CN203668416U - Cover glass fixing clamp for cell culture - Google Patents

Abstract

Acover glass fixing clamp for cell culture can be used for fixing a piece ofcover glass conveniently, running through the whole process of cover glassculture of whole cells, reducing the damage to the cells on the cover glass and improving the operation efficiency. The fixing clamp is in an n shape consisting of three vertical glass panels, the two opposite glass panels are parallel and provided with horizontal grooves at the same heightrespectively, the two glass panels are provided with horizontally-extending rectangular panels with holes respectively, the third glass panel is perpendicular to the other two glass panels, and a square hole is formed in the middle of the third glass panel. The cover glass can be inserted into the grooves horizontally to be disinfected together with the fixing clamp before being used for cell culture, then both the fixing clamp and the cover glass are placed in a culture dish, primary cellculture or passage cellculture is performed on the cover glass, and various operations such as culture solution replacement, culture dish replacement, inverted microscope observation, cell staining and the like can be realized through clamping of the rectangular panels with the holes of the fixing clamp instead of direct clamping operation performed on the cover glass through tweezers, so that damage caused by instruments to the cells on the cover glass is avoided, and the operation efficiency is improved. If the processes are finished completely, the cover glass can be pushed out lightlythrough the square hole in the center of the transverse glass panel.

Description

A kind of cover glass fixing catch for cell cultures

Technical field

The utility model belongs to experiment apparatus technical field, designs a kind of glass and makes the cover glass fixing catch for cell cultures of being convenient to clamping operation.

Background technology

Many experiments in life science field, particularly relate in the experiment of cell cultures, usually need in culturing process, cell be seeded on cover glass, and in the time that cell growth meets experiment condition, cover glass must be taken out from culture dish to operations such as doing further form dyeing mensuration, patch clamp.At present, the experimental implementation that cover glass is taken out from culture dish, typically uses pincet cover glass is picked up.But can cause so many problems: the first, due to the existence of nutrient solution, washings, cover glass contacts conventionally tightr with culture dish, and the tip of pincet is tapering not, is not easy smoothly cover glass to be provoked; The second, even if provoke cover glass, if clamping not in time, cover glass very easily falls back in culture dish, is usually stacked in other above cover glass, or upset occurs cover glass and cause cell attaching to face down, and cannot carry out subsequent experimental; The 3rd, cover glass positive and negative (having cell to attach face and acellular attaching face) naked eyes are difficult for identification, in experimentation, cause reverse side not found in time upward and because of stirring, and all follow-up experimental procedures are all in vain, waste of manpower, material resources; The 4th, cover glass is clamped by pincet, because local pressure is inhomogeneous, easily breaks; The 5th, experimentation often needs repeatedly removable cover slide, and the clamping repeatedly of pincet will inevitably cause the cell injury in multiple regions, affects experimental result.

In recent years, researcher had designed some apparatuses, such as cell cultures pathological staining box (plate), was applicable to a large amount of immunocyte dyeing, had solved above-mentioned subproblem, if but sample is less, will cause waste; And in the experiments such as many cell function mensuration, must be using cover glass as cell carrier, so quick, the safety holding of cover glass just become a major issue urgently to be resolved hurrily.

Once designed before us a kind of cover glass fetching device ( 200820029858.0), in application in practice, solve to a great extent Similar Problems, but still had following defect: 1., in the time that needs are inverted cover glass, this apparatus cannot have been implemented effectively, the operation of harmless cell; 2. only rely on a press strip fixed cap slide, fixed effect is still imprecise; 3. this apparatus can only could use in the time of the operations such as dyeing, observation, and cell cultivation process cannot use.Based on this, we improve in the practice that uses cover glass fetching device, be subject to fund project to subsidize (National Nature fund 30900585, National Nature fund 81100056, Shanxi Province basic research project 2011021038-2), designed cover glass fixing catch.

Summary of the invention

The purpose of this utility model is to provide a kind of glass and makes the cover glass fixing catch for cell cultures of being convenient to clamping operation, to overcome the deficiencies in the prior art.

The technical scheme that the utility model adopts is: cover glass fixing catch forms " П " shape by three vertical sheet glass, parallel and the equal-height position of two relative sheet glass respectively has a horizontal groove, two sheet glass rectangular slabs with holes that each own level is stretched out respectively, the 3rd surface glass plate is vertical with other two sheet glass, and there is a square opening centre.Before use cover glass carries out cell cultures, cover glass level can be inserted to groove, disinfect together, then fixing catch and cover glass are together placed in culture dish, on cover glass, carry out primary or passage cell cultivation, change the various operations such as nutrient solution, replacing culture dish, inverted microscope observation and cell dyeing, all can realize by adding the rectangular slab with holes of holding in fixing catch, and do not need, with tweezers, cover glass is carried out to directly clamping operation, avoid the damage of apparatus to the cell on cover glass, improve operation efficiency.If above-mentioned flow process all finishes, can cover glass be released gently by the square opening of horizontal sheet glass central authorities, carry out the operations such as gummy mounting or lysing cell.In experimentation, can there is not the situation that cover glass pros and cons is put upside down yet.The specification of cover glass has multiple, and the most frequently used about 3 ~ 5 kinds, the width of fixing catch can design several different sizes and meet the width of cover glass.But the thickness of groove is generally not more than 0.3mm, the whole height of fixing catch is not more than 6mm.

Compared with the cover glass fetching device of our design before, cover glass fixing catch has following innovation and advantage: 1. that cover glass is fixing up and down, when needs, cell to be inverted while cultivating, and overturn cover slide fixing catch just can easily realize; 2. parallel groove design guarantees that cover glass can not come off; 3. the height of cover glass fixing catch is less than the height of general culture dish, can easily be placed in culture dish together with cover glass, in the whole process of cell cultures, dyeing, the experimental technique such as fixing, can directly not contact cover glass, easy and simple to handle, reduce the damage to cell.

The beneficial effects of the utility model are, fixed cap slide easily, and be through to whole cell cover glass cultivate whole process, all can realize by adding the rectangular slab with holes of holding in fixing catch, and do not need, with tweezers, cover glass is carried out to directly clamping operation, avoid the damage of apparatus to the cell on cover glass, improve operation efficiency.

Accompanying drawing explanation

Fig. 1 is cover glass fixing catch overall schematic: 1 groove, the rectangular slab with holes that 2 levels are stretched out, 3 square openings

Fig. 2 is that cover glass inserts the schematic diagram after fixing catch.

Embodiment

For clearly demonstrating the technical characterstic of this programme, below by an embodiment, and by reference to the accompanying drawings, this programme is set forth.

As shown in Figure 1, cover glass fixing catch forms " П " shape by three vertical sheet glass, parallel and its equal-height position of two relative sheet glass respectively has a horizontal connected in star, two sheet glass rectangular slabs with holes that each own level is stretched out respectively, the 3rd surface glass plate is vertical with other two sheet glass, and there is a square opening centre.As shown in Figure 2, cover glass is passed through to the horizontal groove of both sides sheet glass equal altitudes, shift onto in fixing catch, the whole or most of of cover glass are just fixed in cover glass fixing catch relatively, add and hold the rectangular slab with holes that in fixing catch, level is stretched out, cover glass can be put into or taken out of culture dish together with fixing catch.

Claims (5)

1. the cover glass fixing catch for cell cultures, it is made up of three vertical contour sheet glass, it is characterized in that: two relative sheet glass are parallel, the rectangular slab with holes that respectively has a horizontal groove and level to stretch out, the 3rd surface glass plate is vertical with other two sheet glass, and there is a hole centre.

2. a kind of cover glass fixing catch for cell cultures according to claim 1, is characterized in that, wherein said hole is square or circular.

3. a kind of cover glass fixing catch for cell cultures according to claim 1, is characterized in that, parallel two relative sheet glass, respectively have a horizontal groove, are positioned at opposite face and contour.

4. a kind of cover glass fixing catch for cell cultures according to claim 1, is characterized in that, parallel two relative sheet glass, and the rectangular slab with holes that respectively has a level to stretch out, lays respectively at sheet glass top and bottom.

5. a kind of cover glass fixing catch for cell cultures according to claim 1, is characterized in that, the 3rd surface glass plate and all vertical being " П " of parallel two relative sheet glass, and there is a square opening centre.

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