CN114719946A - Device and method for weighing fresh weight and dry weight of cells - Google Patents
Device and method for weighing fresh weight and dry weight of cells Download PDFInfo
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- CN114719946A CN114719946A CN202210415497.8A CN202210415497A CN114719946A CN 114719946 A CN114719946 A CN 114719946A CN 202210415497 A CN202210415497 A CN 202210415497A CN 114719946 A CN114719946 A CN 114719946A
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- 238000005303 weighing Methods 0.000 title claims abstract description 178
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000007789 sealing Methods 0.000 claims abstract description 63
- 239000000463 material Substances 0.000 claims abstract description 18
- 238000001035 drying Methods 0.000 claims abstract description 10
- 238000009423 ventilation Methods 0.000 claims description 33
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 239000000741 silica gel Substances 0.000 claims description 8
- 229910002027 silica gel Inorganic materials 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 47
- 239000004677 Nylon Substances 0.000 description 5
- 229920001778 nylon Polymers 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 229920002379 silicone rubber Polymers 0.000 description 3
- 239000004945 silicone rubber Substances 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01G—WEIGHING
- G01G17/00—Apparatus for or methods of weighing material of special form or property
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01G—WEIGHING
- G01G21/00—Details of weighing apparatus
- G01G21/22—Weigh pans or other weighing receptacles; Weighing platforms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01G—WEIGHING
- G01G21/00—Details of weighing apparatus
- G01G21/28—Frames, Housings
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a device and a method for weighing fresh and dry cell weights, which belong to the technical field of cell engineering and aim to solve the problems of larger error, inaccurate result and more operation steps of the traditional cell weighing method, the device for weighing fresh and dry cell weights comprises an outer weighing tube, an inner weighing tube, a sealing cover, a connecting band and a detachable filter tube, wherein the outer weighing tube and the inner weighing tube can be independently used, the inner weighing tube is used for weighing, the outer tube is used for centrifuging, the inner tube only stores substances to be weighed after centrifuging, the outer tube is a culture carrier, the detachable filter tube is arranged at the bottom of the inner weighing tube, the detachable filter tubes with different specifications can be selected according to the weighed materials, and only the detachable filter tubes need to be discarded after the weighing tube is used, the outer tube and the inner tube of the weighing tube are reserved, the waste is reduced, the energy is saved, the environment is protected, and the weighing tube only needs to be weighed, centrifuged and weighed in the fresh weight weighing test process; in the process of weighing the dry weight, only one-step drying is needed on the basis of the fresh weight.
Description
Technical Field
The invention belongs to the technical field of cell engineering, and particularly relates to a device and a method for weighing fresh weight and dry weight of cells.
Background
Fresh Cell Weight (FCW), which refers to the weight of a cell in its living state, including water; dry Cell Weight (DCW) refers to the dry weight of cells after removal of water. The fresh and dry cell weights of unicellular and multicellular organisms are important indicators for studying cell proliferation, which reflect important characteristics of cell growth, development, proliferation, metabolism, and the like. Such activities of cells maintain the normal operation of a living body, and therefore, accurate weighing of the fresh weight and the dry weight of the cells is an important basis for grasping the proliferation and growth, metabolites, nutrition and water absorption conditions of the cultured cells.
The traditional method for measuring the fresh weight of the cells comprises the steps of weighing filter paper and weighing nylon net by vacuum filtration.
(1) The filter paper weighing method is suitable for cell materials cultured in agar solid, and specifically comprises the steps of taking out the cell materials cultured in agar, washing off the agar, absorbing water by using filter paper, and weighing the weight of the cell materials;
(2) the nylon net weighing method is suitable for suspended cell materials cultured in liquid, the cultured cell materials are placed on a nylon net with known weight for filtration, the residual culture medium of the nylon net is washed, then the water is removed by vacuum filtration, and finally the fresh weight of the cells is obtained by weighing.
The traditional method for measuring the dry cell weight is based on the fresh weight method, the fresh weight cells are transferred from a filter paper or a nylon net and transferred into a constant temperature drying oven to be dried to be constant weight, and finally the dry cell weight is obtained by weighing.
Although the fresh and dry cell weights can be weighed to a certain degree by the traditional weighing method, the problems of poor filter paper adsorption effect, insufficient elution, incomplete vacuum filtration dewatering and the like can occur in actual operation, so that the error is large, and the weighing result is inaccurate. Therefore, the development of an accurate, simple and rapid device for weighing the fresh and dry cell weight and a weighing method thereof are in line with practical needs.
Disclosure of Invention
The invention provides a device and a method for weighing fresh and dry cell weights, which aim to solve the problems of large error, inaccurate result and multiple operation steps of the traditional cell weighing method;
a device for weighing the fresh weight and the dry weight of cells comprises a weighing outer tube, a weighing inner tube, a sealing cover, a connecting band and a detachable filter tube;
the detachable filter tube is arranged at the bottom of the weighing inner tube and is coaxially arranged with the weighing inner tube, the detachable filter tube is detachably connected with the weighing inner tube, the whole body formed by the weighing inner tube and the detachable filter tube is inserted on the weighing outer tube, the weighing inner tube and the weighing outer tube are arranged in a lap joint manner, the sealing cover is inserted at a feed inlet of the weighing inner tube, a connecting band is arranged between the sealing cover and the weighing inner tube, one end of the connecting band is fixedly connected with the sealing cover, and the other end of the connecting band is fixedly connected with the weighing inner tube;
furthermore, the top of the weighing outer tube is an open end, the bottom of the weighing outer tube is a closed end, a lap joint outer ring is sleeved on the outer circular surface of the open end, the lap joint outer ring and the weighing outer tube are integrally formed, and the top surface of the lap joint outer ring and the top surface of the weighing outer tube are positioned on the same plane;
furthermore, the weighing inner tube comprises a middle tube body, an overlap joint tube body, a conical tube body and a connecting tube, the overlap joint tube body, the middle tube body, the conical tube body and the connecting tube are coaxially and sequentially arranged from top to bottom, the bottom end of the overlap joint tube body and the top end of the middle tube body are integrally formed to form a stepped tube body, the bottom end of the middle tube body and the large-opening end of the conical tube body are integrally formed, the small-opening end of the conical tube body and the top end of the connecting tube are integrally formed, internal threads are machined on the inner wall of the connecting tube, and the step between the overlap joint tube body and the middle tube body is overlapped on an overlap joint outer ring at the opening end of the weighing outer tube;
furthermore, the sealing cover comprises a cover body, a sealing pipe and a sealing ring, the sealing pipe is arranged on the lower surface of the sealing cover, the top end of the sealing pipe is fixedly connected with the lower surface of the cover body, the sealing ring is arranged on the inner circular surface of the bottom end of the sealing pipe, the sealing ring and the sealing pipe are coaxially arranged, the bottom end surface of the sealing ring and the bottom end surface of the sealing pipe are arranged in a coplanar manner, the outer circular surface of the sealing ring is fixedly connected with the inner wall of the bottom end of the sealing pipe, one end of the connecting belt is fixedly connected with the outer side wall of the cover body, and the other end of the connecting belt is fixedly connected with the outer wall of the top end of the lap joint pipe body;
furthermore, a ventilation groove is processed in the center of the upper surface of the cover body, a ventilation area is arranged in the center of the bottom of the ventilation groove, a plurality of ventilation through holes are processed in the ventilation area, N sealing holes are processed in the bottom of the ventilation groove at equal intervals along the circumferential direction, N is a positive integer, the N sealing holes are all arranged around the ventilation area, a ventilation plug is arranged in the ventilation groove, N silica gel soft plugs are arranged at the bottom end of the ventilation plug along the circumferential direction, each silica gel soft plug is arranged corresponding to one sealing hole, and the N silica gel soft plugs on the ventilation plug are correspondingly inserted into the N sealing holes;
furthermore, the detachable filter tube comprises a tube body, a filter screen and an external threaded tube, the external threaded tube is arranged at the top end of the tube body, the tube body and the external threaded tube are coaxially arranged, the bottom end of the external threaded tube and the top end of the tube body are integrally formed, the filter screen is arranged on the inner wall of the tube body and is close to the bottom end of the tube body, the filter screen is fixedly connected with the inner wall of the tube body, external threads on the outer circular surface of the external threaded tube are matched with internal threads on the inner wall of the connecting tube, and the detachable filter tube is detachably connected with the connecting tube through the external threaded tube;
furthermore, the device also comprises a base, wherein a caulking groove is processed on the top surface of the base, the inner contour of the caulking groove is the same as the outer contour of the bottom of the weighing outer tube, and the weighing outer tube is inserted on the base;
a method for weighing the fresh weight and the dry weight of cells is realized by the following steps:
the method comprises the following steps: weighing the weight of the inner pipe when the inner pipe is unloaded; matching the detachable filter tube which is in accordance with the aperture of the plant cell to be weighed with the weighing inner tube, placing the detachable filter tube on an electronic balance for weighing to obtain the weight W1；
