CN204111769U - A kind of many cells Dual culture and migration observing device - Google Patents
A kind of many cells Dual culture and migration observing device Download PDFInfo
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- CN204111769U CN204111769U CN201420598426.7U CN201420598426U CN204111769U CN 204111769 U CN204111769 U CN 204111769U CN 201420598426 U CN201420598426 U CN 201420598426U CN 204111769 U CN204111769 U CN 204111769U
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Abstract
The utility model discloses a kind of many cells Dual culture and migration observing device, solve many cells Dual culture in prior art and separate with migration observing device, make to test comparatively troublesome problem.The utility model comprises base plate, middle PDMS culture layer and upper PDMS culture layer, described upper PDMS culture layer fits tightly in described middle PDMS culture layer, described middle PDMS culture layer fits tightly on base plate, described upper PDMS culture layer is provided with the first waste liquid hole, second waste liquid hole, first culture hole and the second culture hole, described second waste liquid hole, through described middle PDMS culture layer is extended with the second culture hole, the first cell migration passage is provided with between described first waste liquid hole and the first culture hole, cell connecting passage is provided with between described first culture hole and the second culture hole, the second cell migration passage is provided with between described second culture hole and the second waste liquid hole.By technique scheme, the utility model well solves the problems of the prior art.
Description
Technical field
The utility model relates to bioengineering field, is specifically related to a kind of many cells Dual culture and migration observing device.
Background technology
Cell is the fundamental unit of vital movement, nearly 20 years, from cell levels, molecular level is developed into the research of cell, from morphologic researchdevelopment to the chemical constitution of submicroscopic structure and molecular structure and each different structure, metabolism and signal transmission.The appearance of microfluidic chip technology, has been regarded as the novel important technological platform required for cell research.But micro-fluidic chip is observed many cells Dual culture and many cells migration and separated in prior art, so just make can not observe many cells cultivation and many cells migration in experimentation simultaneously, also make the cost of testing increase.
Utility model content
In order to solve the problems of the prior art, the technical solution adopted in the utility model is as follows:
A kind of many cells Dual culture and migration observing device, comprise glass film plates, middle PDMS culture layer and upper PDMS culture layer.Described upper PDMS culture layer is placed in PDMS culture layer, and described middle PDMS culture layer is placed on glass film plates.Described upper PDMS culture layer, middle PDMS culture layer, glass film plates are one-body molded.Described upper PDMS culture layer is provided with four through holes, wherein lay respectively at PDMS culture layer both sides, top two holes be the first waste liquid hole and the second waste liquid hole, two middle holes are the first cell culture well and the second cell culture well.Between the first culture hole and the second culture hole, the first concave portions is provided with bottom described upper PDMS culture layer; Between the first culture hole and the first waste liquid hole, 7 the second concave portions be parallel to each other are provided with bottom described upper PDMS culture layer.Second waste liquid hole of described upper PDMS culture layer and the second waste liquid hole extend through described middle PDMS culture layer; Between the second waste liquid hole and the second culture hole, 7 the 3rd concave portions be parallel to each other are provided with bottom described middle PDMS culture layer.Described upper PDMS culture layer and described middle PDMS culture layer one-body molded, formation first cultivation pool and the first waste liquid pool are blocked by middle PDMS culture layer in described first culture hole and the first waste liquid hole; Described first concave portions is blocked by middle PDMS culture layer and is formed cell connecting passage; Being blocked by middle PDMS culture layer of described 7 the second concave portions be parallel to each other is formed with 7 the first cell migration passages.Described middle PDMS culture layer and described glass film plates one-body molded, formation second cultivation pool and the second waste liquid pool are blocked by glass film plates in described second culture hole and the second waste liquid hole; Described 7 the 3rd concave portions be parallel to each other are blocked by glass film plates and are formed with 7 the second cell migration passages.
Particularly, the height of described cell connecting passage is 1.5um, only allows two kinds of intercellular substances to exchange.
Particularly, described first cell migration passage and the second cell migration passage height is all 5um, width is all 8um, the distance between two adjacent first cell migration passages is 6um, and the distance between two adjacent second cell migration passages is 6um.
Particularly, described cell connecting passage height is 1.5um, only allows two kinds of intercellular substances to exchange.
Particularly, described upper PDMS culture layer and middle PDMS culture layer are provided with at least two group first waste liquid holes, the second waste liquid hole, the first culture hole, the second culture hole, the first cell migration passage, the second cell migration passage and cell transfering channel.
The utility model compared with prior art, has the following advantages and beneficial effect:
(1) the utility model structure simple, easy to operate, easily produce.
(2) migration of many cells Dual culture and cell is combined on same device by the utility model in microfluidic chip technology, makes the observation in experimentation more complete and has saved experimental cost.
(3) the utility model can carry out Dual culture and migration observation to many group cells simultaneously simultaneously.
Accompanying drawing explanation
fig. 1 is the utility model sectional view.
Fig. 2 is that embodiment 2 structure separates schematic diagram.
Wherein, the component name that Reference numeral is corresponding is called: PDMS culture layer in 1-, the upper PDMS culture layer of 2-, 3-first waste liquid hole, 4-second waste liquid hole, 5-first culture hole, 6-second culture hole, 7-first cell migration passage, 8-cell connecting passage, 9-second cell migration passage, 10-glass film plates.
Embodiment
the purpose of this utility model is the defect overcoming prior art, provides a kind of many cells Dual culture and migration observing device.
embodiment 1
As shown in Figure 1, a kind of many cells Dual culture and migration observing device, comprise glass film plates 10, middle PDMS culture layer 1 and upper PDMS culture layer 2.Described upper PDMS culture layer 2 is close to and is placed in PDMS culture layer 1, and described middle PDMS culture layer 1 is close to and is placed on glass film plates 10.Described upper PDMS culture layer 2, middle PDMS culture layer 1 and described glass film plates 10 are one-body molded.Described upper PDMS culture layer 2 is provided with four through holes, and two holes wherein laying respectively at PDMS culture layer 2 both sides are the first waste liquid hole 3 and the second waste liquid hole 4, and two middle holes are the first culture hole 5 and the second culture hole 6.Between the first culture hole 5 and the second culture hole 6, the first concave portions is provided with bottom described upper PDMS culture layer 2; Between the first culture hole 5 and the first waste liquid hole 3,7 second concave portions that are parallel to each other are provided with bottom described upper PDMS culture layer 2.Second culture hole 6 of described upper PDMS culture layer 2 and the second waste liquid hole 4 extend through described middle PDMS culture layer 1; Between the second waste liquid hole 4 and the second culture hole 6,7 the 3rd concave portions be parallel to each other are provided with bottom described middle PDMS culture layer 1.Formation first cultivation pool and the first waste liquid pool are blocked by middle PDMS culture layer 1 in described first culture hole 5 and the first waste liquid hole 3; Described first concave portions is blocked by middle PDMS culture layer 1 and is formed cell connecting passage 8; Described 7 the second concave portions be parallel to each other blocked formation 7 the first cell migration passages 7 by middle PDMS culture layer 1.Formation second cultivation pool and the second waste liquid pool are blocked by glass film plates 10 in described second culture hole 6 and the second waste liquid hole 4; Described 7 the 3rd concave portions be parallel to each other are blocked formation 7 the second cell migration passages 9 by glass film plates 10.Preferably, described cell connecting passage 8 is highly 1.5um, only allows two kinds of intercellular substances to exchange.Preferably, described first cell migration passage 7 and the second cell migration passage 9 are highly all 5um, and width is all 8um, and the distance between two adjacent first cell migration passages 7 is 6um, and the distance between two adjacent second cell migration passages 9 is 6um.
During experiment, in many cells Dual culture and migration observing device, pour substratum into.Again A cell is injected described first cultivation pool, B cell injects described second cultivation pool.Material now between A cell and B cell will be exchanged by described cell linkages passage, realizes the Dual culture of A cell and B cell.A cell and B cell also can transfer to A cell waste liquid pool and B cell waste liquid pool respectively by the first cell migration passage and the second cell migration passage, therefore, upper fluorescent mark is done to A cell and B cell, just can examine under a microscope the migration of A cell and B cell.
Between the first culture hole and the first waste liquid hole, be provided with 7 second concave portions that are parallel to each other bottom upper PDMS culture layer in the present embodiment, between the second waste liquid hole and the second culture hole, bottom middle PDMS culture layer, be provided with 7 the 3rd concave portions be parallel to each other.In addition, the second concave portions and the 3rd concave portions also can be any amount.
Embodiment 2
As shown in Figure 2, the difference of the present embodiment and embodiment 1 is, described upper PDMS culture layer 2 and middle PDMS culture layer 1 is provided with the structure described at least two group embodiments 1.
According to above-described embodiment, just the utility model can be realized well.What deserves to be explained is; under prerequisite based on said structure design; for solving same technical problem; even if some making on the utility model are without substantial change or polishing; the essence of the technical scheme adopted is still the same with the utility model, therefore it also should in protection domain of the present utility model.
