CN114369536A - Cell climbing sheet culture device and application method thereof - Google Patents
Cell climbing sheet culture device and application method thereof Download PDFInfo
- Publication number
- CN114369536A CN114369536A CN202210178486.2A CN202210178486A CN114369536A CN 114369536 A CN114369536 A CN 114369536A CN 202210178486 A CN202210178486 A CN 202210178486A CN 114369536 A CN114369536 A CN 114369536A
- Authority
- CN
- China
- Prior art keywords
- cell
- slide
- box body
- culture
- glass plates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000009194 climbing Effects 0.000 title abstract description 14
- 239000011521 glass Substances 0.000 claims abstract description 34
- 238000004113 cell culture Methods 0.000 claims abstract description 9
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 6
- 229920003023 plastic Polymers 0.000 claims description 16
- 239000006285 cell suspension Substances 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 2
- 239000012780 transparent material Substances 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 3
- 238000002360 preparation method Methods 0.000 abstract description 6
- 230000007547 defect Effects 0.000 abstract description 4
- 238000013461 design Methods 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 4
- 230000012010 growth Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000000692 Student's t-test Methods 0.000 description 1
- 208000024770 Thyroid neoplasm Diseases 0.000 description 1
- 239000012578 cell culture reagent Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000010409 ironing Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 201000002510 thyroid cancer Diseases 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/10—Hollow fibers or tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Oncology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cell climbing film culture device and an application method thereof, wherein the device comprises a box body and a matched cover body, wherein the box body and the cover body are sealed and covered; a bracket is arranged on the inner bottom surface of the box body, and a gap area formed by the bracket and the box body is used for moisturizing; a plurality of glass plates are uniformly distributed on the support at intervals, a plurality of cylinders are arrayed on the glass plates, and the cylinders are fixedly connected with the glass plates in a separable mode. The device has multiple functions of cell culture, high-flux cell slide preparation and the like, overcomes the defects in the traditional cell slide design, and can rapidly prepare multiple cell slides at the same time.
Description
Technical Field
The invention belongs to the technical field of biological experimental equipment, and particularly relates to a cell climbing sheet culture device and an application method thereof.
Background
Cell culture is an essential experimental process in biomedical research, and cell slide is needed when further observing the characteristics of cell morphology, protein expression and the like. In the conventional cell slide, a slide is placed in a culture dish/culture plate, conventional cells are spread before the slide, and after the cells grow on the slide substantially full, the slide is taken out, and after rinsing, residual culture medium and serum are sufficiently removed, and then cell fixation and subsequent experiments are performed. The traditional cell slide technology and the traditional cell slide device have the following defects: since cells generally grow on only one side of the cell slide, all experimental operations are performed on the side containing cells. In the process of immunofluorescence staining operation, researchers are easy to confuse one surface containing cells and one surface not containing cells in the glass sheet due to operation negligence, and the recognition error can cause wrong operation, so that the whole experiment is directly failed; secondly, the slide is easy to slide and difficult to fix; due to surface tension, the bottom surface of the climbing sheet can be tightly attached to the bottom surface of the culture pore plate, the climbing sheet is easily crushed in the process of taking out the climbing sheet, and even if the climbing sheet is not crushed, cells originally full on the climbing sheet can be damaged by tweezers, so that an experimental result is influenced; cells can gather around the slide due to the edge effect, and the central area is sparsely distributed, so that the experimental result is influenced; fifthly, in order to prevent the adjacent holes from being polluted by cells, only one cell culture bottle or device can be used for processing, but most of experiment designs need to process the same cells at different time, or the same stimulation needs to be carried out but the stimulation time is inconsistent, so that only a plurality of culture plates can be used for carrying out experiments, great resource waste is caused, the condition that the cell growth environment is inconsistent can also occur, and certain influence can be caused on the experiment results.
Therefore, it is necessary to provide a new cell-slide culture apparatus to solve the above problems.
Disclosure of Invention
The invention aims to provide a cell slide culture device which has multiple functions of cell culture, high-flux cell slide preparation and the like, and overcomes the defects in the traditional cell slide design.
The invention provides a cell climbing sheet culture device, which comprises: the box comprises a box body and a matched cover body, wherein the box body and the cover body are sealed and covered; the bottom surface in the box body is provided with a bracket, a gap area formed by the bracket and the box body is used for moisturizing, and sterile water or other liquid can be added into the gap area to facilitate the maintenance of humidity required by experiments in the box body; a plurality of glass plates are uniformly distributed on the support at intervals, a plurality of cylinders are arrayed on the glass plates, and the cylinders are fixedly connected with the glass plates in a separable mode.
Furthermore, the cover body is made of opaque materials such as dark hard plastics, and the box body is made of transparent materials such as transparent plastics.
Furthermore, a plurality of sunken grooves are arrayed on the glass plate, are circular grooves and serve as insertion parts of the cylinders, and are effectively sealed.
Furthermore, the box body is rectangular.
Furthermore, one side of the glass plate is provided with a mark area.
The invention also aims to provide an application method of the cell slide culture device, which comprises the steps of installing the transparent plastic cylinder on the glass plate, placing the transparent plastic cylinder in the bottom of the transparent square box, adding a proper amount of cell suspension into the cylinder, placing the cylinder in a cell culture box, and taking down the round tube after 4-6 hours to obtain the cell slide.
According to the cell climbing sheet culture device provided by the invention, cells directly grow on a glass plate of the device, and are uniformly distributed; links such as plate laying, slide taking and the like are eliminated, the experimental time is saved, and cell slides with good growth state and uniform distribution can be obtained in 4 hours after the cell suspension is added, and the condition of cell slide failure is avoided; a plurality of glass plates can be placed in the device at the same time, a plurality of cell culture devices are arranged on each glass plate, and one device can obtain a plurality of cell crawl sheets, so that the preparation of the cell crawl sheets in large batch is facilitated; the method can be suitable for preparing complex experimental designs such as 'same 1 time point, multiple cells, multiple intervention treatments', 'multiple time points, multiple cells, multiple intervention treatments' and the like; the consistency of intervention conditions can be ensured, and the accuracy of research results is improved; the glass tubes are mutually independent, and integrated slide glass of various cells can be simultaneously prepared and subjected to various intervention treatments on a single glass slide; each cell culture device on the glass plate can be designed to have a diameter of 8mm and a height of 10mm, and the cell suspension required in each device is less than 500 microliters, so that the cell culture reagent, the cell number and the drug intervention cost are greatly saved.
Drawings
FIG. 1 is a schematic diagram of the structure of the device of the present invention.
FIG. 2 is a comparison experimental chart of cell growth state and distribution of the device of the present invention and a conventional device, wherein a and b are cell growth conditions in the central region of the slide after the culture of the conventional device, c is cell growth conditions in the central region of the slide after the culture of the device, and d is a comparison experimental chart of cell number in a single visual field.
In the figure, a rectangular transparent box body-1, a dark plastic cover body-2, a bracket-3, a glass plate-4, a cylinder-5, a moisturizing area-6 and a marking area-7.
Detailed Description
In a first embodiment, the present invention provides a cell-climbing sheet culture apparatus, as shown in fig. 1, the apparatus comprising: the device comprises a rectangular transparent box body 1, a dark plastic cover body 2, a support 3, a glass plate 4, a cylinder 5, a moisturizing area 6 and a marking area 7, wherein the box body and the cover body are sealed and covered, the length of the box body is 125mm, the width of the box body is 85mm, the height of a peripheral frame is 50mm, the support 3 in the box body consists of two plastic transverse rods and five longitudinal rods, the distance between the support and the edge is 10mm, the height of the support is 10mm, the transverse rods are provided with five longitudinal rods to divide the transverse rods into four equal-width sections, the glass plate 4 is arranged on the equal-width sections, the length of the glass plate is 75mm, one side of the glass plate is provided with the marking area 7 which is processed by frosted or white paint and is 15mm long, the width of the glass plate is 25mm and the thickness of 10mm, eight circular grooves which are uniformly distributed, have the depth of about 5mm and the width of 1mm are prepared on the glass plate by a hot-ironing process and are used as an inserting part of the cylinder 5, the cylinder 5 is a transparent plastic circular tube, the diameter of 10mm, thickness 1mm, high 15mm, during the use, overlap the transparent plastic pipe in the circular ring groove of glass board, it is fixed and sealed, the dark colour plastics lid of this embodiment, the colour is grey, dark colours such as black, length 125mm, width 85mm, height 15mm, design the structure of caving in all around, make things convenient for the lid to imbed on the transparent square box, embedding degree of depth 2 ~ 3mm, after covering the lid, be the dark environment in the device all the time in the use, be applicable to the experimental use that needs light-resistant processing such as fluorescence dyeing, in the use, 4 glass boards can be simultaneously and spaced apart placing on the support, aseptic water or other liquid can be added to the region of moisturizing 6 between the support, guarantee that the device keeps warm and moist environment in the use.
This device includes 4 parts such as transparent square box bottom, glass board, transparent plastic pipe, dark plastics lid, during the use, installs the transparent plastic pipe on the glass board to place in transparent square box bottom, get proper amount cell suspension again and add in the pipe, can place the cell culture case, take off the pipe after 4 ~ 6 hours and can obtain the cell climbing piece. The method for preparing the traditional cell slide is overturned, is simple, quick and reliable, realizes the simultaneous preparation of a plurality of cells, a plurality of interventions and a plurality of time points under the condition of ensuring the complete consistency of experimental conditions, and is suitable for the preparation of the cell slide in various complex experimental designs.
To investigate the differences between the present invention and the conventional slide apparatus, the same thyroid cancer cell lines were cultured in two apparatuses, respectively, for verification, see fig. 2. The device only needs to install all functional elements, and the cell suspension liquid is 6 multiplied by 103The column was filled with 400. mu.l and placed in a cell incubator for culture. The traditional method uses 6-hole plate to make cell slide, after the slide is placed in 6-hole plate, the cell density is 6X 1032ml of cell suspension, and the rest steps are consistent with the traditional slide preparation method. After 6 hours, the slide of 2 devices was taken for HE staining and microscopically examinedThe number of cells in a single field was observed under a mirror. Statistical analysis was performed using student's T-test. The results show that the traditional slide has obvious edge effect, the cells at the edge of the slide are close, but the cells at the central area of the slide are sparse (as shown in fig. 2a and 2 b). The device eliminates the defects of the traditional climbing film mode, the cells are uniformly distributed, and the growth capacity is obviously better than that of the traditional device (as shown in figure 2 c). Statistical analysis revealed that the number of cells in a single field of view in the device of the invention was significantly greater than in the conventional method (as shown in figure 2 d). The invention is simple and reliable in preparing cell climbing tablets, and the growth state and distribution condition of cells are superior to those of the traditional method.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (5)
1. A cell-slide culture device, the device comprising: the box body and the cover body are matched, and the box body and the cover body are sealed and covered; a bracket is arranged on the inner bottom surface of the box body, and a gap area formed by the bracket and the box body is used for moisturizing; a plurality of glass plates are uniformly distributed on the support at intervals, a plurality of cylinders are arrayed on the glass plates, and the cylinders are fixedly connected with the glass plates in a separable mode.
2. The cell-slide culturing device according to claim 1, wherein the cover is made of a light-proof material, such as dark hard plastic, and the box is made of a transparent material, such as transparent plastic. Furthermore, a plurality of sunken grooves are arrayed on the glass plate, and the grooves are circular grooves and serve as inserting portions of the cylinder.
3. The cell-slide culturing device according to claim 2, wherein the box body is rectangular.
4. The cell-slide culturing device according to claim 3, wherein a marking region is provided on one side of the glass plate.
5. A method for using the cell slide culture device according to any one of claims 1 to 4, wherein the cell slide is obtained by mounting a transparent plastic cylinder on a glass plate, placing the cylinder in the bottom of a transparent square box, adding a proper amount of cell suspension into the cylinder, placing a cell culture box, and removing the round tube after 4 to 6 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210178486.2A CN114369536A (en) | 2022-02-24 | 2022-02-24 | Cell climbing sheet culture device and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210178486.2A CN114369536A (en) | 2022-02-24 | 2022-02-24 | Cell climbing sheet culture device and application method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114369536A true CN114369536A (en) | 2022-04-19 |
Family
ID=81146775
