CN216005845U

CN216005845U - Microporous filter membrane string bag for direct dehydration and embedding after cell particles are obtained by centrifugation

Info

Publication number: CN216005845U
Application number: CN202122493679.0U
Authority: CN
Inventors: 秦京生
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-10-18
Filing date: 2021-10-18
Publication date: 2022-03-11
Anticipated expiration: 2031-10-18

Abstract

The utility model relates to a directly dewater and embedded millipore filtration membrane string bag after cell granule is acquireed in centrifugation, its characterized in that: a centrifugation directly dewaters and can embed the microfiltration membrane string bag in paraffin behind obtaining cell granule, comprises the centrifuging tube bottom of the centrifuging tube body on upper portion, bottom and the microfiltration membrane string bag of clamp between centrifuging tube body and centrifuging tube bottom during the use. The utility model discloses a method and device easy operation, the centrifuging tube body on its upper portion, the centrifuging tube bottom of bottom can be assembled each other and dismantle, be convenient for install with take out the clamp can directly carry out cell aggregation granule dehydration and can embed the millipore filtration string bag in paraffin in the centre, optimized the method that the cell that drops in the body fluid collected and simplified the cell aggregation granule dehydration and the embedded procedure of making by a wide margin.

Description

Microporous filter membrane string bag for direct dehydration and embedding after cell particles are obtained by centrifugation

Technical Field

The utility model relates to a how after collecting the cell that drops in the body fluid in centrifugal process, can be with quick simple and convenient with cell aggregation granule dewater and wax dipping until the embedding, carry out subsequent section and dyeing or reagent titration after making cell wax piece and handle, carry out pathological examination.

Background

How to prepare cell wax blocks for cast-off cells in body fluid has many methods at present, and for samples with larger particle size of cell aggregate particles above 2 mm, the cell aggregate particles can be wrapped by filter paper or casing paper, and then dehydrated and waxed until embedded to prepare cell wax blocks; for a sample with the smaller particle size of the cell aggregation particles below 2 mm, the cell aggregation particles can be wrapped by molten carrageenan or agar, and after solidification, dehydration and wax dipping are carried out until embedding is carried out, so as to prepare a cell wax block; the operation process is to take out the cell aggregation particles from the centrifuge tube and wrap the cell aggregation particles, and the method has a plurality of defects for large cell aggregation particles and small cell aggregation particles, wherein the large cell aggregation particles are wrapped by filter paper or an enteric coating, cells become loose or partially run off along with the flowing liquid in the dehydration process, so that not all cell specimens are obtained when cell blocks are embedded; the method comprises the steps of coating the small cell aggregation particles with carrageenan or agar, melting the carrageenan or agar at constant temperature, isolating the cell aggregation particles from contact with filter paper, coating, removing an isolation membrane after solidification, and dehydrating.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects existing in the prior art, the utility model provides a microporous filter membrane string bag which can be directly dehydrated and embedded after cell particles are obtained by centrifugation.

The utility model discloses a directly dewater and embedded millipore filtration membrane string bag after cell granule is acquireed in centrifugation, its characterized in that: the cell particles obtained by centrifugation are directly dehydrated and can

The microfiltration membrane string bag embedded in paraffin comprises a centrifuge tube body on the upper part, a centrifuge tube bottom cover on the bottom part and a microfiltration membrane string bag clamped between the centrifuge tube body and the centrifuge tube bottom cover during use.

Preferably, the centrifuging tube body on the centrifuging tube body that directly dewaters and the embedded millipore filtration string bag upper portion behind the cell granule is acquireed in the centrifugation, the centrifuging tube bottom lid of bottom pass through forms such as buckle, screw thread, grafting or bayonet socket quick convenient mutual dismantlement and connection.

Preferably, the microporous filter membrane string bag which is directly dehydrated and embedded after cell particles are obtained by centrifugation can be closely clamped and attached in a weak glue bonding mode through the overlapping part between the centrifuge tube body at the upper part and the centrifuge tube bottom cover at the bottom.

Preferably, the volume of the sedimentation cavity for cell aggregation at the bottom of the microporous filter membrane net bag is 1-1000 cubic millimeters, and the shape of the sedimentation cavity can be cylindrical, conical, truncated cone, rhombic or polygonal.

Preferably, the thickness of the filter membrane of the microporous filter membrane net bag is 0.1 micron-1 mm.

Preferably, the filter membrane pore size of the microporous filter membrane net bag is 0.1 micron-1 mm.

Preferably, the filter membrane of the microporous filter membrane string bag can be made of materials such as paper, plastics, meridians, silk, collagen, biological gelatin and the like, and when the microporous filter membrane string bag is used for wrapping cell aggregate particles, dehydrating and embedding the cell aggregate particles by paraffin, the microporous filter membrane string bag can be used for slicing by a paraffin slicer.

Adopt the technical scheme of the utility model, can gain following beneficial effect:

1. the utility model discloses technical scheme is because the cavity inlayer of centrifuging tube bottom is the microfiltration membrane string bag, the skin is the cell granule sedimentation chamber of centrifuging tube lower part bottom, both closely laminate, thereby the impact force of producing when the cell granule sedimentation cavity help inlayer of centrifuging tube bottom is accepted and has been resisted the centrifuging tube centrifugation towards the centrifuging tube bottom, the micropore of microfiltration membrane string bag can make liquid flow from the micropore simultaneously, and the cell in the human body fluid sample is intercepted, thereby reach and make the cell gathering in the microfiltration membrane string bag.

2. The technical scheme of the utility model is that after the centrifugation is completed, the microfiltration membrane string bag can be conveniently and quickly taken out, because the microfiltration membrane string bag is provided with micropores, the liquid for dehydrating the cell particles can penetrate through the microfiltration membrane to dehydrate and wax-soak the inside cell aggregate particles, and is embedded in the paraffin together with the cell aggregate particles, so the technical scheme of the utility model saves the procedure of wrapping the cell aggregate particles taken out from the centrifuge tube, and greatly reduces the loss of the cells in the dehydration process; detection errors caused by the binding of the wrappage to the antibody used in the molecular analysis are avoided; saves a plurality of operation links, manpower and material resources.

3. The utility model discloses technical scheme's microfiltration membrane string bag has formed a holistic cell gathering granule in the cavity of string bag accomplishing the centrifugation and with the cell gathering, because the micropore aperture of filter membrane is less than the cell diameter, so can not flow along with mobile liquid and lose in carrying out cell dehydration in-process cell, and the back is accomplished in the dehydration, can also carry out the embedding of cell piece together with the microfiltration membrane string bag, makes the cell wax piece, carries out subsequent section, smear, dyeing or reagent titration processing.

4. The utility model discloses technical scheme simple structure, convenient operation, the person of being convenient for use uses, has solved a large amount of operation links of cell gathering granule in dehydration process, has also avoided the difficult problem of losing of cell at the dehydration in-process simultaneously for can provide the possibility of later stage pathological examination with the cell of whole collections when the centrifugation, improve and diagnose efficiency.

Drawings

FIG. 1 is a schematic cross-sectional view of a microporous membrane string bag for direct dehydration and embedding after cell particles are obtained by centrifugation.

1-centrifuge tube body; 2-centrifuge tube bottom cover; 3-microporous filter membrane net bag; 4-precipitation chamber

Detailed Description

The present invention will be further explained with reference to the following embodiments. Of course, the following detailed description is merely illustrative of various aspects of the invention and should not be taken as limiting the scope of the invention.

Examples

As shown in figure 1, a microporous filter membrane string bag for directly dehydrating and embedding after centrifugally obtaining cell particles comprises a centrifuge tube body 1, a centrifuge tube bottom cover 2, a microporous filter membrane string bag 3 and a precipitation cavity 4; when the microporous filter membrane string bag is used, the proper volume of the cavity of the microporous filter membrane string bag and the proper pore diameter of the microporous filter membrane are selected according to the properties of a specimen, wherein the proper volume is 1-1000 cubic millimeters, and the proper pore diameter is 0.1 micrometer-1 millimeter, so that the specimen is treated.

Example 1

The method for collecting and processing the cells of the body fluid specimen by using the microporous filter membrane string bag which is directly dehydrated and embedded after cell particles are obtained by centrifugation comprises the following steps of;

step 1, the microfiltration membrane string bag is arranged between the centrifuge tube body 1 and the centrifuge tube bottom cover 2, the microfiltration membrane string bag 3 and the centrifuge tube bottom cover 2 are tightly attached to the sedimentation chamber 4, the centrifuge tube body 1 and the centrifuge tube bottom cover 2 are connected in a sealing manner, and the microfiltration membrane string bag 3 is also tightly clamped by the overlapped part to form the whole centrifuge tube.

And 2, adding a body fluid specimen needing to be subjected to exfoliated cell collection from the upper opening of the centrifuge tube body 1.

And 3, centrifuging the tube on a machine to form solid-liquid phase separation.

And 4, taking the tube out of the centrifuge, and pouring out the upper part liquid phase separated in the tube from the upper opening of the centrifuge tube body 1 or sucking out the upper part liquid phase separated in the tube by using a suction pipe.

And 5, opening the connection between the centrifuge tube body 1 and the centrifuge tube bottom cover 2, and taking out the microporous filter membrane string bag 3 in the centrifuge tube bottom cover 2.

And 6, putting the separated solid-phase substance-cell aggregation particles stored in the microfiltration membrane net bag 3 and the microfiltration membrane net bag 3 into an embedding box, and performing subsequent procedures of dehydration, wax dipping, embedding and the like.

The utility model discloses a centrifugation acquires behind the cell granule direct dehydration and embedded millipore filtration string bag, can directly carry out subsequent procedures such as dehydration, waxing, embedding with millipore filtration string bag and wherein the cell gathering granule of collecting after the centrifugation together, no longer need carry out work such as the parcel of cell granule, saved a large amount of manpower and materials for the preparation of cell piece.

The utility model discloses a centrifugation acquires behind the cell granule direct dehydration and embedded millipore filtration string bag, because the design has the millipore filtration string bag, makes cell gathering granule in the procedure such as dehydrating, waxing, embedding, avoided cell gathering granule can not flow along with the liquid that flows and lose, has optimized the cell collection volume by a wide margin, for later stage pathological examination, analysis obtain the improvement of accuracy result, provide probably.

The utility model discloses a direct dehydration and embedded millipore filtration membrane string bag behind cell granule is acquireed in centrifugation, simple structure, convenient operation, convenient to use person uses, improves the treatment effeciency.

Although the present invention has been described and illustrated in detail with reference to specific embodiments thereof, it should be understood that various equivalent changes and modifications can be made therein in accordance with the present invention, and the functional effects thereof are not beyond the spirit of the present invention.

Claims

1. The utility model provides a direct dehydration of cell granule back is acquireed in centrifugation and embedded millipore filtration membrane string bag which characterized in that: a centrifugation directly dewaters and can embed the microfiltration membrane string bag in paraffin behind obtaining cell granule, comprises the centrifuging tube bottom of the centrifuging tube body on upper portion, bottom and the microfiltration membrane string bag of clamp between centrifuging tube body and centrifuging tube bottom during the use.

2. The microporous filter membrane net bag for direct dehydration and embedding after cell particles are obtained by centrifugation as claimed in claim 1, wherein: the centrifuging tube body on upper portion, the centrifuging tube bottom of bottom can be fast convenient mutual dismantlement and connect through the interface to closely press from both sides tight millipore filtration membrane string bag through their overlap.

3. The microporous filter membrane net bag for direct dehydration and embedding after cell particles are obtained by centrifugation as claimed in claim 1, wherein: the bottom of the centrifuge tube bottom cover at the bottom is provided with a sedimentation cavity which can facilitate cell aggregation during centrifugation, and the inner surface of the cavity is tightly attached to the outer surface of the microporous filter membrane net bag.

4. The microporous filter membrane net bag for direct dehydration and embedding after cell particles are obtained by centrifugation as claimed in claim 1, wherein: the volume of a precipitation cavity of the microporous filter membrane net bag, which is convenient for cell precipitation and aggregation, is 1-1000 cubic millimeters.

5. The microporous filter membrane net bag for direct dehydration and embedding after cell particles are obtained by centrifugation as claimed in claim 1, wherein: the thickness of the filter membrane of the microporous filter membrane net bag is 0.1 micron-1 mm.

6. The microporous filter membrane net bag for direct dehydration and embedding after cell particles are obtained by centrifugation as claimed in claim 1, wherein: the filter membrane aperture of the microporous filter membrane net bag capable of collecting the cell aggregation particles is 0.1 micron-1 mm.

7. The microporous filter membrane net bag for direct dehydration and embedding after cell particles are obtained by centrifugation as claimed in claim 1, wherein: the shape of the microporous filter membrane net bag capable of collecting the cell aggregation particles is cylindrical, conical, truncated cone-shaped, rhombic or polygonal with a sealed bottom and an open upper part.

8. The microporous filter membrane net bag for direct dehydration and embedding after cell particles are obtained by centrifugation as claimed in claim 1, wherein: the filter membrane of the microporous filter membrane net bag capable of collecting the cell aggregation particles can be made of paper, plastics, meridians, silk, collagen, biological gelatin and other materials, and can be used for slicing by a paraffin slicer.