CN209784649U - Sample carrying device for microscope - Google Patents
Sample carrying device for microscope Download PDFInfo
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- CN209784649U CN209784649U CN201920744720.7U CN201920744720U CN209784649U CN 209784649 U CN209784649 U CN 209784649U CN 201920744720 U CN201920744720 U CN 201920744720U CN 209784649 U CN209784649 U CN 209784649U
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Abstract
A sample loading device for a microscope, comprising: the glass cover comprises an upper plate, a gasket, a cover glass and a lower plate, wherein one or more through holes are formed in the upper plate; the gasket is fixed on the lower surface of the upper plate and surrounds the through hole in the upper plate, the lower plate is connected with the upper plate through a hinge and is provided with one or more through holes, and the shape, size and position of the through hole in the lower plate are consistent with those of the through hole in the upper plate; the cover glass is placed on the through hole of the lower plate and used for bearing a sample. The device can be used for microscopic observation and dyeing sample preparation. The device can be used repeatedly, and has low cost and wide application.
Description
Technical Field
The utility model relates to a carry appearance device suitable for microscope is particularly useful for the observation of high power objective.
Background
The current sample carrier for microscope observation is culture dish (figure 1), orifice plate, culture bottle, slide glass, etc., but these devices can not be used for high power objective observation because the bottom is thicker and larger than the working distance of the high power objective. There are two methods for observing with a high power objective: one method is to add a cover glass into a cell culture solution, to spread cells on the cover glass, then to fish up the cover glass for subsequent fluorescent staining and the like, and finally to contact the cover glass with an objective lens for microscopic observation. But the cover glass is tightly attached to the bottom of the dish and is difficult to salvage, and the cover glass is easy to be reversed during subsequent sample preparation operation, so that the fluorescent dye is added on the wrong surface, and the dyeing failure is caused.
Another method is to use a glass-based dish (FIG. 2) with a circular hole in the middle and a cover slip on the underside. Cells were cultured in glass-bottom dishes, sampled and finally directly observed with a microscope. But the range of observation is limited within this circular hole. And the glass substrate is a disposable material, can not be reused and has high cost.
Both of the loading devices of fig. 1 and 2 have side walls with a height of about 1cm, which severely limits the tilting angle and the range of motion of the micromanipulation needle during micromanipulation.
SUMMERY OF THE UTILITY MODEL
In order to solve the defects of big thickness, small observation range, high cost and the like at the bottom of the sample loading device in the prior art, the utility model provides a sample loading device for a microscope.
The utility model provides a sample carrying device for a microscope, which comprises an upper plate, a gasket, a cover glass and a lower plate, wherein one or more through holes are arranged on the upper plate; the gasket is fixed on the lower surface of the upper plate and surrounds the through hole on the upper plate; the lower plate is connected with the upper plate through a hinge and is provided with one or more through holes, and the shape, size and position of the through hole on the lower plate are consistent with those of the through hole on the upper plate; the cover glass is placed on the through hole of the lower plate and used for bearing a sample.
Preferably, the through holes of the upper plate and the through holes of the lower plate have a circular, rectangular or square shape.
Preferably, the upper plate and the lower plate are made of plastic, resin, acrylic, silicone, rubber, ABS, nylon, stainless steel or aluminum alloy.
Preferably, the through hole of the upper plate is provided with scales or numbers around.
Preferably, the gasket is made of a flexible material selected from silicone or rubber.
Preferably, the sample carrier further comprises a gel grid having one or more through holes, the gel grid being affixed to the cover glass for forming one or more chambers on the cover glass.
Preferably, the shape of the gel lattice is square, rectangular or circular.
Preferably, the rubber grid is made of a flexible material selected from silicone or rubber.
Preferably, a buckle is arranged on the lower plate, and when the upper plate and the lower plate are in a fit state, the buckle can clamp the upper plate.
Preferably, a buckle is arranged on the upper plate, and when the upper plate and the lower plate are in a fit state, the buckle can clamp the lower plate.
Compared with the prior art, the utility model discloses a carry appearance device has following beneficial effect:
1. The utility model discloses the material (including upper plate, hypoplastron, packing ring, gluey check) that the device used can use repeatedly after the disinfection, and only the coverslip is the consumer, has practiced thrift the material, compares greatly reduced cost with disposable glass end dish.
2. The utility model discloses the device is at the cover glass year appearance, can select the cover glass that thickness is less than 0.17mm, so available high power objective observes, like 100 times oil glasses. The size of the upper plate, the lower plate and the gasket of the sample carrier can be slightly larger than that of the cover glass, so that the visual field range of the whole cover glass can be observed even if the high-power objective lens is used for observation.
3. Can be used for cell culture. The utility model discloses because the leakproofness is better, can directly be used for cultivateing the cell. The utility model discloses 1cm can be designed into to the height of device, and is highly unanimous with ordinary culture dish, and the cell can be on loading and the cover glass, covers with cell culture solution, arranges the incubator in and cultivates. Meanwhile, the glue lattice divides one cover glass into a plurality of small chambers, a plurality of samples can be loaded or the same sample can be subjected to a plurality of treatments, the incubation conditions are consistent, and single-factor variable analysis is convenient to perform. A plurality of samples are loaded on one cover glass, so that the time for changing samples and focusing during observation is greatly saved.
4. Can be used for fluorescent staining. The cell is in the utility model discloses can directly carry out operations such as fluorescence dyeing after cultivateing on the device, avoid salvaging the operation of cover glass, avoided making the cover glass turn over, make the fluorochrome add wrong face, cause the dyeing failure simultaneously. If the gel lattice is used, the size of the gel lattice is small, and the dyeing range is reduced, so that the antibody and the fluorescent dye are saved, and the experiment cost is reduced. Use the utility model discloses the device carries out sample fluorescence dyeing, just can direct observation with the buffer solution in addition, has left out the step of adding the mounting plate liquid, scribbling the nail polish, has practiced thrift the time of system appearance. Meanwhile, the nail polish is prevented from permeating into the sample, so that the sample is polluted and observation is influenced.
5. can be used for micromanipulation. The utility model discloses the device has reduced the height that carries the appearance device, and the height of device can be designed into 0.2-0.5cm, is ordinary one fifth to one half of carrying the appearance device height to increase the inclination and the migration range of micro-manipulation needle.
6. The upper plate and the lower plate can be made of high-density stainless steel, aluminum alloy and the like, so that the weight of the sample loading device is increased. The weight of the sample carrying device is increased, and the visual field is not easy to drift. This is particularly advantageous in microscopic observation under a high power objective of an inverted microscope, especially in multi-point repetitive observation.
Drawings
FIG. 1 is a conventional cell culture dish of the prior art;
FIG. 2 is a prior art glass substrate;
Fig. 3 is a schematic view of a sample loading device according to an embodiment of the present invention.
Description of reference numerals:
1-upper plate; 2-a gasket; 3-cover glass; 4-lower plate; 5-buckling; 6-glue grid.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings.
As shown in fig. 1, the sample loading device for microscope of the present invention comprises: an upper plate 1, a gasket 2, a cover glass 3, and a lower plate 4, the upper plate 1 and the lower plate 4 being connected by a hinge. The upper plate and the lower plate are connected into a whole by adopting a hinge structure, and the upper plate and the lower plate can be used for microscope operation after being attached after a sample is loaded, so that the operation is very simple and convenient.
The upper plate 1 may have any shape such as a rectangle, a square, a circle, etc., and is provided with a through hole. The shape of the through hole can be round, square or rectangular. The number of the through holes may be one, two or more. In one embodiment, as shown in fig. 1, the upper plate 1 is provided with a circular through hole. In further embodiments, the number of circular through holes may be two or more.
There may also be graduations or numbers around the through-hole for accurately locating the viewing area to find a previously viewed area. For example, in one embodiment, the upper plate 1 has a square through hole in the center, the size of the through hole is 4 × 4cm, and the periphery of the through hole is marked with numbers.
