CN204222410U - A kind of seal for doing scratch test to cell - Google Patents
A kind of seal for doing scratch test to cell Download PDFInfo
- Publication number
- CN204222410U CN204222410U CN201420687673.4U CN201420687673U CN204222410U CN 204222410 U CN204222410 U CN 204222410U CN 201420687673 U CN201420687673 U CN 201420687673U CN 204222410 U CN204222410 U CN 204222410U
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- cut bar
- seal
- flat bottom
- parallel
- doing
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Abstract
The utility model discloses a kind of seal for doing scratch test to cell, comprise a handle (1), one end of described handle (1) is provided with chassis (2), the bottom surface on described chassis (2) is flat bottom (3), and described flat bottom (3) is provided with cut bar (4).The width of described cut bar (4) is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜.The other end of described handle (1) is provided with round handle (5).The utility model is a kind of aseptic, standardization and the seal for doing scratch test to cell be simple and easy to.
Description
Technical field
The utility model relates to a kind of seal, particularly relates to a kind of seal for doing scratch test to cell.
Background technology
In the RESEARCH ON CELL-BIOLOGY that cell is carried out, often need do scratch test to the cell cultivated in Tissue Culture Plate, detect its diffusivity; But there is no special aseptic, standardized, the easy to use instrument doing scratch experiment at present.Current common method is, hold tight after liquid-transfering gun being connected 10ul liquid transfer gun head with one, on the other hand immobilized-cell culture plate, both hands coordinate, and under eye gaze, streak at the bottom of Tissue Culture Plate fast, as the crow flies, make cut with liquid transfer gun head.This method doing scratch test, the both hands of operator are coordinated, trick coordinate all have higher requirements, operator must have considerable operating experience just can carry out.In operation, easily there is irregularity cut, comprise arc shaped, zigzag, tadpole shape cut, the crude unsharp cut in edge, the cut that respective width differs.It is random to there is very large subjectivity in operation, and each cut exists very large subjectivity in the position (coordinate setting) of Tissue Culture Plate bottom surface: cut number, the distance between cut, angle and intersection point easily all occur relatively large deviation.Scoring operation makes the contiguous cell in cut place be subject to horizontal direction active force, easily causes cell to take off wall.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of aseptic, standardization and the seal for doing scratch test to cell be simple and easy to.
In order to solve the problems of the technologies described above, the seal for doing scratch test to cell that the utility model provides, comprise a handle, one end of described handle is provided with chassis, and the bottom surface on described chassis is flat bottom, and described flat bottom is provided with cut bar.
The width of described cut bar is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜.
The other end of described handle is provided with round handle 5.
Described cut bar is arranged and the cut bar of flat bottom described in decile for warp.
Described cut bar be arranged in parallel and the cut bar of flat bottom described in decile for two warps.
Described cut bar be arranged in parallel and the cut bar of flat bottom described in decile for three warps.
Described cut bar is the cut bar of the flat bottom described in a warp and a parallel right-angled intersection setting and decile.
Described cut bar for two warps be arranged in parallel, parallel square crossing to arrange through two parallel warps and the cut bar of flat bottom described in decile.
Described cut bar for three warps be arranged in parallel, parallel square crossing to arrange through three parallel warps and the cut bar of flat bottom described in decile.
Described cut bar is parallel-laid into groined type through line parallel, two parallels and arranges and the cut bar of flat bottom described in decile for two.
Adopt the seal for doing scratch test to cell of technique scheme, the hand of testing people during use can not touch Tissue Culture Plate inner surface, also can not touch the liquid level in culture plate, in order to avoid cultureed cells is contaminated.Determine the cut bar of flat bottom, also just determine scratch width, Distance geometry angle between cut number and each cut, be convenient to quantize, compare.Do in scoring processes, what seal passed to cell is prone active force at the bottom of a vertical Tissue Culture Plate, can not cause cell detachment near cut.Whole seal sterilization packaging, single use, ensures that seal itself can not contamination of cells.
In sum, the utility model is a kind of aseptic, standardization and the seal for doing scratch test to cell be simple and easy to.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the first cut bar structural representation of the present utility model.
Fig. 3 is the second cut bar structural representation of the present utility model.
Fig. 4 is the third cut bar structural representation of the present utility model.
Fig. 5 is the 4th kind of cut article structural representation of the present utility model.
Fig. 6 is the 5th kind of cut article structural representation of the present utility model.
Fig. 7 is the 6th kind of cut article structural representation of the present utility model.
Fig. 8 is the 7th kind of cut article structural representation of the present utility model.
Detailed description of the invention
Below in conjunction with accompanying drawing, the utility model is described in further detail.
See Fig. 1 and Fig. 2, one end of handle 1 is provided with chassis 2, and the bottom surface on chassis 2 is flat bottom 3, and flat bottom 3 is provided with cut bar 4, and the width of cut bar 4 is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜, cut bar 4 the smooth of the edge, ensures the impression edge polishing formed.Particularly, cut bar 4 is that a warp is arranged and the cut bar of decile flat bottom 3.
Further, the other end of handle 1 is provided with round handle 5, and circle handle 5 is spherical, diameter 3.5cm, surface anti-skid treatment, and convenient grasping, can not come off easily from the hand of operator.Circle handle 5 is marked with arrow, and arrow is parallel with the cut bar 4 on flat bottom 3, indicates the direction of cut bar 4.The high 5cm of handle 1, when ensureing to use, the hand of experiment people can not touch Tissue Culture Plate inner surface, also can not touch the liquid level in culture plate, in order to avoid cultureed cells is contaminated.Chassis 2 is circular, and external diameter size is slightly less than existing Universal Cell culture plate internal diameter, can successfully be covered on Tissue Culture Plate bottom surface by seal.Chassis 2 thickness 0.5cm, has and maintains enough compression strength; Even if firmly with an official seal affixed, the cut bar 4 on seal flat bottom 3 also can not be out of shape.The flat bottom 3 on chassis 2 is very smooth, ensures to leave gap between cut bar 4 and Tissue Culture Plate bottom surface when doing seal cut.If leave gap, may have cell residue, cause dim design, cut is unclean.
Determine the cut bar 4 of flat bottom 3, also just determine scratch width, Distance geometry angle between cut number and each cut, be convenient to quantize, compare.Do in scoring processes, what seal passed to cell is prone active force at the bottom of a vertical Tissue Culture Plate, can not cause cell detachment near cut.Whole seal sterilization packaging, single use, ensures that seal itself can not contamination of cells.
Opening Tissue Culture Plate, if any changing liquid needs, carrying out after cell changes liquid according to a conventional method, by Tissue Culture Plate horizontal in superclean bench face.Open the aseptic packaging of seal, take out seal with the right hand, see the direction of arrow on director circle handle; Left hand immobilized-cell culture plate, the right hand presses arrow direction indication, on seal Vertical dimension lower cover is at the bottom of Tissue Culture Plate.After slightly firmly pressing down, vertically mention seal.The cell of Tissue Culture Plate bottom surface, comes off after being subject to seal extruding.Rinse Tissue Culture Plate gently with PBS, absorb flushing liquor, visible bottom surface forms " one " word pattern clearly.Add corresponding nutrient solution, continue to cultivate by the corresponding cultural method of cell, observe cut healing state.
See Fig. 1 and Fig. 3, one end of handle 1 is provided with chassis 2, and the bottom surface on chassis 2 is flat bottom 3, and flat bottom 3 is provided with cut bar 4, and the width of cut bar 4 is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜, cut bar 4 the smooth of the edge, ensures the impression edge polishing formed.Particularly, cut bar 4 is that two warps be arranged in parallel and the cut bar of decile flat bottom 3.
See Fig. 1 and Fig. 4, one end of handle 1 is provided with chassis 2, and the bottom surface on chassis 2 is flat bottom 3, and flat bottom 3 is provided with cut bar 4, and the width of cut bar 4 is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜, cut bar 4 the smooth of the edge, ensures the impression edge polishing formed.Particularly, cut bar 4 is that two warps be arranged in parallel, a parallel square crossing is arranged and the cut bar of decile flat bottom 3 through two parallel warps.
See Fig. 1 and Fig. 5, one end of handle 1 is provided with chassis 2, and the bottom surface on chassis 2 is flat bottom 3, and flat bottom 3 is provided with cut bar 4, and the width of cut bar 4 is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜, cut bar 4 the smooth of the edge, ensures the impression edge polishing formed.Particularly, cut bar 4 is that three warps be arranged in parallel and the cut bar of decile flat bottom 3.
