CN215906197U - Device for chromosome aberration test of in-vitro mammalian cell - Google Patents
Device for chromosome aberration test of in-vitro mammalian cell Download PDFInfo
- Publication number
- CN215906197U CN215906197U CN202122169132.5U CN202122169132U CN215906197U CN 215906197 U CN215906197 U CN 215906197U CN 202122169132 U CN202122169132 U CN 202122169132U CN 215906197 U CN215906197 U CN 215906197U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- culture
- glass slide
- mammalian cells
- plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a device for in vitro mammal cell chromosome aberration test, which comprises a culture dish with a rectangular flat box structure and an open upper end; the culture dish is internally and longitudinally distributed with a first clapboard and a second clapboard in a crisscross way so as to divide the interior of the culture dish into a plurality of culture units which are mutually independent and are used for containing culture solution; the upper surfaces of the first clapboard and the second clapboard are positioned below the end surface of the open end of the culture dish; the culture dish is internally provided with a glass slide frame, the glass slide frame comprises a support plate which is horizontally arranged above the first partition plate and the second partition plate, the support plate is respectively provided with a connecting frame which is arranged downwards corresponding to the culture unit, the lower end of the connecting frame is connected with a horizontal support sheet, a placing station is formed above the support sheet and used for placing a glass slide, and the upper surface of the glass slide forms a culture surface and is used for culturing mammalian cells. The method has the advantages of convenient use, better simplification of operation steps and better application to cell chromosome aberration tests.
Description
Technical Field
The utility model relates to the technical field of cell chromosome aberration test devices, in particular to a device for in-vitro mammal cell chromosome aberration test.
Background
The in vitro mammal cell chromosome aberration test is one of basic tests of a genetic toxicity test standard combination, and the specific operation method comprises the following steps: inoculating mammalian cells with proper concentration to grow to a logarithmic growth phase in a container, adding a test substance under the condition of adding or not adding a metabolic activation system, exposing the cultured mammalian cells to a test sample for a certain time, treating the cells with a metaphase mitosis blocker colchicine to stop the cells at the metaphase mitosis, then harvesting the cells, performing hypotonic treatment on the cells, adding a fixing solution for fixing, then performing drop-plating and staining, and analyzing chromosome aberration. And (3) observing the chromosome aberration condition of the cells of the test sample group in the dyeing piece, thereby evaluating the mutagenicity of the test sample. The conventional method is to inoculate cells in a cell culture bottle, after the sample adding step is completed, the cells need to be harvested into a centrifugal tube, then hypotonic and fixed treatment is carried out, and after centrifugation, dropping and staining are carried out, so that the operation steps in the whole process are more.
Therefore, how to provide a device for in vitro mammalian cell chromosome aberration test, which is convenient to use, can better simplify the operation steps, and can be better used for cell chromosome aberration test, becomes a technical problem to be solved by the technical personnel in the field.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned drawbacks of the prior art, the technical problem to be solved by the present invention is,
in order to achieve the above object, the present invention provides an apparatus for in vitro mammalian cell chromosome aberration test, comprising a culture dish with a rectangular flat box structure and an open upper end; the culture dish is characterized in that a first partition plate and a second partition plate are distributed in the culture dish in a criss-cross mode so as to partition the interior of the culture dish into a plurality of culture units which are mutually independent and used for containing culture solution; the upper surfaces of the first clapboard and the second clapboard are positioned below the end surface of the open end of the culture dish; a glass slide rack is arranged in the culture dish, the glass slide rack comprises a support plate which is horizontally arranged above the first partition plate and the second partition plate, a connecting frame which is arranged downwards is arranged on each support plate and corresponds to the culture unit, a horizontal support sheet is connected to the lower end of the connecting frame, a placing station is formed above the support sheet and used for placing a glass slide, and a culture surface is formed on the upper surface of the glass slide and used for culturing mammalian cells; the support plates are respectively provided with a yielding port corresponding to the glass slide, so that the metaphase mitotic image blocker dropping liquid can pass through the yielding ports and drop onto the glass slide; the upper end of the culture dish is also provided with a horizontal cover plate.
Thus, when the device is used, culture solution is firstly added into the culture dish, then mammalian cells are added onto the glass slides, the glass slides added with the mammalian cells are horizontally placed on the supporting sheets at the lower ends of the glass slide racks in a one-to-one correspondence mode, then the glass slide racks with the glass slides are placed into the culture dish in a corresponding mode, and each glass slide is placed into the culture unit in a corresponding mode. The culture solution in each culture unit in the culture dish can contact and cover the glass slide and culture the mammalian cells, and the cover plate is covered to culture the mammalian cells in the closed space. The cover plate is then opened and the desired reagent (metaphase blocker colchicine) can be added from the relief port in the support plate. And finally, taking the whole slide glass rack out of the culture dish and entering the next step. The device can better culture the mammalian cells on the plurality of glass slides respectively, cannot interfere with each other, is more convenient to take out the glass slides together, is more convenient to operate and can save time. The device has the characteristics of convenience in use, capability of better simplifying operation steps, efficiency improvement and better application to cell chromosome aberration tests.
