CN202322855U - Cell culture device for immunofluorescence staining - Google Patents
Cell culture device for immunofluorescence staining Download PDFInfo
- Publication number
- CN202322855U CN202322855U CN 201120448441 CN201120448441U CN202322855U CN 202322855 U CN202322855 U CN 202322855U CN 201120448441 CN201120448441 CN 201120448441 CN 201120448441 U CN201120448441 U CN 201120448441U CN 202322855 U CN202322855 U CN 202322855U
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- China
- Prior art keywords
- silica gel
- gel sheet
- cell culture
- holes
- utility
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
Abstract
The utility model discloses a cell culture device for immunofluorescence staining. The device comprises a silica gel sheet and a glass slide, wherein eight through holes are formed in the silica gel sheet; the eight through holes are divided into two rows and are arranged in the silica gel sheet in parallel; the quantity of the through holes in each row is four; a glue layer is arranged between the glass slide and the silica gel sheet; another glue layer is arranged on the lower surface of the silica gel sheet; the diameter of each through hole is 4-8mm; an interval between adjacent through holes is 10-14mm; and the silica gel sheet is 20-32mm wide, 40-65mm long and 1-1.2mm thick. The cell culture device provided by the utility model can be closely glued with the glass slide; a plurality of independent spaces capable of preventing cross contamination are separated on the glass slide; the device is simple and easily used; and the efficiency of immunofluorescence staining is greatly increased and the consumption of corresponding cell culture materials and drugs is reduced.
Description
Technical field
The utility model relates to basic biomedical research and biological medicine industry, is specifically related to a kind of cell culture apparatus that is used for immunofluorescence dyeing.
Background technology
In view of the widespread use of microscopy in Basic of Biology research and medicine industry, various microscope imaging technology become the important tool in the biomedical research.Especially the immunofluorescence dyeing technology is one of important technical in biology, basic medical and the clinical study.
Common immunofluorescence dyeing is that cell is planted on deckglass earlier at present, and pair cell is fixed and dyeed then, deckglass is fixed on carries out micro-imaging observation on the slide glass at last.But this process is consuming time, each cell culture fluid that consumes and relevant drug consumption be big, when carrying out immunofluorescence dyeing the consumption of consumables associated therewith such as needed antibody also very big.Be unfavorable for practicing thrift consumptive material consumption, shorten the complexity of experimental period and experiment.
Summary of the invention
The utility model provides a kind of cell culture apparatus that is used for immunofluorescence dyeing, and this cell culture apparatus can be realized and the close adhesion of carrying sheet glass, on slide glass, be separated out a plurality of spaces separate, that can prevent crossed contamination; Simply, easy-to-use, improved the efficient of immunofluorescence dyeing greatly and reduced the consumption of corresponding cell cultures consumptive material and medicine.
For achieving the above object; The technical scheme that the utility model adopts is: a kind of cell culture apparatus that is used for immunofluorescence dyeing, comprise the silica gel sheet, and this silica gel sheet is provided with eight through holes; These eight through holes are divided into two rows and are set in parallel on this silica gel sheet, and the number of every exhausting hole is four; One slide glass is provided with a glue layer between this slide glass and the silica gel sheet; Said silica gel sheet lower surface is provided with a glue layer; The diameter of said through hole is 4 ~ 8mm, is spaced apart 10 ~ 14 mm between the adjacent through-holes, and the width of said silica gel sheet is 20 ~ 32 mm, and length is 40 ~ 65 mm, and thickness is 1 ~ 1.2 mm.
Further improved technical scheme is following in the technique scheme:
As preferred version, the diameter of said through hole is 7.5mm, is spaced apart 12mm between the adjacent through-holes, and the width of said silica gel sheet is 26 mm, and length is 57mm, and thickness is 1.1 mm.
Because the technique scheme utilization, the utility model compared with prior art has advantage and effect:
1, the utility model is used for the cell culture apparatus of immunofluorescence dyeing, can realize and the close adhesion of carrying sheet glass, on slide glass, is separated out a plurality of spaces separate, that can prevent crossed contamination; Simply, easy-to-use, improved the efficient of immunofluorescence dyeing greatly and reduced the consumption of corresponding cell cultures consumptive material and medicine.
2, the utility model is used for the cell culture apparatus of immunofluorescence dyeing, is fit to microscope imaging research more, has reduced cost, has realized high-throughput; The silica gel sheet can be separated out 8 separate space separate, that can prevent crossed contamination on same slide glass, therefore can carry out a plurality of parallel tests simultaneously.Flux and speed have been improved.
Description of drawings
Accompanying drawing 1 is used for the cell culture apparatus schematic perspective view of immunofluorescence dyeing for the utility model;
Accompanying drawing 2 is used for the cell culture apparatus front view of immunofluorescence dyeing for the utility model;
Accompanying drawing 3 is the upward view of accompanying drawing 2.
