CN101992136B - Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells - Google Patents
Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells Download PDFInfo
- Publication number
- CN101992136B CN101992136B CN201010500097A CN201010500097A CN101992136B CN 101992136 B CN101992136 B CN 101992136B CN 201010500097 A CN201010500097 A CN 201010500097A CN 201010500097 A CN201010500097 A CN 201010500097A CN 101992136 B CN101992136 B CN 101992136B
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- Prior art keywords
- cell lump
- collecting ring
- filtration membrane
- tube
- push pipe
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 239000012189 cellwax Substances 0.000 title abstract 2
- 239000012528 membrane Substances 0.000 claims abstract description 50
- 238000001914 filtration Methods 0.000 claims abstract description 48
- 239000004575 stone Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 abstract description 6
- 238000000926 separation method Methods 0.000 abstract description 6
- 239000007790 solid phase Substances 0.000 abstract description 2
- 239000013049 sediment Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 description 11
- 239000000463 material Substances 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000004568 cement Substances 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000741 silica gel Substances 0.000 description 3
- 229910002027 silica gel Inorganic materials 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
- 150000003738 xylenes Chemical class 0.000 description 1
Images
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- Centrifugal Separators (AREA)
Abstract
The invention discloses a combined centrifuge tube for preparing cell wax blocks by using exfoliated cells. The combined centrifuge tube comprises an ejection tube and is characterized in that: the upper and lower ends of the ejection tube are open, and the lower part of the ejection tube is provided with a screw thread or a clamping groove; the lower part of the ejection tube is connected with a cell block collecting ring, the upper and lower ends of the cell block collecting ring are open, and the lower part of the cell block collecting ring is connected with a filtration membrane; the ejection tube is connected with a bottom tube through the screw thread or the clamping groove, the upper end of the bottom tube is open, and the lower end of the bottom tube is closed; and the cell block collecting ring and the filtration membrane are positioned in the ejection tube and the bottom tube. The combined centrifuge tube is simple in operation, can be directly used for solid-liquid separation and endows the solid-phase sediment with a shape of certain specification at the same time, can be used for conveniently taking the complete cell blocks out, and is convenient for next operation.
Description
Technical field
The present invention relates to a kind ofly be used to make the combined centrifugal pipe that the cell lump wax stone uses for medical speciality is used to separate the preparation cast-off cells.
Background technology
The exfoliative cytology inspection is the important component part of medical pathologies and medical test, generally adopts the main means of the smear of variety of way as inspection, in real work; Many positives, false positive and boundary property smear need the patient further to draw materials or check, and the previous exfoliative cytology sample of getting is except that repeating to make the smear; Owing to lack the means of ripe making cytology wax stone; Generally all discarded, the present invention provides a kind of combined centrifugal pipe that the preparation cast-off cells are used to make the cell lump wax stone that separates, can be with the above-mentioned smear that need further draw materials or check; Utilize original exfoliative cytology sample; Be made as cytology wax stone, further verify and/or clarify a diagnosis, improve correct diagnosis with certain canonical schema.
At present; The used centrifuge tube of medical institutions is generally a monoblock type centrifuge tube; Utilize centrifuge to make that solid matter is deposited into the purpose that the pipe end reaches Separation of Solid and Liquid in the suspension; But still there is directly contact in the solid matter that is deposited on the pipe end with liquid phase substance, can't it kept the certain volume shape and not have complete taking-up under the situation of unnecessary liquid phase composition as far as possible.
Summary of the invention
The purpose of this invention is to provide a kind of cast-off cells prepares the cell lump wax stone and uses the combined centrifugal pipe; Solve in the actual mechanical process, the solid matter piece that centrifugal back cell deposition thing forms can not accomplish that solid-liquid separates separately, the problem that the cell lump deposit can't take out separately from pipe; Make the solid precipitation thing in pipe, can accomplish Separation of Solid and Liquid; Give the shape that the solid precipitation thing has certain standardization simultaneously, can take out easily, get into next step operation.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is:
A kind of cast-off cells prepare the cell lump wax stone and use the combined centrifugal pipe, comprise push pipe, it is characterized in that: the upper and lower side opening of push pipe is threaded or draw-in groove on the outer wall of push pipe bottom; The bottom of push pipe is connected with the cell lump collecting ring, the upper and lower side opening of cell lump collecting ring, and the bottom of cell lump collecting ring is connected with filtration membrane; Push pipe is connected with bottom tube through screw thread or draw-in groove, the open upper end of bottom tube, lower end closed; The top bulge loop of described cell lump collecting ring partly is positioned at push pipe and bottom tube, and the remainder and the filtration membrane of cell lump collecting ring are positioned at bottom tube.
