CN209602552U - A kind of fixation device of the inoculating cell on common glass slide - Google Patents
A kind of fixation device of the inoculating cell on common glass slide Download PDFInfo
- Publication number
- CN209602552U CN209602552U CN201821815523.1U CN201821815523U CN209602552U CN 209602552 U CN209602552 U CN 209602552U CN 201821815523 U CN201821815523 U CN 201821815523U CN 209602552 U CN209602552 U CN 209602552U
- Authority
- CN
- China
- Prior art keywords
- cell
- glass slide
- transverse slat
- pedestal
- fixation device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The fixation device of the utility model discloses a kind of on common glass slide inoculating cell comprising glass slide pedestal and the cell restriction device seperated with glass slide pedestal equipped with glass slide merging area；The glass slide two sides of base is equipped with the pedestal arm of force, and the pedestal arm of force is equipped with substrate latch fastener；The cell limits that device includes transverse slat and what is is flexibly connected set on transverse slat both ends and with transverse slat buckle with the matched transverse slat of substrate latch fastener, and the transverse slat is equipped with cell delimiter, and cell delimiter upper end is equipped with well, lower end is equipped with cell restriction hole.The step of saving production cell climbing sheet, and avoid that original common creep plate is easily broken, is not easy the problems such as clamping, is easy to be lost.
Description
Technical field
The utility model belongs to medical research product technical field, and in particular to one kind inoculating cell on common glass slide
Fixation device.
Background technique
Slide is exactly immersed in cell culture medium by cell climbing sheet, and cell is allowed to grow on slide, and then the cells are fixed,
For the histology of next step, immunohistochemistry, frozen section, cell smear, the experimental studies such as in situ hybridization.Existing market
On according to sold slide diameter be 8mm/14mm/20mm/25mm, be respectively suitable for 48 porocyte culture plates/24 hole cell culture
Plate/12 porocyte culture plates/6 porocyte culture plates.With preclinical medicine and life science especially gene and proteomics
The progress in equal fields, it is desirable that the technical indicator analyzed cell sample and observed is increasing, this is just needed often will be thin
Born of the same parents' creep plate, which is placed on glass slide, carries out microscope observation and analysis.
The sold slide of existing market is mostly designed according to the pore size of tissue culture plate, and diameter is often compared in culture plate
Diameter is slightly smaller in order to being placed in and take out.It cultivates in cell processes on creep plate it was found that creep plate centre cell number is more all
Edge substantially reduces, and cell distribution is uneven, and tracing it to its cause is to have thickness due to creep plate itself, and thin after inoculating cell on creep plate
Born of the same parents understand that natural sedimentation is adherent, so that cell is precipitated to the small gap between culture plate and creep plate more, creep plate periphery then occur
Cell is more, the few phenomenon of centrocyte, and the time of conventional cell creep plate production is long, and creep plate quality is generally relatively low.From culture
We need with special tweezers when taking out creep plate in plate, and creep plate is belittled transparent, is extremely easily broken and loses.In reality
It tests in research, one piece of creep plate, which can only often be inoculated with a kind of cell, to carry out one kind and research and analyse, required to grind if experimental cell type is more
It is more to study carefully index, generally requires a large amount of cell climbing sheet, workload is very big.In addition, when observing cell under the microscope and taking pictures, I
Also need for the one side on creep plate with cell to be placed on glass slide upward, then the covered on creep plate.Such creep plate
It is easily slided on glass slide, and light need to can just project on creep plate through wave carrier piece in high power microscopic observation cell,
Lowering clarity influences to take pictures, or even influences the shooting of laser co-focusing.Therefore, it is simple to seek a kind of production, convenient for taking pictures
The high-flux cell creep plate of observation is researcher urgent problem.
Utility model content
The utility model is intended to overcome easily broken conventional cell creep plate, easy to be lost, production complexity, cell distribution uneven, real
It tests that content is single, is not easy to the problems such as observing of taking pictures, a kind of fixation device of inoculating cell on common glass slide is provided.
In order to achieve the above object, technical solution provided by the utility model are as follows:
The fixation device of the inoculating cell on common glass slide includes the glass slide base equipped with glass slide merging area (2)
Seat and the cell seperated with glass slide pedestal limit device；The glass slide two sides of base is equipped with the pedestal arm of force (3), the pedestal
The arm of force (3) is equipped with substrate latch fastener (4)；The cell limits device and includes transverse slat (7) and be set to transverse slat (7) both ends and and transverse slat
(7) what is be flexibly connected buckles (6) with the matched transverse slat of substrate latch fastener (4), and the transverse slat (7) is equipped with cell delimiter (8), institute
It states cell delimiter (8) upper end and is equipped with cell restriction hole (10) equipped with well (9), lower end.
Preferably, the glass slide pedestal is equipped with cross bar (1).
Preferably, described be flexibly connected is hinged.
It is highly preferred that the transverse slat buckle (6) is hinged by movable axis (5) and transverse slat (7).
Preferably, the cell limits and is equipped with falope ring (11) on hole (10).
The utility model is described in further detail below:
Fixation device described in the utility model in common glass slide inoculating cell includes that glass slide pedestal and cell limit
Device.The glass slide pedestal, cell limit the transverse slat in device and buckle and be made of brown polypropylene (PP), and cell limits
Cell delimiter in device is made of colorless and transparent polypropylene (PP).Row power is erected there are two setting in glass slide pedestal both sides
Arm, arm of force apical lateral are equipped with buckle.Cell limits the buckle of device and glass slide substrate latch fastener is mutually matched with firm fixation.
The cell of cell delimiter limits and is equipped with falope ring on hole, will can strictly seal between cell delimiter and glass slide.Carefully
Born of the same parents' delimiter upper end is equipped with well, is not necessarily to seal on hole.
In specific implementation, it is 4cm that the pedestal, which erects row arm of force height, and wherein slide surface to base bottom height is
5mm, glass slide Platform Designing size is 3cmX8cm rectangle in pedestal, just matches various brand glass slide sizes.Base bottom
For sky, centre is equipped with 2 cross bars by bottom trisection, in order to support glass slide and observation cell.The cross of cell restriction device
Plate thickness is 3mm, and transverse slat is 35mm apart from glass slide upper level.Height in cell delimiter is 43mm, wherein transverse slat
Top is 5mm, is 3mm through transverse slat region, is 35mm below transverse slat, ignores the thickness of falope ring, carries to adapt to different-thickness
It can strictly be sealed when slide.The top well internal diameter of cell delimiter is 8mm, with adapt to the tip head of common 200ul into
Row changes liquid and intervention is added to handle, and lower section limits hole internal diameter as 15mm, to be inoculated with a certain number of cells as far as possible.Cell limits device
On buckle and the interlaced matching of substrate latch fastener, firm fixation, while ease of assembly and disassembly.Cell delimiter is on transverse slat
Distribution according to glass slide self character design (mark zone and observation area), limited in the region 6cm long of transverse slat side according to cell
Determining the size on device restriction hole aperture projection to transverse slat, trisection on row plate divides equally distribution by it.
The utility model compared with prior art the advantages of be:
The utility model is a kind of fixation device being directly seeded in cell on glass slide, saves production cell climbing sheet
The step of, it can be used to experimental study within cell one day after inoculation, and avoid that original common creep plate is easily broken, is not easy to press from both sides
The problems such as taking, be easy to be lost.Cell can be directly seeded on glass slide by the utility model, cell distribution on gained cell climbing sheet
It uniformly, is not in that original creep plate parietal cell is more, the problems such as centrocyte is few.The utility model cell delimiter is designed as justifying
Platform shape, high degree avoids the contaminated possibility of cell, while high degree saves cell.The utility model design has three
A cell limits hole, is independent of each other between each hole, can be inoculated with three kinds of different cells simultaneously, divide and do three kinds of different experiments researchs, together
When device itself a kind of restriction is provided, can greatly save experiment needed for reagent (fixer, antibody, dyestuff).The utility model
It is made of polypropylene (PP), is resistant to deep-frozen, high temperature high voltage resistant disinfection, acid-alkali-corrosive-resisting, it will not to experimental studies results
It has any impact, while the device can sterilize utilization repeatedly, without as using tissue culture plate when original production creep plate, this
Also experiment consumptive material has greatly been saved, experimental cost has been saved.The cell climbing sheet quality made of the utility model is higher, background
Completely, it is suitable for any microscope, and quality of taking pictures is high.
