CN213447162U - Multifunctional separation culture dish convenient for cell staining and observation - Google Patents
Multifunctional separation culture dish convenient for cell staining and observation Download PDFInfo
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- CN213447162U CN213447162U CN202021585017.5U CN202021585017U CN213447162U CN 213447162 U CN213447162 U CN 213447162U CN 202021585017 U CN202021585017 U CN 202021585017U CN 213447162 U CN213447162 U CN 213447162U
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Abstract
The utility model relates to an in vitro cell experiment technical field, in particular to multi-functional partition culture dish convenient to cell staining and observation contains the culture dish body, division board, glass piece, has special hand-basket and culture dish lid of folding bracket. The culture dish body is separated into a first culture dish body and a second culture dish body through a partition plate, the bottom surfaces of the special lifting baskets are circular and 2 in number, and the first culture dish body and the second culture dish body are respectively placed one by one and are respectively fixed at two ends of the culture dish body and the partition plate through foldable supports. The utility model discloses a cell staining and photographing are convenient through the special basket design; after the basket is removed, the culture medium is also suitable for culturing two kinds of cells or culturing the same kind of cells under different conditions, and can also be used for preparing a solid culture medium plate and identifying and screening microorganisms such as bacteria, fungi and the like. The utility model discloses widened the application scope of culture dish, it is easy and simple to handle, reducible experimental error improves experimental efficiency.
Description
Technical Field
The utility model relates to an in vitro cell experiment technical field, in particular to multi-functional partition culture dish convenient to cell staining and observation.
Background
Cell culture dishes that are conventionally used for cell culture and that can be stained and photographed include confocal laser dishes and general cell culture dishes with built-in cell slides. The first is a laser confocal capsule, which has a thin bottom and good light transmittance, and has the specifications of 10mm, 14mm, 20mm, 30mm and the like at present. The laser confocal small dish is formed by sticking medical shadowless glue, and although the small dish plays a certain role in protecting and supporting cells on the premise of ensuring good light transmittance, the bottom of the dish is easy to fall off compared with a common cell culture dish. For the laser confocal dish, cells are easy to gather at the central groove when being spotted, so that the problem of local cell growth and accumulation occurs. The second type is a common cell culture dish with a built-in cell slide, and the cell slide has the characteristic of super-strong adsorption after advanced surface modification treatment. However, the climbing sheet is easy to break under the action of external force because the climbing sheet is low in hardness and needs to be taken out by sharp tools such as tweezers in the using process. In the case of a general cell culture dish with a built-in slide, the impact of the liquid discharged from the liquid dispenser during the liquid changing operation may damage the cells on the slide or move the slide, which may eventually lead to the falling of the cells. Meanwhile, the cell culture dish can only culture one cell at the same time, or only provide a single culture environment for the cell, and the experimental efficiency is not high.
Disclosure of Invention
The utility model provides a multi-functional culture dish of separating convenient to cell staining and observation, the design of special hand-basket and glass piece gomphosis makes the cell staining and shoot more convenient. The design of the partition plate provides the possibility of simultaneously carrying out a plurality of groups of experiments. After the basket is taken out, the method is also suitable for culturing two conventional cells or culturing the same cell under different conditions. In addition, the culture dish can also be used for preparing solid culture medium plates and identifying and screening microorganisms such as bacteria, fungi and the like. A multifunctional separation culture dish convenient for cell staining and observation comprises: the culture dish comprises a culture dish body, a culture dish cover, a partition plate, a glass sheet and a special basket, wherein the culture dish body and the culture dish cover are not communicated with each other, the partition plate is fixed at the central axis of the culture dish body and is vertical to the bottom of the culture dish, the glass sheet is embedded in holes of the basket, and the special basket is hung at the center of the culture dish body.
Preferably, the culture dish body is made of optically transparent polystyrene plastic, and the material is colorless, tasteless and nontoxic, and has the advantages of high transparency (the light transmittance of the material can reach more than 90%), good rigidity, chemical corrosion resistance and good buffering and shock resistance. The culture dishes are sterilized at high temperature and high pressure, are packaged independently in a sterile manner, and can be directly put into cell culture for use.
Preferably, the bottom end of the culture dish body and the top end of the culture dish cover are provided with corresponding numbers of 1 and 2, and the number marks are located at the edges and are transparent and convex to avoid mixing different cell samples in the first culture dish body and the second culture dish body. The culture dish body outer ring side is equipped with the little boss of arc, carries out buckle cooperation with the arc recess of culture dish lid inner ring side and is connected, has avoided the culture dish body to lead to removing dropping of in-process because of being connected not hard up with the culture dish lid.
Preferably, each of the first culture dish body and the second culture dish body is provided with a special basket with a hole. The material of hand-basket is ordinary plastics, and the hand-basket base has certain thickness, is equipped with big end down's hole in base central axis department. The holes are all round and are big in top and small in bottom. The diameter of the big hole is 20mm, the diameter of the small hole is 15mm, and the height difference between the two holes is 8 mm.
Preferably, a high-quality glass sheet with a thin bottom surface and good light transmission performance can be embedded between the two holes. The glass sheet is circular, the diameter of the glass sheet is consistent with that of the big hole in the center of the lifting basket, and the thickness of the glass sheet is the height difference of the two holes. When the glass sheet is placed in the hole, the upper surface of the glass sheet and the upper surface of the basket are positioned on the same horizontal plane.
Preferably, the two sides of the base of the special lifting basket are respectively provided with a foldable bracket which is made of common plastics. The lower end of the foldable support is provided with a rotating shaft which can rotate around a designated shaft, namely a fixed position, and the rotating angle range is 0-180 degrees. The top end of the foldable support is provided with an arc-shaped handle.
Preferably, the partition plate upper frame is provided with a wedge block, and the connection mode of the wedge block and the partition plate is a buckle design. The wedge is ordinary plastics material, and its middle part recess is connected with division board both sides, all distributes the locating hole on 1 wedge in first culture dish body and second culture dish body, the locating hole is circular, and circular locating hole plane and culture dish body top are in same horizontal plane, the hole adaptation is in the terminal handle of connecting plate. The handle is connected with the holes at the two ends of the wedge-shaped block.
The utility model discloses think about ingenious, reasonable in design passes through the division board at the bottom of the culture dish and forms first culture dish body and the second culture dish body, and two kinds of cell culture or the culture under the different conditions of homogeneous cell of being convenient for. The foldable special basket is connected in the culture dish, so that cells can be conveniently dyed and photographed, the cell damage caused by the impact force of liquid discharge of a liquid shifter in the liquid changing operation process can be avoided by the glass sheet design embedded at the bottom of the basket, the excessive accumulation problem of cell growth can be avoided, and the culture dish is suitable for long-term in vitro culture.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
The technical solution of the present invention is further elaborated below by the accompanying drawings and the concrete implementation examples.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the features and advantages of the invention, and are not intended to limit the invention. In the drawings:
FIG. 1 is a schematic structural view of a multifunctional partition culture dish for facilitating cell staining and observation according to an embodiment of the present invention;
FIG. 2 is a top view of a multi-functional partitioned culture dish for facilitating cell staining and observation in an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a single special basket in a multifunctional partition culture dish for facilitating cell staining and observation according to an embodiment of the present invention;
FIG. 4 is a partial enlarged view of a large-diameter hole and a small-diameter hole in the middle of a basket of a multifunctional partition culture dish for facilitating cell staining and observation according to an embodiment of the present invention;
FIG. 5 is a schematic view of a special basket in a multifunctional partitioned culture dish for staining and observing cells when a photo is taken after staining;
FIG. 6 is a schematic structural view of a concave wedge engaged with a partition plate in a multifunctional partition culture dish for facilitating cell staining and observation according to an embodiment of the present invention;
fig. 7 is a schematic view of the rotation range of the foldable support in the multifunctional separation culture dish for conveniently staining and observing cells according to the embodiment of the present invention.
Detailed Description
The present invention now will be described more fully hereinafter with reference to the accompanying drawings, in which embodiments of the invention are shown and described, and in which it is to be understood that the same is by way of illustration and explanation only and is not intended to be considered as limiting the scope of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
In the description of the embodiments, the following description order is merely for convenience of description and should not be construed as indicating or implying relative importance. Features mentioned in connection with different embodiments may be combined with each other as long as they do not conflict with each other.
As shown in figure 1, the utility model provides a multi-functional separated culture dish convenient to cell staining and observation, including the culture dish body (1) and culture dish lid (2), culture dish lid (2) are installed to culture dish body (1) top, and culture dish body (1) can be divided into first culture dish body (1-2) and second culture dish body (1-3). The upper parts of the outer sides of the first culture dish body (1-2) and the second culture dish body (1-3) are provided with arc-shaped small bosses (1-1), and the bosses are connected with the arc-shaped grooves (2-1) on the outer ring side of the culture dish cover (2) in a buckling manner.
The working principle and the effective achievement of the technical scheme are as follows: the partition culture dish (1) is equally divided into two parts, and the partition plate (3) at the center is integrally designed with the first culture dish body (1-2) and the second culture dish body (1-3), so that the partition culture dish can be used for culturing different samples at the same time or used for repeated experiments of the same sample, the experiment time is greatly saved, and the experiment errors are reduced. The small arc-shaped boss (1-1) on the outer ring side of the culture dish body (1) is matched and connected with the arc-shaped groove (2-1) on the inner ring side of the culture dish cover (2), so that the separation of the culture dish cover (2) and the culture dish body (1) during culture dish moving is avoided, and the risk of bacterial contamination is reduced. The bottom of the culture dish body (1) and the top of the culture dish cover (2) are provided with paired identification numbers '1' and '2', so that sample confusion caused by manual culture dish moving or misoperation can be avoided.
As shown in the figures 1, 2 and 4, the sections of the central shafts of the culture dish bodies (1) are separated by partition plates (3), special baskets (4) with holes (5) are hung in the two culture dish bodies, the centers of the bottoms of the baskets comprise large-aperture holes (5-1) above and small-aperture holes (5-2) below, and the large-aperture holes (5-1) and the small-aperture holes (5-2) are mutually embedded with circular glass sheets (8).
The working principle and the effective achievement of the technical scheme are as follows: the handle (11) arranged in the culture dish body is connected with the culture dish body (1) and the circular positioning holes (3-1 and 3-2) arranged at the two ends of the wedge-shaped block (3-3) arranged on the partition plate (3), so that the special lifting basket (4) can be hung in the culture dish body to avoid contact with the bottom of the culture dish. When the cell administration treatment operation is carried out, the special lifting basket (4) can be directly transferred to other culture media, so that the impact force on the cell surface caused by the liquid in the liquid gun in the liquid changing process or the administration treatment is avoided. When cell staining and photographing experiments are not involved, the first culture dish body (1-2) and the second culture dish body (1-3) can be used for daily cell culture or preparing solid culture medium plates and identifying and screening microorganisms such as bacteria and fungi after the special lifting basket (4) is removed. The large-aperture hole (5-1) and the glass sheet (8) are mutually embedded, and the thickness of the glass sheet is consistent with the height difference of the large and small holes, so that cells are prevented from being accumulated on two sides of the groove during the culture of a confocal dish, the situation that the number of the cells in the middle is small and the number of the cells on two sides is large is avoided, and the follow-up operation and observation are facilitated. The novel design of this experiment glass piece (8) top surface is in same horizontal plane with the inboard bottom of culture dish body (1), and the screens design of the circular glass piece (8) of bottom makes it gomphosis in special hand-basket (4) centre of a circle department, can avoid subsiding the cell growth that the maldistribution leads to and pile up, is applicable to long-term culture process. Above technical scheme can guarantee that the cell is fixed and well grows in the upper surface department of circular glass piece (8), and the dyeing and the observation of the sample of being convenient for have solved ordinary glass piece cell and have distributed inhomogeneous and lead to cell growth pile up, the film-making is difficult and the difficult scheduling problem of digging the piece before dyeing.
As shown in fig. 3, 5 and 7, in one embodiment, the bottom end of the special basket (4) is connected to the foldable support (6) through a rotating shaft (7), and the rotating shaft (7) can rotate along the folding lines (9) and (10) by 0-180 °. The top end of the foldable support (6) is provided with an arc-shaped handle (11), and the handle (11) is connected with circular positioning holes (3-1 and 3-2) on the left side and the right side of the wedge-shaped block (3-3). Foldable support (6) can be followed folding line (9, 10) and rotated 0 ~ 180, can hang the hand-basket when foldable support (6) and culture dish body (1) bottom are 90, when foldable support (6) outside side rotation to be parallel with culture dish body (1) bottom surface, then can put it on the objective table, carry out the observation of shooing after dyeing.
The working principle and the effective achievement of the technical scheme are as follows: the middle of the special lifting basket (4) is nested with a glass sheet (8), and a certain gap is reserved between the glass sheet (8) and the bottom surface. The existing laser confocal small dish in the current market has the defects that the growth distribution of cells after sedimentation is uneven due to the height difference of the center, and the middle observation area is small during photographing, so that the experimental results are greatly influenced; the laser confocal small dish is small in size, and the design of the threads on the outer ring side of the laser confocal small dish enables the culture dish to be easily overturned or culture liquid to splash when the culture dish cover is unscrewed; the bottom of the laser confocal cuvette has good light transmission performance, and if bottom scratches or pollution is caused by manual operation, the subsequent observation effect can be directly influenced. The cell climbing slice technology that has in the existing market needs to press from both sides the climbing slice with sharp-pointed instrument such as tweezers when dyeing and shooing, and at this in-process, if the power is excessive or the operation is improper easily presss from both sides garrulous climbing slice and causes the damage to the cell, influences the experimental result. The utility model relates to a do not relate to the people and touch the cultivation sample, direct touch culture dish body (1) bottom can not influence the experimental result yet. The special lifting basket (4) is connected with the foldable support (6) in the culture dish at an angle of 90 degrees, when the special lifting basket is taken out for taking a picture, the special lifting basket can be placed on an object stage, and the foldable support (6) is rotated outwards by 90 degrees until the special lifting basket is parallel to the object stage. The glass sheet (8) can not directly contact the objective table, the distance between the glass sheet (8) and the objective lens at the lower end of the objective table of the inverted microscope is further shortened, the glass sheet (8) is embedded between the large-aperture hole (5-1) and the small-aperture hole (5-2), visual field blind areas are reduced during observation, accidental factors influencing a photographing result are reduced, and the dyeing of cells and the photographing result are more accurate.
In this embodiment, the culture dish body (1) and the culture dish cover (2) are made of polystyrene subjected to super adsorption treatment.
In the embodiment, the culture dish body (1) is divided into the first culture dish body (1-2) and the second culture dish body (1-3), so that the space utilization rate of the culture dish is improved.
The working principle and the beneficial effects of the technical scheme are as follows:
as an important tool in cell culture and staining experiments, the use effect of the cell culture dish directly influences the efficiency and success rate of the scientific experiments. The design material of culture dish body and culture dish lid generally is glass or ordinary plastics, the utility model discloses a polystyrene material, its advantage is high transparency, its luminousness can reach more than 90%, has that electrical insulation is good, the easy colouration, processing mobility is good, the rigidity is good and advantage such as chemical resistance is good. Simultaneously, utilize the design of embedding glass piece (8) in the middle of division board (3) and the hand-basket, solved traditional copolymerization burnt capsule because only middle depressed part can be used to the sample culture, and the problem that the culture dish availability factor that leads to is low, occupation space is big has greatly improved the availability factor and the use variety of culture dish.
In this embodiment, the partition plate (3) and the culture dish body (1) are designed integrally.
The working principle and beneficial results of the technical scheme are as follows: the traditional culture dish with a separation design mostly adopts a structure of a detachable separation plate, the manufacture relates to materials such as glue, the existing glue material which is nontoxic to cells has high manufacturing cost and falling risk, and is not suitable for large-scale use of the separation culture dish used in experiments; in addition, although the glass material has no toxic and side effects on cell growth in long-term practice, the glass material has the defects of fragility, poor light transmittance and the like. The utility model relates to a for the integrated design, the product preparation does not relate to cohesiveness materials such as glue, has avoided the material to paste insecure and the risk that drops, and the culture dish body (1), culture dish lid (2) and glass piece (8) used material be that light transmittance is good, nontoxic and have strong adsorbability's polystyrene material, above technical scheme molding practicality is strong, the technology is brief, furthest has strengthened the system value of culture dish in the design, has enlarged the utility model discloses a practical scope to reduce the use of materials such as glue, prevented to produce the adverse effect to cultivateing the sample, improve the success rate of experiment effectively.
It is clear that the above examples are only intended to illustrate the present invention, and are not exhaustive of the embodiments. Modifications and variations of the present invention in different forms can be made by those skilled in the art based on the above description, and it is within the scope of the present invention if they are within the claims and their equivalents.
Claims (10)
1. The utility model provides a multi-functional separation culture dish convenient to cell staining and observation, includes the culture dish body (1) and locates culture dish lid (2) of its top, its characterized in that culture dish body (1) bottom is circular, and inside is provided with division board (3), division board (3) perpendicular with the bottom surface of culture dish body (1), and be the integration with culture dish body (1) and be connected, culture dish lid (2) diameter is greater than culture dish body (1), and the inner circle side is equipped with arc recess (2-1), and culture dish body (1) outer lane side is equipped with arc little boss (1-1), makes culture dish body (1) and culture dish lid (2) cooperation be connected.
2. The multifunctional separation culture dish convenient for cell staining and observation according to claim 1, wherein a special lifting basket (4) is suspended in the culture dish body (1) and is parallel to the bottom surface of the culture dish body (1), holes (5) are arranged at the center of the special lifting basket (4) and are respectively a large-aperture hole (5-1) and a small-aperture hole (5-2), the large-aperture hole (5-1) and the small-aperture hole (5-2) are located on the same central axis, and the diameters of the large-aperture hole and the small-aperture hole have a difference of 5 mm.
3. The multifunctional separation culture dish convenient for cell staining and observation according to claim 1, wherein a foldable bracket (6) fixed on the special basket (4) is installed on the base of the special basket (4), the foldable bracket (6) is connected with the base of the special basket (4) through a rotating shaft (7), the bracket (6) is hung in the culture dish body (1) along the vertical direction, and the bracket can be rotated outwards by 90 degrees to be parallel to the surface of the object stage when being photographed and observed after cell staining.
4. The multifunctional separation culture dish for facilitating cell staining and observation according to claim 1, it is characterized in that the culture dish body (1) is evenly divided into a first culture dish body (1-2) and a second culture dish body (1-3) which are semi-circular arc-shaped and are not communicated with each other through a division plate (3) in order to avoid mixing the two culture dish bodies, the bottom of the first culture dish body (1-2) is marked with a number '1', the bottom of the second culture dish body (1-3) is marked with a number '2', the partition plate (3) is vertical to the bottom surface of the culture dish body (1), the culture dish cover (2) and the culture dish body (1) are designed in a buckling mode, the culture dish cover (2) takes the top end of the partition plate (3) as a boundary, and numbers '1' and '2' are correspondingly marked on the top end surface of the culture dish cover (2).
5. The multifunctional separation culture dish convenient for cell staining and observation according to claim 1, wherein circular positioning holes (3-1, 3-2) are formed in the left side and the right side of the partition plate (3), the positioning holes (3-1, 3-2) are located on the wedge-shaped block (3-3), and the plane of the positioning holes is parallel to the plane of the top end of the culture dish body (1).
6. The multifunctional separation culture dish convenient for cell staining and observation according to claim 2, wherein the special basket (4) is circular and is provided with holes (5) with different apertures, the large aperture hole (5-1) and the small aperture hole (5-2) are both circular and are located at the central axis of the basket (4), and the connection of the holes is a concave curved surface.
7. The multifunctional separation culture dish for cell staining and observation according to claim 2, wherein a glass sheet (8) can be embedded between the large-diameter hole (5-1) and the small-diameter hole (5-2), the height difference between the large-diameter hole (5-1) and the small-diameter hole (5-2) is the thickness of the glass sheet (8), the glass sheet (8) is located above the small-diameter hole (5-2) and does not fall off, and the top surface is at the same level with the surface of the large-diameter hole (5-1).
8. The multifunctional separation culture dish convenient for cell staining and observation according to claim 3, wherein the height of the foldable support (6) is 18mm, the plane of the bottom end of the special basket (4) is L-shaped, the special basket (4) can be taken out, the foldable support can also be folded at the folding lines (9 and 10), the foldable support and the bottom surface of the special basket (4) are positioned at the same horizontal plane after being folded, cell staining and photographing are facilitated, the top end of the foldable support is connected with an arc-shaped handle (11), and the handle (11) is connected with the outer side of the culture dish body (1) and the circular positioning holes (3-1 and 3-2).
9. The multifunctional separation culture dish for cell staining and observation according to claim 7, wherein the glass sheet (8) is circular, 20mm in diameter and 8mm in thickness.
10. The multifunctional separation culture dish convenient for cell staining and observation according to claim 7, wherein the surface of the glass sheet (8) has a permanent cationic charge after super adsorption treatment, and can adsorb the sliced tissue or cells through electrostatic action, and the glass sheet is relatively tightly adhered to the glass sheet by forming a covalent bond between the glass and the sliced tissue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021585017.5U CN213447162U (en) | 2020-07-31 | 2020-07-31 | Multifunctional separation culture dish convenient for cell staining and observation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021585017.5U CN213447162U (en) | 2020-07-31 | 2020-07-31 | Multifunctional separation culture dish convenient for cell staining and observation |
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| Publication Number | Publication Date |
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| CN213447162U true CN213447162U (en) | 2021-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021585017.5U Expired - Fee Related CN213447162U (en) | 2020-07-31 | 2020-07-31 | Multifunctional separation culture dish convenient for cell staining and observation |
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| Country | Link |
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| CN (1) | CN213447162U (en) |
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- 2020-07-31 CN CN202021585017.5U patent/CN213447162U/en not_active Expired - Fee Related
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