Prepare the cell lump wax stone according to described cast-off cells and use the combined centrifugal pipe, it is characterized in that: be connected with the filtration membrane supporting ring below the said filtration membrane.
Prepare the cell lump wax stone according to described cast-off cells and use the combined centrifugal pipe; It is characterized in that: correspondence is threaded or draw-in groove on the inwall of said bottom tube; On the tube wall of the screw thread of bottom tube or draw-in groove below annular platform is arranged, place described cell lump collecting ring, filtration membrane, filtration membrane supporting ring on the annular platform.
Prepare the cell lump wax stone according to described cast-off cells and use the combined centrifugal pipe; It is characterized in that: said cell lump collecting ring top is bulge loop; The bottom is a seat, the inwall socket connection of the outer wall of bulge loop and push pipe bottom, and the outer wall of cell lump collecting ring lower seat is provided with anti-skidding line.
The invention has the advantages that: simple to operate, directly accomplish Separation of Solid and Liquid and give the shape that the solid precipitation thing has certain standardization simultaneously that cell lump is easy to take out, and is convenient to next step operation.
Description of drawings
Fig. 1 is a decomposition texture sketch map of the present invention.
Fig. 2 is a decomposition texture cutaway view of the present invention.
Fig. 3 is the structural representation after the present invention assembling.
In the accompanying drawing: 1, push pipe; 2, cell lump collecting ring; 3, filtration membrane; 4, bottom tube; 5, filtration membrane supporting ring.
The specific embodiment
Below in conjunction with accompanying drawing and specific embodiment the present invention is described further:
The present invention, cast-off cells prepare the cell lump wax stone with combined centrifugal pipe such as Fig. 1, Fig. 2, shown in Figure 3, comprise push pipe 1, and the upper and lower side opening of push pipe 1 is threaded or draw-in groove on the outer wall of push pipe 1 bottom, and correspondence is threaded or draw-in groove on the inwall of bottom tube 4; Push pipe 1 is connected the open upper end of bottom tube 4, lower end closed through screw thread or draw-in groove with bottom tube 4.
The bottom of push pipe 1 is connected with cell lump collecting ring 2; The upper and lower side opening of cell lump collecting ring 2, cell lump collecting ring 2 tops are bulge loop, the bottom is a seat; The inwall socket connection of the outer wall of bulge loop and push pipe 1 bottom, the outer wall of cell lump collecting ring 2 lower seat is provided with anti-skidding line.
The bottom of cell lump collecting ring 2 is connected with filtration membrane 3, is connected with filtration membrane supporting ring 5 below the filtration membrane 3, and filtration membrane supporting ring 5 can be made by plastic cement or silica gel.
On the tube wall of the screw thread of bottom tube 4 or draw-in groove below annular platform is arranged, place described cell lump collecting ring 2, filtration membrane 3, filtration membrane supporting ring 5 on the annular platform.
The top bulge loop of cell lump collecting ring 2 partly is positioned at push pipe 1 and bottom tube 4, and the remainder of cell lump collecting ring 2 and filtration membrane 3 are positioned at bottom tube 4.Annular platform supports the filtration membrane supporting ring in order to connect the back with push pipe.
Cast-off cells of the present invention prepare the cytology wax stone with the combined centrifugal pipe by push pipe 1, cell lump collecting ring 2, filtration membrane 3, bottom tube 4, filtration membrane supporting ring 5 totally five the part form.As shown in Figure 3, after spinning, push pipe and bottom tube make push pipe, cell lump collecting ring, filtration membrane, filtration membrane supporting ring, bottom tube form a unified integral body.
Cell lump collecting ring 2 tops are open, and the corresponding push pipe bottom of inserting with the push pipe bottom forms plug-in type with push pipe and is connected; The bottom outer wall is provided with the anti-skidding line of vertical arrangement; Be convenient to separate with push pipe in the subsequent operation, the non-fastness of bottom and filtration membrane is bonding, as adopting adhesive sticker bonding.Filtration membrane can separate under certain external force effect with the cell lump collecting ring in the subsequent operation process, is convenient to take out cell lump.
Annotate: cell lump collecting ring and filtration membrane, filtration membrane supporting ring material need organic solvents such as anti-absolute ethyl alcohol, xylenes to soak.
In the operation, cell suspension injects push pipe, after centrifugal; Separation of Solid and Liquid is taken off cell lump collecting ring and filtration membrane, and cell lump promptly is positioned at it; Can get into the wax stone production process jointly and handle, when embedding, take off filtration membrane, can take out complete cell lump easily.
This centrifuge tube is that medical speciality is used for separating the preparation cast-off cells and is used to make the cell lump wax stone and uses; According to what of cell quantity in the cell suspension; Select to use the centrifuge tube of different bores, make the cell lump of making have suitable thickness, guarantee slice-making quality.Cell suspension (being generally the cell suspension that for the first time centrifugal back concentrates) with suitable dosage during use injects push pipe; The centrifuge that the level of putting into is centrifugal, centrifugal back liquid phase composition gets into bottom tube through filtration membrane, and the solid phase composition is the solid matter piece of the cell lump collecting ring internal diameter cross section columnar shape of certain rule; Retain on the filtration membrane; Take out centrifuge tube, behind separation push pipe and the bottom tube, the above composition of filtration membrane takes out with push pipe; Separate with bottom tube; Hold anti-skidding line place, tight cell lump collecting ring bottom again, push pipe is separated with cell lump collecting ring, filtration membrane, cell lump collecting ring, the filtration membrane that will contain cell lump are subsequently put into the dehydration of dewatering box entering dewaterer, waxdip jointly; When embedding, behind isolated cell piece collecting ring and the filtration membrane, cell lump dehydration back volume-diminished can be separated with the cell lump collecting ring easily, can embedding production standard cytology wax stone.
Embodiment recited above describes preferred implementation of the present invention; Be not that design of the present invention and protection domain are limited; Under the prerequisite that does not break away from design concept of the present invention; Common engineers and technicians make technical scheme of the present invention in this area various modification and improvement all should fall into protection scope of the present invention.
Claims (4)
1. cast-off cells prepare the cell lump wax stone and use the combined centrifugal pipe, comprise push pipe (1), and it is characterized in that: the upper and lower side opening of push pipe (1) is threaded or draw-in groove on the outer wall of push pipe (1) bottom; The bottom of push pipe (1) is connected with cell lump collecting ring (2), the upper and lower side opening of cell lump collecting ring (2), and the bottom of cell lump collecting ring (2) is connected with filtration membrane (3); Push pipe (1) is connected with bottom tube (4) through screw thread or draw-in groove, the open upper end of bottom tube (4), lower end closed; The top bulge loop of described cell lump collecting ring (2) partly is positioned at push pipe (1) and bottom tube (4), and the remainder of cell lump collecting ring (2) and filtration membrane (3) are positioned at bottom tube (4).
2. cast-off cells according to claim 1 prepare the cell lump wax stone and use the combined centrifugal pipe, it is characterized in that: be connected with filtration membrane supporting ring (5) below the said filtration membrane (3).
3. cast-off cells according to claim 2 prepare the cell lump wax stone and use the combined centrifugal pipe; It is characterized in that: correspondence is threaded or draw-in groove on the inwall of said bottom tube (4); On the tube wall of the screw thread of bottom tube (4) or draw-in groove below annular platform is arranged, place described cell lump collecting ring (2), filtration membrane (3), filtration membrane supporting ring (5) on the annular platform.
4. cast-off cells according to claim 3 prepare the cell lump wax stone and use the combined centrifugal pipe; It is characterized in that: said cell lump collecting ring (2) top is bulge loop; The bottom is a seat; The inwall socket connection of the outer wall of bulge loop and push pipe (1) bottom, the outer wall of cell lump collecting ring (2) lower seat is provided with anti-skidding line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010500097A CN101992136B (en) | 2010-09-30 | 2010-09-30 | Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010500097A CN101992136B (en) | 2010-09-30 | 2010-09-30 | Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101992136A CN101992136A (en) | 2011-03-30 |
| CN101992136B true CN101992136B (en) | 2012-09-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010500097A Expired - Fee Related CN101992136B (en) | 2010-09-30 | 2010-09-30 | Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101992136B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11833279B2 (en) | 2021-06-11 | 2023-12-05 | Astaria Global, LLC | System and method for isolating alpha 2M molecules |
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| CN105699153B (en) * | 2014-04-23 | 2018-04-17 | 杭州电子科技大学 | Self-action cell block making apparatus |
| CN105890943B (en) * | 2014-04-23 | 2019-01-25 | 杭州电子科技大学 | Cell block preparation facilities with automatic detection function |
| CN105675373B (en) * | 2014-04-23 | 2018-11-09 | 杭州电子科技大学 | Cell block preparation facilities with fast positioning function |
| CN103954486B (en) * | 2014-04-23 | 2016-06-15 | 杭州电子科技大学 | Prepare the adding pressure type reaction unit of cell block |
| CN104359746B (en) * | 2014-11-19 | 2018-03-16 | 白银市第一人民医院 | Cell mass collector and the method for collecting cell in humoral specimen |
| AT517083B1 (en) | 2015-05-04 | 2016-11-15 | Greiner Bio-One Gmbh | Pick-up assembly, especially for a lower intake |
| CN106179552B (en) * | 2016-07-06 | 2018-09-11 | 江苏科华医疗器械科技有限公司 | A kind of integral type ultra-filtration centrifuge tube |
| CN106979881A (en) * | 2017-05-18 | 2017-07-25 | 桂林医学院 | A kind of centrifuge tube prepared for cell block |
| CN110038434A (en) * | 2019-04-23 | 2019-07-23 | 卢彩婷 | A kind of assemble method of dialysis apparatus and the dialysis apparatus |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4534863A (en) * | 1984-05-22 | 1985-08-13 | Schleicher & Schuell, Inc. | Centrifugal filtering device and filter unit therefor |
| DE3722562A1 (en) * | 1987-07-08 | 1989-01-19 | Andreas Szabados | Annular filter body |
| GB2332158A (en) * | 1995-08-28 | 1999-06-16 | Millipore Investment Holdings Limited | Centrifuge sample tube with adsorptive membrane |
| CN201163254Y (en) * | 2008-02-04 | 2008-12-10 | 张震 | Liquid based thin layer cytology inspection apparatus |
| CN201200932Y (en) * | 2008-06-02 | 2009-03-04 | 石国清 | Improved centrifuge tube |
| CN201413266Y (en) * | 2009-06-29 | 2010-02-24 | 石国清 | Cell smearing device |
| CN201825948U (en) * | 2010-09-30 | 2011-05-11 | 何洪敏 | Combined centrifuge tube for preparing cell blocks by using cast-off cells |
-
2010
- 2010-09-30 CN CN201010500097A patent/CN101992136B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4534863A (en) * | 1984-05-22 | 1985-08-13 | Schleicher & Schuell, Inc. | Centrifugal filtering device and filter unit therefor |
| DE3722562A1 (en) * | 1987-07-08 | 1989-01-19 | Andreas Szabados | Annular filter body |
| GB2332158A (en) * | 1995-08-28 | 1999-06-16 | Millipore Investment Holdings Limited | Centrifuge sample tube with adsorptive membrane |
| CN201163254Y (en) * | 2008-02-04 | 2008-12-10 | 张震 | Liquid based thin layer cytology inspection apparatus |
| CN201200932Y (en) * | 2008-06-02 | 2009-03-04 | 石国清 | Improved centrifuge tube |
| CN201413266Y (en) * | 2009-06-29 | 2010-02-24 | 石国清 | Cell smearing device |
| CN201825948U (en) * | 2010-09-30 | 2011-05-11 | 何洪敏 | Combined centrifuge tube for preparing cell blocks by using cast-off cells |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11833279B2 (en) | 2021-06-11 | 2023-12-05 | Astaria Global, LLC | System and method for isolating alpha 2M molecules |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101992136A (en) | 2011-03-30 |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: He Hongmin Inventor after: Song Jilan Inventor after: He Xin Inventor before: Song Jilan Inventor before: He Hongmin |
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| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: SONG JILAN HE HONGMIN TO: HE HONGMIN SONG JILAN HE XIN |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180614 Address after: 251100, two floor, B block, Qilu science and technology incubator, Qilu hi tech Development Zone, Qihe, Dezhou, Shandong Patentee after: Shandong boson Medical Engineering Technology Co., Ltd. Address before: 277100 Department of Pathology, Zaozhuang municipal hospital, No. 47 Middle Road, Shizhong Road, Shizhong District, Zaozhuang, Shandong Patentee before: He Hongmin |
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| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120905 Termination date: 20190930 |