In short, the utility model provides a kind of fixation device being directly seeded in cell on glass slide, system is saved
The step of making cell climbing sheet, and avoid that original common creep plate is easily broken, is not easy the problems such as clamping, is easy to be lost.This is practical new
Cell can be directly seeded on glass slide by type, and cell distribution is uniform on gained cell climbing sheet, be not in original creep plate week
The problems such as marginal cell is more, and centrocyte is few.The utility model cell delimiter is designed as truncated cone-shaped, and high degree avoids cell
Contaminated possibility, while high degree saves cell.There are three cells to limit hole for the utility model design, between each hole mutually
It does not influence, three kinds of different cells can be inoculated with simultaneously, divide and do three kinds of different experiments researchs, while device itself provides a kind of restriction,
Reagent (fixer, antibody, dyestuff) needed for experiment can be greatlyd save.The utility model is made of polypropylene (PP), is resistant to depth
Cryogenic freezing, high temperature high voltage resistant disinfection, acid-alkali-corrosive-resisting, will not have any impact to experimental studies results, while the device
Utilization can be sterilized repeatedly, it is not necessary that as using tissue culture plate when original production creep plate, this has also greatly saved experiment consumptive material,
Experimental cost is saved.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the fixed device of the utility model；
Fig. 2 is the top view that cell limits device in the fixed device of the utility model；
Fig. 3 is the top view of pedestal in the fixed device of the utility model.
In figure: 1, cross bar, 2, glass slide merging area, 3, the pedestal arm of force, 4, substrate latch fastener, 5, movable axis, 6, transverse slat buckle,
7, transverse slat, 8, cell delimiter, 9, well, 10, cell restriction hole, 11, falope ring.
Specific embodiment
Referring to Fig. 1 to Fig. 3, the fixation device of the inoculating cell on common glass slide includes being equipped with glass slide to be placed in area
2 glass slide pedestal and the cell seperated with glass slide pedestal limit device；The glass slide two sides of base is equipped with the pedestal arm of force
3, the pedestal arm of force 3 is equipped with substrate latch fastener 4；The cell limit device include transverse slat 7 and be set to 7 both ends of transverse slat and with cross
Plate 7 by movable axis 5 it is hinged with the matched transverse slat of substrate latch fastener 4 buckle 6, the transverse slat 7 is equipped with cell delimiter 8, institute
It states 8 upper end of cell delimiter and is equipped with cell restriction hole 10 equipped with well 9, lower end.
Wherein, the glass slide pedestal is equipped with cross bar 1.The cell limits and is equipped with falope ring 11 on hole 10.
The application method of the fixation device of the inoculating cell on common glass slide are as follows: using it is preceding by glass slide pedestal and
Cell limits device and is placed on sterilization in high-pressure steam sterilizing pan, and it is small that falope ring is placed in soaking disinfection 2 in 75% alcohol
When, by 75% alcohol wipe surface of common glass slide.Then the falope ring after disinfection is mounted on the cell that cell limits device
It limits on hole, then all objects are placed in aseptic operating platform and use ultraviolet-ray sterilizing 30 minutes.Then the load glass that will be disinfected
Piece is placed in pedestal, and cell restriction device is mounted on glass slide, substrate latch fastener and transverse slat are buckled clamping, with that can be with
By well into the utility model inoculating cell.After cell to be seeded is adherent, base can be passed through under inverted microscope
The lower square hole of seat observes cell growth condition on glass slide and takes pictures.Into cell delimiter plus it can be intervened by well simultaneously
Or replacement culture solution, subsequent cell, which fixes, dyes, hatch the experimentations such as primary antibody and secondary antibody is not necessarily to dismantle cell to limit and fills
It sets, multiple cells limited in hole and experiment content are independent of each other, and cell limit is removed when last cell is transparent or mounting
Determine device, then gives mounting and observe to take pictures.Using the utility model make cell climbing sheet can save to greatest extent cell,
Time, consumptive material and reagent, made cell climbing sheet quality is guaranteed and conveniently takes pictures.
Claims (5)
1. a kind of fixation device of the inoculating cell on common glass slide, which is characterized in that the fixed device includes being equipped with to carry
Slide is placed in the glass slide pedestal of area (2) and the cell seperated with glass slide pedestal limits device；The glass slide two sides of base
Equipped with the pedestal arm of force (3), the pedestal arm of force (3) is equipped with substrate latch fastener (4)；It includes transverse slat (7) that the cell, which limits device,
(6) are buckled with the matched transverse slat of substrate latch fastener (4) with what is be flexibly connected set on transverse slat (7) both ends and with transverse slat (7), the transverse slat
(7) cell delimiter (8) are equipped with, cell delimiter (8) upper end is equipped with well (9), lower end is equipped with cell and limits hole
(10).
2. as described in claim 1 on common glass slide inoculating cell fixation device, which is characterized in that the glass slide
Pedestal is equipped with cross bar (1).
3. as described in claim 1 on common glass slide inoculating cell fixation device, which is characterized in that it is described activity even
It is connected in hinged.
4. as claimed in claim 3 on common glass slide inoculating cell fixation device, which is characterized in that the transverse slat card
It is hinged by movable axis (5) and transverse slat (7) to detain (6).
5. as described in claim 1 on common glass slide inoculating cell fixation device, which is characterized in that cell limit
Determine to be equipped with falope ring (11) on hole (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821815523.1U CN209602552U (en) | 2018-11-06 | 2018-11-06 | A kind of fixation device of the inoculating cell on common glass slide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821815523.1U CN209602552U (en) | 2018-11-06 | 2018-11-06 | A kind of fixation device of the inoculating cell on common glass slide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209602552U true CN209602552U (en) | 2019-11-08 |
Family
ID=68394036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821815523.1U Expired - Fee Related CN209602552U (en) | 2018-11-06 | 2018-11-06 | A kind of fixation device of the inoculating cell on common glass slide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209602552U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652311A (en) * | 2018-11-06 | 2019-04-19 | 中南大学湘雅二医院 | A kind of restriction device that cell is directly seeded in glass slide |
-
2018
- 2018-11-06 CN CN201821815523.1U patent/CN209602552U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652311A (en) * | 2018-11-06 | 2019-04-19 | 中南大学湘雅二医院 | A kind of restriction device that cell is directly seeded in glass slide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5674953B2 (en) | Cell culture insert | |
| Katzenell et al. | Isolation, purification, and culture of primary murine sensory neurons | |
| Ausubel et al. | GMP scale-up and banking of pluripotent stem cells for cellular therapy applications | |
| CN105861428B (en) | A kind of induced fibroblast transdifferentiation is the induced medium and its application of cardiac muscle cell | |
| US20120058560A1 (en) | Bioreactor System | |
| CN209602552U (en) | A kind of fixation device of the inoculating cell on common glass slide | |
| Reed | Culture systems: embryo density | |
| Lopez-Ramirez et al. | Isolation and culture of adult zebrafish brain-derived neurospheres | |
| CN204644371U (en) | A kind of integrated form many cells noncontact co-culture device | |
| CN104130943B (en) | Neuron and the orderly co-culture device of neurogliocyte, preparation method and neuron and the orderly co-culture method of neurogliocyte | |
| JP6401818B2 (en) | Method for producing activated stem cells | |
| CN109652311A (en) | A kind of restriction device that cell is directly seeded in glass slide | |
| Ramirez et al. | Protocol for controlled cortical impact in human cerebral organoids to model traumatic brain injury | |
| Helfer et al. | Frame-hydrogel methodology for engineering highly functional cardiac tissue constructs | |
| CN204434602U (en) | A kind of planktonic algae cell microwell array culture apparatus | |
| CN216998452U (en) | Cell slide culture apparatus | |
| Hammitt et al. | Prediction of nuclear maturity from cumulus-coronal morphology: influence of embryologist experience | |
| Macleod et al. | Essential techniques of cancer cell culture | |
| CN103305418A (en) | Stem cell isolated culturing device | |
| Chu et al. | Custom-built tools for the study of deer antler biology | |
| Rienzi et al. | Oocyte denuding | |
| CN203668416U (en) | Cover glass fixing clamp for cell culture | |
| RU218544U1 (en) | micro surgical chamber | |
| JP2005218368A (en) | Apparatus for culturing cell | |
| CN112961829B (en) | Embryonic spinal cord in-vitro culture system, embryonic spinal cord in-vitro hypoxia injury model establishing method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191108 Termination date: 20211106 |