip, long fixed strip are bolted in mounting groove, and the clamping plate passes through screw
It is fixed in clamping slot.
It, can be with compared with prior art, the utility model has the beneficial effects that by the setting of this cell co-culture device
The fat cell of sheep and myocyte are placed in a culture box and co-cultured, miillpore filter is carried out more after culture
It changes, can utilize again, reduce manufacturing cost, waste rate is low, practical.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is that the utility model co-cultures frame cross-sectional view；
Fig. 3 is that the utility model co-cultures frame bottom view.
In figure: 1 culture box, 2 culture mediums, 3 co-culture frame, 4 adipocyte culture chambers, 5 myocyte's culture chambers, 6 handles, 7
Miillpore filter, 8 mounting grooves, 9 clamping slots, 10 short fixed strips, 11 long fixed strips, 12 clamping plates.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-3 is please referred to, the utility model provides a kind of technical solution: a kind of cell co-culture device, including culture box
1, the intracavity bottom of the culture box 1 is placed with culture medium 2, and the top of the culture medium 2, which is placed with, co-cultures frame 3, described total
The top left side of culturing rack 3 offers adipocyte culture chamber 4, and the top right side for co-culturing frame 3 offers myocyte's training
Chamber 5 is supported, the top middle-end for co-culturing frame 3 is welded with handle 6, and the bottom for co-culturing frame 3 is provided with miillpore filter 7,
The bottom outside for co-culturing frame 3 offers mounting groove 8, and the bottom middle-end for co-culturing frame 3 offers clamping slot 9, institute
The left and right sides for stating mounting groove 8 is mounted on short fixed strip 10, and the two sides up and down of the mounting groove 8 are mounted on long fixed strip
11, clamping plate 12 is installed, the miillpore filter 7 passes through short fixed strip 10, long fixed strip 11 and clamping plate in the clamping slot 9
12 are fixed on the bottom for co-culturing frame 3.
Wherein, the co-cultivation frame 3, short fixed strip 10, long fixed strip 11 and clamping plate 12 are that stainless steel is made, intensity
Height is not easy to get rusty, and the aperture of the miillpore filter 7 is 0.1-2 μm, and the fat cell for preventing sheep and myocyte are from passing through micropore
The aperture of filter membrane 7, and the cell factor, nutritional ingredient, oxygen etc. in culture medium 2 can pass freely through, the short fixed strip 10,
Long fixed strip 11 is bolted in mounting groove 8, and the clamping plate 12 is fixed by screws in clamping slot 9, and installation is steady
It is fixed, convenient disassembly.
Working principle: miillpore filter 7 is mounted on culture 1 bottom surrounding of box by short fixed strip 10, long fixed strip 11
In mounting groove 8, the middle part of miillpore filter 7 is fixed in the clamping slot 9 for co-culturing 3 bottom middle-end of frame by clamping plate 12, is made
It is not easy cross-infection when being put into cell in adipocyte culture chamber 4 and myocyte's culture chamber 5, independently of each other, by the fat of sheep
Cell is put on the miillpore filter 7 of 4 bottom of adipocyte culture chamber, and myocyte is put into the micropore of 5 bottom of myocyte's culture chamber
On filter membrane 7, then box 1 is cultivated in micro- rolling, keeps cell distribution uniform, then the present apparatus is put into culturing room cultivated both can,
Miillpore filter 7 can facilitate the nutrient of culture medium 2 to pass through while cell being prevented to pass through, and the setting of handle 6 can facilitate taking-up to co-culture
Frame 3 will can replace miillpore filter 7 after the disassembly of short fixed strip 10, long fixed strip 11 and clamping plate 12.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (4)
1. a kind of cell co-culture device, including culture box (1), it is characterised in that: the intracavity bottom of culture box (1) is placed
Have culture medium (2), be placed at the top of the culture medium (2) and co-culture frame (3), the top left side for co-culturing frame (3) is opened
Equipped with adipocyte culture chamber (4), the top right side for co-culturing frame (3) is offered myocyte's culture chamber (5), the total training
The top middle-end for supporting frame (3) is welded with handle (6), and the bottom for co-culturing frame (3) is provided with miillpore filter (7), described total
The bottom outside of culturing rack (3) offers mounting groove (8), and the bottom middle-end for co-culturing frame (3) offers clamping slot (9),
It is mounted on short fixed strip (10) at left and right sides of the mounting groove (8), the two sides up and down of the mounting groove (8) are mounted on length
Fixed strip (11), the clamping slot (9) is interior to be equipped with clamping plate (12), and the miillpore filter (7) passes through short fixed strip (10), length
Fixed strip (11) and clamping plate (12) are fixed on the bottom for co-culturing frame (3).
2. a kind of cell co-culture device according to claim 1, it is characterised in that: the co-cultivation frame (3), short fixation
Item (10), long fixed strip (11) and clamping plate (12) are that stainless steel is made.
3. a kind of cell co-culture device according to claim 1, it is characterised in that: the aperture of the miillpore filter (7)
It is 0.1-2 μm.
4. a kind of cell co-culture device according to claim 1, it is characterised in that: the short fixed strip (10), length are solid
Determine item (11) to be bolted in mounting groove (8), the clamping plate (12) is fixed by screws in clamping slot (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821651041.7U CN209010535U (en) | 2018-10-11 | 2018-10-11 | A kind of cell co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821651041.7U CN209010535U (en) | 2018-10-11 | 2018-10-11 | A kind of cell co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209010535U true CN209010535U (en) | 2019-06-21 |
Family
ID=66838250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821651041.7U Expired - Fee Related CN209010535U (en) | 2018-10-11 | 2018-10-11 | A kind of cell co-culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209010535U (en) |
-
2018
- 2018-10-11 CN CN201821651041.7U patent/CN209010535U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2018014681A (en) | Single use bioreactor. | |
| CN203048951U (en) | Embedded multicell co-culture device | |
| US20120058560A1 (en) | Bioreactor System | |
| CN202610230U (en) | Culture dish with separable culture area | |
| CN209010535U (en) | A kind of cell co-culture device | |
| CN208562405U (en) | A kind of novel tissue culture plate | |
| CN208883905U (en) | The adjustable cell culture apparatus of medical experiment oxygen-supplying amount | |
| WO2023151260A1 (en) | Cell culture flask | |
| lan Freshney | Introduction to basic principles | |
| CN211057116U (en) | Carbon dioxide incubator with dual protection architecture | |
| CN207727073U (en) | A kind of microbial culture dish | |
| CN209602552U (en) | A kind of fixation device of the inoculating cell on common glass slide | |
| CN209836205U (en) | Animal cell incubator | |
| CN202415570U (en) | Integrated preparation rack for cell growing on glass slide | |
| CN207727085U (en) | A kind of ultraviolet shaking table | |
| CN203498381U (en) | Single cell culture vessel | |
| CN209368295U (en) | Cell culturing bracket | |
| CN107083355A (en) | A kind of feeder cells and preparation method and application | |
| CN206298577U (en) | It is suitable to culture dish and shifts special support | |
| CN103773683A (en) | Layered-type cell culture plate | |
| CN209030910U (en) | Breeding apparatus in a kind of insect larvae room convenient for operation | |
| CN208501003U (en) | A kind of biological cell cultivation sealing breeding apparatus | |
| CN204939497U (en) | A kind of cell culture bags | |
| CN105670914A (en) | Petri dish capable of continuously supplementing impact factors | |
| CN216039604U (en) | Test tube for biological cell culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190621 Termination date: 20201011 |