CN202175681U - Cell climbing slice culture plate - Google Patents

Cell climbing slice culture plate Download PDF

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Publication number
CN202175681U
CN202175681U CN 201120308698 CN201120308698U CN202175681U CN 202175681 U CN202175681 U CN 202175681U CN 201120308698 CN201120308698 CN 201120308698 CN 201120308698 U CN201120308698 U CN 201120308698U CN 202175681 U CN202175681 U CN 202175681U
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China
Prior art keywords
culture
cell
culture plate
groove
cell climbing
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Expired - Fee Related
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CN 201120308698
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Chinese (zh)
Inventor
李大伟
徐忠华
刘海南
闫磊
任巨超
刘吉凯
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Shandong University
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Shandong University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/12Well or multiwell plates

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

一种细胞爬片培养板,培养板上设有若干个培养孔,每个培养孔大小和形状相同,在培养孔的底部有一条经过培养孔底部中心的凹槽。所述的凹槽的截面形状为圆弧形或矩形。细胞爬片培养板主要由常规的培养板组成,在培养孔的底部设置一条经过中心的凹槽,作为培养板与细胞爬片之间的间隙,科研及医务人员在实验过程中可以经此凹槽快速准确的取出细胞爬片,进行下一步的实验操作。本实用新型设备加工简单、易于制造、成本低、结构合理、操作方便、省时省力,节约取出细胞爬片的时间,降低损坏细胞爬片的机率,进而提高了科研及医务人员的工作效率,节省实验试剂,保证实验质量。

Figure 201120308698

A cell slide culture plate is provided with a number of culture holes, each of which has the same size and shape, and a groove passing through the center of the bottom of the culture hole at the bottom of the culture hole. The cross-sectional shape of the groove is arc or rectangle. The cell slide culture plate is mainly composed of conventional culture plates. A groove passing through the center is set at the bottom of the culture well as a gap between the culture plate and the cell slide. Scientific research and medical personnel can pass through this groove during the experiment. The cell slides are quickly and accurately removed from the tank, and the next step of the experimental operation is carried out. The utility model equipment is simple in processing, easy to manufacture, low in cost, reasonable in structure, convenient in operation, saves time and labor, saves the time for taking out cell slides, reduces the probability of damaged cell slides, and improves the work efficiency of scientific research and medical personnel. Save experimental reagents and ensure the quality of the experiment.

Figure 201120308698

Description

The cell climbing sheet culture plate
Technical field
The utility model relates to a kind of improved cell climbing sheet culture plate, belongs to the cell culture apparatus technical field.Being mainly used in cell climbing sheet cultivates.This cell climbing sheet culture plate is characterised in that: be made up of culture plate, culture hole and loam cake, culture plate has several culture hole, and each culture hole size, shape are identical, periphery have sidewall center on the hole around, and a groove through the center is arranged at the bottom of culture hole.The utility model is rational in infrastructure, be easy to make, processing units is simple, cost is low; Easy to operate, time saving and energy saving; Can help the operator easily and accurately, gripping helps increasing work efficiency and shortens experimental period, the damage carrier cover slide (cell climbing sheet) in avoiding testing wherein as the deckglass of cell culture vector apace; Practice thrift experimental cost, have application value and vast market prospect.
Background technology
Cell in vitro is cultivated the influence of the complicated disturbance factor of having got rid of in vivo tests; Help exploring the rule of cellular activity; Significant in healthy and life science, often need carry out common dyeing observation of cell form or specific stain analysis of cells molecular distribution.At present, mostly the Tissue Culture Plate that culturing cell uses in biological experiment is circular hole, flat like 2,4,6,24,96 holes, also is usually used in cell climbing sheet and makes.Conventional method of making the attached cell creep plate is as the carrier of making cell climbing sheet with deckglass; In Tissue Culture Plate, make cell climbing sheet then; Yet closely contact between deckglass and the culture plate, cause and take out the cell climbing sheet difficulty, the running time prolongs; Cause in dyeing and microscopy process, making the not good even apoptosis of cell growth state, and shortcomings such as fragile cell climbing sheet, conventional efficient and success ratio reduction are arranged.
Yet; Existing several kinds of cell climbing sheet culture plates invention is not widely used in clinical and the scientific effort as yet; Its possible cause is: processing units requires height, tooling cost is high, manufacturing is complicated; Complex operation, waste time and energy, cost an arm and a leg, can not help the operator to increase work efficiency with experiment success rate effectively etc.
Summary of the invention
To the deficiency of prior art, the utility model provides a kind of cell climbing sheet culture plate.
A kind of cell climbing sheet culture plate, culture plate is provided with several culture hole, and each culture hole size is identical with shape, and a groove through culture hole bottom centre is arranged in the bottom of culture hole.
The cross-sectional shape of described groove is circular arc or rectangle.
The cell climbing sheet culture plate mainly is made up of the culture plate of routine; Groove through the center is set in the bottom of culture hole; As the gap between culture plate and the cell climbing sheet; Scientific research and medical worker can take out cell climbing sheet through this groove fast and accurately in experimentation, carry out next step experimental implementation.
The utility model apparatus processing is simple, be easy to make, cost is low, rational in infrastructure, easy to operate, time saving and energy saving; Practice thrift the time of taking out cell climbing sheet, reduce the probability of damaging cell climbing sheet, and then improved scientific research and medical worker's working efficiency; Save experiment reagent, guarantee the experiment quality.
Description of drawings
Fig. 1 is the vertical view of the utility model.
Fig. 2 is the vertical view of culture hole in the utility model.
Fig. 3 is the structural profile synoptic diagram of the utility model.
Wherein: 1, Tissue Culture Plate, 2, culture hole, 3, the bottom of culture hole, 4, through the groove of culture hole bottom centre.
Embodiment:
Below in conjunction with accompanying drawing and embodiment the utility model is described further.
Embodiment 1:
A kind of cell climbing sheet culture plate, to shown in Figure 3, culture plate (1) is provided with 6 culture hole (2) like Fig. 1, and each culture hole (2) size is identical with shape, and a groove (4) through (3) center, culture hole bottom is arranged in the bottom (3) of culture hole (2).
The cross-sectional shape of described groove (4) is a circular arc.
On the bottom (3) of cell climbing sheet culture plate (1) culture hole (2), a semi-cylindrical recesses (4) is set.
Described groove (4) is provided with the bottom (3) of cell climbing sheet culture plate culture hole, and through the center of culture hole, and direction is an any direction.
At the groove (4) of cell climbing sheet culture plate culture hole bottom (3), as the gap between cell climbing sheet and culture hole (2) bottom surface, operator are in experimentation;, conveniently take out cell climbing sheet quickly and accurately and carry out next step experimental implementation as reference with groove (4), practice thrift the time of gripping cell climbing sheet in the experimentation; Improve operation efficiency; And can reduce the probability that the histocyte sample damages, save experiment reagent, guarantee the experiment quality.
Embodiment 2:
A kind of cell climbing sheet culture plate, structure is identical with embodiment 1, and difference is that the cross-sectional shape of described groove (4) is a rectangle.

Claims (2)

1. a cell climbing sheet culture plate is characterized in that, culture plate is provided with several culture hole, and each culture hole size is identical with shape, and a groove through culture hole bottom centre is arranged in the bottom of culture hole.
2. cell climbing sheet culture plate as claimed in claim 1 is characterized in that, the cross-sectional shape of described groove is circular arc or rectangle.
CN 201120308698 2011-08-23 2011-08-23 Cell climbing slice culture plate Expired - Fee Related CN202175681U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120308698 CN202175681U (en) 2011-08-23 2011-08-23 Cell climbing slice culture plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201120308698 CN202175681U (en) 2011-08-23 2011-08-23 Cell climbing slice culture plate

Publications (1)

Publication Number Publication Date
CN202175681U true CN202175681U (en) 2012-03-28

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018107437A1 (en) * 2016-12-15 2018-06-21 广州洁特生物过滤股份有限公司 Coverslip for cell culture

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018107437A1 (en) * 2016-12-15 2018-06-21 广州洁特生物过滤股份有限公司 Coverslip for cell culture
US11221475B2 (en) 2016-12-15 2022-01-11 Guangzhou Jet Bio-Filtration Co., Ltd. Coverslip for cell culture

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120328

Termination date: 20140823

EXPY Termination of patent right or utility model