In the above accompanying drawing: 1, silica gel sheet; 2, through hole; 3, slide glass; 4, glue layer.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further described:
Embodiment: a kind of cell culture apparatus that is used for immunofluorescence dyeing, comprise the silica gel sheet, this silica gel sheet is provided with eight through holes, and these eight through holes 2 are divided into two rows and are set in parallel on this silica gel sheet 1, and the number of every exhausting hole 2 is four; One slide glass is provided with a glue layer 2 between this slide glass 3 and the silica gel sheet 1; Said silica gel sheet 1 lower surface is provided with a glue layer 4; The diameter of said through hole 2 is 4 ~ 8mm, is spaced apart 10 ~ 14 mm between the adjacent through-holes 2, and the width of said silica gel sheet 1 is 20 ~ 32 mm, and length is 40 ~ 65 mm, and thickness is 1 ~ 1.2 mm.
As preferred version, the diameter of above-mentioned through hole 2 is 7.5mm, is spaced apart 12mm between the adjacent through-holes 2, and the width of said silica gel sheet 1 is 26 mm, and length is 57mm, and thickness is 1.1 mm.
When adopting the above-mentioned cell culture apparatus that is used for immunofluorescence dyeing, can realize and the close adhesion of carrying sheet glass, on slide glass, be separated out a plurality of spaces separate, that can prevent crossed contamination; Simply, easy-to-use, improved the efficient of immunofluorescence dyeing greatly and reduced the consumption of corresponding cell cultures consumptive material and medicine; Secondly, the silica gel sheet is fit to microscope imaging research more, has reduced cost, has realized high-throughput; The silica gel sheet can be separated out 8 separate space separate, that can prevent crossed contamination on same slide glass, therefore can carry out a plurality of parallel tests simultaneously.Flux and speed have been improved.
The foregoing description only is the technical conceive and the characteristics of explanation the utility model, and its purpose is to let the personage who is familiar with this technology can understand content of the utility model and enforcement according to this, can not limit the protection domain of the utility model with this.All equivalences of being done according to the utility model spirit change or modify, and all should be encompassed within the protection domain of the utility model.
Claims (2)
1. cell culture apparatus that is used for immunofluorescence dyeing; It is characterized in that: comprise silica gel sheet (1); This is provided with eight through holes (2), and these eight through holes (2) are divided into two rows and are set in parallel on this silica gel sheet (1), and the number of every exhausting hole (2) is four; One slide glass is provided with a glue layer (4) between this slide glass (3) and the silica gel sheet (1); The diameter of said through hole (2) is 4 ~ 8mm, is spaced apart 10 ~ 14 mm between the adjacent through-holes (2), and the width of said silica gel sheet (1) is 20 ~ 32 mm, and length is 40 ~ 65 mm, and thickness is 1 ~ 1.2 mm.
2. cell culture apparatus according to claim 1 is characterized in that: the diameter of said through hole (2) is 7.5mm, is spaced apart 12mm between the adjacent through-holes (2), and the width of said silica gel sheet (1) is 26 mm, and length is 57mm, and thickness is 1.1 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120448441 CN202322855U (en) | 2011-11-14 | 2011-11-14 | Cell culture device for immunofluorescence staining |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120448441 CN202322855U (en) | 2011-11-14 | 2011-11-14 | Cell culture device for immunofluorescence staining |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202322855U true CN202322855U (en) | 2012-07-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120448441 Expired - Fee Related CN202322855U (en) | 2011-11-14 | 2011-11-14 | Cell culture device for immunofluorescence staining |
Country Status (1)
| Country | Link |
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| CN (1) | CN202322855U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424450A (en) * | 2015-12-17 | 2016-03-23 | 天津医科大学总医院 | Floating sphere immunofluorescent staining method and staining device |
| CN106248464A (en) * | 2016-09-02 | 2016-12-21 | 四川大学 | A kind of efficient dyeing apparatus of cell climbing sheet |
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
-
2011
- 2011-11-14 CN CN 201120448441 patent/CN202322855U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424450A (en) * | 2015-12-17 | 2016-03-23 | 天津医科大学总医院 | Floating sphere immunofluorescent staining method and staining device |
| CN105424450B (en) * | 2015-12-17 | 2018-12-18 | 天津医科大学总医院 | Suspension cell ball immunofluorescence dyeing method and dyeing apparatus |
| CN106248464A (en) * | 2016-09-02 | 2016-12-21 | 四川大学 | A kind of efficient dyeing apparatus of cell climbing sheet |
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20161228 Address after: Cangshan District of Fuzhou City, Fujian province 350000 to build a new town, guanpu Road No. 136 Pu Taijiang Industrial Park No. 8 block No. 26 building third layer Patentee after: Fuzhou Shi Chen Biological Technology Co. Ltd. Address before: The four road Changshu Economic Development Zone Suzhou city Jiangsu province 215513 No. 11 Science Park 201 Patentee before: Jiangsu Biomagsystem Medical Co., Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120711 Termination date: 20191114 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |