CN104630062A - Cover glass fixing card for cell culture - Google Patents

Cover glass fixing card for cell culture Download PDF

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Publication number
CN104630062A
CN104630062A CN201310543290.XA CN201310543290A CN104630062A CN 104630062 A CN104630062 A CN 104630062A CN 201310543290 A CN201310543290 A CN 201310543290A CN 104630062 A CN104630062 A CN 104630062A
Authority
CN
China
Prior art keywords
cover glass
glass
cover
plates
fixing card
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310543290.XA
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Chinese (zh)
Inventor
姚红
李强
李振石
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanxi Medical University
Original Assignee
Shanxi Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanxi Medical University filed Critical Shanxi Medical University
Priority to CN201310543290.XA priority Critical patent/CN104630062A/en
Publication of CN104630062A publication Critical patent/CN104630062A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/46Means for fastening

Abstract

A cover glass fixing card for cell culture is convenient for fixing cover glass, is used in the whole cover glass culture process of cells, reduces damages to the cells on the cover glass, and improves the operation efficiency. The fixing card has a pi-shaped structure formed by three vertical glass plates, two opposite glass plates parallel to each other, contour positions of the two glass plates are respectively provided with horizontal grooves, the two glass plates are respectively provided with horizontal stretching perforated rectangular plates, the third glass plate is vertical to the two glass plates, and the middle of the third glass plate is provided with a square hole. Before the cover glass is used to carry out cell culture, the cover glass is horizontally inserted into the grooves and is disinfected, the fixing card and the cover glass are put in a culture dish, primary or passage cell culture is carried out on the cover glass, and replacement of a culture solution, replacement of the culture dish, inverted microscope observation, cell dyeing and other various operations can be realized by holding the perforated rectangular plates on the fixing card without directly clamping the cover glass by using a tweezer, so damages of an apparatus to the cells on the cover glass are avoided, and the operation efficiency is improved. After all above flow completely ends, the cover glass can be slightly released through the square hole in the middle of the transverse glass plate.

Description

A kind of cover glass fixing catch for cell cultures
Technical field
The invention belongs to experiment apparatus technical field, design a kind of glass and make the cover glass fixing catch for cell cultures being convenient to clipping operation.
Background technology
Many experiments in life science field, particularly relate in the experiment of cell cultures, usually need in culturing process, cell to be inoculated on the cover slip, and when cells grown meets experiment condition, cover glass must be taken out from culture dish operations such as doing the dyeing of further form mensuration, patch clamp.At present, the experimental implementation of being taken out from culture dish by cover glass, typically uses pincet and is picked up by cover glass.But can cause many problems like this: the first, due to the existence of nutrient solution, washings, cover glass contacts usually relatively tightr with culture dish, and the tip of pincet is tapering not, is not easy to be provoked by cover glass smoothly; The second, even if provoke cover glass, if clamp not in time, cover glass very easily falls back in culture dish, is usually stacked in above other cover glass, or cover glass upset occurs and causes cell attachment to face down, and cannot carry out subsequent experimental; 3rd, cover glass positive and negative (namely having cell attachment face and acellular attaching face) naked eyes are not easily recognized, cause reverse side Timeliness coverage upward and not because stir in experimentation, then all follow-up experimental procedures are all in vain, waste of manpower, material resources; 4th, cover glass is clamped by pincet, because local pressure is uneven, easily breaks; 5th, experimentation often needs repeatedly removable cover slide, and the clamping repeatedly of pincet will inevitably cause the cell injury in multiple region, affects experimental result.
In recent years, researcher devised some apparatuses, such as cell cultures pathological staining box (plate), was applicable to a large amount of immunocyte dyeing, solved above-mentioned subproblem, but if sample is less, will cause waste; And in the experiment such as many cell function mensuration, must using cover glass as cell carrier, so quick, the safety holding of cover glass just become a major issue urgently to be resolved hurrily.
Once devised before us a kind of cover glass fetching device ( 200820029858.0), in practical application, solve Similar Problems to a great extent, but still there is following defect: 1. when cover glass is inverted by needs, this apparatus cannot be implemented effectively, can't harm the operation of cell; 2. only rely on a press strip fixed cap slide, fixed effect is still imprecise; 3. this apparatus could can only use when operations such as dyeing, observations, and cell cultivation process cannot use.Based on this, we improve in the practice using cover glass fetching device, subsidize (National Nature fund 30900585, National Nature fund 81100056, Shanxi Province basic research project 2011021038-2) by fund project, devise cover glass fixing catch.
Summary of the invention
The object of this invention is to provide a kind of glass and make the cover glass fixing catch for cell cultures being convenient to clipping operation, to overcome the deficiencies in the prior art.
The technical solution adopted in the present invention is: cover glass fixing catch forms " П " shape by three vertical sheet glass, two relative sheet glass are parallel and equal-height position respectively has a horizontal groove, two sheet glass are the rectangular slab with holes that stretches out of each own level respectively, 3rd surface glass plate is vertical with other two sheet glass, and there is a square opening centre.Before use cover glass carries out cell cultures, cover glass level can be inserted groove, disinfect together, then fixing catch and cover glass are together placed in culture dish, carry out primary or passage cell cultivation on the cover slip, change the various operations such as nutrient solution, replacing culture dish, inverted microscope observation and cell dyeing, all realize by the rectangular slab with holes accommodated in fixing catch, and do not need to carry out direct clipping operation with tweezers to cover glass, avoid apparatus to the damage of the cell on cover glass, improve operation efficiency.If above-mentioned flow process all terminates, then by the square opening of horizontal sheet glass central authorities, cover glass can be released gently, carry out the operations such as gummy mounting or lysing cell.Also the situation that cover glass pros and cons is put upside down can not be there is in experimentation.The specification of cover glass has multiple, the most frequently used about 3 ~ 5 kinds, and the width of fixing catch can design several different size to meet the width of cover glass.But the thickness of groove is generally not more than 0.3mm, the whole height of fixing catch is not more than 6mm.
Compared with the cover glass fetching device that we design before, cover glass fixing catch has following innovation and advantage: 1. fixed up and down by cover glass, and when cell is inverted cultivation by needs, overturn cover slide fixing catch just can easily realize; 2. parallel groove design guarantees that cover glass can not come off; 3. the height of cover glass fixing catch is less than the height of general culture dish, easily can be placed in culture dish together with cover glass, cover glass directly can not be contacted in the whole process of cell cultures, dyeing, the experimental technique such as fixing, easy and simple to handle, reduce the damage to cell.
The invention has the beneficial effects as follows, can fixed cap slide easily, and the whole process that the cover glass being through to whole cell is cultivated, all realize by the rectangular slab with holes accommodated in fixing catch, and do not need to carry out direct clipping operation with tweezers to cover glass, avoid apparatus to the damage of the cell on cover glass, improve operation efficiency.
Accompanying drawing explanation
Fig. 1 is cover glass fixing catch overall schematic: 1 groove, the rectangular slab with holes that 2 levels are stretched out, 3 square openings
Fig. 2 is the schematic diagram after cover glass inserts fixing catch.
Embodiment
For clearly demonstrating the technical characterstic of this programme, below by an embodiment, and by reference to the accompanying drawings, this programme is set forth.
As shown in Figure 1, cover glass fixing catch forms " П " shape by three vertical sheet glass, two relative sheet glass are parallel and its equal-height position respectively has a horizontal connected in star, two sheet glass are the rectangular slab with holes that stretches out of each own level respectively, 3rd surface glass plate is vertical with other two sheet glass, and there is a square opening centre; As shown in Figure 2, by the horizontal groove of cover glass by both sides sheet glass equal altitudes, shift onto in fixing catch, the whole or most of of cover glass are just fixed in cover glass fixing catch relatively, accommodate the rectangular slab with holes that in fixing catch, level is stretched out, cover glass can be put into together with fixing catch or take culture dish out of.

Claims (5)

1. the cover glass fixing catch for cell cultures, it is made up of three vertical contour sheet glass, it is characterized in that: two relative sheet glass are parallel, respectively have the rectangular slab with holes that a horizontal groove and level are stretched out, 3rd surface glass plate is vertical with other two sheet glass, and there is a hole centre.
2. a kind of cover glass fixing catch for cell cultures according to claim 1, it is characterized in that, wherein said hole is square or circular.
3. a kind of cover glass fixing catch for cell cultures according to claim 1, is characterized in that, parallel two sheet glass relatively, respectively have a horizontal groove, is positioned at opposite face and contour.
4. a kind of cover glass fixing catch for cell cultures according to claim 1, is characterized in that, parallel two sheet glass relatively, respectively have the rectangular slab with holes that a level is stretched out, lay respectively at sheet glass top and bottom.
5. a kind of cover glass fixing catch for cell cultures according to claim 1, is characterized in that, the 3rd surface glass plate is all vertical in " П " with parallel two relative sheet glass, and there is a square opening centre.
CN201310543290.XA 2013-11-06 2013-11-06 Cover glass fixing card for cell culture Pending CN104630062A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310543290.XA CN104630062A (en) 2013-11-06 2013-11-06 Cover glass fixing card for cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310543290.XA CN104630062A (en) 2013-11-06 2013-11-06 Cover glass fixing card for cell culture

Publications (1)

Publication Number Publication Date
CN104630062A true CN104630062A (en) 2015-05-20

Family

ID=53209314

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310543290.XA Pending CN104630062A (en) 2013-11-06 2013-11-06 Cover glass fixing card for cell culture

Country Status (1)

Country Link
CN (1) CN104630062A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434273A (en) * 2015-08-06 2017-02-22 三鼎生物科技股份有限公司 Fixing device
WO2018115398A3 (en) * 2016-12-23 2018-08-16 Leica Microsystems Cms Gmbh Holder for a microscope slide, microscope and method for controlling a microscope

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201218787Y (en) * 2008-06-06 2009-04-08 曾勇 Sheet making frame for chromosome
CN201239631Y (en) * 2008-08-05 2009-05-20 姚红 Microscopic glass gripper
CN201429730Y (en) * 2009-07-14 2010-03-24 王秀霞 Novel inspection culture glass sheet
CN201737950U (en) * 2010-07-29 2011-02-09 上海中医药大学 Special experimental apparatus for cell growing on cover glass
CN102422198A (en) * 2009-05-11 2012-04-18 卡尔蔡司公司 Microscopy of an object using a sequence of optical microscopy and particle beam microscopy
CN203668416U (en) * 2013-11-06 2014-06-25 山西医科大学 Cover glass fixing clamp for cell culture

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201218787Y (en) * 2008-06-06 2009-04-08 曾勇 Sheet making frame for chromosome
CN201239631Y (en) * 2008-08-05 2009-05-20 姚红 Microscopic glass gripper
CN102422198A (en) * 2009-05-11 2012-04-18 卡尔蔡司公司 Microscopy of an object using a sequence of optical microscopy and particle beam microscopy
US20120133757A1 (en) * 2009-05-11 2012-05-31 Carl Zeiss Ag Microscopic examination of an object using a sequence of optical microscopy and particle beam microscopy
CN201429730Y (en) * 2009-07-14 2010-03-24 王秀霞 Novel inspection culture glass sheet
CN201737950U (en) * 2010-07-29 2011-02-09 上海中医药大学 Special experimental apparatus for cell growing on cover glass
CN203668416U (en) * 2013-11-06 2014-06-25 山西医科大学 Cover glass fixing clamp for cell culture

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434273A (en) * 2015-08-06 2017-02-22 三鼎生物科技股份有限公司 Fixing device
CN106434273B (en) * 2015-08-06 2018-12-11 三鼎生物科技股份有限公司 Fixed device
WO2018115398A3 (en) * 2016-12-23 2018-08-16 Leica Microsystems Cms Gmbh Holder for a microscope slide, microscope and method for controlling a microscope

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Application publication date: 20150520