CN209428540U - A tissue culture dish assembly - Google Patents

Abstract

A kind of tissue culture dishes component described in the utility model, including ware lid and ware bottom, and be provided with matched with ware bottom inner cavity suit overlay comb, the upper surface center for overlaying comb is provided with card slot, deck has clump weight in card slot, can compressed tissue block so that it is not easily disconnected from ware bottom bottom, branch fastener is vertically distributed in the lower surface for overlaying comb, each branch fastener presses against the tissue block upper end of ware bottom inner cavity respectively, overlays and air hole is distributed on comb.The tissue culture dishes component can both guarantee the aerobic environment that tissue cultures need, and tissue block can be effectively ensured do not float in culture solution again in conjunction with the use of clump weight, can adherent growth.

Description

A tissue culture dish assembly

technical field

The utility model belongs to the technical field of tissue culture experiment equipment, in particular to a tissue culture dish assembly.

Background technique

As a kind of experimental vessel for microorganism or tissue culture, Petri dish is widely used in biological experiments. Due to the different growth conditions and growth methods of different biological tissues, the culture methods are also different. The traditional petri dish structure is composed of a disc-shaped bottom and a cover. It needs to be turned upside down and placed flat in an incubator, which is difficult to meet the cultivation needs of all samples to be cultured. Especially for the cultivation of some aerobic tissues, an aerobic environment is required during the cultivation process, which requires the culture dish to be capable of gas exchange, and it is necessary to ensure the adherent growth of the tissue at all times; When liquid culture medium is poured into the dish or during the operation, the tissue pieces will float in the culture dish. Once the tissue pieces leave the bottom of the dish, the culture will fail and the tissue culture needs to be repeated; Buckling and fastening with the bottom of the dish is also not conducive to gas exchange during tissue culture, which affects the culture effect of the tissue block.

Contents of the invention

Aiming at the defects and problems of traditional petri dishes that affect tissue aerobic respiration and are difficult to adhere to the wall, the utility model provides a petri dish component that is convenient for tissue to adhere to the wall.

The solution adopted by the utility model to solve the technical problem is: a tissue culture dish assembly, including a dish cover and a dish bottom, and a pressing grate matching with the inner cavity of the dish bottom, the lower surface of the pressing grate is vertical There are supporting heads distributed in the direction, and each supporting head is respectively pressed against the upper end of the tissue block in the bottom of the dish, and air holes are distributed on the pressing grate.

In the aforementioned tissue culture dish assembly, a slot is provided at the center of the upper side of the pressing grate and a counterweight is mounted on the seat.

In the above-mentioned tissue culture dish assembly, pull rings that can be pulled up are arranged symmetrically on both sides of the slot, and the pull rings are integrally formed with the pressing grate.

Beneficial effects: in the tissue culture dish assembly described in the utility model, air holes are evenly distributed on the pressing grate, and the air holes can effectively ensure the gas exchange of tissue culture; the upper surface of the pressing grate is provided with a slot, Matching weights are placed in the groove; support heads supporting the grate are distributed on the lower surface of the pressing grate. It effectively solves the problem that the tissue block is not easy to adhere to the wall when it is floating in the culture medium, and ensures the normal growth of the tissue block.

Description of drawings

Fig. 1 is a schematic diagram of the assembly structure of the utility model;

Fig. 2 is the structural representation when the utility model presses and pastes the grate and the bottom of the dish is buckled;

Fig. 3 is a structural schematic diagram one of the upper surface of the pressing grate;

Fig. 4 is a structural schematic diagram one of the lower surface of the pressing grate;

Fig. 5 is the schematic diagram of the three-dimensional structure of the pressing grate;

Fig. 6 is the second structural schematic diagram of the lower surface of the pressing grate.

Among the figure: 1 is the lid of the dish, 2 is the bottom of the dish, 3 is the pressing grate, 4 is the vent hole, 5 is the support head, 6 is the tissue block, 7 is the slot, 8 is the counterweight, and 9 is the pull ring.

Detailed ways:

Below in conjunction with accompanying drawing and embodiment the utility model is further described.

Embodiment 1: A kind of tissue culture dish assembly of this embodiment, as shown in Figure 1 and Figure 2, comprises by dish lid 1 and dish bottom 2, and dish bottom inner cavity is matched with the pressing grate 3, and pressing grate 3 It is matched and fastened with the bottom of the dish. Petri dishes of different sizes are equipped with matching pressing grates; two air holes 4 are symmetrically distributed along the center line on the pressing grate 3. The setting of the air holes 4 can ensure that the culture dish can be connected with the Gas exchange is carried out externally, as shown in Figure 3, the center position of the upper surface of the pressing grate 3 is provided with a draw-in groove 7, and the deck in the draw-in groove 7 has a counterweight 8; The surface is symmetrically provided with supporting heads 5 supporting the pressing grate along the midline, and each supporting head 5 presses against the upper end of the tissue block 6 in the bottom of the dish respectively.

Since the biological experiment needs to be carried out in a sterile environment, the tissue culture dish assembly of the utility model can be guaranteed to be carried out in a sterile environment during the actual use. The experimenter cuts the tissue into uniform sizes on the sterile operating table For the tissue block 6, use the sterilized tweezers with the left hand to pick up the pressing grate 3, and use the tweezers for the right hand to put the tissue block 6 into the center of the inner cavity at the bottom of the dish, cover the pressing grate 3, and press the tissue block 6 on top of each supporting head. Then put the counterweight 8 in the draw-in groove 7 to ensure that the tissue block is close to the bottom of the dish and will not move easily, and then pour the sterilized and cooled liquid culture solution from the vent hole 4 into the inner cavity of the bottom of the dish, and then Cover the dish lid 1, place the culture dish body in an incubator suitable for the growth of tissue blocks for cultivation, and ensure that the tissue blocks are always close to the bottom of the dish during the whole process.

Embodiment 2: A tissue culture dish assembly of this embodiment, the similarities with Embodiment 1 will not be repeated, the difference is that, as shown in Figure 5, both sides of the card slot 7 are symmetrically provided with pressing and pasting The pull ring 9 integrally formed by the grate 3 can ensure that the lid 1 can be matched and fastened with the bottom 2 of the dish; the setting of the pull ring 9 is convenient for lifting the pressing grate 3 with tweezers, and the symmetrically distributed pull rings can also realize pressing The grate can be placed on a flat surface smoothly after turning it over 180°.

Embodiment 3: A kind of tissue culture dish assembly of the present embodiment, the similarities with Embodiment 1 will not be repeated, the difference is that, as shown in Figure 6, the size of the air hole 4 on the pressing grate 3 is the same as that of the embodiment. Example 1 is relatively small, with the same size and uniform distribution, which can ensure good air permeability during the tissue block culture process.

Claims (3)

1. a kind of tissue culture dishes component, including by ware lid and ware bottom, which is characterized in that it is provided with and matches suit with ware bottom inner cavity Overlay comb, branch fastener is vertically distributed in the lower surface for overlaying comb, and each branch fastener presses against Min Di inner tissue block upper end respectively, pressure Air hole is distributed on combing in patch.

2. a kind of tissue culture dishes component according to claim 1, which is characterized in that the upper center position for overlaying comb is set It is equipped with card slot and deck has clump weight.

3. a kind of tissue culture dishes component according to claim 2, which is characterized in that the card slot two sides are symmetrically arranged with The pull ring that can be lifted upwards, pull ring are integrally formed with comb is overlayed.