CN202089991U - Cell culture device with removable cavities - Google Patents
Cell culture device with removable cavities Download PDFInfo
- Publication number
- CN202089991U CN202089991U CN 201120151754 CN201120151754U CN202089991U CN 202089991 U CN202089991 U CN 202089991U CN 201120151754 CN201120151754 CN 201120151754 CN 201120151754 U CN201120151754 U CN 201120151754U CN 202089991 U CN202089991 U CN 202089991U
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- Prior art keywords
- cavities
- cavity
- base plate
- cell culture
- culture apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/44—Multiple separable units; Modules
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Cell Biology (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture device with removable cavities, which comprises a bottom plate, a base plate and a cover plate. At least eight cavities which run through vertically are arranged on the upper surface of the base plate, the cavities are provided with grooves which are arranged on the lower surface of the base plate and surround the peripheral areas of the cavities, the base plate is connected with the bottom plate in an adhered manner via adhesive layers located in the grooves, the cover plate is used for covering on the cavities, the eight cavities are divided into two rows, are parallelly arranged on the upper surface of the base plate and are square cavities, and the lengths and the widths of sections of the cavities are 0.8cm*0.8cm. The cell culture device is applicable to high-flux and high-resolution microscopic imaging studies, is simple and easy in use, and can be used for realizing parallel studies for cells under multiple different test conditions at one step.
Description
Technical field
The utility model relates to cell culture apparatus, is specifically related to the removable cell culture apparatus of a kind of cavity.
Background technology
In view of the widespread use of microscopy in Basic of Biology research and medicine industry, various microscope imaging analytical technologies become the important tool in the biomedical research.Go for high quality, high-resolution micro-image, it is extremely important that parameters such as the bottom thickness of culturing cell consumptive material, specific refraction become.Especially go for high-resolution micro-image, often need to use the microlens (40X, 63X, 100X etc.) of high power; This moment is for the very harshness of wanting changes persuing of requirement, especially thickness and the specific refraction of Tissue Culture Dish base material.Present common cell cultures consumptive material, correlation parameters such as the thickness of its base material and specific refraction all do not satisfy the demand of high-resolution imaging.It is very complicated that feasible process of carrying out high-resolution imaging becomes; Often need earlier cell is grown on the cover glass, then the cell of growing on the cover glass is fixed and dyeed; At last cover glass is encapsulated on the slide glass, allows microlens directly carry out high-resolution imaging in the face of cover glass.But its process is very complicated, consuming time longer, exists and once can only analyze the cell under the condition, and the demand of consumptive materials such as nutrient solution is big, can not carry out imaging analysis to viable cell, can not carry out shortcomings such as high-throughput imaging.
Summary of the invention
The utility model provides a kind of cavity removable cell culture apparatus, and this cell culture apparatus is applicable to the research of high-throughput, high resolution micro-imaging; And install simply, easy-to-use, realized disposablely can carrying out parallel study the cell under a plurality of different test conditionss.
For achieving the above object, the technical solution adopted in the utility model is: the removable cell culture apparatus of a kind of cavity, comprise: base plate, substrate and cover plate, this upper surface of base plate is provided with the cavity of at least 8 up/down perforations, described base lower surface and be provided with groove, of binder layer and the described base plate adhering junction of described substrate around described cavity peripheral regions by being positioned at described groove; Described cover plate is used to cover described cavity top.
Related content in the technique scheme is explained as follows:
1, in the such scheme, described cavity numbers is 8.
2, in the such scheme, described cavity is divided into two rows and is set in parallel in described upper surface of base plate.
3, in the such scheme, described cavity is square cavity, and this cavity cross-section is long and wide size is 0.8cm*0.8cm.
Because the technique scheme utilization, the utility model compared with prior art has following advantage and effect:
1, the utility model cell culture apparatus has been realized high-throughput; On a substrate, have 8 cavitys, binder layer and the described base plate adhering junction of substrate by being positioned at described groove, bonding effect is good, prevents the crossed contamination between each cavity, therefore can carry out a plurality of parallel tests simultaneously.Improve flux and speed, improved the efficient of high-resolution imaging greatly.
2, the utility model cell culture apparatus cavity cross-section is of a size of 0.8cm*0.8cm, therefore the cultivation liquid that is consumed, need the cell of inoculation, and the dosage of needed drug treating etc. also just corresponding reduce a lot, need the 200ul nutrient solution such as each cavity of the utility model, the conventional then minimum cultivation liquid that needs 2ml of consumptive material has been saved consumptive material consumption; And cultured cells can be carried out the relevant research of microscope.
3, the utility model cell culture apparatus, under external force, cavity can freely remove and only stay base plate; Can fix the cell on the culture apparatus base plate after removing, processing such as dyeing; Can make things convenient for pair cell to carry out the research of high resolution micro-imaging then.
Description of drawings
Accompanying drawing 1 is the utility model cell culture apparatus structural representation;
Accompanying drawing 2 is the utility model board structure synoptic diagram.
In the above accompanying drawing: 1, base plate; 2, substrate; 3, cover plate; 4, cavity; 5, groove.
Embodiment
Below in conjunction with drawings and Examples the utility model is further described:
Embodiment: the removable cell culture apparatus of a kind of cavity, shown in accompanying drawing 1-2, comprise: base plate 1, substrate 2 and cover plate 3, these substrate 2 upper surfaces are provided with the cavity 4 of at least 8 up/down perforations, described substrate 2 lower surfaces and be provided with groove 5, of binder layer and described base plate 1 adhering junction of described substrate 2 around described cavity 4 peripheral regions by being positioned at described groove 5; Described cover plate 3 is used to cover described cavity 4 tops.
Above-mentioned cavity 4 numbers are 8.
Above-mentioned cavity 4 is divided into two rows and is set in parallel in described substrate 2 upper surfaces.
Above-mentioned cavity 4 is square cavity, and these cavity 4 cross sections are long and wide size is 0.8cm*0.8cm.
When adopting above-mentioned cell culture apparatus, realize high-throughput, on a substrate, had 8 cavitys, binder layer and the described base plate adhering junction of substrate by being positioned at described groove, bonding effect is good, prevents the crossed contamination between each cavity, therefore can carry out a plurality of parallel tests simultaneously.Improve flux and speed, improved the efficient of micro-imaging greatly; Secondly, cavity cross-section is of a size of 0.8cm*0.8cm, the cultivation liquid that is therefore consumed, needs that the cell etc. of inoculation is also just corresponding to be reduced a lot, needs the 200ul nutrient solution such as each cavity of the utility model, the conventional then minimum cultivation liquid that needs 2ml of consumptive material has been saved consumptive material consumption; And cultured cells can be carried out the relevant research of microscope; Once more, under external force, cavity can freely remove and only stay base plate; Can fix the cell on the culture apparatus base plate after removing, processing such as dyeing.Can carry out the research of high resolution microscope imaging by very convenient pair cell then.
The foregoing description only is explanation technical conceive of the present utility model and characteristics, and its purpose is to allow the personage who is familiar with this technology can understand content of the present utility model and enforcement according to this, can not limit protection domain of the present utility model with this.All equivalences of being done according to the utility model spirit change or modify, and all should be encompassed within the protection domain of the present utility model.
Claims (4)
1. removable cell culture apparatus of cavity, comprise base plate (1), it is characterized in that: also comprise substrate (2) and cover plate (3), this substrate (2) upper surface is provided with the cavity (4) of at least 8 up/down perforations, described substrate (2) lower surface and be provided with groove (5), binder layer and described base plate (1) adhering junction of described substrate (2) around described cavity (4) peripheral regions by being positioned at described groove (5); Described cover plate (3) is used to cover described cavity (4) top.
2. cell culture apparatus according to claim 1 is characterized in that: described cavity (4) number is 8.
3. cell culture apparatus according to claim 2 is characterized in that: described cavity (4) is divided into two rows and is set in parallel in described substrate (2) upper surface.
4. according to each described cell culture apparatus of claim 1-3, it is characterized in that: described cavity (4) is square cavity, and this cavity (4) cross section is long and wide size is 0.8cm*0.8cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120151754 CN202089991U (en) | 2011-05-13 | 2011-05-13 | Cell culture device with removable cavities |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120151754 CN202089991U (en) | 2011-05-13 | 2011-05-13 | Cell culture device with removable cavities |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202089991U true CN202089991U (en) | 2011-12-28 |
Family
ID=45365072
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120151754 Expired - Lifetime CN202089991U (en) | 2011-05-13 | 2011-05-13 | Cell culture device with removable cavities |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202089991U (en) |
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2011
- 2011-05-13 CN CN 201120151754 patent/CN202089991U/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150923 Address after: 1809, room 361000, Chuangxin building, Xiamen Industrial Technology Research Institute, 1300 Jimei Road, Jimei District, Fujian, Xiamen Patentee after: Xiamen Aode Biotechnology Co. Ltd. Address before: Four Changshu road Binjiang Economic Development Zone, Suzhou City, Jiangsu province 215513 No. 11 Branch Park Room 201 Patentee before: Jiangsu Biomagsystem Medical Co., Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20111228 |