CN202322856U - Cell culture device for high-flux high-resolution immunofluorescent staining - Google Patents
Cell culture device for high-flux high-resolution immunofluorescent staining Download PDFInfo
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- CN202322856U CN202322856U CN 201120448593 CN201120448593U CN202322856U CN 202322856 U CN202322856 U CN 202322856U CN 201120448593 CN201120448593 CN 201120448593 CN 201120448593 U CN201120448593 U CN 201120448593U CN 202322856 U CN202322856 U CN 202322856U
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- cell culture
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- hydrophobic ink
- immunofluorescent staining
- flux
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- 238000004113 cell culture Methods 0.000 title claims abstract description 28
- 238000003125 immunofluorescent labeling Methods 0.000 title abstract 4
- 239000011521 glass Substances 0.000 claims abstract description 19
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 19
- 238000004043 dyeing Methods 0.000 claims description 18
- 238000010166 immunofluorescence Methods 0.000 claims description 18
- 239000003814 drug Substances 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 2
- 238000012864 cross contamination Methods 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000003384 imaging method Methods 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 238000011109 contamination Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 3
- 230000004907 flux Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 238000004381 surface treatment Methods 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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Abstract
The utility model discloses a cell culture device for high-flux high-resolution immunofluorescent staining, which comprises a coverslip and a glass slide, wherein the coverslip is attached on the upper surface of the glass slide when in use; one end of the upper surface of the glass slide is provided with a label area, and the other end is provided with a cell culture area; a hydrophobic ink layer is sprayed on the surface of the cell culture area, and provided with ten through holes; the ten through holes are divided into two rows which are arranged in the hydrophobic ink layer in parallel; the number of through holes in each row is five; the diameter of each through hole is 5-7mm, and the interval between every two adjacent through holes is 11-13mm; and the hydrophobic ink layer is 20-26mm wide, 50-60mm long and 1-1.2mm thick. The glass slide is divided into multiple independent spaces capable of preventing cross contamination, thereby implementing parallel high-flux cell culture and subsequent immunofluorescent staining research under different conditions; and the coverslip is simple and easy to use, thereby greatly enhancing the immunofluorescent staining efficiency and lowering the corresponding consumption of consumable materials and drugs for cell culture.
Description
Technical field
The utility model relates to basic biomedical research and biological medicine industry, is specifically related to a kind of cell culture apparatus that is used for high-throughput and high resolution immunofluorescence dyeing.
Background technology
In view of the widespread use of microscopy in Basic of Biology research and medicine industry, various microscope imaging technology become the important tool in the biomedical research.Especially the immunofluorescence dyeing technology is one of important technical in biology, basic medical and the clinical study.
Common immunofluorescence dyeing is that cell is planted on deckglass earlier at present, and pair cell is fixed and dyeed then, deckglass is fixed on carries out micro-imaging observation on the slide glass at last.But this process is consuming time, each cell culture fluid that consumes and relevant drug consumption be big, when carrying out immunofluorescence dyeing the consumption of consumables associated therewith such as needed antibody also very big.Be unfavorable for practicing thrift consumptive material consumption, shorten the complexity of experimental period and experiment.
Summary of the invention
The utility model provides a kind of cell culture apparatus that is used for high-throughput and high resolution immunofluorescence dyeing; This device is separated out a plurality of spaces separate, that can prevent crossed contamination on slide glass, realized that the parallel high-flux cell under a plurality of different conditions is cultivated and follow-up immunofluorescence dyeing research; And it is simple, easy-to-use to print slide, has improved the efficient of immunofluorescence dyeing greatly, has reduced the consumption of corresponding cell cultures consumptive material and medicine simultaneously.
For achieving the above object, the technical scheme that the utility model adopts is: a kind of cell culture apparatus that is used for high-throughput and high resolution immunofluorescence dyeing, comprise deckglass and slide glass, and during use, deckglass is covered on this slide glass upper surface; Said slide glass upper surface one end is the label area; The other end is the cell cultures district, and this surface, cell cultures district is coated with the hydrophobic ink lay of one deck, and this hydrophobic ink lay is provided with ten through holes; These ten through holes are divided into two rows and are set in parallel on this hydrophobic ink lay, and the number of every exhausting hole is five; The diameter of said through hole is 5～7mm, is spaced apart 11～13mm between the adjacent through-holes, and the width of said hydrophobic ink lay is 20～26mm, and length is 50～60mm, and thickness is 1～1.2mm.
Further improved technical scheme is following in the technique scheme:
As preferred version, the diameter of said through hole is 6mm, is spaced apart 12mm between the adjacent through-holes, and the width of said hydrophobic ink lay is 23mm, and length is 55mm, and thickness is 1.1mm.
