CN205750100U - Cell is cultivated and immunocytochemistry dyeing apparatus - Google Patents
Cell is cultivated and immunocytochemistry dyeing apparatus Download PDFInfo
- Publication number
- CN205750100U CN205750100U CN201620662702.0U CN201620662702U CN205750100U CN 205750100 U CN205750100 U CN 205750100U CN 201620662702 U CN201620662702 U CN 201620662702U CN 205750100 U CN205750100 U CN 205750100U
- Authority
- CN
- China
- Prior art keywords
- cell
- microscope slide
- cutting plate
- immunocytochemistry
- dyeing apparatus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of cell to cultivate and immunocytochemistry dyeing apparatus, including microscope slide, also include cutting plate and for overlapping device microscope slide and cutting plate being superimposed together；Cutting plate is provided with several through holes, and through hole is through to microscope slide and the upper surface with microscope slide combines and formed for the culture tank cultivating cell；Pass through this utility model, required cell can be directly cultivated on conventional slide, it is easy in experimentation use phase contrast microscope to observe, and directly can carry out immunocytochemistry (fluorescence) on microscope slide after completing cell cultivation and learn Coloration experiment, one piece of microscope slide can complete the workload of porous plate, cultivate immunocytochemistry dyeing from cell all to complete on one piece of microscope slide, improve conventional efficient, save cell culture medium antibody required with dyeing, and whole process need not loaded down with trivial details cell branching program, ensure cell quantity and quality, guarantee is provided for high-quality immunocyte dyeing picture.
Description
Technical field
This utility model relates to cell and cultivates and immunocytochemistry colouring art, cultivates and thin particularly to a kind of cell
Born of the same parents' immunochemistry dyeing apparatus.
Background technology
Cell is cultivated and immunocytochemistry (fluorescence) dyeing is normal experiment in current biological field, but cellular immunization
Chemistry (fluorescence) dyeing dyeing due to the complex steps related to, traditional method may often be such that first cell is cultivated with 24 or
On the prototype coverslip that 48 orifice plate apertures match, after cell to be done is cultivated, fixing cell carries out chemistry (fluorescence) dyeing；Cause
This process relates to multiple step, often results in that cell on slide is few, loss cell is more in flushing process, and the most serious
Being usually owing to accidentally dropping in operating process, causing being beyond recognition which face has cell, often results in the failure of an experiment；Make simultaneously
When carrying out chemistry (fluorescence) dying operation by the method, usually owing to antibody runs off, dry tablet, use the method must use greatly
Amount antibody, causes unnecessary loss.These unfavorable factors seriously restrict the use that immunocytochemistry (fluorescence) dyes, and improve
One of quality that immunocytochemistry (fluorescence) dyes and efficiency also important content being always cell experiment, a kind of good side
Method, device can save substantial amounts of human and material resources, and accelerate the paces of scientific research.
It is therefore desirable to a kind of cell is cultivated and immunocytochemistry dyeing apparatus, cultivate cellular immunization from cell
Learn dyeing all to complete on one piece of microscope slide, improve conventional efficient, save cell culture medium antibody required with dyeing, and whole
Process need not loaded down with trivial details cell branching program, it is ensured that cell quantity and quality, carries for high-quality immunocyte dyeing picture
Supply to ensure.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of cell to cultivate and immunocytochemistry dyeing apparatus,
Cultivate immunocytochemistry dyeing from cell all to complete on one piece of microscope slide, improve conventional efficient, save cell and cultivate
Base antibody required with dyeing, and whole process need not loaded down with trivial details cell branching program, it is ensured that cell quantity and quality, for high-quality
The immunocyte dyeing picture of amount provides guarantee.
Cell of the present utility model cultivate and immunocytochemistry dyeing apparatus, including microscope slide, also include cutting plate and
For the overlapping device that microscope slide and cutting plate are superimposed together；Described cutting plate is provided with several through holes, and described through hole passes through
Wear to microscope slide and combining with the upper surface of microscope slide and formed for the culture tank cultivating cell.
Further, described overlapping device includes transverse slat and is respectively provided at the stringer board at transverse slat two ends, shape between transverse slat and two stringer boards
Becoming the accommodating area for housing microscope slide and cutting plate, the bottom of described stringer board tilts extension formation and is used for compressing towards accommodating area
The presser feet of microscope slide.
Further, described transverse slat is one-body molded with stringer board.
Further, described transverse slat and stringer board use metal material or elastomeric polystyrene material to make.
Further, described through hole is provided around the flexible rings of its inwall.
Further, the height of described flexible rings is more than the thickness of cutting plate.
Further, described flexible rings uses silica gel material to make.
Further, the lower surface of described microscope slide connects has the labelling for labelling culture tank adaptive with cutting plate to paste
Sheet.