The gasket 2 is arranged on the lower surface of the upper plate 1 and surrounds the through hole on the upper plate 1, and the gasket 2 is made of flexible materials and plays a role in sealing, so that the sealing performance of the device can be improved. Preferably, the gasket 2 can be fixedly mounted on the upper plate 1 by means of fitting, sticking and the like, so that time can be saved, and the operation is simpler and more convenient. The shape of the gasket 2 is the same as that of the through hole of the upper plate 1, and the size is slightly larger than that of the through hole of the upper plate 1. As shown in fig. 1, in one embodiment, the washer 2 has a circular ring shape with an inner diameter slightly larger than that of the circular through hole. In another embodiment, the upper plate 1 has a square through hole in the center, and the washer 2 is also square and has a size slightly larger than the size of the square through hole.
The cover glass 3 is placed on the through hole of the lower plate 4 to carry the sample. The cover glass 3 may be circular, rectangular or square in shape. In one embodiment, the cover glass 3 is 4.5X 2.5cm in size.
The shape of the lower plate 4 may be the same as or different from that of the upper plate 1, and the size thereof may be the same as or larger than that of the upper plate 1, and the lower plate 4 is also provided with through holes, the size, position, number and shape of which correspond to those of the upper plate 1.
The material of the upper plate 1 and the lower plate 4 may be selected from plastic, resin, acryl, silicone, rubber, ABS, nylon, stainless steel, aluminum alloy, or the like.
The sizes of the through holes of the upper plate 1 and the lower plate 4 and the gasket 2 can be designed into different specifications according to the size of the cover glass 3 and are slightly smaller than the cover glass 3.
in one embodiment, the edge of the lower plate 4 is provided with a catch 5, and when the upper plate 1 and the lower plate 4 are in a fitting state, the catch 5 can catch the edge of the upper plate 1. The number of the snap 5 may be one or more, preferably two. Of course, the catch 5 can also be provided on the upper plate 1.
In one embodiment, the sample carrier device may further comprise a glue grid 6, the glue grid 6 being made of a flexible material and having one or more through holes, the glue grid 6 being attachable to the coverslip 3 by means of an adhesive, separating the coverslip 3 into separate compartments. Preferably, the gel 6 has a plurality of through holes, and the cover glass 3 can be divided into a plurality of chambers so as to load different samples. The shape of the glue grid 6 can be square, rectangular or circular, etc. In one embodiment, the size of the glue grid 6 is 0.5 x 0.5 cm. The space between the scales or numbers around the through hole of the upper plate 1 is consistent with the size of the glue lattice 6, and the glue lattice position is marked. The material of the gasket 2 and the rubber grid 6 is not limited, and can be flexible material, such as silica gel, rubber and the like.
The upper plate 1, the gasket 2, the lower plate 4 and the rubber lattice 6 can be used repeatedly after being sterilized, and cost is saved.
The utility model discloses a carry appearance device is applicable to the observation of ordinary microscope, laser scanning confocal microscope etc.. The sample can be directly observed by adding the buffer solution through sample preparation steps such as fluorescent dyeing and the like, so that the steps of adding the sealing piece solution and coating nail polish are omitted, and the sample preparation time is saved. And the nail polish is prevented from permeating into the sample, so that the sample is prevented from being polluted and the observation is prevented from being influenced.
The utility model discloses the device also can directly be used for operations such as fluorescence dyeing, has avoided the operation of salvaging the cover glass, has avoided making the cover glass turn over simultaneously, has made the fluorochrome add wrong face, has caused the dyeing failure. If the gel lattice is used, the size of the gel lattice is small, and the dyeing range is reduced, so that the antibody and the fluorescent dye are saved, and the experiment cost is reduced. Use the utility model discloses the device carries out sample fluorescence dyeing, just can direct observation with the buffer solution in addition, has left out the step of adding the mounting plate liquid, scribbling the nail polish, has practiced thrift the time of system appearance. Meanwhile, the nail polish is prevented from permeating into the sample, so that the sample is polluted and observation is influenced.