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210178486.2A Pending CN114369536A (en) | 2022-02-24 | 2022-02-24 | Cell climbing sheet culture device and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114369536A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2835983Y (en) * | 2005-05-30 | 2006-11-08 | 中国人民解放军第四军医大学 | Cell slide pathological treatment wet box device |
| CN201065406Y (en) * | 2007-06-29 | 2008-05-28 | 彭阳慧 | Cell rapid sheet-climbing dyeing box |
| CN201424477Y (en) * | 2009-05-08 | 2010-03-17 | 泸州医学院 | Integrated preparation box for cell and cell climbing sheet |
| KR20120079958A (en) * | 2011-01-06 | 2012-07-16 | 주식회사 에스피엘 | Unit for cell culturing |
| CN202415570U (en) * | 2011-12-30 | 2012-09-05 | 泸州医学院 | Integrated preparation rack for cell growing on glass slide |
| CN203999635U (en) * | 2014-07-17 | 2014-12-10 | 天津医科大学总医院 | Detachable cell climbing sheet culturing plate |
| CN208136266U (en) * | 2018-02-22 | 2018-11-23 | 中国农业科学院特产研究所 | A kind of dedicated culture mechanism of cell climbing sheet and cell culture system |
| CN113640089A (en) * | 2021-09-17 | 2021-11-12 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
| CN215975862U (en) * | 2021-08-16 | 2022-03-08 | 合肥源恩生物技术有限公司 | Cell climbing device |
| CN216998452U (en) * | 2022-02-24 | 2022-07-19 | 中南大学湘雅医院 | Cell slide culture apparatus |
| CN217535989U (en) * | 2022-05-07 | 2022-10-04 | 伊莱瑞特(武汉)生物技术有限公司 | Cell climbing device |
-
2022
- 2022-02-24 CN CN202210178486.2A patent/CN114369536A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2835983Y (en) * | 2005-05-30 | 2006-11-08 | 中国人民解放军第四军医大学 | Cell slide pathological treatment wet box device |
| CN201065406Y (en) * | 2007-06-29 | 2008-05-28 | 彭阳慧 | Cell rapid sheet-climbing dyeing box |
| CN201424477Y (en) * | 2009-05-08 | 2010-03-17 | 泸州医学院 | Integrated preparation box for cell and cell climbing sheet |
| KR20120079958A (en) * | 2011-01-06 | 2012-07-16 | 주식회사 에스피엘 | Unit for cell culturing |
| CN202415570U (en) * | 2011-12-30 | 2012-09-05 | 泸州医学院 | Integrated preparation rack for cell growing on glass slide |
| CN203999635U (en) * | 2014-07-17 | 2014-12-10 | 天津医科大学总医院 | Detachable cell climbing sheet culturing plate |
| CN208136266U (en) * | 2018-02-22 | 2018-11-23 | 中国农业科学院特产研究所 | A kind of dedicated culture mechanism of cell climbing sheet and cell culture system |
| CN215975862U (en) * | 2021-08-16 | 2022-03-08 | 合肥源恩生物技术有限公司 | Cell climbing device |
| CN113640089A (en) * | 2021-09-17 | 2021-11-12 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
| CN216998452U (en) * | 2022-02-24 | 2022-07-19 | 中南大学湘雅医院 | Cell slide culture apparatus |
| CN217535989U (en) * | 2022-05-07 | 2022-10-04 | 伊莱瑞特(武汉)生物技术有限公司 | Cell climbing device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6043027A (en) | Multi-well single-membrane permeation device and methods | |
| US5972694A (en) | Multi-well plate | |
| US9315768B2 (en) | Biological microfluidics chip and related methods | |
| US4299920A (en) | Biological receptacle | |
| KR101446526B1 (en) | Cell culture analyzing device based on microfluidic multi-well format | |
| US7481980B2 (en) | Device for staining and hybridization reactions | |
| US20100029000A1 (en) | Assembly and methods for cell or tissue culture and treatment | |
| CN216998452U (en) | Cell slide culture apparatus | |
| EP1893742B1 (en) | Cell and tissue culture device | |
| CN102719352A (en) | Cell chip slide for preparing microarray cell chips and preparation method | |
| CN103710263A (en) | Cell culture apparatus | |
| US8664005B2 (en) | Method for introducing and transferring multiple minute quantity samples | |
| CN114369536A (en) | Cell climbing sheet culture device and application method thereof | |
| EP1379622B1 (en) | Method and device for cultivating and/or distributing particles | |
| US20230256449A1 (en) | Micro-droplet dish | |
| CN115948339A (en) | Method for culturing glioma organoid by high-flux gas-liquid boundary method | |
| CN112710528B (en) | Sister chromatid interchange differential staining auxiliary device and method | |
| CN112646713A (en) | Chip for integrated tumor cell behavior experiment | |
| CN204417516U (en) | The special Micro-CPE neutralization test ware of laser scanning co-focusing microscope | |
| CN218710460U (en) | Multifunctional cell migration detection device | |
| US20200384466A1 (en) | Loading and sealing sample on microfabricated chip | |
| CN220643142U (en) | Micro imaging tray for organoid chip | |
| WO1997016717A1 (en) | Automated permeability analysis system | |
| CN220413416U (en) | Organoid culture bracket and culture plate for culturing organoid by using matrigel | |
| CZ301758B6 (en) | Method of addition, removal or replacement of liquid medium when culturing biological cells and culturing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |