CN203602629U - Spring presser movable-bottom cell culture dish - Google Patents
Spring presser movable-bottom cell culture dish Download PDFInfo
- Publication number
- CN203602629U CN203602629U CN201320737775.8U CN201320737775U CN203602629U CN 203602629 U CN203602629 U CN 203602629U CN 201320737775 U CN201320737775 U CN 201320737775U CN 203602629 U CN203602629 U CN 203602629U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- compressing tablet
- dish body
- chamber seat
- processed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a spring presser movable-bottom cell culture dish which is characterized in that two bottom cavity bases inclining towards the center of the lower bottom surface of the culture dish body are symmetrically processed both sides of the bottom surface; a presser is inserted into the two bottom cavity bases respectively; moreover, a through hole and a groove surface are formed in the center of the bottom surface of the culture dish body. The spring presser movable-bottom cell culture dish is technically characterized in that cover glass for culturing cells can be arranged on the cell culture dish conveniently through the pressers, and circular and square cover glass of different diameters or side lengths can be clamped.
Description
Technical field
The utility model relates to a kind of Novel cell culture dish, specifically, is a kind of spring pressuring plate dump bottom Tissue Culture Dish for experimental study and medical context of detection.
Background technology
Cell cultures refers to from in-vivo tissue takes out cell in vitro simulated in vivo environment, makes its growth and breeding, and maintains a kind of culture technique of its structure and function.
At present, doing special detection at viable cell need to be when the microscope lens,oil immersion, because biomicroscope is inverted microscope mostly, camera lens will remove to see clearly the viable cell on culture dish through culture dish bottom from lower.General Tissue Culture Dish bottom is blocked up, can not meet the requirement that lens,oil immersion focal length is little, therefore common Tissue Culture Dish can not be used for the microscopical observation of this viable cell.
Therefore, need a kind of Tissue Culture Dish of using for the viable cell microscopic of experimental study and medical context of detection.
Summary of the invention
The purpose of this utility model is to provide a kind of spring pressuring plate dump bottom Tissue Culture Dish simple in structure.
In order to address the above problem, the technical solution of the utility model is: a kind of spring pressuring plate dump bottom Tissue Culture Dish, is characterized in that: be processed with two in opposite directions towards bottom center, inclination 5 ~ 10 in the bottom surface zygomorphy of culture dish body
obottom chamber seat; Two bottom chamber seats are towards a side of bottom center, have the notch that is parallel to bottom surface, respectively be inserted with a compressing tablet, the length of the half of compressing tablet is in bottom chamber seat the inside, the compressing tablet of second half length stretches out bottom chamber seat, and two compressing tablets that stretch out under the effect of tilting at bottom chamber seat are pressed on the bottom surface of culture dish body in opposite directions; Centre in bottom chamber seat is processed with compressing tablet seesaw support, and compressing tablet is through on the downward one end of compressing tablet seesaw support, and compressing tablet can be lifted up up and down left and right in compressing tablet seesaw support; One end at compressing tablet seesaw support towards culture dish body side surface, has installed a main spring downwards by the bottom surface of culture dish body, by this end of compressing tablet to pressing down; At the other end of compressing tablet seesaw support, by the bottom surface of bottom chamber seat, a holddown spring is upwards installed, compressing tablet is upwards pressed on to the bottom surface of culture dish body.The design of compressing tablet seesaw support and main spring, holddown spring, can make compressing tablet one end lift up from the bottom surface of culture dish body under external force, after external force is withdrawn, compressing tablet again can automatic impaction on the bottom surface of culture dish body.
In technique scheme, the center of the bottom surface of described culture dish body, is processed with a through hole, is processed with a groove surface square, the degree of depth >=0.3mm around bottom surface through hole.This is designed with to be beneficial to lays cover glass on groove surface, meanwhile, lowers the bottom thickness of culture dish body.
In technique scheme, the part that described compressing tablet stretches out is to be upwards pressed on the groove surface of bottom surface of culture dish body; Described groove surface can be also circular.Square and circular two kinds of designs, are conducive to the use of the cover glass of different size and present situation.
The utility model technical characterstic is that the cover glass of culturing cell can be arranged on Tissue Culture Dish by compressing tablet easily, and can clamp circle or the square cover glass of different diameter specification or length of side specification.The highly versatile of Tissue Culture Dish.
Compared with prior art, the utlity model has following beneficial effect:
Available common culture dish on cover glass, in microscopic examination process, is clamped to cell cultures on the utility model by cover glass by compressing tablet, realize and detect viable cell sample, greatly reduce testing cost, improved detection efficiency, facilitated the observation of the inverted microscope of viable cell.
Accompanying drawing explanation
Fig. 1 is that master of the present utility model looks cross-sectional view;
Fig. 2 looks schematic perspective view under bottom of the present utility model;
Fig. 3 looks schematic diagram on bottom of the present utility model;
In figure: 1. bottom chamber seat; 2. culture dish body; 3. through hole; 4. groove surface; 5. main spring; 6. compressing tablet seesaw support; 7. holddown spring; 8. compressing tablet; 9. cover glass.
Embodiment
Further the utility model is illustrated below in conjunction with drawings and Examples.
Specific embodiment one
Referring to Fig. 1 to Fig. 3: spring pressuring plate dump bottom Tissue Culture Dish, the bottom surface zygomorphy of culture dish body 2 be processed with two in opposite directions towards bottom center, tilt 5 ~ 10
obottom chamber seat 1; Two bottom chamber seats 1 are towards a side of bottom center, have the notch that is parallel to bottom surface, respectively be inserted with a compressing tablet 8, the length of the half of compressing tablet 8 is in bottom chamber seat 1 the inside, the compressing tablet 8 of second half length stretches out bottom chamber seat 1, and two compressing tablets 8 that stretch out under the effect of tilting at bottom chamber seat 1 are pressed on the bottom surface of culture dish body 2 in opposite directions; Be processed with compressing tablet seesaw support 6 in the centre of 1 li of bottom chamber seat, compressing tablet 8 is arranged on compressing tablet seesaw support 6, and compressing tablet 8 can be lifted up up and down in 6 li of left and right of compressing tablet seesaw support; In compressing tablet seesaw support 6 one end towards culture dish body 2 sides, by the bottom surface of culture dish body 2, a main spring 5 has been installed downwards, by this end of compressing tablet 8 to pressing down; At the other end of compressing tablet seesaw support 6, by the bottom surface of bottom chamber seat 1, a holddown spring 7 is upwards installed, compressing tablet 8 is upwards pressed on to the bottom surface of culture dish body 2.And, at the center of the bottom surface of culture dish body 2, be processed with a through hole 3 diameter of phi=8mm, around bottom surface through hole 3, be processed with 25 × 25 smooth mm square, the groove surface 4 of the degree of depth=0.3mm.
When use, break respectively two compressing tablets 8 into two with one's hands, now compressing tablet 8 upwarps around compressing tablet seesaw support 6 one end, and compressing tablet 8 leaves the groove surface 4 of the bottom surface of culture dish body 2, and main spring 5 and holddown spring 7 are simultaneously stressed compressed; Then, the cover glass 9 of diameter of phi=18mm circle of cultured cell, cell is placed on the centre of culture dish lower bottom part groove surface 4 upward, unclamps compressing tablet 8, under the reactive force that compressing tablet 8 automatically replies at main spring 5 and holddown spring 7, cover glass 9 is tightly pressed on groove surface 4.Process smooth and cover glass and be affixed closely due to groove surface 4, subsequently, in culture dish body 2, add cell culture fluid, can leakage.Finally build culture dish lid, the culture dish assembling is taken to microscope place, carry out viable cell detection.
After having detected, siphon away nutrient solution, change cover glass 9, be reentered into cover glass to be detected 9, detect the viable cell sample on second cover glass 9 by aforementioned operation method.
Specific embodiment two
Referring to Fig. 1 to Fig. 3: spring pressuring plate dump bottom Tissue Culture Dish, the bottom surface zygomorphy of culture dish body 2 be processed with two in opposite directions towards bottom center, tilt 5 ~ 10
obottom chamber seat 1; Two bottom chamber seats 1 are towards a side of bottom center, have the notch that is parallel to bottom surface, respectively be inserted with a compressing tablet 8, the length of the half of compressing tablet 8 is in bottom chamber seat 1 the inside, the compressing tablet 8 of second half length stretches out bottom chamber seat 1, and two compressing tablets 8 that stretch out under the effect of tilting at bottom chamber seat 1 are pressed on the bottom surface of culture dish body 2 in opposite directions; Be processed with compressing tablet seesaw support 6 in the centre of 1 li of bottom chamber seat, compressing tablet 8 is arranged on compressing tablet seesaw support 6, and compressing tablet 8 can be lifted up up and down in 6 li of left and right of compressing tablet seesaw support; In compressing tablet seesaw support 6 one end towards culture dish body 2 sides, by the bottom surface of culture dish body 2, a main spring 5 has been installed downwards, by this end of compressing tablet 8 to pressing down; At the other end of compressing tablet seesaw support 6, by the bottom surface of bottom chamber seat 1, a holddown spring 7 is upwards installed, compressing tablet 8 is upwards pressed on to the bottom surface of culture dish body 2.And, at the center of the bottom surface of culture dish body 2, be processed with a through hole 3, the diameter of phi=10mm in hole, through hole 3 bottom surface is around processed with the groove surface 4 of smooth Φ=35mm, the degree of depth=0.5mm.
When use, break respectively compressing tablet 8 into two with one's hands, cover glass square 22 × 22 mm of cultured cell 9, cell is placed in the middle of the groove surface 4 of bottom upward, compresses with compressing tablet 8.In culture dish body 2, add cell culture fluid again, build culture dish lid.Finally the culture dish assembling is taken to microscope place, carry out viable cell detection.
After having detected, siphon away nutrient solution, change cover glass 9, be reentered into cover glass to be detected 9, detect the viable cell sample on second cover glass 9 by aforementioned operation method.
Special instruction: the material of utility model is not limited by this specification sheets exemplary plot, and every principle of design and the different Tissue Culture Dish of same or analogous from this patent, the used material of each element, all in the protection domain of this patent.
Claims (3)
1. a spring pressuring plate dump bottom Tissue Culture Dish, is characterized in that: be processed with two in opposite directions towards bottom center, inclination 5 ~ 10 in the bottom surface zygomorphy of culture dish body (2)
obottom chamber seat (1); Two bottom chamber seats (1) are towards a side of bottom center, have the notch that is parallel to bottom surface, respectively be inserted with a compressing tablet (8), the length of the half of compressing tablet (8) is in bottom chamber seat (1) the inside, the compressing tablet (8) of second half length stretches out bottom chamber seat (1), and two compressing tablets (8) that stretch out under the effect of tilting at bottom chamber seat (1) are pressed on the bottom surface of culture dish body (2) in opposite directions; Be processed with compressing tablet seesaw support (6) in the inner centre of bottom chamber seat (1), compressing tablet (8) is through on the downward one end of compressing tablet seesaw support (6), and compressing tablet (8) can be lifted up up and down in the inner left and right of compressing tablet seesaw support (6); One end at compressing tablet seesaw support (6) towards culture dish body (2) side, has installed a main spring (5) downwards by the bottom surface of culture dish body (2), by this end of compressing tablet (8) to pressing down; At the other end of compressing tablet seesaw support (6), by the bottom surface of bottom chamber seat (1), a holddown spring (7) is upwards installed, compressing tablet (8) is upwards pressed on to the bottom surface of culture dish body (2).
