CN202089993U - Cell culture device for imaging of living cells - Google Patents

Cell culture device for imaging of living cells Download PDF

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Publication number
CN202089993U
CN202089993U CN 201120151913 CN201120151913U CN202089993U CN 202089993 U CN202089993 U CN 202089993U CN 201120151913 CN201120151913 CN 201120151913 CN 201120151913 U CN201120151913 U CN 201120151913U CN 202089993 U CN202089993 U CN 202089993U
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China
Prior art keywords
base plate
cavity
cell culture
cavities
substrate
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Expired - Lifetime
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CN 201120151913
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Chinese (zh)
Inventor
刘小龙
杨晓冬
张云
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xiamen Aode Biotechnology Co. Ltd.
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刘小龙
杨晓冬
张云
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Priority to CN 201120151913 priority Critical patent/CN202089993U/en
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Publication of CN202089993U publication Critical patent/CN202089993U/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a cell culture device for imaging of living cells, which comprises a bottom plate, a base plate and a cover plate. The upper surface of the base plate is provided with at least 8 vertically through cavities, the portion surrounding the peripheries of the cavities on the upper surface of the base plate is provided with a plurality of reinforcing ribs, the base plate is fixed on the bottom plate, and the thickness of the bottom is 0.17 millimeter. The cover plate is covered on the cavities, one side of the cover plate is provided with a handle, and the number of the cavities is 8. The portion surrounding the peripheries of the cavities on the lower surface of the base plate is provided with grooves, the base plate is fixedly connected with the bottom plate through adhesive layers arranged in the grooves, the cavities are divided into two rows and arranged on the upper surface of the base plate parallel to each other, and the cavities are in the square shape. The cell culture device is suitable for imaging of living cells with high flux and resolution, firm in structure, convenient for experiment workers to operate and capable of being used for researching a plurality of cells of different experimental conditions in one time.

Description

A kind of cell culture apparatus that is used for the viable cell imaging
Technical field
The utility model relates to cell culture apparatus, is specifically related to a kind of cell culture apparatus that is used for the viable cell imaging.
Background technology
In view of the widespread use of microscopy in Basic of Biology research and medicine industry, various viable cell microscope imaging technology become the important tool in the biomedical research.Go for high-resolution viable cell micro-image, often need to use the microlens (40X, 63X, 100X etc.) of high power, this moment for the thickness of Tissue Culture Dish base material and specific refraction etc. require just to become very harsh.But the correlation parameters such as thickness of at present common cell cultures consumptive material base material all do not satisfy the demand of high-resolution imaging.Make and carry out the very complexity that high resolution viable cell imaging process becomes, often cell need be grown in earlier on the cover glass, then cover glass is fixed in the complicated apparatus system, allow microlens directly carry out imaging in the face of the cover glass sheet.Its process is not only very complicated, consuming time long again, and often being accompanied by nutrient solution leakage, slide glass, may to be exposed to time in the air oversize and influence the function of cell, can not carry out shortcomings such as high-throughput, high-resolution imaging.
Summary of the invention
The utility model provides a kind of cell culture apparatus that is used for the viable cell imaging, this cell culture apparatus is applicable to the research of high-throughput, the imaging of high resolution viable cell, and device has made things convenient for experimenter's operation, has realized disposablely can carrying out parallel study to the cell under a plurality of different test conditionss.
For achieving the above object, the technical solution adopted in the utility model is: a kind of cell culture apparatus that is used for the viable cell imaging comprises: substrate and cover plate, and this upper surface of base plate is provided with the cavity of at least 8 up/down perforations; Described base lower surface and be provided with groove around described cavity peripheral regions, described substrate is fixedlyed connected with described base plate by the binder layer that is positioned at described groove; Described upper surface of base plate and be provided with plurality of strengthening ribs around described cavity peripheral regions; Described substrate is fixed on the described base plate, and this base plate thickness is 0.17mm; Described cover plate is used to cover described cavity top, and this cover plate one side has the leader.
Related content in the technique scheme is explained as follows:
1, in the such scheme, described cavity numbers is 8.
2, in the such scheme, described cavity is divided into two rows and is set in parallel in described upper surface of base plate.
3, in the such scheme, described cavity is square cavity, and this cavity cross-section is long and wide size is 0.8cm*0.8cm.
Because the technique scheme utilization, the utility model compared with prior art has following advantage and effect:
1, the utility model cell culture apparatus is provided with plurality of strengthening ribs and has reinforced cavity, and handle has made things convenient for experimenter's operation.
2, the utility model cell culture apparatus has been realized high-throughput; On a substrate, have 8 cavitys, binder layer and the described base plate adhering junction of substrate by being positioned at described groove, bonding effect is good, prevents the crossed contamination between each cavity, therefore can carry out a plurality of parallel tests simultaneously.Improve flux and speed, improved the efficient of viable cell imaging greatly.
3, the utility model cell culture apparatus cavity cross-section is of a size of 0.8cm*0.8cm, therefore the cultivation liquid that is consumed, need the cell etc. of inoculation also just corresponding reduce a lot, need the 200ul nutrient solution such as each cavity of the utility model, the conventional then minimum cultivation liquid that needs 2ml of consumptive material has been saved consumptive material consumption; And cultured cells can directly be carried out the relevant research of microscope.
Description of drawings
Accompanying drawing 1 is the utility model cell culture apparatus structural representation;
Accompanying drawing 2 is the utility model board structure synoptic diagram.
In the above accompanying drawing: 1, base plate; 2, substrate; 3, cover plate; 4, cavity; 5, handle; 6, stiffening web; 7, groove.
Embodiment
Below in conjunction with drawings and Examples the utility model is further described:
Embodiment: a kind of cell culture apparatus that is used for the viable cell imaging, shown in accompanying drawing 1-2, comprising: base plate 1 also comprises substrate 2 and cover plate 3, these substrate 2 upper surfaces are provided with the cavity 4 of at least 8 up/down perforations, substrate 2 lower surfaces and be provided with groove 7 around described cavity 4 peripheral regions; Described substrate 2 is fixedlyed connected with described base plate 1 by the binder layer that is positioned at described groove 5; Described substrate 2 upper surfaces and be provided with plurality of strengthening ribs 6 around described cavity 4 peripheral regions, described substrate 2 is fixed on the described base plate 1, and this base plate 1 thickness is 0.17mm, and baseboard material is a glass; Described cover plate 3 is used to cover described cavity 4 tops, and these cover plate 3 one sides have leader 5.
Above-mentioned cavity 4 numbers are 8.
Above-mentioned cavity 4 is divided into two rows and is set in parallel in described substrate 2 upper surfaces.
Above-mentioned cavity 4 is square cavity, and these cavity 4 cross sections are long and wide size is 0.8cm*0.8cm.
When adopting above-mentioned cell culture apparatus, be provided with plurality of strengthening ribs and reinforced cavity, and handle has made things convenient for experimenter's operation; Secondly, realize high-throughput, on a substrate, had 8 cavitys, binder layer and the described base plate adhering junction of substrate by being positioned at described groove, bonding effect is good, prevents the crossed contamination between each cavity, therefore can carry out a plurality of parallel tests simultaneously.Improve flux and speed, improved the efficient of viable cell imaging greatly; Once more, cavity cross-section is of a size of 0.8cm*0.8cm, the cultivation liquid that is therefore consumed, needs that the cell etc. of inoculation is also just corresponding to be reduced a lot, needs the 200ul nutrient solution such as each cavity of the utility model, the conventional then minimum cultivation liquid that needs 2ml of consumptive material has been saved consumptive material consumption; And cultured cells is enough carried out the relevant research of microscope.
The foregoing description only is explanation technical conceive of the present utility model and characteristics, and its purpose is to allow the personage who is familiar with this technology can understand content of the present utility model and enforcement according to this, can not limit protection domain of the present utility model with this.All equivalences of being done according to the utility model spirit change or modify, and all should be encompassed within the protection domain of the present utility model.

Claims (4)

1. a cell culture apparatus that is used for the viable cell imaging comprises base plate (1), it is characterized in that: also comprise substrate (2) and cover plate (3), this substrate (2) upper surface is provided with the cavity (4) of at least 8 up/down perforations; Described substrate (2) lower surface and be provided with groove (7) around described cavity (4) peripheral regions, described substrate (2) is fixedlyed connected with described base plate (1) by the binder layer that is positioned at described groove (5); Described substrate (2) upper surface and be provided with plurality of strengthening ribs (6) around described cavity (4) peripheral regions; Described substrate (2) is fixed on the described base plate (1), and this base plate (1) thickness is 0.17mm; Described cover plate (3) is used to cover described cavity (4) top, and these cover plate (3) one sides have leader (5).
2. cell culture apparatus according to claim 1 is characterized in that: described cavity (4) number is 8.
3. cell culture apparatus according to claim 2 is characterized in that: described cavity (4) is divided into two rows and is set in parallel in described substrate (2) upper surface.
4. according to each described cell culture apparatus of claim 1-3, it is characterized in that: described cavity (4) is square cavity, and this cavity (4) cross section is long and wide size is 0.8cm*0.8cm.
CN 201120151913 2011-05-13 2011-05-13 Cell culture device for imaging of living cells Expired - Lifetime CN202089993U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120151913 CN202089993U (en) 2011-05-13 2011-05-13 Cell culture device for imaging of living cells

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Application Number Priority Date Filing Date Title
CN 201120151913 CN202089993U (en) 2011-05-13 2011-05-13 Cell culture device for imaging of living cells

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CN202089993U true CN202089993U (en) 2011-12-28

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102226150A (en) * 2011-05-13 2011-10-26 刘小龙 Cell cultivating apparatus used for imaging living cell
CN106661536A (en) * 2014-05-30 2017-05-10 C.C.M.贝希尔公司 Container for culturing organisms, method for monitoring the culturing of organisms inside said container, and monitoring system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102226150A (en) * 2011-05-13 2011-10-26 刘小龙 Cell cultivating apparatus used for imaging living cell
CN106661536A (en) * 2014-05-30 2017-05-10 C.C.M.贝希尔公司 Container for culturing organisms, method for monitoring the culturing of organisms inside said container, and monitoring system

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C14 Grant of patent or utility model
GR01 Patent grant
ASS Succession or assignment of patent right

Owner name: SUZHOU BEISIMAI MEDICAL INSTRUMENT CO., LTD.

Free format text: FORMER OWNER: LIU XIAOLONG

Effective date: 20121212

Free format text: FORMER OWNER: YANG XIAODONG ZHANG YUN

Effective date: 20121212

C41 Transfer of patent application or patent right or utility model
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Free format text: CORRECT: ADDRESS; FROM: 215513 SUZHOU, JIANGSU PROVINCE TO: 215011 SUZHOU, JIANGSU PROVINCE

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Effective date of registration: 20121212

Address after: 215011 Jiangsu high tech Zone Suzhou City Binhe Road, No. 1326

Patentee after: SUZHOU BEISIMAI MEDICAL INSTRUMENT CO., LTD.

Address before: The Science Park of Changshou City Economic Development Zone, Suzhou City, Jiangsu province 215513 Room 201

Patentee before: Liu Xiaolong

Patentee before: Yang Xiaodong

Patentee before: Zhang Yun

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20170717

Address after: 1809, room 361000, Chuangxin building, Xiamen Industrial Technology Research Institute, 1300 Jimei Road, Jimei District, Fujian, Xiamen

Patentee after: Xiamen Aode Biotechnology Co. Ltd.

Address before: 215011 Jiangsu high tech Zone Suzhou City Binhe Road, No. 1326

Patentee before: SUZHOU BEISIMAI MEDICAL INSTRUMENT CO., LTD.

CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20111228