As optimization, the glass slide rack is also provided with a dyeing plate groove which is integrally designed into a flat box body structure and is provided with an opening at the upper end, the glass slide rack can be correspondingly arranged in the dyeing plate groove, and glass slides at the lower end of the glass slide rack can be respectively contacted with a dyeing reagent in the dyeing plate groove to complete dyeing; and a dyeing plate groove cover plate is also arranged at the upper end of the dyeing plate groove.
Thus, after the whole slide glass rack is taken out of the culture dish, the whole slide glass rack is put into the dyeing plate groove, and the dyeing reagent is contained in the dyeing plate groove, so that cells on a plurality of slide glasses can be dyed at one time. The automatic feeding device has the advantages of being capable of improving working efficiency and convenient to operate.
As optimization, the link is including connecting the connecting rod at the backup pad lower surface, and the connecting rod is four and the downward setting that are the rectangle distribution on the horizontal direction, and four connecting rods are connected respectively at four corner positions of backing sheet.
Therefore, the structural design of the connecting frame is simpler, and the culture solution in the culture unit can cover the glass slide more conveniently.
As optimization, the lower ends of the four connecting rods are respectively inclined inwards so that the four connecting rods are integrally designed into a regular quadrangular pyramid structure.
Like this, the design is more reasonable for the butt joint that slide glass frame can be better is placed in the culture dish.
For optimization, a rectangular groove which is designed to be downwards sunken is arranged in the middle of the upper surface of the support sheet, a plurality of through holes are formed in the bottom surface of the rectangular groove, and the outer contour of the glass slide is larger than that of the rectangular groove.
Thus, the structural design is more reasonable, and cell culture and staining can be better completed.
Preferably, the culture dish is made of colorless transparent acrylic materials.
Like this, the culture dish is made for colorless transparent ya keli material, and the selection of material is more reasonable, can make things convenient for the observation more.
Preferably, the first partition plate and the second partition plate are respectively two and one to divide the interior of the culture dish into eight culture units which are independent of each other.
Like this, with eight culture unit of culture dish inside branch, can once accomplish eight group's cell culture, improve work efficiency.
As optimization, the length, width and height of the culture dish are respectively 110mm, 60mm and 10 mm.
Thus, the size of the culture is more reasonably designed.
As optimization, the middle part of the cover plate protrudes downwards to form a protruding limiting part which is butted with the inner part of the upper end of the culture dish.
Like this, the design of apron is more reasonable, establishes the apron lid in culture dish upper end back, and its horizontal migration of avoiding that can be better for the lid connects more reliably, better formation enclosure space.
Drawings
FIG. 1 is a schematic cross-sectional view of a culture dish, a slide holder and a cover plate in an embodiment of the present invention.
FIG. 2 is a schematic view of the structure of the culture dish of FIG. 1 in a top view.
Fig. 3 is a partially enlarged schematic view of a position a in fig. 1.
FIG. 4 shows a slide holder and a cover plate for a trough of a dyeing plate according to an embodiment of the present invention
Schematic cross-sectional view after mating.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and examples, wherein the terms "upper", "lower", "left", "right", "inner", "outer", and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings, which is for convenience and simplicity of description, and does not indicate or imply that the referenced devices or components must be in a particular orientation, constructed and operated in a particular manner, and thus should not be construed as limiting the present invention. The terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
As shown in fig. 1 to 4, an apparatus for in vitro chromosome aberration test of mammalian cells comprises a culture dish 1 having a rectangular flat box structure and an open upper end; the culture dish is internally and crisscross distributed with a first clapboard 2 and a second clapboard 3 so as to divide the interior of the culture dish into a plurality of culture units 4 which are mutually independent and are used for containing culture solution; the upper surfaces of the first clapboard and the second clapboard are positioned below the end surface of the open end of the culture dish; a glass slide frame 5 is arranged in the culture dish, the glass slide frame comprises a support plate 6 which is horizontally arranged above the first partition plate and the second partition plate, a connecting frame 7 which is arranged downwards is arranged on the support plate respectively corresponding to the culture units, a horizontal support sheet 8 is connected to the lower end of the connecting frame, a placing station is formed above the support sheet and used for placing a glass slide 9, and a culture surface is formed on the upper surface of the glass slide and used for culturing mammalian cells; a yielding port 10 is respectively arranged on the supporting plate corresponding to the glass slides, so that the middle division image blocker dropping liquid can pass through the yielding port and drop onto the glass slides; a horizontal cover plate 11 is also provided at the upper end of the culture dish.
Thus, when the device is used, culture solution is firstly added into the culture dish, then mammalian cells are added onto the glass slides, the glass slides added with the mammalian cells are horizontally placed on the supporting sheets at the lower ends of the glass slide racks in a one-to-one correspondence mode, then the glass slide racks with the glass slides are placed into the culture dish in a corresponding mode, and each glass slide is placed into the culture unit in a corresponding mode. The culture solution in each culture unit in the culture dish can contact and cover the glass slide and culture the mammalian cells, and the cover plate is covered to culture the mammalian cells in the closed space. The cover plate is then opened and the desired reagent (metaphase blocker colchicine) can be added from the relief port in the support plate. And finally, taking the whole slide glass rack out of the culture dish and entering the next step. The device can better culture the mammalian cells on the plurality of glass slides respectively, cannot interfere with each other, is more convenient to take out the glass slides together, is more convenient to operate and can save time. The device has the characteristics of convenience in use, capability of better simplifying operation steps, efficiency improvement and better application to cell chromosome aberration tests.
In the specific embodiment, the glass slide rack further comprises a dyeing plate groove 12 which is integrally designed in a flat box structure and has an open upper end, the glass slide rack can be correspondingly arranged in the dyeing plate groove, and glass slides at the lower end of the glass slide rack can be respectively contacted with a dyeing reagent in the dyeing plate groove to complete dyeing; and a dyeing plate groove cover plate 13 is arranged at the upper end of the dyeing plate groove.
Thus, after the whole slide glass rack is taken out of the culture dish, the whole slide glass rack is put into the dyeing plate groove, and the dyeing reagent is contained in the dyeing plate groove, so that cells on a plurality of slide glasses can be dyed at one time. The automatic feeding device has the advantages of being capable of improving working efficiency and convenient to operate.
In this embodiment, the connecting frame includes connecting rod 14 connected at the bottom surface of the supporting plate, and the connecting rod is four that are distributed in a rectangle in the horizontal direction and set up downwards, and four connecting rods are connected respectively at four corner positions of supporting piece.
Therefore, the structural design of the connecting frame is simpler, and the culture solution in the culture unit can cover the glass slide more conveniently.
In this embodiment, the lower ends of the four connecting rods 14 are respectively arranged in an inward inclined manner, so that the four connecting rods are integrally designed in a regular quadrangular pyramid structure.
Like this, the design is more reasonable for the butt joint that slide glass frame can be better is placed in the culture dish.
In the present embodiment, a rectangular groove 15 with a downward concave design is formed in the middle of the upper surface of the support sheet, and a plurality of through holes 16 are formed in the bottom surface of the rectangular groove, so that the outer contour of the glass slide is larger than the contour design of the rectangular groove.
Thus, the structural design is more reasonable, and cell culture and staining can be better completed.
In this embodiment, the culture dish 1 is made of a colorless transparent acrylic material.
Like this, the culture dish is made for colorless transparent ya keli material, and the selection of material is more reasonable, can make things convenient for the observation more.
In this embodiment, the first partition plate and the second partition plate are two and one, respectively, to divide the inside of the culture dish into eight culture units independent of each other.
Like this, with eight culture unit of culture dish inside branch, can once accomplish eight group's cell culture, improve work efficiency.
In this embodiment, the length, width and height of the culture dish 1 are 110mm, 60mm and 10mm, respectively.
Thus, the size of the culture is more reasonably designed.
In this embodiment, the middle of the cover plate protrudes downward to form a protruding limiting part 17 which is butted with the inner part of the upper end of the culture dish.
Like this, the design of apron is more reasonable, establishes the apron lid in culture dish upper end back, and its horizontal migration of avoiding that can be better for the lid connects more reliably, better formation enclosure space.
Specifically, the middle part of the cover plate of the dyeing plate groove is also provided with a protruding limit part which protrudes downwards to form butt joint with the inner part of the upper end of the dyeing plate groove.
The foregoing detailed description of the preferred embodiments of the utility model has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (9)
1. A device for in vitro mammal cell chromosome aberration test comprises a culture dish with a rectangular flat box structure and an open upper end; the culture dish is characterized in that a first partition plate and a second partition plate are distributed in the culture dish in a criss-cross mode so as to partition the interior of the culture dish into a plurality of culture units which are mutually independent and used for containing culture solution; the upper surfaces of the first clapboard and the second clapboard are positioned below the end surface of the open end of the culture dish; a glass slide rack is arranged in the culture dish, the glass slide rack comprises a support plate which is horizontally arranged above the first partition plate and the second partition plate, a connecting frame which is arranged downwards is arranged on each support plate and corresponds to the culture unit, a horizontal support sheet is connected to the lower end of the connecting frame, a placing station is formed above the support sheet and used for placing a glass slide, and a culture surface is formed on the upper surface of the glass slide and used for culturing mammalian cells; the support plates are respectively provided with a yielding port corresponding to the glass slide, so that the metaphase mitotic image blocker dropping liquid can pass through the yielding ports and drop onto the glass slide; the upper end of the culture dish is also provided with a horizontal cover plate.
2. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 1, wherein: the glass slide rack is characterized by also comprising a dyeing plate groove which is integrally designed into a flat box body structure and is provided with an opening at the upper end, the glass slide rack can be correspondingly arranged in the dyeing plate groove, and glass slides at the lower end of the glass slide rack can be respectively contacted with a dyeing reagent in the dyeing plate groove to complete dyeing; and a dyeing plate groove cover plate is also arranged at the upper end of the dyeing plate groove.
3. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 1, wherein: the link is including connecting the connecting rod at the backup pad lower surface, and the connecting rod is four and the downward setting that are the rectangle distribution on the horizontal direction, and four connecting rods are connected respectively at four corner positions of backup pad.
4. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 3, wherein: and the lower ends of the four connecting rods are respectively inclined towards the inside so that the four connecting rods are integrally designed into a regular quadrangular pyramid structure.
5. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 1, wherein: the middle position of the upper surface of the supporting sheet is provided with a rectangular groove which is designed to be downwards sunken, the bottom surface of the rectangular groove is provided with a plurality of through holes, and the outline of the glass slide is larger than the outline design of the rectangular groove.
6. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 1, wherein: the culture dish is made of colorless transparent acrylic material.
7. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 1, wherein: the first partition plate and the second partition plate are respectively two and one to divide the interior of the culture dish into eight culture units which are independent of each other.
8. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 1, wherein: the length, width and height of the culture dish are 110mm, 60mm and 10mm respectively.
9. An apparatus for in vitro testing of chromosomal aberration in mammalian cells according to claim 1, wherein: the middle part of the cover plate protrudes downwards to form a protruding limit part which is butted with the inner part of the upper end of the culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122169132.5U CN215906197U (en) | 2021-09-08 | 2021-09-08 | Device for chromosome aberration test of in-vitro mammalian cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122169132.5U CN215906197U (en) | 2021-09-08 | 2021-09-08 | Device for chromosome aberration test of in-vitro mammalian cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215906197U true CN215906197U (en) | 2022-02-25 |
Family
ID=80294203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122169132.5U Active CN215906197U (en) | 2021-09-08 | 2021-09-08 | Device for chromosome aberration test of in-vitro mammalian cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215906197U (en) |
-
2021
- 2021-09-08 CN CN202122169132.5U patent/CN215906197U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4308351A (en) | System for growing tissue cultures | |
| US3745091A (en) | Biological reaction chamber apparatus | |
| US4304865A (en) | Harvesting material from micro-culture plates | |
| US5525512A (en) | Incubator | |
| CN201737950U (en) | Special experimental apparatus for cell growing on cover glass | |
| CN215906197U (en) | Device for chromosome aberration test of in-vitro mammalian cell | |
| CN103743612A (en) | Detachable glass slide incubator with adjustable volume and airtight space | |
| Minuth et al. | A new method culturing renal cells under permanent superfusion and producing a luminal-basal medium gradient | |
| Norrby et al. | A tissue model for the study of cell proliferation in vitro | |
| CN210741959U (en) | Automatic preparation facilities of chromosome sample | |
| US20240018452A1 (en) | Chip for integrated tumor cell behavior experiments | |
| CN210376190U (en) | Electron microscope dyeing sample making devices | |
| CN214251731U (en) | Chromosome dripping auxiliary device | |
| CN211401881U (en) | Batch slide staining rack | |
| CN213180970U (en) | Continuous drip box | |
| JPH01174373A (en) | Laboratory dish, laboratory dish system, cell culture apparatus using thereof and culturing method | |
| CN218297764U (en) | Chromosome karyotype analysis pretreatment workstation | |
| CN218728327U (en) | Simple nuclear type analysis dripping device | |
| CN115433672A (en) | Automatic biological material processing device, optical system and processing method thereof | |
| CN212780183U (en) | Experimental device capable of processing tissue slice samples in large batch | |
| CN218725874U (en) | Nuclear type analysis dropping piece device | |
| CN110438006A (en) | A kind of adherent unicellular point of Double layer culture device | |
| CN213447162U (en) | Multifunctional separation culture dish convenient for cell staining and observation | |
| CN201424477Y (en) | Integrated preparation box for cell and cell climbing sheet | |
| CN208155697U (en) | A kind of Oocyte in Vitro operating device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |