CN103589639A - Mobile type cell culture dish with glass bottom - Google Patents
Mobile type cell culture dish with glass bottom Download PDFInfo
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- CN103589639A CN103589639A CN201310587828.7A CN201310587828A CN103589639A CN 103589639 A CN103589639 A CN 103589639A CN 201310587828 A CN201310587828 A CN 201310587828A CN 103589639 A CN103589639 A CN 103589639A
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- culture dish
- locating slot
- ware body
- concave surface
- knob
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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Abstract
The invention provides a mobile type cell culture dish with a glass bottom. The mobile type cell culture dish consists of a dish body and elastic ropes and is characterized in that a through hole is formed in the center of the bottom of the dish body; four tight elastic ropes which form #-shaped distribution on the upper surface and the lower surface of the culture dish are arranged on the dish body in a sleeving manner and are fixed in upper positioning slot knobs and lower positioning slot knobs. According to the mobile type cell culture dish with the glass bottom, cells can be cultured on a glass slide through a common culture dish, and the glass slide is fixed on the mobile type cell culture dish with a glass bottom through the elastic ropes in a microscope detection process, so that a plurality of cell samples can be detected on a small quantity of culture dishes.
Description
The present invention relates to a kind of Novel cell culture dish, specifically, is a kind of movable glass bottom Tissue Culture Dish for experimental study and medical context of detection.
Culture dish is a kind of labware for cell cultures, the end and a lid of a flat circle plate-like, consists of, and generally with glass or plastics, makes.For purposes such as open culturing cell and other detections.
Current biomicroscope is inverted microscope mostly, with culture dish, is observing viable cell, and while particularly doing special detection with microscope lens,oil immersion, camera lens need to, from culture dish bottom through the bottom of culture dish, be seen the viable cell on culture dish clearly.The thickness of general Tissue Culture Dish bottom is thick, can not meet the requirement of lens,oil immersion focal length, so general Tissue Culture Dish can not be for the microscopical observation of this viable cell.
Therefore, need a kind of new-type viable cell microscopic culture dish for experimental study and medical context of detection.
The object of the present invention is to provide a kind of simple in structurely, testing cost is low, the viable cell microscopic culture dish for experimental study and medical context of detection that detection efficiency is high.
In order to address the above problem, technical scheme of the present invention is: a kind of movable glass bottom Tissue Culture Dish, by ware body and stretch cord two portions, formed, it is characterized in that: the center position in the bottom of ware body, be processed with a bottom through-hole, bottom through-hole around to bottom surface, be processed with the bottom concave surface of smooth square pattern.The size dimension of concave surface can, according to the size of the size of culture dish used and cover glass, be determined flexibly; Top sides edge at the ware body of culture dish, also can process on the cap top edge of culture dish, by even circumferential, is distributed with 4 upper locating slot knobs, and on the bottom face sidewall of ware body, upper and lower corresponding with the position of upper locating slot knob have 4 lower locating slot knobs; On ware body, cover has 4 stretch cords that tighten, be the distribution of " well " font at the upper and lower surface of culture dish; Stretch cord is fixed in locating slot knob and lower locating slot knob.
In technique scheme, the direction of described upper its notch of locating slot knob is consistent with stretch cord " well " font trend; The direction of lower its notch of the locating slot knob trend upper and lower with stretch cord is consistent; Lower locating slot knob in the position of ware body lower sides a little more than bottom surface, not concordant with bottom surface.The stretch cord of tightening with assurance can be fixed by upper locating slot knob and lower locating slot knob, and is close to the bottom surface of ware body.
In technique scheme, the degree of depth >=0.5mm of described bottom concave surface, and have width >=5mm, thickness to be no more than a circle waterproof sealing cushion of the bottom concave surface degree of depth in its surrounding, and gasket requires water resistance good, and toughness is better.
In technique scheme, the pattern of described bottom concave surface can be also smooth circular pattern.
The technology of the present invention feature is at the bottom of culture dish is used for observing the ware of viable cell, is greater than the circular lid slide of bottom through-hole with diameter, or the square cover glass that the length of side is greater than bottom through-hole substitutes.
Compared with prior art, the present invention has following beneficial effect:
More more flexible than general culture dish, greatly reduce testing cost, improve detection efficiency, facilitated the microscopic examination of viable cell.The present invention can with common culture dish by cell cultures on cover glass, in microscopic examination process, cover glass is fixed in the present invention with stretch cord, realize on a small amount of culture dish and detecting a plurality of cell samples.
Fig. 1 is that master of the present invention looks A-A cross-sectional view;
Fig. 2 is the schematic top plan view of transparent material of the present invention;
In figure: 1. ware body; 2. stretch cord; 3. go up locating slot knob; 4. descend locating slot knob; 5. bottom through-hole; 6. cover glass; 7. bottom concave surface; 8. waterproof sealing cushion.
Below in conjunction with drawings and Examples, further the present invention is illustrated.
Referring to Fig. 1 to Fig. 2: a kind of movable glass bottom Tissue Culture Dish, by ware body 1 and stretch cord 2 two portions, formed, it is characterized in that: the center position in the bottom of ware body 1, be processed with a bottom through-hole 5, diameter of phi=the 8mm in hole, bottom through-hole 5 is downward bottom surface around, is processed with the bottom concave surface 7 of the smooth square pattern of 25 * 25 mm, the degree of depth 0.5 mm.
During use, first the cover glass 6 of diameter of phi=18mm circle of cultured cell, cell is placed in the middle of bottom concave surface 7 upward, and guarantee to have at least the sealed elastic pad 8 of 2mm width to be attached on cover glass 6, add again a sealed elastic pad 8 that thickness is moderate, make the sealed elastic pad 8 adding slightly exceed bottom concave surface 7.Then on ware body 1, put 4 stretch cords 2 that tighten, be the distribution of " well " font at the upper and lower surface of culture dish; Stretch cord 2 is fixed in locating slot knob 3 and lower locating slot knob 4, by cover glass 6 tensions, makes it to be close on the sealed elastic pad 8 of bottom concave surface 7.In ware body 1, add cell culture fluid, build culture dish lid.Finally the culture dish assembling is taken to microscope place, carry out viable cell detection.
After having detected, siphon away nutrient solution, change cover glass 6, be reentered into cover glass to be detected 6, by aforementioned operation method, detect the viable cell sample on second cover glass 6.
Referring to Fig. 1 to Fig. 2: a kind of movable glass bottom Tissue Culture Dish, by ware body 1 and stretch cord 2 two portions, formed, it is characterized in that: the center position in the bottom of ware body 1, be processed with a bottom through-hole 5, diameter of phi=the 10mm in hole, bottom through-hole 5 is downward bottom surface around, is processed with the bottom concave surface 7 of smooth Φ=35mm circular pattern, the degree of depth 0.6 mm.
During use, first the square cover glass 6 of 22 * 22 mm of cultured cell, cell is placed in the middle of bottom concave surface 7 upward, and guarantee to have at least the sealed elastic pad 8 of 2mm width to be attached on cover glass 6, at cover glass, add again a sealed elastic pad 8 that thickness is moderate for 6 times, make the sealed elastic pad 8 adding slightly exceed bottom concave surface 7.Then on ware body 1, put 4 stretch cords 2 that tighten, be the distribution of " well " font at the upper and lower surface of culture dish; Stretch cord 2 is fixed in locating slot knob 3 and lower locating slot knob 4, by cover glass 6 tensions, makes it to be close on the sealed elastic pad 8 of bottom concave surface 7.In ware body 1, add cell culture fluid, build culture dish lid.Finally the culture dish assembling is taken to microscope place, carry out viable cell detection.
After having detected, siphon away nutrient solution, change cover glass 6, be reentered into cover glass to be detected 6, by aforementioned operation method, detect the viable cell sample on second cover glass 6.
Special instruction: the material of invention is not limited by this specification sheets exemplary plot, and every principle of design and the different Tissue Culture Dish of same or analogous from this patent, the used material of each element, all in the protection domain of this patent.
Claims (4)
1. a movable glass bottom Tissue Culture Dish, by ware body (1) and stretch cord (2) two portions, formed, it is characterized in that: the center position in the bottom of ware body (1), be processed with a bottom through-hole (5), bottom through-hole (5) is downward bottom surface around, is processed with the bottom concave surface (7) of smooth square pattern; On the top sides edge of ware body (1), by even circumferential, be distributed with 4 upper locating slot knobs (3), on the bottom face sidewall of ware body (1), upper and lower corresponding with the position of upper locating slot knob (3) have 4 lower locating slot knobs (4); At the upper cover of ware body (1), there are 4 stretch cords (2) that tighten, be the distribution of " well " font at the upper and lower surface of culture dish; Stretch cord (2) is fixed in locating slot knob (3) and lower locating slot knob (4).
2. a kind of movable glass bottom Tissue Culture Dish according to claim 1, is characterized in that: the direction of its notch of described upper locating slot knob (3) is consistent with stretch cord (2) " well " font trend; The direction of its notch of lower locating slot knob (4) trend upper and lower with stretch cord (2) is consistent; Lower locating slot knob (4) in the position of ware body (1) lower sides a little more than bottom surface, not concordant with bottom surface.
3. a kind of movable glass bottom Tissue Culture Dish according to claim 1, it is characterized in that: the degree of depth >=0.5mm of described bottom concave surface (7), and there are width >=5mm, thickness to be no more than the sealed elastic pad (8) of a circle waterproof of bottom concave surface (7) degree of depth in its surrounding.
4. a kind of movable glass bottom Tissue Culture Dish according to claim 1, is characterized in that: the pattern of described bottom concave surface (7) can be also smooth circular pattern.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310587828.7A CN103589639A (en) | 2013-11-21 | 2013-11-21 | Mobile type cell culture dish with glass bottom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310587828.7A CN103589639A (en) | 2013-11-21 | 2013-11-21 | Mobile type cell culture dish with glass bottom |
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| Publication Number | Publication Date |
|---|---|
| CN103589639A true CN103589639A (en) | 2014-02-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310587828.7A Pending CN103589639A (en) | 2013-11-21 | 2013-11-21 | Mobile type cell culture dish with glass bottom |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804551A (en) * | 2019-11-25 | 2020-02-18 | 安徽科门生物科技有限公司 | Laboratory is with lift type cell centrifuge |
| CN113848103A (en) * | 2021-10-22 | 2021-12-28 | 深圳先进技术研究院 | Microscopical appearance device and cell phenotype controlling means |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201378217Y (en) * | 2009-03-13 | 2010-01-06 | 周晋 | Observation dish for easy wall-adhering laser scanning confocal microscope |
| CN201695031U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Partitioned culture vessel with glass bottom |
| JP2011244691A (en) * | 2010-05-21 | 2011-12-08 | Asahi Glass Co Ltd | Culture container |
| CN202322860U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | High-resolution imaged cell culture dish with glass bottom |
| CN102943036A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | Glass bottom cell culture dish structure |
-
2013
- 2013-11-21 CN CN201310587828.7A patent/CN103589639A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201378217Y (en) * | 2009-03-13 | 2010-01-06 | 周晋 | Observation dish for easy wall-adhering laser scanning confocal microscope |
| CN201695031U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Partitioned culture vessel with glass bottom |
| JP2011244691A (en) * | 2010-05-21 | 2011-12-08 | Asahi Glass Co Ltd | Culture container |
| CN202322860U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | High-resolution imaged cell culture dish with glass bottom |
| CN102943036A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | Glass bottom cell culture dish structure |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804551A (en) * | 2019-11-25 | 2020-02-18 | 安徽科门生物科技有限公司 | Laboratory is with lift type cell centrifuge |
| CN113848103A (en) * | 2021-10-22 | 2021-12-28 | 深圳先进技术研究院 | Microscopical appearance device and cell phenotype controlling means |
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Application publication date: 20140219 |