CN108709991A - A kind of slide and its application method of bovine viral Immunofluorescence test - Google Patents
A kind of slide and its application method of bovine viral Immunofluorescence test Download PDFInfo
- Publication number
- CN108709991A CN108709991A CN201810638302.XA CN201810638302A CN108709991A CN 108709991 A CN108709991 A CN 108709991A CN 201810638302 A CN201810638302 A CN 201810638302A CN 108709991 A CN108709991 A CN 108709991A
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- Prior art keywords
- slide
- top slide
- bovine viral
- immunofluorescence test
- baking vanish
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
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- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of slides and its application method of bovine viral Immunofluorescence test.A kind of slide of bovine viral Immunofluorescence test, including being bonded to each other fixed bottom slide and top slide, the bottom slide and top slide are round rectangle, the top slide is equipped with two exhausting holes, and two exhausting hole interlaced interval setting, the upper surface of the top slide is additionally provided with one piece of baking vanish layer for being used for written record, the baking vanish layer rectangular in form, and the length direction of baking vanish layer and the length direction of top slide are mutually perpendicular to.The present invention uses above-mentioned technical proposal, has the following advantages and feature：1, disposable quantity of sample handling is big；2, compared to traditional culture plate, fluorescence antibody is saved.
Description
Technical field
The present invention relates to a kind of slides and its application method of bovine viral Immunofluorescence test.
Background technology
Generally culture plate is used to carry out bovine viral Immunofluorescence test at present, is tried to the cell specimen after fixation
When testing, due to needing to use expensive fluorescence antibody, if fluorescence antibody can be caused to spread using culture plate at present
Seriously, it not only wastes, and fluorescence antibody is easy to volatilize because thin, influences as a result, leading to error.
Invention content
In order to solve the above technical problems, the first purpose of the invention is to provide it is a kind of it is simple in structure, can reduce it is glimmering
The slide of the bovine viral Immunofluorescence test of photoactivated antibody usage amount.
To achieve the goals above, present invention employs technical solutions below：
A kind of slide of bovine viral Immunofluorescence test, including it is bonded to each other fixed bottom slide and top slide,
The bottom slide and top slide are round rectangle, and the top slide is equipped with two exhausting holes, and two exhausting holes are mutual
Staggeredly interval setting, the upper surface of the top slide are additionally provided with one piece of baking vanish layer for being used for written record, the baking vanish layer at
Rectangular-shaped, and the length direction of baking vanish layer and the length direction of top slide are mutually perpendicular to.
Preferably：The thickness of the bottom slide is 1-1.2mm, and the thickness of the top slide is 0.2-
0.5mm.
Second object of the present invention is to provide a kind of application method of the slide of bovine viral Immunofluorescence test, in order to
Realize the purpose, present invention employs technical solutions below：
A kind of application method of the slide of bovine viral Immunofluorescence test, including above-mentioned slide, and include following step
Suddenly：
1) it, will be digested for the attached cell of Virus culture and be prepared into cell suspension；
2) cell suspension of about 20ul, is added dropwise in the through-hole of top slide, set in 37 DEG C of cell incubators；
3), after cell is adherent, culture medium is gently cleaned with PBS, is dried；
4), slide is set in -20 DEG C of cold acetones and fixes 15min；
5) after, taking out, PBS is cleaned again, is dried
6) about 12-15ul fluorescence antibody dyestuffs, are added dropwise in the through-hole of top slide, are placed in 5- in 37 DEG C of cell incubators
8h；
It 7), can be in fluorescence microscopy microscopic observation after completion fluorescent staining.
The present invention uses above-mentioned technical proposal, has the following advantages and feature：1, disposable quantity of sample handling is big；2, phase
Than traditional culture plate, saving fluorescence antibody, (antibody is expensive, the culture plate each samples of nearly 1ml, and parallel add is done with slide
Get up also just 30ul, differs 33 times)；3, observation is convenient, and fluorescence microscope can be observed, and can only just use inversion glimmering with culture plate
Light microscope；4, it preserves and saves space, a box can fill many slice, thin pieces.
Description of the drawings
Fig. 1 is the structural schematic diagram of the present invention.
Fig. 2 is the structural schematic diagram of top slide of the present invention.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
A kind of slide of bovine viral Immunofluorescence test as depicted in figs. 1 and 2, including it is bonded to each other fixed bottom
Slide 1 and top slide 2, the bottom slide 1 and top slide 2 are round rectangle, and the top slide 2 is equipped with two rows
Through-hole 4, and two exhausting holes, 4 interlaced interval setting, the upper surface of the top slide 2 is additionally provided with one piece and is used for written record
Baking vanish layer 3,3 rectangular in form of baking vanish layer, and the length direction of baking vanish layer 3 and the length direction of top slide 2 are mutual
Vertically.The thickness of the bottom slide 1 is 1-1.2mm, and the thickness of the top slide 2 is 0.2-0.5mm.
A kind of application method of the slide of bovine viral Immunofluorescence test, including above-mentioned slide, and include following step
Suddenly：
1) it, will be digested for the attached cell of Virus culture and be prepared into cell suspension；
2) cell suspension of about 20ul, is added dropwise in the through-hole 4 of top slide 2, set in 37 DEG C of cell incubators；
3), after cell is adherent, culture medium is gently cleaned with PBS, is dried；
4), slide is set in -20 DEG C of cold acetones and fixes 15min；
5) after, taking out, PBS is cleaned again, is dried
6) about 12-15ul fluorescence antibody dyestuffs, are added dropwise in the through-hole 4 of top slide 2, are placed in 37 DEG C of cell incubators
5-8h；
7), can be in fluorescence microscopy microscopic observation after completion fluorescent staining, slide can be in -20 DEG C of long-term preservations.
The top slide of the present invention is bonded to each other with bottom slide so that through-hole on the slide of the top shape on the slide of bottom
At micropore, drop will not be spread by surface tension, save material.
It should be pointed out that above example is only the representative example of the present invention.The present invention can also there are many deformations.It is every
Essence according to the present invention is considered as belonging to this to any simple modification, equivalent change and modification made by above example
The protection domain of invention.
Claims (3)
1. a kind of slide of bovine viral Immunofluorescence test, it is characterised in that：Including being bonded to each other fixed bottom slide (1)
With top slide (2), the bottom slide (1) and top slide (2) are round rectangle, and the top slide (2) is equipped with
Two exhausting holes (4), and the setting of two exhausting holes (4) interlaced interval, the upper surface of the top slide (2) be additionally provided with one piece with
Carry out the baking vanish layer (3) of written record, baking vanish layer (3) rectangular in form, and the length direction of baking vanish layer (3) and top slide
(2) length direction is mutually perpendicular to.
2. a kind of slide of bovine viral Immunofluorescence test according to claim 1, it is characterised in that：The bottom glass
The thickness of piece (1) is 1-1.2mm, and the thickness of the top slide (2) is 0.2-0.5mm.
3. a kind of application method of the slide of bovine viral Immunofluorescence test, which is characterized in that including as described in claim 1
Slide, and include the following steps：
1) it, will be digested for the attached cell of Virus culture and be prepared into cell suspension；
2) cell suspension of about 20ul, is added dropwise in the through-hole (4) of top slide (2), set in 37 DEG C of cell incubators；
3), after cell is adherent, culture medium is gently cleaned with PBS, is dried；
4), slide is set in -20 DEG C of cold acetones and fixes 15min；
5) after, taking out, PBS is cleaned again, is dried
6) about 12-15ul fluorescence antibody dyestuffs, are added dropwise in the through-hole (4) of top slide (2), are placed in 37 DEG C of cell incubators
5-8h；
It 7), can be in fluorescence microscopy microscopic observation after completion fluorescent staining.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810638302.XA CN108709991A (en) | 2018-06-20 | 2018-06-20 | A kind of slide and its application method of bovine viral Immunofluorescence test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810638302.XA CN108709991A (en) | 2018-06-20 | 2018-06-20 | A kind of slide and its application method of bovine viral Immunofluorescence test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108709991A true CN108709991A (en) | 2018-10-26 |
Family
ID=63872816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810638302.XA Withdrawn CN108709991A (en) | 2018-06-20 | 2018-06-20 | A kind of slide and its application method of bovine viral Immunofluorescence test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108709991A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100968063B1 (en) * | 2009-12-02 | 2010-07-08 | 대한민국 | A method of manufacturing antigen slide for immunofluorescence antibody assay and a multivalent antigen slide for simultaneous detection of antibodies against japanese encephalitis virus, west nile virus, yellow fever virus and tick-borne encephalitis virus manufactured by using the same |
| CN202322857U (en) * | 2011-12-01 | 2012-07-11 | 刘小龙 | Cell culture diagnostic slide for high flux and high-resolution imaging |
| CN202322856U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | Cell culture device for high-flux high-resolution immunofluorescent staining |
| CN202322855U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | Cell culture device for immunofluorescence staining |
| CN103091826A (en) * | 2012-12-21 | 2013-05-08 | 中国人民解放军第三军医大学第三附属医院 | Set of glass slides used for carrying out immunohistochemistry staining and storage to tissue slice |
| CN205750100U (en) * | 2016-06-28 | 2016-11-30 | 中国人民解放军军事医学科学院附属医院 | Cell is cultivated and immunocytochemistry dyeing apparatus |
| CN107904283A (en) * | 2017-11-09 | 2018-04-13 | 武汉康录生物技术股份有限公司 | A kind of instant multiprobe hybridized slides and its preparation method and application |
-
2018
- 2018-06-20 CN CN201810638302.XA patent/CN108709991A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100968063B1 (en) * | 2009-12-02 | 2010-07-08 | 대한민국 | A method of manufacturing antigen slide for immunofluorescence antibody assay and a multivalent antigen slide for simultaneous detection of antibodies against japanese encephalitis virus, west nile virus, yellow fever virus and tick-borne encephalitis virus manufactured by using the same |
| CN202322856U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | Cell culture device for high-flux high-resolution immunofluorescent staining |
| CN202322855U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | Cell culture device for immunofluorescence staining |
| CN202322857U (en) * | 2011-12-01 | 2012-07-11 | 刘小龙 | Cell culture diagnostic slide for high flux and high-resolution imaging |
| CN103091826A (en) * | 2012-12-21 | 2013-05-08 | 中国人民解放军第三军医大学第三附属医院 | Set of glass slides used for carrying out immunohistochemistry staining and storage to tissue slice |
| CN205750100U (en) * | 2016-06-28 | 2016-11-30 | 中国人民解放军军事医学科学院附属医院 | Cell is cultivated and immunocytochemistry dyeing apparatus |
| CN107904283A (en) * | 2017-11-09 | 2018-04-13 | 武汉康录生物技术股份有限公司 | A kind of instant multiprobe hybridized slides and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 刘辉等: "牛细小病毒的分离鉴定及在不同细胞中的复制动力学研究" * |
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181026 |
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| WW01 | Invention patent application withdrawn after publication |