Claims (8)
1. a many cells Dual culture and migration observing device, it is characterized in that: described many cells Dual culture and migration observing device, comprise base plate, middle PDMS culture layer (1) and upper PDMS culture layer (2), described upper PDMS culture layer (2) fits tightly on described middle PDMS culture layer (1), described middle PDMS culture layer (1) fits tightly on base plate, described upper PDMS culture layer (2) is provided with the first waste liquid hole (3), second waste liquid hole (4), first culture hole (5) and the second culture hole (6), described second waste liquid hole (4), through described middle PDMS culture layer (1) is extended with the second culture hole (6), the first cell migration passage (7) is provided with between described first waste liquid hole (3) and the first culture hole (5), cell connecting passage (8) is provided with between described first culture hole (5) and the second culture hole (6), the second cell migration passage (9) is provided with between described second culture hole (6) and the second waste liquid hole (4).
2. a kind of many cells Dual culture according to claim 1 and migration observing device, is characterized in that: described base plate, middle PDMS culture layer (1) and upper PDMS culture layer (2) are one-body molded.
3. a kind of many cells Dual culture according to claim 2 and migration observing device, it is characterized in that: described upper PDMS culture layer (2) bottom is provided with the first concave portions between the first culture hole (5) and the second culture hole (6), described upper PDMS culture layer (2) bottom is provided with the second concave portions between the first culture hole (5) and the first waste liquid hole (3), and described middle PDMS culture layer (1) bottom is provided with the 3rd concave portions between the second culture hole (6) and the second waste liquid hole (4); Described first concave portions and middle PDMS culture layer (1) are close to and are formed described cell connecting passage (8), described second concave portions and the second culture layer (1) are close to and are formed described first cell migration passage (7), and described 3rd concave portions and base plate are close to and are formed described second cell migration passage (9).
4. a kind of many cells Dual culture according to claim 3 and migration observing device, it is characterized in that: between first culture hole (5) of described upper PDMS culture layer (2) and the second waste liquid hole (4), be provided with at least two the second concave portions be parallel to each other, form at least two described first cell migration passages (7); Be provided with at least two the 3rd concave portions be parallel to each other between second culture hole (6) of described middle PDMS culture layer (1) and the second waste liquid hole (4), form at least two described second cell migration passages (9).
5. a kind of many cells Dual culture according to claim 4 and migration observing device, it is characterized in that: described first cell migration passage (7) and the second cell migration passage (9) are highly all 5um, width is all 8um, distance between two adjacent described first cell migration passages (7) is 6um, and the distance between two adjacent described second cell migration passages (9) is 6um.
6. a kind of many cells Dual culture and migration observing device according to any one of claim 1 ~ 5, is characterized in that: the height of described cell connecting passage (8) is 1.5um.
7. a kind of many cells Dual culture according to claim 6 and migration observing device, is characterized in that: described base plate is glass film plates (10).
8. a kind of many cells Dual culture according to claim 7 and migration observing device, is characterized in that: described upper PDMS culture layer (2) and middle PDMS culture layer (1) are provided with at least two group first waste liquid holes (3), the second waste liquid hole (4), the first culture hole (5), the second culture hole (6), the first cell migration passage (7), the second cell migration passage (9) and cell transfering channel (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420598426.7U CN204111769U (en) | 2014-10-16 | 2014-10-16 | A kind of many cells Dual culture and migration observing device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420598426.7U CN204111769U (en) | 2014-10-16 | 2014-10-16 | A kind of many cells Dual culture and migration observing device |
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| Publication Number | Publication Date |
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| CN204111769U true CN204111769U (en) | 2015-01-21 |
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| CN201420598426.7U Expired - Fee Related CN204111769U (en) | 2014-10-16 | 2014-10-16 | A kind of many cells Dual culture and migration observing device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106214495A (en) * | 2016-07-21 | 2016-12-14 | 刘卫辉 | Jejunal nutrition and functional assessment pipe |
| WO2022116406A1 (en) * | 2020-12-02 | 2022-06-09 | 北京大橡科技有限公司 | Open-type co-culture organ-on-a-chip and use thereof |
-
2014
- 2014-10-16 CN CN201420598426.7U patent/CN204111769U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106214495A (en) * | 2016-07-21 | 2016-12-14 | 刘卫辉 | Jejunal nutrition and functional assessment pipe |
| WO2022116406A1 (en) * | 2020-12-02 | 2022-06-09 | 北京大橡科技有限公司 | Open-type co-culture organ-on-a-chip and use thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150121 Termination date: 20191016 |