CN217398869U

CN217398869U - Compatible environmental DNA and microorganism acquisition device

Info

Publication number: CN217398869U
Application number: CN202220543153.0U
Authority: CN
Inventors: 冯杰; 吴申浩; 刘瑛; 谢林伸; 梁绮婷; 陈建义; 李智; 陈晓丹
Original assignee: SHENZHEN ACADEMY OF ENVIRONMENTAL SCIENCES
Current assignee: SHENZHEN ACADEMY OF ENVIRONMENTAL SCIENCES
Priority date: 2022-03-14
Filing date: 2022-03-14
Publication date: 2022-09-09
Anticipated expiration: 2032-03-14

Abstract

Compatible formula environment DNA and microorganism acquisition device solves sampling enrichment efficiency, reduces intensity of labour and accommodation problem, and the scheme is: the method comprises the following steps: accomodate water tank, negative pressure pump and filter and acquire the ware. Accomodate the water tank and include: the negative pressure cavity is communicated with the water containing cavity to form an integral structure of the water containing tank. The negative pressure cavity of the negative pressure pump is communicated. The filter acquirer is connected with the negative pressure cavity at the upper end of the storage water tank in a sealing mode, the water inlet of the filter acquirer is communicated with a water source, and the water outlet of the filter acquirer is communicated with the water containing cavity at the lower end of the storage water tank. The beneficial effects are that: but different specifications filter membranes of adaptation, the filtration that only needs to change the adaptation acquires the ware filter head and can realize different filter membrane switches to different adoption needs such as adaptation environment DNA, macromolecular substance, cell collection and water microorganism select the filter membrane in a flexible way, enrich the experimental result. Greatly increased experiment convenience, improved experiment efficiency and reduced burden of field researchers.

Description

Compatible environmental DNA and microorganism acquisition device

Technical Field

The utility model belongs to the water microorganism enrichment of ecological environment research field, DNA and the microorganism acquisition device that uses such as environment DNA enrichment, especially adopt and filter the compatible formula environment DNA and the microorganism acquisition device that the acquirer acquireed environment DNA and microorganism.

Background

With the improvement of the social requirement on environmental protection, the requirement on water environment quality is changed from reaching the water quality standard to the development of water ecological health, the water ecological investigation and research work in the national range is being spread comprehensively, and the metagenomics and environmental DNA technology is used as a new means for analyzing the biodiversity structure and the biological community composition, has the advantages of low consumption, high efficiency, high sensitivity, no damage to organisms and the like, and has a very wide application prospect in the aspect of water ecological investigation and research.

The two technical means of the metagenomics and the environmental DNA technology are required to enrich the microbial community and the environmental DNA in the sample pretreatment process, the current common enrichment method is vacuum filtration, the filtration membrane is different in general specification according to the demand and the selected subsequent extraction mode, and the replacement of the filtration membrane requires the replacement of the whole set of filtration device, which is time-consuming and labor-consuming. Especially for water ecology field investigation workers, a plurality of sets of filtering devices need to be carried for outdoor sampling on the spot, the carrying weight is increased, and the difficulty of field sampling and enrichment is increased. Therefore, the development and design of a compatible device for acquiring environmental DNA, macromolecular substances, cell collection and water body microorganism, which is used for acquiring the filter acquirer according to different requirements of enrichment and is convenient to replace, is an urgent technical problem in the industry for improving the sampling and enrichment efficiency and reducing the labor intensity and the cost.

SUMMERY OF THE UTILITY MODEL

The utility model discloses an improve environment DNA, macromolecular material, cell collection and water microorganism sampling enrichment efficiency, reduce intensity of labour and accommodation, disclose a compatible formula environment DNA and microorganism acquisition device.

The utility model discloses the technical scheme who realizes that the purpose adopts is:

compatible environmental DNA and microorganism acquisition device, the device includes: accomodate water tank, negative pressure pump and filter and acquire the ware.

The storage water tank comprises: the water storage tank comprises a negative pressure cavity, a water containing cavity, a water outlet with a control switch and a negative pressure exhaust port, wherein the negative pressure cavity is arranged at the upper end of the water storage tank, the water containing cavity is arranged at the lower end of the water storage tank, and the negative pressure cavity and the water containing cavity are communicated to form an integral structure of the water storage tank.

The negative pressure pump is communicated with the negative pressure cavity of the containing water tank through the negative pressure pipe to form a connecting structure for realizing a negative pressure state of the negative pressure cavity through the negative pressure pipe.

The filtration acquirer is in sealing connection with a negative pressure cavity at the upper end of the storage water tank, a water inlet of the filtration acquirer is communicated with a water source, and a water outlet of the filtration acquirer is communicated with a water containing cavity at the lower end of the storage water tank.

The utility model has the advantages that: but different specifications filter membranes among the prior art of adaptation, the filtration that only needs to change the adaptation acquires the ware filter head and can realize different filter membrane switches to different adoption needs such as adaptation environment DNA, macromolecular material, cell collection and water microorganism select the filter membrane in a flexible way, richen experimental result. Greatly increased experiment convenience, improved experiment efficiency and reduced burden of field researchers.