Because the technique scheme utilization, the utility model compared with prior art has advantage and effect:
1, the utility model is used for the cell culture apparatus of high-throughput and high resolution immunofluorescence dyeing; On slide glass, be separated out a plurality of spaces separate, that can prevent crossed contamination, realized that the parallel high-flux cell under a plurality of different conditions is cultivated and follow-up immunofluorescence dyeing research; And it is simple, easy-to-use to print slide, has improved the efficient of immunofluorescence dyeing greatly, has reduced the consumption of corresponding cell cultures consumptive material and medicine simultaneously.
2, the utility model cell culture apparatus of being used for high-throughput, high resolution immunofluorescence dyeing is provided with the label area and has made things convenient for the experimenter that sample is done sign, is more suitable for microscope imaging research, and cost is low; Realized high-throughput, the seal slide has a plurality of separate holes, and can prevent the crossed contamination between each hole, therefore can carry out a plurality of parallel tests simultaneously.Flux and speed have been improved; The seal slide has been carried out surface treatment, and therefore this seal slide can directly be used for neurocyte, the cultivation of various types of stem cells, and the research of follow-up micro-imaging.
Description of drawings
Accompanying drawing 1 is the utility model cell culture apparatus stereographic map;
Accompanying drawing 2 is slide configurations figure in the utility model cell culture apparatus;
Accompanying drawing 3 is the utility model seal slide deckglass front view.
In the above accompanying drawing: 1, hydrophobic ink lay; 2, slide glass; 3, label area; 4, cell cultures district; 5, through hole; 6, deckglass.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further described:
Embodiment: a kind of cell culture apparatus that is used for high-throughput, high resolution immunofluorescence dyeing, comprise deckglass 6 and slide glass 2, during use, deckglass is covered on this slide glass 2 upper surfaces; This slide glass upper surface one end is label area 3; The other end is cell cultures district 4; These 4 surfaces, cell cultures district are coated with the hydrophobic ink lay 1 of one deck and form the seal slide; This hydrophobic ink lay 1 is provided with ten through holes 5, and these ten through holes 5 are divided into two rows and are set in parallel on this hydrophobic ink lay 1, and the number of every exhausting hole 5 is five; The diameter of said through hole 5 is 5～7mm, is spaced apart 11～13mm between the adjacent through-holes 5, and the width of said hydrophobic ink lay 1 is 20～26mm, and length is 50～60mm, and thickness is 1～1.2mm.
As preferred version, the diameter of above-mentioned through hole 5 is 6mm, is spaced apart 12mm between the adjacent through-holes 5, and the width of said hydrophobic ink lay 1 is 23mm, and length is 55mm, and thickness is 1.1mm.
When adopting the above-mentioned cell culture apparatus that is used for high-throughput, high resolution immunofluorescence dyeing; On slide glass, be separated out a plurality of spaces separate, that can prevent crossed contamination, realized that the parallel high-flux cell under a plurality of different conditions is cultivated and follow-up immunofluorescence dyeing research; And it is simple, easy-to-use to print slide, has improved the efficient of immunofluorescence dyeing greatly, has reduced the consumption of corresponding cell cultures consumptive material and medicine simultaneously; Secondly, be provided with the label area and made things convenient for the experimenter that sample is done sign, be more suitable for microscope imaging research, cost is low; Realized high-throughput, the seal slide has a plurality of separate holes, and can prevent the crossed contamination between each hole, therefore can carry out a plurality of parallel tests simultaneously.Flux and speed have been improved; The seal slide has been carried out surface treatment, and therefore this seal slide can directly be used for neurocyte, the cultivation of various types of stem cells, and the research of follow-up micro-imaging.
The foregoing description only is the technical conceive and the characteristics of explanation the utility model, and its purpose is to let the personage who is familiar with this technology can understand content of the utility model and enforcement according to this, can not limit the protection domain of the utility model with this.All equivalences of being done according to the utility model spirit change or modify, and all should be encompassed within the protection domain of the utility model.
Claims (2)
1. cell culture apparatus that is used for high-throughput, high resolution immunofluorescence dyeing, it is characterized in that: comprise deckglass (6) and slide glass (2), during use, deckglass is covered on this slide glass (2) upper surface;
Said slide glass (2); This slide glass upper surface one end is label area (3); The other end is cell cultures district (4), and this surface, cell cultures district (4) is coated with the hydrophobic ink lay of one deck (1), and this hydrophobic ink lay (1) is provided with ten through holes (5); These ten through holes (5) are divided into two rows and are set in parallel on this hydrophobic ink lay (1), and the number of every exhausting hole (5) is five; The diameter of said through hole (5) is 5 ~ 7mm, is spaced apart 11 ~ 13 mm between the adjacent through-holes (5), and the width of said hydrophobic ink lay (1) is 20 ~ 26 mm, and length is 50 ~ 60 mm, and thickness is 1 ~ 1.2 mm.