2. spring pressuring plate dump bottom Tissue Culture Dish according to claim 1, it is characterized in that: the center of the bottom surface of described culture dish body (2), be processed with a through hole (3), around bottom surface through hole (3), be processed with a groove surface (4) square, the degree of depth >=0.3mm.
3. spring pressuring plate dump bottom Tissue Culture Dish according to claim 1, is characterized in that: its part of stretching out of described compressing tablet (8) is to be upwards pressed on the groove surface (4) of bottom surface of culture dish body (2); Described groove surface (4) can be also circular.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320737775.8U CN203602629U (en) | 2013-11-21 | 2013-11-21 | Spring presser movable-bottom cell culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320737775.8U CN203602629U (en) | 2013-11-21 | 2013-11-21 | Spring presser movable-bottom cell culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203602629U true CN203602629U (en) | 2014-05-21 |
Family
ID=50715316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320737775.8U Withdrawn - After Issue CN203602629U (en) | 2013-11-21 | 2013-11-21 | Spring presser movable-bottom cell culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203602629U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103589640A (en) * | 2013-11-21 | 2014-02-19 | 重庆科技学院 | Cell culture dish with pressing sheets pressed on movable bottom by springs |
| CN113655059A (en) * | 2021-09-02 | 2021-11-16 | 中国科学院长春应用化学研究所 | Sample liquid phase imaging device |
-
2013
- 2013-11-21 CN CN201320737775.8U patent/CN203602629U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103589640A (en) * | 2013-11-21 | 2014-02-19 | 重庆科技学院 | Cell culture dish with pressing sheets pressed on movable bottom by springs |
| CN103589640B (en) * | 2013-11-21 | 2015-03-04 | 重庆科技学院 | Cell culture dish with pressing sheets pressed on movable bottom by springs |
| CN113655059A (en) * | 2021-09-02 | 2021-11-16 | 中国科学院长春应用化学研究所 | Sample liquid phase imaging device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201695031U (en) | Partitioned culture vessel with glass bottom | |
| CN202322860U (en) | High-resolution imaged cell culture dish with glass bottom | |
| CN101992136B (en) | Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells | |
| CN203602629U (en) | Spring presser movable-bottom cell culture dish | |
| CN202177536U (en) | Sheet production auxiliary equipment provided with natural settling device and used in liquid-based cytology sheet production system | |
| CN103589640B (en) | Cell culture dish with pressing sheets pressed on movable bottom by springs | |
| CN203530338U (en) | Culture dish for observing living cells through microscope | |
| CN102443540A (en) | Detachable cell culture chamber | |
| CN107828640B (en) | Microorganism collector in water | |
| CN203546037U (en) | Movable glass bottom cell culture dish | |
| CN211453597U (en) | Quick water quality testing device | |
| CN203429184U (en) | Multi-adherent-cell coculture device | |
| CN204417516U (en) | The special Micro-CPE neutralization test ware of laser scanning co-focusing microscope | |
| CN104630062A (en) | Cover glass fixing card for cell culture | |
| CN203546036U (en) | Assembled culture dish for living cell microscopic observation | |
| CN203569115U (en) | Slide structure of slide culture dish | |
| CN204779610U (en) | Cover glass | |
| CN203668416U (en) | Cover glass fixing clamp for cell culture | |
| CN213364568U (en) | Living cell confocal imaging chamber | |
| CN107723238B (en) | Cell culture device and application thereof | |
| CN111748454A (en) | Substance separating device with high extraction rate and high transfer rate | |
| CN103620018B (en) | Cell cultivator | |
| CN103589639A (en) | Mobile type cell culture dish with glass bottom | |
| CN215828808U (en) | Cell culture vessel convenient for taking and placing glass slides | |
| CN205856468U (en) | Tissue Culture Dish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140521 Effective date of abandoning: 20150304 |
|
| RGAV | Abandon patent right to avoid regrant |