CN203546036U - Assembled culture dish for living cell microscopic observation - Google Patents
Assembled culture dish for living cell microscopic observation Download PDFInfo
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- CN203546036U CN203546036U CN201320737870.8U CN201320737870U CN203546036U CN 203546036 U CN203546036 U CN 203546036U CN 201320737870 U CN201320737870 U CN 201320737870U CN 203546036 U CN203546036 U CN 203546036U
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Abstract
The utility model provides an assembled culture dish for living cell microscopic observation. The assembled culture dish is composed of two parts: an outer dish and an inner dish. The assembled culture dish is characterized in that the outer dish and the inner dish can be screwed in each other through the matching threads formed on the outer dish and the inner dish so that the two bottom surfaces of the outer dish and the inner dish are in seamless close contact with each other, and a through hole is formed in the central position of the bottom of each of the inner dish and the outer dish. The assembled culture dish for living cell microscopic observation has the advantages that a plurality of circular or square cover slips can be replaced and clamped through the bottoms of the inner dish and the outer dish so that a plurality of cell samples can be detected on few culture dishes, and therefore, the detection cost is greatly reduced and the detection efficiency is improved, and the assembled culture dish is convenient for microscopic detection of living cells.
Description
The utility model relates to a kind of Novel cell culture dish, specifically, is a kind of assembly type viable cell microscopic culture dish for experimental study and medical context of detection.
Cell cultures refers to from in-vivo tissue takes out cell in vitro simulated in vivo environment, makes its growth and breeding, and maintains a kind of culture technique of its structure and function.The culture of cell cultures can be individual cells, can be also cell mass.Conventional cell cultures vessel have culturing bottle, culture plate, culture dish etc.Culture dish is a kind of labware for cell cultures, the end and a lid of a flat circle plate-like, consists of, and generally with glass or plastics, makes.For open cultivation and other purposes.Divide diameter 30mm, 60mm, 120mm etc. several.
At present, biomicroscope is inverted microscope mostly, and at viable cell, doing special detection need to be when the microscope lens,oil immersion, and camera lens will remove to see clearly the viable cell on culture dish through culture dish bottom from lower.The thickness of general Tissue Culture Dish bottom is thick, can not meet lens,oil immersion focal length and be less than the requirement below 0.5mm, therefore conventional Tissue Culture Dish can not be for the microscopical observation of this viable cell.
Therefore, need a kind of viable cell microscopic culture dish for experimental study and medical context of detection.
It is a kind of simple in structure that the purpose of this utility model is to provide, and testing cost is low, the viable cell microscopic culture dish for experimental study and medical context of detection that detection efficiency is high.
In order to address the above problem, the technical solution of the utility model is: a kind of assembly type viable cell microscopic culture dish, by outer ware and interior ware two portions, formed, it is characterized in that: outer ware and interior ware be by the matching thread of both processing, can precession and make the smooth seamlessly contact in two bottom surfaces tight; The center position of ware bottom, is processed with an outer ware through hole outside; Center position in interior ware bottom, is processed with an interior ware through hole.
In technique scheme, the diameter of phi of described interior ware through hole
in>=10mm; Described outer ware through-hole diameter Φ
outwardbe greater than the diameter of phi of interior ware through hole
inat least more than 4mm, facilitate like this below the through interior ware of microscopical camera lens, whole cells of ware through hole in guaranteeing, microlens all can clearly be observed.
In technique scheme, the means processing such as the lower surface of the lower surface of described outer ware inwall and interior ware outer wall requires to adopt finish turning, refines, finish reams, grinding, surface roughness value is less than below Ra1.6, reach and cannot see cutter trade, micro-machine direction of debating, such as surface roughness Ra 0.8, Ra0.4 etc.To guarantee two sides, the bottom no-float of interior ware and outer ware, screw rear no leakage body.
The utility model technical characterstic is that culture dish can break, and the place, bottom surface at interior ware and outer ware, can clamp diameter and be greater than outer ware through hole Φ
outwardcircular lid slide, or gripper edge is grown up in outer ware through hole Φ
outwardsquare cover glass.
Compared with prior art, the utlity model has following beneficial effect:
Available common culture dish on cover glass, in microscopic examination process, is clamped to cell cultures on the utility model by cover glass, realization detects a plurality of cell samples on a small amount of culture dish, greatly reduce testing cost, improve detection efficiency, facilitated the microscopic examination of viable cell.
Fig. 1 is sectional structure schematic diagram of the present utility model;
Fig. 2 is the schematic perspective view of transparent material of the present utility model;
In figure: 1. outer ware; 2. matching thread; 3. interior ware; 4. interior ware through hole; 5. outer ware through hole; 6. cover glass.
Below in conjunction with drawings and Examples, further the utility model is illustrated.
Referring to Fig. 1 to Fig. 2: assembly type viable cell microscopic culture dish, by outer ware 1 and interior ware 3 two portions, formed, by matching thread 2, be screwed in together.The diameter of phi of the interior ware through hole 4 of interior ware 3
in=10mm; The diameter of phi of the outer ware through hole 5 of outer ware 1
outward=14mm.
During use, first outer ware 1 and interior ware 3 are outwarded winding separated, the cover glass 6 of diameter of phi=18mm circle of cultured cell, cell is placed on the middle, bottom of outer ware 1 upward, precession also screws interior ware 3, then inwardly in ware, adds cell culture fluid, builds culture dish lid.Finally the culture dish assembling is taken to microscope place, carry out viable cell detection.
After having detected, siphon away nutrient solution, ware 3 in screwing out, changes cover glass 6, is reentered into cover glass to be detected 6, by aforementioned operation method, detects the viable cell sample on second cover glass 6.
Referring to Fig. 1 to Fig. 2: assembly type viable cell microscopic culture dish, by outer ware 1 and interior ware 3 two portions, formed, by matching thread 2, be screwed in together.The diameter of phi of the interior ware through hole 4 of interior ware 3
in=12mm; The diameter of phi of the outer ware through hole 5 of outer ware 1
outward=18mm.
During use, first outer ware 1 and interior ware 3 are outwarded winding separated, the square length of side of cultured cell is equaled to 20 * 20mm cover glass 6, cell is placed on the middle, bottom of outer ware 1 upward, precession also screws interior ware 3, then inwardly in ware 3, adds cell culture fluid, builds culture dish lid.Finally the culture dish assembling is taken to microscope place, carry out viable cell detection.
After having detected, siphon away nutrient solution, ware 3 in screwing out, changes cover glass 6, is reentered into cover glass to be detected 6, by aforementioned operation method, detects the viable cell sample on second cover glass 6.
Special instruction: the material of utility model is not limited by this specification sheets exemplary plot, and every principle of design and the different Tissue Culture Dish of same or analogous from this patent, the used material of each element, all in the protection domain of this patent.
Claims (3)
1. an assembly type viable cell microscopic culture dish, is comprised of outer ware (1) and interior ware (3) two portions, it is characterized in that: outer ware (1) is connected by both matching threads (2) of processing with interior ware (3); The center position of ware (1) bottom, is processed with an outer ware through hole (5) outside; Center position in interior ware (3) bottom, is processed with an interior ware through hole (4).
2. assembly type viable cell microscopic culture dish according to claim 1, is characterized in that: the diameter of phi of described interior ware through hole (4)
in>=10mm; The diameter of phi of described outer ware through hole (5)
outwardbe greater than the diameter of phi of interior ware through hole (4)
inat least more than 4mm.
3. assembly type viable cell microscopic culture dish according to claim 1, is characterized in that: the lower surface of the lower surface of described outer ware (1) inwall and interior ware (3) outer wall requires surface roughness value to be less than below Ra1.6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320737870.8U CN203546036U (en) | 2013-11-21 | 2013-11-21 | Assembled culture dish for living cell microscopic observation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320737870.8U CN203546036U (en) | 2013-11-21 | 2013-11-21 | Assembled culture dish for living cell microscopic observation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203546036U true CN203546036U (en) | 2014-04-16 |
Family
ID=50464181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320737870.8U Expired - Fee Related CN203546036U (en) | 2013-11-21 | 2013-11-21 | Assembled culture dish for living cell microscopic observation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203546036U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113655059A (en) * | 2021-09-02 | 2021-11-16 | 中国科学院长春应用化学研究所 | Sample liquid phase imaging device |
-
2013
- 2013-11-21 CN CN201320737870.8U patent/CN203546036U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113655059A (en) * | 2021-09-02 | 2021-11-16 | 中国科学院长春应用化学研究所 | Sample liquid phase imaging device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140416 Termination date: 20141121 |
|
| EXPY | Termination of patent right or utility model |