CN216998452U

CN216998452U - Cell slide culture apparatus

Info

Publication number: CN216998452U
Application number: CN202220390455.9U
Authority: CN
Inventors: 黄鹏; 常实; 魏波; 赵雅鑫; 谭海龙; 欧阳登洁; 刘绵; 陈佩; 吴敏
Original assignee: Xiangya Hospital of Central South University
Current assignee: Xiangya Hospital of Central South University
Priority date: 2022-02-24
Filing date: 2022-02-24
Publication date: 2022-07-19
Anticipated expiration: 2032-02-24

Abstract

The utility model discloses a cell climbing sheet culture device, which comprises a box body and a matched cover body, wherein the box body and the cover body are sealed and covered; a bracket is arranged on the inner bottom surface of the box body, and a gap area formed by the bracket and the box body is used for moisturizing; a plurality of glass plates are uniformly distributed on the support at intervals, a plurality of cylinders are arrayed on the glass plates, and the cylinders are fixedly connected with the glass plates in a separable mode. The device has multiple functions of cell culture, high-flux cell slide preparation and the like, and overcomes the defects in the traditional cell slide design.

Description

Cell slide culture apparatus

Technical Field

The utility model belongs to the technical field of biological experimental equipment, and particularly relates to a cell climbing sheet culture device.

Background

Cell culture is an essential experimental process in biomedical research, and cell slide is needed when further observing the characteristics of cell morphology, protein expression and the like. In the conventional cell slide, a slide is placed in a culture dish/culture plate, conventional cells are spread before the slide, and after the cells grow on the slide substantially full, the slide is taken out, and after rinsing, residual culture medium and serum are sufficiently removed, and then cell fixation and subsequent experiments are performed. The traditional cell slide technology and the traditional cell slide device have the following defects: since cells generally grow on only one side of the cell slide, all experimental operations are performed on the side containing cells. In the process of immunofluorescence staining operation, researchers are easy to confuse one surface containing cells and one surface not containing cells in the glass sheet due to operation negligence, and the recognition error can cause wrong operation, so that the whole experiment is directly failed; secondly, the slide is easy to slide and difficult to fix; due to surface tension, the bottom surface of the climbing sheet can be tightly attached to the bottom surface of the culture pore plate, the climbing sheet is easily crushed in the process of taking out the climbing sheet, and even if the climbing sheet is not crushed, cells originally full on the climbing sheet can be damaged by tweezers, so that an experimental result is influenced; cells can gather around the slide due to the edge effect, and the central area is sparsely distributed, so that the experimental result is influenced; fifthly, in order to prevent the adjacent holes from being polluted by cells, only one cell culture bottle or device can be used for processing, but most of experiment designs need to process the same cells at different time, or the same stimulation needs to be carried out but the stimulation time is inconsistent, so that only a plurality of culture plates can be used for carrying out experiments, great resource waste is caused, the condition that the cell growth environment is inconsistent can also occur, and certain influence can be caused on the experiment results.

Therefore, it is necessary to provide a new cell-slide culture apparatus to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a cell slide culture device which has multiple functions of cell culture, high-flux cell slide preparation and the like, and overcomes the defects in the traditional cell slide design.

The utility model provides a cell climbing sheet culture device, which comprises: the box comprises a box body and a matched cover body, wherein the box body and the cover body are sealed and covered; the bottom surface in the box body is provided with a bracket, a gap area formed by the bracket and the box body is used for moisturizing, and sterile water or other liquid can be added into the gap area to facilitate the maintenance of humidity required by experiments in the box body; a plurality of glass plates are uniformly distributed on the support at intervals, a plurality of cylinders are arrayed on the glass plates, and the cylinders are fixedly connected with the glass plates in a separable mode.

Furthermore, the cover body is made of opaque materials such as dark hard plastics, and the box body is made of transparent materials such as transparent plastics.

Furthermore, a plurality of sunken grooves are arrayed on the glass plate, are circular grooves and serve as insertion parts of the cylinders, and are effectively sealed.

Furthermore, the box body is rectangular.

Furthermore, one side of the glass plate is provided with a mark area.

According to the cell climbing sheet culture device provided by the utility model, cells directly grow on a glass plate of the device, and are uniformly distributed; links such as plate laying, slide taking and the like are eliminated, the experimental time is saved, and cell slides with good growth state and uniform distribution can be obtained in 4 hours after the cell suspension is added, and the condition of cell slide failure is avoided; a plurality of glass plates can be placed in the device at the same time, a plurality of cell culture devices are arranged on each glass plate, and one device can obtain a plurality of cell crawl sheets, so that the preparation of the cell crawl sheets in large batch is facilitated; the method can be suitable for preparing complex experimental designs such as 'same 1 time point, multiple cells, multiple intervention treatments', 'multiple time points, multiple cells, multiple intervention treatments' and the like; the consistency of intervention conditions can be ensured, and the accuracy of research results is improved; the glass tubes are mutually independent, and integrated slide glass of various cells can be simultaneously prepared and subjected to various intervention treatments on a single glass slide; each cell culture device on the glass plate can be designed to have a diameter of 8mm and a height of 10mm, and the cell suspension required in each device is less than 500 microliters, so that the cell culture reagent, the cell number and the drug intervention cost are greatly saved.

Drawings

FIG. 1 is a schematic diagram of the structure of the device of the present invention.

FIG. 2 is a graph showing the control experiment of the growth state and distribution of cells in the center of the slide after culturing in the conventional device, wherein a and b are the cell growth conditions in the center of the slide after culturing in the conventional device, c is the cell growth conditions in the center of the slide after culturing in the conventional device, and d is the control experiment of the number of cells in a single visual field.

In the figure, a rectangular transparent box body-1, a dark plastic cover body-2, a bracket-3, a glass plate-4, a cylinder-5, a moisturizing area-6 and a marking area-7.

Detailed Description

In a first embodiment, the present invention provides a cell-climbing sheet culture apparatus, as shown in fig. 1, the apparatus comprising: the device comprises a rectangular transparent box body 1, a dark plastic cover body 2, a support 3, a glass plate 4, a cylinder 5, a moisturizing area 6 and a marking area 7, wherein the box body and the cover body are sealed and covered, the length of the box body is 125mm, the width of the box body is 85mm, the height of a peripheral frame is 50mm, the support 3 in the box body consists of two plastic transverse rods and five longitudinal rods, the distance between the support and the edge is 10mm, the height of the support is 10mm, the transverse rods are provided with five longitudinal rods to divide the transverse rods into four equal-width sections, the glass plate 4 is arranged on the equal-width sections, the length of the glass plate is 75mm, one side of the glass plate is provided with the marking area 7 which is processed by frosted or white paint and is 15mm long, the width of the glass plate is 25mm and the thickness of 10mm, eight circular grooves which are uniformly distributed, have the depth of about 5mm and the width of 1mm are prepared on the glass plate by a hot-ironing process and are used as an inserting part of the cylinder 5, the cylinder 5 is a transparent plastic circular tube, the diameter of 10mm, thickness 1mm, high 15mm, during the use, overlap the transparent plastic pipe in the circular groove of glass board, it is fixed and sealed, the dark colour plastics lid of this embodiment, the colour is grey, dark colours such as black, length 125mm, width 85mm, high 15mm, design the structure that caves in all around, make things convenient for the lid to imbed on the transparent square box, embedding degree of depth 2 ~ 3mm, cover behind the lid, be the dark environment in the device, be the dark environment all the time in the use in the device, be applicable to the experimental use that needs light-resistant processing such as fluorescence dyeing, in the use, 4 glass boards can be simultaneously and spaced apart placing on the support, aseptic water or other liquid can be added to the moisturizing area 6 between the support, guarantee that the device keeps warm moist environment in the use.

This device includes 4 parts such as transparent square box bottom, glass board, transparent plastic pipe, dark plastics lid, during the use, installs the transparent plastic pipe on the glass board to place in transparent square box bottom, get proper amount cell suspension again and add in the pipe, can place the cell culture case, take off the pipe after 4 ~ 6 hours and can obtain the cell climbing piece. The method for preparing the traditional cell slide is subverted, is simple, quick and reliable, can simultaneously prepare a plurality of cells, intervenes in a plurality of ways and time points under the condition of ensuring that the experiment conditions are completely consistent, and is suitable for preparing the cell slide in various complex experiment designs.

To explore the differences between the present invention and the conventional slide-climbing device, the same thyroid cancer cell lines were cultured in two devices for verification, see fig. 2. The device only needs to install all functional elements, and the cell suspension liquid is 6 multiplied by 10³The column was filled with 400. mu.l and placed in a cell incubator for culture. The traditional method uses 6-hole plate to make cell slide, after the slide is placed in 6-hole plate, the cell density is 6X 10³2ml of cell suspension, and the rest steps are consistent with the traditional slide preparation method. After 6 hours, 2 device slides were HE stained and the number of cells in a single field was observed under a microscope. Statistical analysis was performed using student's T-test. KnotThe results show that the traditional slide has obvious edge effect, the cells at the edge of the slide are close, but the cells at the central area of the slide are sparse (as shown in fig. 2a and 2 b). The device eliminates the defects of the traditional climbing film mode, the cells are uniformly distributed, and the growth capacity is obviously better than that of the traditional device (as shown in figure 2 c). Statistical analysis revealed that the number of cells in a single field was significantly greater in the device of the utility model than in the conventional method (as shown in figure 2 d). The utility model is simple and reliable in preparing cell climbing tablets, and the growth state and distribution condition of cells are superior to those of the traditional method.

The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. A cell-slide culture device, the device comprising: the box body and the cover body are matched, and the box body and the cover body are sealed and covered; a bracket is arranged on the inner bottom surface of the box body, and a gap area formed by the bracket and the box body is used for moisturizing; a plurality of glass plates are uniformly distributed on the support at intervals, a plurality of cylinders are arrayed on the glass plates, and the cylinders are fixedly connected with the glass plates in a separable mode.

2. The cell-climbing sheet culture apparatus according to claim 1, wherein the cover is made of an opaque material, and the box is made of a transparent material; a plurality of inward concave grooves are arrayed on the glass plate, and the grooves are circular grooves and serve as inserting portions of the cylinders.

3. The cell-slide culturing device according to claim 2, wherein the box body is rectangular.

4. The cell-slide culturing device according to claim 3, wherein a marking area is provided on one side of the glass plate.