2. cell culture apparatus according to claim 1 is characterized in that: the diameter of said through hole (5) is 6mm, is spaced apart 12mm between the adjacent through-holes (5), and the width of said hydrophobic ink lay (1) is 23 mm, and length is 55mm, and thickness is 1.1 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120448593 CN202322856U (en) | 2011-11-14 | 2011-11-14 | Cell culture device for high-flux high-resolution immunofluorescent staining |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120448593 CN202322856U (en) | 2011-11-14 | 2011-11-14 | Cell culture device for high-flux high-resolution immunofluorescent staining |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202322856U true CN202322856U (en) | 2012-07-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120448593 Expired - Fee Related CN202322856U (en) | 2011-11-14 | 2011-11-14 | Cell culture device for high-flux high-resolution immunofluorescent staining |
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| CN (1) | CN202322856U (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498321A (en) * | 2014-11-06 | 2015-04-08 | 徐小杨 | Egg cells automatic recognition and sorting apparatus |
| CN106248464A (en) * | 2016-09-02 | 2016-12-21 | 四川大学 | A kind of efficient dyeing apparatus of cell climbing sheet |
| WO2017177839A1 (en) * | 2016-04-14 | 2017-10-19 | 清华大学 | Super-hydrophobic micro-pit array chip, preparation method therefor and applications thereof |
| CN107653189A (en) * | 2017-11-07 | 2018-02-02 | 山西大学 | A kind of biological tissue's culture apparatus |
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
| CN109443882A (en) * | 2018-12-27 | 2019-03-08 | 郑州金域临床检验中心有限公司 | A kind of chromosome sectioning device |
| CN111610325A (en) * | 2019-02-25 | 2020-09-01 | 湖州猎源医学检验所有限公司 | Tumor Single Cell Detection Kit |
| CN115219308A (en) * | 2022-08-31 | 2022-10-21 | 复旦大学附属中山医院青浦分院(上海市青浦区中心医院) | A preparation method and imaging method for high-resolution imaging of intact thick biological tissue |
| CN115684574A (en) * | 2022-12-09 | 2023-02-03 | 杭州医学院 | An automatic detection device and method for slides by immunofluorescence method |
| CN117007402A (en) * | 2023-04-07 | 2023-11-07 | 深圳市森盈生物科技有限公司 | Double-hole dyeing glass slide |
-
2011
- 2011-11-14 CN CN 201120448593 patent/CN202322856U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498321A (en) * | 2014-11-06 | 2015-04-08 | 徐小杨 | Egg cells automatic recognition and sorting apparatus |
| CN104498321B (en) * | 2014-11-06 | 2017-03-22 | 徐小杨 | Egg cells automatic recognition and sorting apparatus |
| WO2017177839A1 (en) * | 2016-04-14 | 2017-10-19 | 清华大学 | Super-hydrophobic micro-pit array chip, preparation method therefor and applications thereof |
| CN106248464A (en) * | 2016-09-02 | 2016-12-21 | 四川大学 | A kind of efficient dyeing apparatus of cell climbing sheet |
| CN107653189A (en) * | 2017-11-07 | 2018-02-02 | 山西大学 | A kind of biological tissue's culture apparatus |
| CN108709991A (en) * | 2018-06-20 | 2018-10-26 | 浙江天杭生物科技股份有限公司 | A kind of slide and its application method of bovine viral Immunofluorescence test |
| CN109443882A (en) * | 2018-12-27 | 2019-03-08 | 郑州金域临床检验中心有限公司 | A kind of chromosome sectioning device |
| CN111610325A (en) * | 2019-02-25 | 2020-09-01 | 湖州猎源医学检验所有限公司 | Tumor Single Cell Detection Kit |
| CN115219308A (en) * | 2022-08-31 | 2022-10-21 | 复旦大学附属中山医院青浦分院(上海市青浦区中心医院) | A preparation method and imaging method for high-resolution imaging of intact thick biological tissue |
| CN115684574A (en) * | 2022-12-09 | 2023-02-03 | 杭州医学院 | An automatic detection device and method for slides by immunofluorescence method |
| CN117007402A (en) * | 2023-04-07 | 2023-11-07 | 深圳市森盈生物科技有限公司 | Double-hole dyeing glass slide |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150921 Address after: 1809, room 361000, Chuangxin building, Xiamen Industrial Technology Research Institute, 1300 Jimei Road, Jimei District, Fujian, Xiamen Patentee after: Xiamen Aode Biotechnology Co. Ltd. Address before: The four road Changshu Economic Development Zone Suzhou city Jiangsu province 215513 No. 11 Science Park 201 Patentee before: Jiangsu Biomagsystem Medical Co., Ltd. |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160526 Address after: 361000, three, 4, 1302 Jimei Avenue, Jimei District, Fujian, Xiamen Patentee after: Silverwheat National Science (Xiamen) Biotechnology Co. Ltd. Address before: 1809, room 361000, Chuangxin building, Xiamen Industrial Technology Research Institute, 1300 Jimei Road, Jimei District, Fujian, Xiamen Patentee before: Xiamen Aode Biotechnology Co. Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120711 Termination date: 20191114 |
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| CF01 | Termination of patent right due to non-payment of annual fee |