CN208748094U - A kind of double gradient centrifuge tube - Google Patents
A kind of double gradient centrifuge tube Download PDFInfo
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- CN208748094U CN208748094U CN201821320017.5U CN201821320017U CN208748094U CN 208748094 U CN208748094 U CN 208748094U CN 201821320017 U CN201821320017 U CN 201821320017U CN 208748094 U CN208748094 U CN 208748094U
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Abstract
The utility model relates to cell extraction techniques field, in particular to a kind of double gradient centrifuge tube, comprising: outer tube, inner tube, liquid storage pot and Guan Gai；Liquid storage pot is fixed on the bottom end of outer tube；Inner tube is arranged in outer tube, and inner tube is matched with outer tube；The bottom end of inner tube is concordant with the upper end of liquid storage pot；Pipe lid is connect with the top of inner tube and outer tube, is used for sealed inner tube and outer tube.The raw material cell liquid for needing to extract is added when inner tube is agreed with outer tube in double gradient centrifuge tube provided by the utility model, and lid upper tube cap is put into centrifuge centrifugation.After the completion of centrifugation, to need the effective cell extracted in liquid storage pot, the above are the gradient liquid abandoned and raw material cell liquid for liquid storage pot.For the gradient liquid and raw material cell liquid abandoned in inner tube, upper lifting pipe lid extracts inner tube out from outer tube, and discarded gradient liquid and former material feed liquid are separated with cell liquid needed for bottom, can high-purity extraction centrifugate bottom cell sample.
Description
Technical field
The utility model relates to cell extraction techniques field, in particular to a kind of double gradient centrifuge tube.
Background technique
Existing cell is centrifuged extractive technique, typically uses single layer centrifuge tube gradient centrifugation, centrifugation is collected after centrifugation
The cell of bottom of the tube.When traditional single layer centrifuge tube cell sample needed for bottom after extracting centrifugation, two methods are generally used:
First method is first to remove sample raw material, gradient centrifugation liquid above with suction pipe, bottom needed for retaining remaining bottom it is thin
Born of the same parents.Second method directly passes through sample raw material, gradient centrifugation liquid with suction pipe and required cell sample is sucked out from centrifugation bottom of the tube.
First method, program is excessively cumbersome, time-consuming to take material；Second method passes through raw material, gradient centrifugation in insertion process
Liquid can more or less pollute required bottom cell component to influence centrifuge results.
Utility model content
The utility model by providing a kind of double gradient centrifuge tube, solve be difficult in the prior art high-purity extract from
The technical issues of heart liquid bottom cell sample.
In order to solve the above technical problems, the utility model embodiment provides a kind of double gradient centrifuge tube, comprising: outer
Pipe, inner tube, liquid storage pot and Guan Gai；The liquid storage pot is fixed on the bottom end of the outer tube；
Said inner tube is arranged in the outer tube, and said inner tube is matched with the outer tube；The bottom end of said inner tube with
The upper end of the liquid storage pot is concordant；
The pipe lid is connect with the top of said inner tube and the outer tube, for sealing said inner tube and the outer tube.
Further, the pipe lid includes: lid ontology, the first sealing ring and the second sealing ring；
The side of the lid ontology is fixed with first sealing ring and second sealing ring, and first sealing ring is set
It sets on the inside of second sealing ring, first sealing ring and second sealing ring constitute concentric circles；The lid ontology
The other side is fixed with the intussusception ring；
First sealing ring is matched with the top of said inner tube, for sealing said inner tube；Second sealing ring
It is matched with the top of the outer tube, for sealing the outer tube.
Further, further includes: outer ring；The outer ring is fixed on outside said inner tube；The outer diameter of the outer ring is greater than institute
The internal diameter of outer tube is stated, the outside of the upper port of the outer tube is arranged in the outer ring.
Further, containing mark line is provided on the liquid storage pot.
Further, the bottom end of the outer tube and the bottom end of said inner tube are conical pipe.
Further, said inner tube and the outer tube are polypropylene material.
Further, the pipe lid is polyethylene material.
One or more technical solutions provided by the utility model, at least have following beneficial effect or advantage:
The former material for needing to extract is added when inner tube is agreed with outer tube in double gradient centrifuge tube provided by the utility model
Expect that cell liquid, lid upper tube cap are put into centrifuge centrifugation.After the completion of centrifugation, the effective cell extracted, liquid storage are needed in liquid storage pot
The above are the gradient liquid abandoned and raw material cell liquid for pot.The gradient liquid and raw material cell liquid abandoned above mention in inner tube
Pipe lid extracts inner tube out from outer tube, and discarded gradient liquid and former material feed liquid are separated with cell liquid needed for bottom, remains in outer tube
The effective cell that remaining underlying liquid as needs to extract, can high-purity extraction centrifugate bottom cell sample.
Detailed description of the invention
Fig. 1 is inner tube structure schematic diagram provided by the embodiment of the utility model；
Fig. 2 is the cross-sectional view of Fig. 1；
Fig. 3 is outer tube structure schematic diagram provided by the embodiment of the utility model；
Fig. 4 is the cross-sectional view of Fig. 2；
Fig. 5 is pipe lid structural schematic diagram provided by the embodiment of the utility model.
Specific embodiment
The utility model embodiment solves by providing a kind of double gradient centrifuge tube and is difficult to high-purity in the prior art
The technical issues of extracting centrifugate bottom cell sample.
Referring to Fig. 1-Fig. 5, the utility model embodiment provides a kind of double gradient centrifuge tube, comprising: outer tube 2, inner tube
3, liquid storage pot 1 and pipe lid 5.Liquid storage pot 1 is fixed on the bottom end of outer tube 2, wherein liquid storage pot 1 is set as semi-spherical shape, liquid storage pot 1
It is integrally formed with outer tube 2 in processing.Inner tube 3 be arranged inside outer tube 2, inner tube 3 is matched with outer tube 2, the outer diameter of inner tube 3 and
The difference in internal diameters control of outer tube 2 is less than 1mm, and the bottom end of inner tube 3 is concordant with the upper end of liquid storage pot 1.Pipe lid 5 and inner tube 3 and outer tube 2
Top connection, is used for sealed inner tube 3 and outer tube 2.
In a specific embodiment provided by the utility model, referring to Fig. 5, pipe lid 5 includes: lid ontology 51, the first sealing ring
53, the second sealing ring 52 and intussusception ring 54.Lid ontology 51 is circular configuration, and the side of Gai Benti 51 is fixed with the first sealing ring
53 and second sealing ring 52, the setting of the first sealing ring 53 is in 52 inside of the second sealing ring, the first sealing ring 53 and the second sealing ring
52 constitute concentric circles.The other side of lid ontology 51 is fixed with intussusception ring 54.First sealing ring 53 is matched with the top of inner tube 3,
For sealed inner tube 3；Second sealing ring 52 is matched with the top of outer tube 2, is used for sealed outer pipe 2；Intussusception ring 54 be used for it is another
52 intussusception of the second sealing ring of one pipe lid 5, two pipe lids 5 are nested together, are conveniently taken.In processing and manufacturing, Gai Benti
51, the first sealing ring 53,52 integral type of the second sealing ring are process.Referring to Fig. 1, Fig. 3 and Fig. 5, pipe lid 5 is can laminated knot
Two pipe lids 5 are nested together by structure when in use, when outer tube 2 and inner tube 3 are agreed with, nestable pipe lid 5 can by outer tube 2 and
Inner tube 3 is covered and is sealed.When above mentioning the pipe lid 5 of intussusception, inner tube 3 can be extracted out from outer tube 2, then separate the pipe of intussusception
Lid 5, a pipe lid 5 of separation, which is placed on outer tube 2, makes its sealing.
In a specific embodiment provided by the utility model, referring to Fig. 3 and Fig. 4, further includes: outer ring 4；Outer ring 4 is fixed on
Outside inner tube 3；The outer diameter of outer ring 4 is greater than the internal diameter of outer tube 2, and the outside of the upper port of outer tube 2 is arranged in outer ring 4.Outer ring 4 is used for
The bottom of limitation inner tube 3 is contacted with the bottom of outer tube 2, guarantees that the bottom end of inner tube 3 is concordant with the upper end of liquid storage pot 1, thin to guarantee
Born of the same parents' extraction effect.
In a specific embodiment provided by the utility model, it is provided with containing mark line on liquid storage pot 1, is extracted for measuring
The volume of object.
In a specific embodiment provided by the utility model, the bottom end of outer tube 2 and the bottom end of inner tube 3 are conical pipe.
In a specific embodiment provided by the utility model, inner tube 3 and outer tube 2 are polypropylene material.
In a specific embodiment provided by the utility model, pipe lid 5 is polyethylene material.
Referring to Fig. 1-Fig. 5, the course of work of double gradient centrifuge tube provided by the utility model is as follows: Step 1: will be interior
Pipe 3 is placed in inside outer tube 2, agrees with inner tube 3 with outer tube 2.Step 2: the raw material cell for needing to extract is added in inner tube 3
Liquid, lid upper tube cap 5 are put into centrifuge centrifugation.Step 3: extracting inner tube 3 out after the completion of being centrifuged, cell and original are extracted required for making
Materials Cell liquid and gradient centrifugation liquid efficiently separate, and need the effective cell extracted in liquid storage pot 1.
One or more technical solutions provided by the utility model, at least have following beneficial effect or advantage:
Referring to Fig. 1-Fig. 5, double gradient centrifuge tube provided by the utility model is added when inner tube 3 is agreed with outer tube 2
The raw material cell liquid for needing to extract, lid upper tube cap 5 are put into centrifuge centrifugation.After the completion of centrifugation, need to mention in liquid storage pot 1
The effective cell taken, the above are the gradient liquid abandoned and raw material cell liquid for liquid storage pot 1.The gradient liquid and raw material cell abandoned
For liquid in inner tube 3, upper lifting pipe lid 5 extracts inner tube 3 out from outer tube 2, and discarded gradient liquid and former material feed liquid are and bottom institute
Cell liquid is needed to separate, the effective cell that remaining underlying liquid as needs to extract in outer tube 2 can high-purity extraction centrifugate bottom
Cell sample.
It should be noted last that the above specific embodiment is only to illustrate the technical solution of the utility model rather than limits
System, although the utility model is described in detail referring to example, those skilled in the art should understand that, it can be right
The technical solution of the utility model is modified or replaced equivalently, without departing from the spirit and model of technical solutions of the utility model
It encloses, should all cover in the scope of the claims of the utility model.
Claims (7)
1. a kind of double gradient centrifuge tube characterized by comprising outer tube, inner tube, liquid storage pot and Guan Gai；The liquid storage pot is solid
It is scheduled on the bottom end of the outer tube；
Said inner tube is arranged in the outer tube, and said inner tube is matched with the outer tube；The bottom end of said inner tube with it is described
The upper end of liquid storage pot is concordant；
The pipe lid is connect with the top of said inner tube and the outer tube, for sealing said inner tube and the outer tube.
2. double gradient centrifuge tube according to claim 1, which is characterized in that the pipe lid includes: lid ontology, first close
Seal ring, the second sealing ring and intussusception ring；
The side of the lid ontology is fixed with first sealing ring and second sealing ring, and the first sealing ring setting exists
On the inside of second sealing ring, first sealing ring and second sealing ring constitute concentric circles；The lid ontology it is another
Side is fixed with the intussusception ring；
First sealing ring is matched with the top of said inner tube, for sealing said inner tube；Second sealing ring and institute
The top for stating outer tube matches, for sealing the outer tube.
3. double gradient centrifuge tube according to claim 1 or 2, which is characterized in that further include: outer ring；The outer ring is solid
It is scheduled on outside said inner tube；The outer diameter of the outer ring is greater than the internal diameter of the outer tube, and the upper of the outer tube is arranged in the outer ring
The outside of port.
4. double gradient centrifuge tube according to claim 1, which is characterized in that be provided with containing mark on the liquid storage pot
Line.
5. double gradient centrifuge tube according to claim 1, which is characterized in that the bottom end of the outer tube and said inner tube
Bottom end is conical pipe.
6. double gradient centrifuge tube according to claim 1 or 2, which is characterized in that said inner tube and the outer tube are poly-
Propylene material.
7. double gradient centrifuge tube according to claim 1 or 2, which is characterized in that the pipe lid is polyethylene material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821320017.5U CN208748094U (en) | 2018-08-13 | 2018-08-13 | A kind of double gradient centrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821320017.5U CN208748094U (en) | 2018-08-13 | 2018-08-13 | A kind of double gradient centrifuge tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208748094U true CN208748094U (en) | 2019-04-16 |
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ID=66080299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821320017.5U Active CN208748094U (en) | 2018-08-13 | 2018-08-13 | A kind of double gradient centrifuge tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208748094U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114719946A (en) * | 2022-04-20 | 2022-07-08 | 东北农业大学 | Device and method for weighing fresh weight and dry weight of cells |
-
2018
- 2018-08-13 CN CN201821320017.5U patent/CN208748094U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114719946A (en) * | 2022-04-20 | 2022-07-08 | 东北农业大学 | Device and method for weighing fresh weight and dry weight of cells |
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