The utility model discloses a carry appearance device is applicable to the micro-operation, has reduced the thickness that carries appearance device, increases the inclination and the range of movement of micro-operation needle.
The utility model discloses a carry appearance device also is suitable for the observation of suspension cell. The cover slip can be coated with polylysine, and the suspension cells can be added dropwise and observed with a microscope.
The sample carrying device of the utility model can also be used for cell culture. Because the leakproofness is good, can use the utility model discloses cultivate the cell, arrange the incubator in and cultivate the cell. Compared with a common culture dish, the size of the gel is small, so that the cell growth space is reduced, the reagents such as culture medium, growth factors and the like are greatly saved, and the experiment cost is reduced. Meanwhile, different samples are separated by the glue lattice, and a plurality of samples can be processed on one cover glass simultaneously, so that the incubation conditions of different samples are consistent, and the univariate research is ensured.
The utility model discloses a carry appearance device except that the coverslip, all the other parts all can use repeatedly, greatly reduced purchases the cost of culture dish. Meanwhile, the cover glass is used as a part for bearing a sample and is disposable, so that pollution caused by unclean cleaning is avoided. The utility model can avoid pollution and reduce cost.
The above-mentioned embodiments, further detailed description of the objects, technical solutions and advantages of the present invention, it should be understood that the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the present invention, and any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. A sample loading device for a microscope, comprising: the glass cover comprises an upper plate, a gasket, a cover glass and a lower plate, wherein one or more through holes are formed in the upper plate; the gasket is fixed on the lower surface of the upper plate and surrounds the through hole on the upper plate; the lower plate is connected with the upper plate through a hinge and is provided with one or more through holes, and the shape, size and position of the through hole on the lower plate are consistent with those of the through hole on the upper plate; the cover glass is placed on the through hole of the lower plate and used for bearing a sample.
2. The sample carrier of claim 1, wherein the through-holes in the upper plate and the through-holes in the lower plate are circular, rectangular, or square in shape.
3. The sample carrier of claim 1, wherein the upper and lower plates are made of a material selected from the group consisting of plastic, resin, acrylic, silicone, rubber, ABS, nylon, stainless steel, and aluminum alloy.
4. The sample carrier of claim 1, wherein the through-hole of the upper plate is surrounded by a scale or a number.
5. The loading device of claim 1, wherein the gasket is made of a flexible material selected from silicone or rubber.
6. The sample carrier of claim 1, further comprising a gel grid having one or more through-holes, the gel grid being secured to the cover slip for forming one or more chambers on the cover slip.
7. The loading device of claim 6, wherein the gel grid is square, rectangular, or circular in shape.
8. The sample carrier according to claim 6, wherein the gel lattice is made of a flexible material selected from silicone or rubber.
9. The sample loading device according to claim 1, wherein the lower plate is provided with a buckle, and when the upper plate and the lower plate are in a fit state, the buckle can clamp the upper plate.
10. The sample loading device according to claim 1, wherein a buckle is provided on the upper plate, and the buckle can clamp the lower plate when the upper plate and the lower plate are in a fit state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920744720.7U CN209784649U (en) | 2019-05-22 | 2019-05-22 | Sample carrying device for microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920744720.7U CN209784649U (en) | 2019-05-22 | 2019-05-22 | Sample carrying device for microscope |
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| Publication Number | Publication Date |
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| CN209784649U true CN209784649U (en) | 2019-12-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201920744720.7U Active CN209784649U (en) | 2019-05-22 | 2019-05-22 | Sample carrying device for microscope |
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| CN (1) | CN209784649U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110894466A (en) * | 2019-12-20 | 2020-03-20 | 广州海润康华生物科技有限公司 | Culture medium for stem cell culture |
-
2019
- 2019-05-22 CN CN201920744720.7U patent/CN209784649U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110894466A (en) * | 2019-12-20 | 2020-03-20 | 广州海润康华生物科技有限公司 | Culture medium for stem cell culture |
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