The present invention will be described in detail with reference to the accompanying drawings.

Drawings

Fig. 1 is a schematic structural section view of embodiment 1 of the present invention.

Fig. 2 is a schematic structural section view of embodiment 2 of the present invention.

In the attached drawing, 1, a storage water tank, 1-1 negative pressure cavity, 1-2 water containing cavity, 1-3 water outlet with control switch, 1-4 negative pressure exhaust port, 2 negative pressure pump, 3 bayonet type filter membrane, 3-1 bayonet type filter membrane water inlet, 3-2 post-filter conduit, 4-1 filter paper, 4-2 mesh plate, 4-3 hollow filter membrane shell, 4-4 connecting clamping sleeve, 5 water containing filter cup and 5-1 volume scale.

Detailed Description

A compatible environmental DNA and microorganism acquisition device.

The device includes: accomodate water tank 1, negative pressure pump 2 and filter the acquirer.

Accomodate water tank 1 and be used for the enrichment after, filter out the collection of environment DNA, macromolecular substance, cell collection and water sample of water microorganism.

The negative pressure pump 2 is used for forming negative pressure, so that the flow rate of the water sample flowing into the storage water tank 1 is improved, and the enrichment efficiency is improved.

The filter acquirer is used for water sampling, and environmental DNA, macromolecular substances, cells and water microorganisms are separated during water sample filtering to obtain the environmental DNA, the macromolecular substances, the cells, the water microorganisms and the like.

The storage water tank 1 includes: the water storage tank comprises a negative pressure cavity 1-1, a water containing cavity 1-2, a water outlet with a control switch 1-3 and a negative pressure exhaust port 1-4, wherein the negative pressure cavity 1-1 is arranged at the upper end of the water storage tank 1, the water containing cavity 1-2 is arranged at the lower end of the water storage tank 1, and the negative pressure cavity 1-1 and the water containing cavity 1-2 are communicated to form an integral structure of the water storage tank 1.

The negative pressure pump 2 is communicated with the negative pressure cavity 1-1 of the containing water tank 1 through the negative pressure pipe 2-1 to form a connecting structure for realizing a negative pressure state of the negative pressure pump 2 to the negative pressure cavity 1-1 through the negative pressure pipe 2-1.

The filter acquirer is hermetically connected with a negative pressure cavity 1-1 at the upper end of the storage water tank 1, a water inlet of the filter acquirer is communicated with a water source, and a water outlet of the filter acquirer is communicated with a water containing cavity 1-2 at the lower end of the storage water tank 1.

Contain the water sample of environment DNA or macromolecular substance or the cell that needs to collect or the water microorganism, the water inlet of filtration acquirer gets into the filtration acquirer, under the effect of negative pressure pump 2, the water sample carries out the cell or the water microorganism filtration separation back that environment DNA or macromolecular substance or need collect through the filtration acquirer, be detained in the filtration acquirer, get into in the appearance water cavity 1-2 of accomodating water tank 1 lower extreme with the water sample after the cell or the water microorganism separation of environment DNA or macromolecular substance or need collect.

And (4) disassembling the filter acquirer and extracting environmental DNA or macromolecular substances required to be retained in the filter acquirer or cells or water microorganisms required to be collected. And discharging the wastewater in the storage water tank 1 in the filtering process or after the enrichment is completed.

In the embodiment of the utility model provides an in, filtration acquire ware be standard bayonet type filter membrane 3, bayonet type filter membrane 3 through screw thread and the negative pressure chamber 1-1 sealing connection of accomodating water tank 1 upper end, bayonet type filter membrane water inlet 3-1 and water source intercommunication, bayonet type filter membrane delivery port through the back pipe 3-2 of straining and the appearance water cavity 1-2 intercommunication of accomodating water tank 1 lower extreme.

In example 1, the bayonet filter 3 was an integrated filter manufactured by yikeno corporation of Nanjing.

A water sample enters a filtering inner cavity of the bayonet type filter membrane 3 through a bayonet type filter membrane water inlet 3-1, and after filtering, the water sample rapidly enters a water containing cavity 1-2 at the lower end of the containing water tank 1 under the action of negative pressure in a negative pressure cavity 1-1 at the upper end of the containing water tank 1 through a bayonet type filter membrane water outlet and a filtered conduit 3-2.

In this embodiment 2, the filtration obtaining device is a filter paper type filter membrane, and the filter paper type filter membrane includes: the filter paper cover comprises a filter paper 4-1, a mesh plate 4-2, a hollow filter membrane shell 4-3 and a connecting clamping sleeve 4-4, wherein the mesh plate 4-2 is arranged at the upper end of the hollow filter membrane shell 4-3, the filter paper 4-1 covers the mesh plate 4-2, the connecting clamping sleeve 4-4 is arranged at the upper end of the hollow filter membrane shell 4-3 in a threaded manner, a limiting structure that the filter paper 4-1 covers the mesh plate 4-2 is formed between the connecting clamping sleeve 4-4 and the hollow filter membrane shell 4-3, the connecting clamping sleeve 4-4 enters a water inlet structure of a filtration acquirer in the hollow filter membrane shell 4-3 through the filter paper 4-1 and the mesh plate 4-2 after water filtration, the lower end opening of the hollow filter membrane shell 4-3 is a water outlet of the filtration acquirer, the other end of the connecting clamping sleeve 4-4 is connected with a water containing filter cup 5 through threads, the connecting cutting sleeve 4-4 is communicated with the water containing filter bowl 5 to form a water inlet and water source communicating structure of the filter obtaining device.

After the sampled water in the water containing filter cup 5 is filtered by the filter paper 4-1, environmental DNA or macromolecular substances or cells or water body microorganisms needing to be collected are retained in the filter paper 4-1, and the separated water sample rapidly enters the water containing cavity 1-2 at the lower end of the containing water tank 1 under the negative pressure action in the negative pressure cavity 1-1 of the containing water tank 1 through the mesh plate 4-2. Extracting environmental DNA or macromolecular substances required to be retained on the filter paper 4-1 or cells or water body microorganisms required to be collected. And discharging the wastewater in the storage water tank 1 in the filtering process or after the enrichment is finished.

In the embodiment, the water containing filter bowl 5 is provided with a volume scale 5-1. The method is convenient for counting and calculating the content of the environmental DNA or macromolecular substances or cells or water body microorganisms needing to be collected in water.

Claims

1. Compatible formula environment DNA and microorganism obtain device, its characterized in that:

the device comprises: the device comprises a containing water tank (1), a negative pressure pump (2) and a filtering acquisition device;

the storage water tank (1) comprises: the water storage tank comprises a negative pressure cavity (1-1), a water containing cavity (1-2), a water outlet (1-3) with a control switch and a negative pressure exhaust port (1-4), wherein the negative pressure cavity (1-1) is arranged at the upper end of the water storage tank (1), the water containing cavity (1-2) is arranged at the lower end of the water storage tank (1), and the negative pressure cavity (1-1) and the water containing cavity (1-2) are communicated to form an integral structure of the water storage tank (1);

the negative pressure pump (2) is communicated with a negative pressure cavity (1-1) of the containing water tank (1) through a negative pressure exhaust port (1-4) to form a connecting structure for realizing a negative pressure state of the negative pressure pump (2) on the negative pressure cavity (1-1) through the negative pressure exhaust port (1-4);

the filter acquirer is hermetically connected with a negative pressure cavity (1-1) at the upper end of the storage water tank (1), a water inlet of the filter acquirer is communicated with a water source, and a water outlet of the filter acquirer is communicated with a water containing cavity (1-2) at the lower end of the storage water tank (1).

2. The apparatus for obtaining compatible environmental DNA and microorganisms according to claim 1, wherein: the filtration acquire the ware for bayonet type filter membrane (3), bayonet type filter membrane (3) through screw thread and negative pressure chamber (1-1) sealing connection of accomodating water tank (1) upper end, bayonet type filter membrane water inlet (3-1) and water source intercommunication, bayonet type filter membrane delivery port is through pipe (3-2) and the appearance water cavity (1-2) intercommunication of accomodating water tank (1) lower extreme after filtering.

3. The apparatus for obtaining compatible environmental DNA and microorganisms according to claim 1, wherein: the filtration acquirer is a filter paper type filter membrane, and the filter paper type filter membrane comprises: the filter paper filter comprises filter paper (4-1), a mesh plate (4-2), a hollow filter membrane shell (4-3) and a connecting clamping sleeve (4-4), wherein the mesh plate (4-2) is arranged at the upper end of the hollow filter membrane shell (4-3), the filter paper (4-1) covers the mesh plate (4-2), the connecting clamping sleeve (4-4) is arranged at the upper end of the hollow filter membrane shell (4-3) in a threaded connection manner, a limiting structure of the filter paper (4-1) covering the mesh plate (4-2) is formed between the connecting clamping sleeve (4-4) and the hollow filter membrane shell (4-3), the connecting clamping sleeve (4-4) enters a water inlet structure of a filter acquirer in the hollow filter membrane shell (4-3) through the filter paper (4-1) and the mesh plate (4-2) after water filtration, the lower end opening of the hollow filter membrane shell (4-3) is a water outlet of the filtration acquirer, the other end of the connecting clamping sleeve (4-4) is connected with the water containing filter bowl (5) through threads, and the connecting clamping sleeve (4-4) is communicated with the water containing filter bowl (5) to form a water inlet and water source communicating structure of the filtration acquirer.

4. The apparatus for obtaining compatible environmental DNA and microorganisms according to claim 3, wherein: the water containing filter bowl (5) is provided with volume scales (5-1).