CN221296935U - Rotary wall cell culture container with observation window - Google Patents
Rotary wall cell culture container with observation window Download PDFInfo
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- CN221296935U CN221296935U CN202323090152.9U CN202323090152U CN221296935U CN 221296935 U CN221296935 U CN 221296935U CN 202323090152 U CN202323090152 U CN 202323090152U CN 221296935 U CN221296935 U CN 221296935U
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- container
- observation window
- cover
- cell culture
- upper cover
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- 238000004113 cell culture Methods 0.000 title claims abstract description 24
- 239000011521 glass Substances 0.000 claims abstract description 29
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 238000010276 construction Methods 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 abstract description 7
- 239000005357 flat glass Substances 0.000 abstract description 6
- 238000002834 transmittance Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 2
- 238000012604 3D cell culture Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005486 microgravity Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application relates to the technical field of cell culture and discloses a rotary wall cell culture container with an observation window, which comprises a container upper cover, a container lower cover and a container wall, wherein the container wall is fixedly arranged between the container upper cover and the container lower cover, a planar glass observation window is fixedly arranged on the surface of the container upper cover, two luer one-way valves are symmetrically and fixedly communicated with the surface of the container upper cover, a hollowed-out structure is arranged on the container lower cover, and a disposable gas exchange membrane is arranged on the front surface corresponding to the hollowed-out structure. According to the application, the plane glass observation window is colorless high turbine surface glass, the light transmittance is better, the surface of the plane glass observation window is finely ground into the observation window glass with certain flatness, the optical distortion is extremely small, the optical observation performance is better, the whole container has gas exchange capacity, the disposable gas exchange membrane can be replaced, the quick replacement can be realized when the damage occurs, and the use cost is saved.
Description
Technical Field
The application belongs to the technical field of cell culture, and particularly relates to a rotary wall cell culture container with an observation window.
Background
Cell culture refers to a method of simulating in vitro an in vivo environment (sterile, suitable temperature, ph, certain nutritional conditions, etc.) to survive, grow, reproduce and maintain major structures and functions.
The rotating wall cell culture device is used in dynamic cell culture, 3D cell culture or microgravity effect simulation culture, but the existing rotating wall cell culture device has no special observation window, is directly observed through the container wall, is a cylindrical structure because the container wall is made of transparent materials, is almost completely distorted because of the curved surface when the container wall is made, and has large optical distortion, the observation result is almost completely distorted, and the direct observation cannot be performed because of obvious optical distortion and distortion, and the rotating wall cell culture device is additionally provided with a gas exchange membrane for use, but the gas exchange membrane of the existing product cannot be replaced, but is extremely easy to cause the damage of the gas exchange membrane in the daily operation and cleaning process, and once the damage cannot be repaired, the use cost is high.
Disclosure of utility model
The present utility model is directed to a rotary wall cell culture vessel with an observation window, for solving the above-mentioned problems.
In order to achieve the above purpose, the present application adopts the following technical scheme:
The utility model provides a take rotatory wall cell culture container of observation window, includes container upper cover, container lower cover and container wall, the container wall is fixed to be installed between container upper cover and container lower cover, the fixed surface of container upper cover installs the plane glass observation window, the fixed intercommunication of surface of container upper cover still symmetry has two luer one-way valves, be equipped with hollow out construction under the container, and correspond hollow out construction's front and install disposable gas exchange membrane.
Preferably, a charging port cover is also fixedly arranged at the upper end of the upper cover of the container.
Preferably, the disposable gas exchange membrane is detachably assembled with the container lower cover.
Preferably, the container lower cover is also integrally connected with a connecting port.
Preferably, the plane glass observation window is specifically colorless high turbine face glass.
Preferably, a plurality of connecting studs are fixedly connected between the upper container cover and the lower container cover.
Compared with the prior art, the application provides the rotary wall cell culture container with the observation window, which has the following beneficial effects:
The rotary wall cell culture container with the observation window is characterized in that a container upper cover, a container lower cover, a container wall, a planar glass observation window, a luer single-way valve, a hollow structure, a disposable gas exchange membrane, a charging port cover and a connecting port are arranged, culture fluid, cells and the like are added into the culture container through the charging port cover on the container upper cover, residual air in the container is discharged through the two luer single-way valves on the container upper cover by means of a disposable syringe, then the connecting port on the container lower cover is connected to a matched host machine, after culture is finished, the container can be directly placed under a microscope for observation, the planar glass observation window on the container upper cover is used for microscopic observation during observation, the planar glass observation window is colorless high-turbine-surface glass, the planar glass observation window surface is made into observation window glass with certain flatness, optical distortion is very small, researchers can observe the true state of a sample better, the hollow structure is arranged on the container lower cover, the disposable gas exchange membrane is arranged on the container, the surface of the container can be replaced, the container can be better replaced, the gas exchange performance can be better compared with the conventional culture container, the current optical exchange membrane can be guaranteed, the culture container can be replaced with the conventional gas exchange membrane, and the container can be replaced with the conventional culture container, and the gas exchange can be rapidly replaced, and the gas exchange container can be guaranteed.
Drawings
FIG. 1 is a schematic top view of a rotary wall cell culture vessel with an observation window according to the present application;
Fig. 2 is a schematic bottom perspective view of a rotary wall cell culture vessel with an observation window according to the present application.
In the figure: 1. a container upper cover; 2. a container lower cover; 3. a vessel wall; 4. a planar glass observation window; 5. a luer single pass valve; 6. a hollow structure; 7. a disposable gas exchange membrane; 8. a charging port cover; 9. a connection port; 10. and connecting the studs.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments.
Referring to fig. 1-2, a rotary wall cell culture container with an observation window comprises a container upper cover 1, a container lower cover 2 and a container wall 3, wherein the container wall 3 is fixedly arranged between the container upper cover 1 and the container lower cover 2, a plane glass observation window 4 is fixedly arranged on the surface of the container upper cover 1, two luer one-way valves 5 are symmetrically and fixedly communicated with the surface of the container upper cover 1, a hollowed-out structure 6 is arranged on the container lower cover 2, and a disposable gas exchange membrane 7 is arranged on the front surface of the corresponding hollowed-out structure 6.
The upper end of the upper container cover 1 is also fixedly provided with a charging port cover 8.
The disposable gas exchange membrane 7 is detachably assembled with the container lower cover 2.
The container lower cover 2 is also integrally connected with a connecting port 9.
The plane glass observation window 4 is specifically colorless high turbine face glass.
A plurality of connecting studs 10 are fixedly connected between the container upper cover 1 and the container lower cover 2.
The principle of operation of the present utility model will now be described as follows:
When the culture medium container is used, through the arranged container upper cover 1, the container lower cover 2, the container wall 3, the plane glass observation window 4, the luer single-way valve 5, the hollow structure 6, the disposable gas exchange membrane 7, the charging port cover 8 and the connecting port 9, the culture medium and cells and the like are added into the culture container through the charging port cover 8 on the container upper cover 1, the residual air in the container is discharged through the two luer single-way valves 5 on the container upper cover 1 by means of the disposable injector, then the connecting port 9 on the container lower cover 2 is connected to a matched host machine, after the culture is finished, the container can be directly placed under a microscope for observation, during observation, the plane glass observation window 4 is made of colorless high-turbine surface glass, the surface of the plane glass observation window 4 is finely ground into the observation window glass with certain flatness, optical distortion is very small, a true state of a sample can be better helped by researchers, after the culture is finished, the hollow structure 6 is arranged on the container lower cover 2, the surface of the container can be directly placed under a microscope for observation, the plane glass observation window 4 is made of the plane glass observation window 4, the plane glass observation window glass is made of colorless high turbine surface glass, the plane glass is better, the surface glass is better, the observation window glass is better than the observation window glass is capable of changing the surface of the gas exchange membrane, and the gas exchange membrane can be better, compared with the conventional culture medium, and the culture medium can be replaced with the culture medium, and can be replaced with the gas exchange membrane can be quickly when the culture medium exchange can be replaced, and when the gas exchange membrane is in the like, and when the culture medium can be in the like, and can be replaced, and when compared with the gas exchange can and has the gas exchange membrane can and has the gas exchange with the gas exchange, and has the gas exchange with and can exchange.
The foregoing is only a preferred embodiment of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art, who is within the scope of the present application, shall cover the scope of the present application by equivalent substitution or modification according to the technical scheme of the present application and the application concept thereof.
Claims (6)
1. The utility model provides a take rotatory wall cell culture container of observation window, includes container upper cover (1), container lower cover (2) and container wall (3), its characterized in that, container wall (3) are fixed to be installed between container upper cover (1) and container lower cover (2), the fixed surface mounting of container upper cover (1) is equipped with plane glass observation window (4), the fixed intercommunication of surface of container upper cover (1) still symmetry has two luer single-pass valve (5), be equipped with hollow out construction (6) on container lower cover (2), and correspond disposable gas exchange membrane (7) are installed in the front of hollow out construction (6).
2. The rotary wall cell culture vessel with the observation window according to claim 1, wherein a charging port cover (8) is fixedly arranged at the upper end of the upper vessel cover (1).
3. A rotary wall cell culture vessel with a viewing window according to claim 1, characterized in that the disposable gas exchange membrane (7) is detachably assembled with the vessel lower cover (2).
4. The rotary wall cell culture vessel with the observation window according to claim 1, wherein the vessel lower cover (2) is also integrally connected with a connecting port (9).
5. A rotating wall cell culture vessel with a viewing window according to claim 1, characterized in that the planar glass viewing window (4) is in particular a colorless high turbine face glass.
6. The rotary wall cell culture vessel with the observation window according to claim 1, wherein a plurality of connecting studs (10) are fixedly connected between the upper vessel cover (1) and the lower vessel cover (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323090152.9U CN221296935U (en) | 2023-11-16 | 2023-11-16 | Rotary wall cell culture container with observation window |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323090152.9U CN221296935U (en) | 2023-11-16 | 2023-11-16 | Rotary wall cell culture container with observation window |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221296935U true CN221296935U (en) | 2024-07-09 |
Family
ID=91736319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202323090152.9U Active CN221296935U (en) | 2023-11-16 | 2023-11-16 | Rotary wall cell culture container with observation window |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221296935U (en) |
-
2023
- 2023-11-16 CN CN202323090152.9U patent/CN221296935U/en active Active
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