Further, the quantity of described through hole is 6-24.
Further, described microscope slide is rectangular with cutting plate, described overlapping device from microscope slide and the broadside of cutting plate or
Microscope slide and cutting plate are clamped by long limit.
The beneficial effects of the utility model: cell of the present utility model is cultivated and immunocytochemistry dyeing apparatus, carry glass
Sheet is removably connected by overlapping device with cutting plate, and microscope slide is formed multiple for cultivating cell after overlapping with cutting plate
Culture tank, can directly cultivate required cell on conventional slide, it is simple to use phase contrast microscope to carry out in experimentation
Observe, and directly can carry out immunocytochemistry (fluorescence) Coloration experiment after completing cell cultivation on microscope slide,
The workload of porous plate can be completed on one piece of microscope slide, cultivate immunocytochemistry dyeing from cell and all can carry glass at one piece
Complete on sheet, improve conventional efficient, save cell culture medium antibody required with dyeing, and whole process need not loaded down with trivial details cell
Branching program, it is ensured that cell quantity and quality, provides guarantee for high-quality immunocyte dyeing picture；This utility model can
Research for cellular immunization dyeing provides a good platform, it is simple to the quick left-hand seat of new hand uses.
Accompanying drawing explanation
With embodiment, this utility model is further described below in conjunction with the accompanying drawings:
Fig. 1 is structural representation of the present utility model；
Fig. 2 is A-A sectional view in Fig. 1.
Detailed description of the invention
Fig. 1 is structural representation of the present utility model, and Fig. 2 is A-A sectional view in Fig. 1, as shown in the figure: the present embodiment thin
Born of the same parents cultivate and immunocytochemistry dyeing apparatus, including microscope slide 1, also include cutting plate 2 and for by microscope slide 1 and cutting plate
The overlapping device that 2 are superimposed together；Described cutting plate 2 is provided with several through holes 21, described through hole 21 be through to microscope slide 1 and with
The upper surface combination of microscope slide 1 forms the culture tank for cultivating cell；Microscope slide 1 is conventional structure, cutting plate 2 and microscope slide
1 form-fit, overlapping refers to that cutting plate 2 covers on microscope slide 1 and the two justified margin；Cutting plate 2 can be by the pure polyphenyl of hard
Ethylene is made, transparent shape, and thickness is about 5mm；Through hole 21 is rounded, is uniformly arranged on cutting plate 2, and its quantity can root
Depending on needs, the such as quantity of through hole 21 can be 6-24；Microscope slide 1 is with cutting plate 2 by overlapping device removably
Being connected, microscope slide 1 forms multiple culture tank for cultivating cell after overlapping with cutting plate 2, can on conventional slide 1 directly
Cultivate required cell, it is simple to use phase contrast microscope to observe in experimentation, and can be straight after completing cell cultivation
It is connected on microscope slide 1 and carries out immunocytochemistry (fluorescence) Coloration experiment, one piece of microscope slide 1 can complete porous plate
Workload, cultivates immunocytochemistry dyeing from cell and all can complete on one piece of microscope slide 1, improve conventional efficient, save
Cell culture medium antibody required with dyeing, and whole process need not loaded down with trivial details cell branching program, it is ensured that cell quantity and matter
Amount, provides guarantee for high-quality immunocyte dyeing picture.
In the present embodiment, described overlapping device includes transverse slat 31 and is respectively provided at the stringer board 32 at transverse slat 31 two ends, transverse slat 31 with
Being formed for housing microscope slide 1 and the accommodating area of cutting plate 2 between two stringer boards 32, inclines towards accommodating area in the bottom of described stringer board 32
Tiltedly extend the presser feet 33 formed for compressing microscope slide 1；Transverse slat 31 combines after being connected with stringer board 32 and takes the shape of the letter U, described microscope slide 1 with
When cutting plate 2 is rectangular, can arrange two pieces of overlapping devices respectively will from two broadsides (or two long limits) of microscope slide 1 and cutting plate 2
Microscope slide 1 and cutting plate 2 clamp；Described transverse slat 31 can be one-body molded with stringer board 32, to increase the structural strength of overlapping device；Described
Transverse slat 31 and stringer board 32 can use metal material or elastomeric polystyrene material to make.
In the present embodiment, described through hole 21 is provided around the flexible rings 4 of its inwall, and flexible rings 4 can hinder when immunostaining
Only fluid loss, it is preferable that the height of described flexible rings 4 is more than the thickness of cutting plate 2；Described flexible rings 4 preferably employs silica gel
Material is made.
In the present embodiment, the lower surface of described microscope slide 1 connects to be had and the mark for labelling culture tank of cutting plate 2 adaptation
Note paster 5；Labelling paster 5 can be chalker with groove, it is possible to for the transparent paster of zona pellucida grid, logical with cutting plate 2
Hole 21 is corresponding, after being used for immuning tissue's dyeing, determines cell position and labelling during observation.
Finally illustrating, above example is only in order to illustrate the technical solution of the utility model and unrestricted, although ginseng
According to preferred embodiment, this utility model is described in detail, it will be understood by those within the art that, can be to this
The technical scheme of utility model is modified or equivalent, without deviating from objective and the model of technical solutions of the utility model
Enclosing, it all should be contained in the middle of right of the present utility model.
Claims (10)
1. cell cultivate and an immunocytochemistry dyeing apparatus, including microscope slide, it is characterised in that: also include cutting plate and
For the overlapping device that microscope slide and cutting plate are superimposed together；Described cutting plate is provided with several through holes, and described through hole passes through
Wear to microscope slide and combining with the upper surface of microscope slide and formed for the culture tank cultivating cell.
Cell the most according to claim 1 is cultivated and immunocytochemistry dyeing apparatus, it is characterised in that: described overlapping device
Including transverse slat and the stringer board that is respectively provided at transverse slat two ends, formed between transverse slat and two stringer boards for housing microscope slide and cutting plate
Accommodating area, the bottom of described stringer board tilts towards accommodating area to extend the presser feet formed for compressing microscope slide.
Cell the most according to claim 2 cultivate and immunocytochemistry dyeing apparatus, it is characterised in that: described transverse slat with
Stringer board is one-body molded.
Cell the most according to claim 2 cultivate and immunocytochemistry dyeing apparatus, it is characterised in that: described transverse slat and
Stringer board uses metal material or elastomeric polystyrene material to make.
Cell the most according to claim 1 is cultivated and immunocytochemistry dyeing apparatus, it is characterised in that: described through hole sets
There is the flexible rings around its inwall.
Cell the most according to claim 5 is cultivated and immunocytochemistry dyeing apparatus, it is characterised in that: described flexible rings
Height more than the thickness of cutting plate.
Cell the most according to claim 5 is cultivated and immunocytochemistry dyeing apparatus, it is characterised in that: described flexible rings
Employing silica gel material is made.
Cell the most according to claim 1 is cultivated and immunocytochemistry dyeing apparatus, it is characterised in that: described microscope slide
Lower surface connect and have and the labelling paster for labelling culture tank that cutting plate is adaptive.
Cell the most according to claim 1 is cultivated and immunocytochemistry dyeing apparatus, it is characterised in that: described through hole
Quantity is 6-24.
Cell the most according to claim 1 is cultivated and immunocytochemistry dyeing apparatus, it is characterised in that: described load glass
Sheet is rectangular with cutting plate, and microscope slide and cutting plate are pressed from both sides by described overlapping device from microscope slide and the broadside of cutting plate or long limit
Tightly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620662702.0U CN205750100U (en) | 2016-06-28 | 2016-06-28 | Cell is cultivated and immunocytochemistry dyeing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620662702.0U CN205750100U (en) | 2016-06-28 | 2016-06-28 | Cell is cultivated and immunocytochemistry dyeing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205750100U true CN205750100U (en) | 2016-11-30 |
Family
ID=57382302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620662702.0U Expired - Fee Related CN205750100U (en) | 2016-06-28 | 2016-06-28 | Cell is cultivated and immunocytochemistry dyeing apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205750100U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
| CN109085696A (en) * | 2018-08-30 | 2018-12-25 | 厦门大学 | Active somatic cell batch transfer blade and preparation method thereof |
| CN109652311A (en) * | 2018-11-06 | 2019-04-19 | 中南大学湘雅二医院 | A kind of restriction device that cell is directly seeded in glass slide |
| CN110133837A (en) * | 2018-02-08 | 2019-08-16 | 中国科学院微生物研究所 | For microscopical load sample device and its application |
| CN113512496A (en) * | 2021-07-09 | 2021-10-19 | 中国科学院过程工程研究所 | Device for in-situ culture, staining and observation of cells for inverted microscope and use method and application thereof |
-
2016
- 2016-06-28 CN CN201620662702.0U patent/CN205750100U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110133837A (en) * | 2018-02-08 | 2019-08-16 | 中国科学院微生物研究所 | For microscopical load sample device and its application |
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
| CN109085696A (en) * | 2018-08-30 | 2018-12-25 | 厦门大学 | Active somatic cell batch transfer blade and preparation method thereof |
| CN109652311A (en) * | 2018-11-06 | 2019-04-19 | 中南大学湘雅二医院 | A kind of restriction device that cell is directly seeded in glass slide |
| CN113512496A (en) * | 2021-07-09 | 2021-10-19 | 中国科学院过程工程研究所 | Device for in-situ culture, staining and observation of cells for inverted microscope and use method and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161130 Termination date: 20170628 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |