CN202322857U - Cell culture diagnostic slide for high flux and high-resolution imaging - Google Patents
Cell culture diagnostic slide for high flux and high-resolution imaging Download PDFInfo
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- CN202322857U CN202322857U CN2011204921820U CN201120492182U CN202322857U CN 202322857 U CN202322857 U CN 202322857U CN 2011204921820 U CN2011204921820 U CN 2011204921820U CN 201120492182 U CN201120492182 U CN 201120492182U CN 202322857 U CN202322857 U CN 202322857U
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Abstract
The utility model discloses a cell culture diagnostic slide for high flux and high-resolution imaging, which comprises a glass slide, wherein one end of the glass slide is provided with a tag region and the other end of the glass slide is provided with a cell culture region, a hydrophobic printing ink layer is sprayed on the surface of the cell culture region and is provided with ten through holes which are divided into two rows and are arranged on the hydrophobic printing ink layer in parallel, the quantity of each row of through holes is five; and the diameters of the through holes are 5-7mm, the interval of adjacent through holes is 11-13mm, and the hydrophobic printing ink layer has a width of 20-26mm, a length of 50-60mm and a thickness of 1-1.2mm. A plurality of mutually-independent spaces capable of preventing cross contamination are formed in the glass slide in a partitioning manner, thus the cell culture diagnostic slide realizes a plurality of parallel high-flux cell cultures and subsequent imaging researches under different conditions, and is simple and easy to use; and according to the cell culture diagnostic slide, the efficiency of immunofluorescence staining is greatly increased, and corresponding cell culture materials and the consumption of medicines are reduced.
Description
Technical field
The utility model relates to basic biomedical research and biological medicine industry, is specifically related to a kind of cell cultures seal slide that is used for high-throughput, high-resolution imaging.
Background technology
In view of the widespread use of microscopy in Basic of Biology research and medicine industry, various microscope imaging technology become the important tool in the biomedical research.Especially the immunofluorescence dyeing technology is one of important technical in biology, basic medical and the clinical study.
Common immunofluorescence dyeing is that cell is planted on deckglass earlier at present, and pair cell is fixed and dyeed then, deckglass is fixed on carries out micro-imaging observation on the slide glass at last.But this process is consuming time, each cell culture fluid that consumes and relevant drug consumption be big, when carrying out immunofluorescence dyeing the consumption of consumables associated therewith such as needed antibody also very big.Be unfavorable for practicing thrift consumptive material consumption, shorten the complexity of experimental period and experiment.
Summary of the invention
The utility model provides a kind of cell cultures seal slide that is used for high-throughput, high-resolution imaging; This seal slide is separated out a plurality of spaces separate, that can prevent crossed contamination on slide glass, realized that the parallel high-flux cell under a plurality of different conditions is cultivated and follow-up immunofluorescence dyeing research; And it is simple, easy-to-use to print slide, has improved the efficient of immunofluorescence dyeing greatly, has reduced the consumption of corresponding cell cultures consumptive material and medicine simultaneously.
For achieving the above object, the technical scheme that the utility model adopts is: a kind of cell cultures seal slide that is used for high-throughput and high-resolution imaging comprises slide glass; This slide glass one end is the label area; The other end is the cell cultures district, and this surface, cell cultures district is coated with the hydrophobic ink lay of one deck, and this hydrophobic ink lay is provided with ten through holes; These ten through holes are divided into two rows and are set in parallel on this hydrophobic ink lay, and the number of every exhausting hole is five; The diameter of said through hole is 5 ~ 7mm, is spaced apart 11 ~ 13 mm between the adjacent through-holes, and the width of said hydrophobic ink lay is 20 ~ 26 mm, and length is 50 ~ 60 mm, and thickness is 1 ~ 1.2 mm.
Further improved technical scheme is following in the technique scheme:
As preferred version, the diameter of said through hole is 6mm, is spaced apart 12mm between the adjacent through-holes, and the width of said hydrophobic ink lay is 23 mm, and length is 55mm, and thickness is 1.1 mm.
Because the technique scheme utilization, the utility model compared with prior art has advantage and effect:
1, the utility model is used for the cell cultures seal slide of high-throughput, high-resolution imaging; On slide glass, be separated out a plurality of spaces separate, that can prevent crossed contamination, realized that the parallel high-flux cell under a plurality of different conditions is cultivated and follow-up imaging research; And it is simple, easy-to-use to print slide, has improved the efficient of imaging greatly, has reduced the consumption of corresponding cell cultures consumptive material and medicine simultaneously.
2, the utility model seal slide is provided with the label area and has made things convenient for the experimenter that sample is done sign, is more suitable for microscope imaging research, and cost is low; Realized high-throughput, the seal slide has a plurality of separate holes, and can prevent the crossed contamination between each hole, therefore can carry out a plurality of parallel tests simultaneously.Flux and speed have been improved; The seal slide has been carried out surface treatment, and therefore this seal slide can directly be used for neurocyte, the cultivation of various types of stem cells, and the research of follow-up micro-imaging.
Description of drawings
Accompanying drawing 1 is used for the cell cultures seal slide stereographic map of high-throughput, high-resolution imaging for the utility model;
Accompanying drawing 2 is used for the cell cultures seal slide plane structure chart of high-throughput, high-resolution imaging for the utility model.
In the above accompanying drawing: 1, hydrophobic ink lay; 2, slide glass; 3, label area; 4, cell cultures district; 5, through hole.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further described:
Embodiment: a kind of cell cultures seal slide that is used for high-throughput and high-resolution imaging; Comprise slide glass 2, this slide glass one end is label area 3, and the other end is cell cultures district 4; These 4 surfaces, cell cultures district are coated with the hydrophobic ink lay 1 of one deck; This hydrophobic ink lay 1 is provided with ten through holes 5, and these ten through holes 5 are divided into two rows and are set in parallel on this hydrophobic ink lay 1, and the number of every exhausting hole 5 is five; The diameter of said through hole 5 is 5 ~ 7mm, is spaced apart 11 ~ 13 mm between the adjacent through-holes 5, and the width of said hydrophobic ink lay 1 is 20 ~ 26 mm, and length is 50 ~ 60 mm, and thickness is 1 ~ 1.2 mm.
As preferred version, the diameter of above-mentioned through hole 5 is 6mm, is spaced apart 12mm between the adjacent through-holes 5, and the width of said hydrophobic ink lay 1 is 23 mm, and length is 55mm, and thickness is 1.1 mm.
When adopting the above-mentioned cell cultures that is used for high-throughput, high-resolution imaging to print slide; On slide glass, be separated out a plurality of spaces separate, that can prevent crossed contamination, realized that the parallel high-flux cell under a plurality of different conditions is cultivated and follow-up immunofluorescence dyeing research; And it is simple, easy-to-use to print slide, has improved the efficient of immunofluorescence dyeing greatly, has reduced the consumption of corresponding cell cultures consumptive material and medicine simultaneously; Secondly, be provided with the label area and made things convenient for the experimenter that sample is done sign, be more suitable for microscope imaging research, cost is low; Realized high-throughput, the seal slide has a plurality of separate holes, and can prevent the crossed contamination between each hole, therefore can carry out a plurality of parallel tests simultaneously.Flux and speed have been improved; The seal slide has been carried out surface treatment, and therefore this seal slide can directly be used for neurocyte, the cultivation of various types of stem cells, and the research of follow-up micro-imaging.
The foregoing description only is the technical conceive and the characteristics of explanation the utility model, and its purpose is to let the personage who is familiar with this technology can understand content of the utility model and enforcement according to this, can not limit the protection domain of the utility model with this.All equivalences of being done according to the utility model spirit change or modify, and all should be encompassed within the protection domain of the utility model.
Claims (2)
1. a cell cultures that is used for high-throughput, high-resolution imaging is printed slide; It is characterized in that: comprise slide glass (2), this slide glass one end is label area (3), and the other end is cell cultures district (4); This surface, cell cultures district (4) is coated with the hydrophobic ink lay of one deck (1); This hydrophobic ink lay (1) is provided with ten through holes (5), and these ten through holes (5) are divided into two rows and are set in parallel on this hydrophobic ink lay (1), and the number of every exhausting hole (5) is five; The diameter of said through hole (5) is 5 ~ 7mm, is spaced apart 11 ~ 13 mm between the adjacent through-holes (5), and the width of said hydrophobic ink lay (1) is 20 ~ 26 mm, and length is 50 ~ 60 mm, and thickness is 1 ~ 1.2 mm.
2. seal slide according to claim 1 is characterized in that: the diameter of said through hole (5) is 6mm, is spaced apart 12mm between the adjacent through-holes (5), and the width of said hydrophobic ink lay (1) is 23 mm, and length is 55mm, and thickness is 1.1 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011204921820U CN202322857U (en) | 2011-12-01 | 2011-12-01 | Cell culture diagnostic slide for high flux and high-resolution imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011204921820U CN202322857U (en) | 2011-12-01 | 2011-12-01 | Cell culture diagnostic slide for high flux and high-resolution imaging |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202322857U true CN202322857U (en) | 2012-07-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011204921820U Expired - Fee Related CN202322857U (en) | 2011-12-01 | 2011-12-01 | Cell culture diagnostic slide for high flux and high-resolution imaging |
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| CN (1) | CN202322857U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925353A (en) * | 2012-11-06 | 2013-02-13 | 无锡耐思生物科技有限公司 | Six-hole cultivating plate with glass bottom |
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
| CN111610325A (en) * | 2019-02-25 | 2020-09-01 | 湖州猎源医学检验所有限公司 | Tumor single cell detection kit |
-
2011
- 2011-12-01 CN CN2011204921820U patent/CN202322857U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925353A (en) * | 2012-11-06 | 2013-02-13 | 无锡耐思生物科技有限公司 | Six-hole cultivating plate with glass bottom |
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
| CN111610325A (en) * | 2019-02-25 | 2020-09-01 | 湖州猎源医学检验所有限公司 | Tumor single cell detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: CHEN JIANGMIN ZHANG JIANSONG Effective date: 20140219 Owner name: SUZHOU BEISIMAI MEDICAL INSTRUMENT CO., LTD. Free format text: FORMER OWNER: WANG DONG Effective date: 20140219 |
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| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 215513 SUZHOU, JIANGSU PROVINCE TO: 215011 SUZHOU, JIANGSU PROVINCE |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140219 Address after: 215011 Jiangsu high tech Zone Suzhou City Binhe Road, No. 1326 room 333 Patentee after: SUZHOU BEISIMAI MEDICAL INSTRUMENT CO., LTD. Address before: The four road Changshu Economic Development Zone Suzhou city Jiangsu province 215513 No. 11 Science Park 201 Patentee before: Liu Xiaolong Patentee before: Yang Xiaodong Patentee before: Zhang Yun |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170119 Address after: Cangshan District of Fuzhou City, Fujian province 350000 to build a new town, guanpu Road No. 136 Pu Taijiang Industrial Park No. 8 block No. 26 building third layer Patentee after: Fuzhou Shi Chen Biological Technology Co. Ltd. Address before: 215011 Jiangsu high tech Zone Suzhou City Binhe Road, No. 1326 room 333 Patentee before: SUZHOU BEISIMAI MEDICAL INSTRUMENT CO., LTD. |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120711 Termination date: 20191201 |