See Fig. 1 and Fig. 6, one end of handle 1 is provided with chassis 2, and the bottom surface on chassis 2 is flat bottom 3, and flat bottom 3 is provided with cut bar 4, and the width of cut bar 4 is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜, cut bar 4 the smooth of the edge, ensures the impression edge polishing formed.Particularly, cut bar 4 is that three warps be arranged in parallel, a parallel square crossing is arranged and the cut bar of decile flat bottom 3 through three parallel warps.
See Fig. 1 and Fig. 7, one end of handle 1 is provided with chassis 2, and the bottom surface on chassis 2 is flat bottom 3, and flat bottom 3 is provided with cut bar 4, and the width of cut bar 4 is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜, cut bar 4 the smooth of the edge, ensures the impression edge polishing formed.Particularly, cut bar 4 is a warp and a parallel right-angled intersection setting and the cut bar of decile flat bottom 3.
See Fig. 1 and Fig. 8, one end of handle 1 is provided with chassis 2, and the bottom surface on chassis 2 is flat bottom 3, and flat bottom 3 is provided with cut bar 4, and the width of cut bar 4 is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜, cut bar 4 the smooth of the edge, ensures the impression edge polishing formed.Particularly, cut bar 4 is two and is parallel-laid into groined type arranges and the cut bar of decile flat bottom 3 through line parallel, two parallels.
Claims (10)
1. one kind for making the seal of scratch test to cell, it is characterized in that: comprise a handle (1), one end of described handle (1) is provided with chassis (2), the bottom surface on described chassis (2) is flat bottom (3), and described flat bottom (3) is provided with cut bar (4).
2. the seal for doing scratch test to cell according to claim 1, is characterized in that: the width of described cut bar (4) is 1 ㎜ ~ 2 ㎜, and the degree of depth is 3 ㎜ ~ 6 ㎜.
3. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: the other end of described handle (1) is provided with round handle (5).
4. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: described cut bar (4) is arranged and the cut bar of flat bottom (3) described in decile for warp.
5. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: described cut bar (4) be arranged in parallel and the cut bar of flat bottom (3) described in decile for two warps.
6. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: described cut bar (4) be arranged in parallel and the cut bar of flat bottom (3) described in decile for three warps.
7. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: described cut bar (4) is the cut bar of the flat bottom (3) described in a warp and a parallel right-angled intersection setting and decile.
8. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: described cut bar (4) for two warps be arranged in parallel, parallel square crossing to arrange through two parallel warps and the cut bar of flat bottom (3) described in decile.
9. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: described cut bar (4) for three warps be arranged in parallel, parallel square crossing to arrange through three parallel warps and the cut bar of flat bottom (3) described in decile.
10. the seal for doing scratch test to cell according to claim 1 and 2, is characterized in that: described cut bar (4) is parallel-laid into groined type through line parallel, two parallels and arranges and the cut bar of flat bottom (3) described in decile for two.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420687673.4U CN204222410U (en) | 2014-11-17 | 2014-11-17 | A kind of seal for doing scratch test to cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420687673.4U CN204222410U (en) | 2014-11-17 | 2014-11-17 | A kind of seal for doing scratch test to cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204222410U true CN204222410U (en) | 2015-03-25 |
Family
ID=52920540
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420687673.4U Expired - Fee Related CN204222410U (en) | 2014-11-17 | 2014-11-17 | A kind of seal for doing scratch test to cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204222410U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104309342A (en) * | 2014-11-17 | 2015-01-28 | 中南大学湘雅二医院 | Seal applied to scratch test of cell |
| CN105838607A (en) * | 2016-05-27 | 2016-08-10 | 上海臻和生物科技有限公司 | Cell cultivation insertion component for scratch experiments and application of cell cultivation insertion component |
-
2014
- 2014-11-17 CN CN201420687673.4U patent/CN204222410U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104309342A (en) * | 2014-11-17 | 2015-01-28 | 中南大学湘雅二医院 | Seal applied to scratch test of cell |
| CN105838607A (en) * | 2016-05-27 | 2016-08-10 | 上海臻和生物科技有限公司 | Cell cultivation insertion component for scratch experiments and application of cell cultivation insertion component |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150325 Termination date: 20201117 |