Step two: centrifuging the cells; adding a fixed volume of cell material into the inner tube of the weighing tube by using a pipette, placing the weighing inner tube into the weighing outer tube, and transferring the weighing inner tube into a centrifuge for centrifugation, wherein the centrifuge is set at a rotation speed of 1000-;
step three: weighing the weight of the inner tube when weighing the object; separating the weighing inner tube from the weighing outer tube after centrifugation, wherein the culture solution for culturing cells in the weighing outer tube is discarded; weighing the cell material to be weighed in the inner tube, putting the inner tube on an electronic balance again for weighing to obtain the weight W2At this time, the fresh weight of the cells is W2-W1；
Step four: weighing the dried inner tube when carrying the object; the weighing inner tube is arranged on the weighing outer tube and is just placed in a constant temperature drying box at 65 ℃. The ventilation plug above the sealing cover is opened, and the ventilation through hole below the sealing cover is exposed to strengthen air circulation, so that the cell material can be rapidly dried. Drying at constant temperature until the weight of the weighing inner tube is not changed any more, and recording the weight W3. At this time, the dry cell weight is W3-W1；
Step five: after the weighing work is finished, only the detachable filter tube needs to be discarded, and the outer tube and the weighing inner tube can be reused after being cleaned and weighed.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the device for weighing the fresh weight and the dry weight of the cells, the weighing tube is used for distinguishing the inner tube and the outer tube which can be used independently, the inner tube is used for weighing, the outer tube is used for centrifuging, only the substances to be weighed are stored in the inner tube after centrifuging, and the outer tube is used as a culture carrier. Compared with the traditional weighing method, the method reduces the human and test errors and ensures that the result is more accurate.
2. According to the device for weighing the fresh weight and the dry weight of the cells, the detachable filter tubes are arranged at the bottom of the weighing inner tube, the detachable filter tubes with different specifications can be selected according to the weighed substances, the detachable filter tubes are only required to be discarded after the use is finished, the outer tube and the inner tube of the weighing tube are reserved, the waste is reduced, and the device is energy-saving and environment-friendly.
3. According to the device for weighing the fresh weight and the dry weight of the cells, the weighing tube only needs to carry out weighing-centrifugation-weighing in the test process of weighing the fresh weight; in the process of weighing the dry weight, only one-step drying is needed on the basis of the fresh weight, so that the whole operation flow is simplified, and the efficiency is improved;
4. the method for weighing the fresh weight and the dry weight of the cells is simple to operate, low in cost, accurate in weighing value and suitable for popularization and use.
Drawings
FIG. 1 is a schematic main sectional view of the present invention:
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is a schematic main sectional view of the weighing inner tube according to the present invention;
FIG. 4 is a schematic front view of the weighing inner tube of the present invention;
FIG. 5 is a schematic main sectional view of the sealing cover of the present invention;
FIG. 6 is a side view of the sealing cap of the present invention;
FIG. 7 is a schematic view of the sealing cap of the present invention in the direction A-A;
FIG. 8 is a schematic main sectional view of a removable filter tube according to the present invention;
FIG. 9 is a bottom view of a removable filter tube according to the present invention;
in the figure, 1 weighs outer tube, 2 weighs inner tube, 21 middle tube, 22 lap tube, 23 taper tube, 24 connecting tube, 3 sealing cover, 31 cover, 32 sealing tube, 33 ventilating groove, 34 ventilating through hole, 35 ventilating plug, 36 silica gel soft plug, 37 sealing ring, 4 connecting band, 5 detachable filter tube, 51 tube, 52 filter screen, 53 external thread tube and 6 base.
Detailed Description
The first embodiment is as follows: the present embodiment will be described with reference to fig. 1 to 9, and provides an apparatus for weighing the fresh weight and dry weight of cells, characterized in that: the device comprises a weighing outer pipe 1, a weighing inner pipe 2, a sealing cover 3, a connecting belt 4 and a detachable filter pipe 5;
can dismantle the bottom that filter tube 5 set up weighing inner tube 2, and can dismantle filter tube 5 and weigh 2 coaxial settings of inner tube, can dismantle filter tube 5 and weigh inner tube 2 and dismantle and be connected, weigh inner tube 2 and can dismantle the whole of filter tube 5 constitution and insert and establish on weighing outer tube 1, and weigh inner tube 2 and weigh 1 overlap joint settings of outer tube, sealed lid 3 is inserted and is established in the feed inlet department of weighing inner tube 2, sealed lid 3 with weigh and be equipped with connecting band 4 between the inner tube 2, the one end and the sealed 3 fixed connection of connecting band 4, the other end of connecting band 4 with weigh 2 fixed connection of inner tube.
In this embodiment, the weighing outer tube 1, the weighing inner tube 2, the sealing cover 3, the connecting band 4 and the detachable filter tube 5 are all made of polypropylene materials.
The second embodiment is as follows: the present embodiment is described with reference to fig. 1 to 9, and is further limited to the weighing outer tube 1 according to the first embodiment, in the present embodiment, the top of the weighing outer tube 1 is an open end, the bottom of the weighing outer tube 1 is a closed end, an overlapping outer ring is sleeved on an outer circumferential surface of the open end, the overlapping outer ring and the weighing outer tube 1 are integrally formed, and other components and connection manners of the top surface of the overlapping outer ring and the top surface of the weighing outer tube are located on the same plane are the same as those of the first embodiment.
In this embodiment, weigh 1 outer wall of outer tube and be carved with the scale mark, weigh 1 external diameter of outer tube and centrifuge tube trench phase-match, the diameter of weighing 1 upper portion overlap joint outer lane of outer tube is wider in centrifuge tube trench for the weighing tube can be fixed in on the centrifuge tube trench.
The third concrete implementation mode: referring to fig. 1 to 6, this embodiment is described, and the weighing inner tube 2 according to the second embodiment is further defined, in this embodiment, the weighing inner tube 2 includes a middle tube body 21, an overlapping tube body 22, a tapered tube body 23, and a connecting tube 24, the overlapping tube body 22, the middle tube body 21, the tapered tube body 23, and the connecting tube 24 are coaxially and sequentially disposed from top to bottom, a bottom end of the overlapping tube body 22 and a top end of the middle tube body 21 are integrally formed to form a stepped tube body, a bottom end of the middle tube body 21 and a large-opening end of the tapered tube body 23 are integrally formed, a small-opening end of the tapered tube body 23 and a top end of the connecting tube 24 are integrally formed to form a stepped tube body, an internal thread is processed on an inner wall of the connecting tube 24, and a step between the overlapping tube body 22 and the middle tube body 21 is overlapped on an overlapping outer ring of an opening end of the weighing outer tube 1. Other components and connection modes are the same as those of the first embodiment.
In the present embodiment, the weighing inner tube 2 functions to contain a weighed material. The diameter of the overlapping tube body 22 at the upper part of the weighing inner tube 2 is slightly larger than that of the middle tube body 21, so that the weighing inner tube is suspended in the weighing outer tube.
The fourth concrete implementation mode: the present embodiment is described with reference to fig. 1 to 9, and is further defined by the sealing cover 3 according to the third embodiment, in which the sealing cover 3 includes a cover body 31, a sealing tube 32 and a sealing ring 37, the sealing tube 32 is disposed on the lower surface of the sealing cover 3, the top end of the sealing tube 32 is fixedly connected to the lower surface of the cover body 31, the sealing ring 37 is disposed on the inner circumferential surface of the bottom end of the sealing tube 32, the sealing ring 37 is disposed coaxially with the sealing tube 32, the bottom end surface of the sealing ring 37 is disposed coplanar with the bottom end surface of the sealing tube 32, the outer circumferential surface of the sealing ring 37 is fixedly connected to the inner wall of the bottom end of the sealing tube 32, one end of the connecting band 4 is fixedly connected to the outer sidewall of the cover body 31, and the other end of the connecting band 4 is fixedly connected to the outer wall of the top end of the bridging tube body 22. Other components and connection modes are the same as those of the third embodiment.
In this embodiment, a sealing tube 32 is provided under the sealing cover 3, the sealing tube 32 is made of rubber, and a ring of sealing ring 37 is provided on the inner wall of the sealing tube 32, and cooperates with an inner step formed by the middle tube body 21 and the lap tube body 22 in the weighing inner tube 2 to seal the weighing inner tube.
The fifth concrete implementation mode: the present embodiment is described with reference to fig. 1 to 9, and is further limited to the cap 31 described in the fourth embodiment, in the present embodiment, a ventilation groove 33 is formed in the center of the upper surface of the cap 31, a ventilation area is formed in the center of the bottom of the ventilation groove 33, a plurality of ventilation through holes 34 are formed in the ventilation area, N sealing holes are formed in the bottom of the ventilation groove 33 at equal intervals along the circumferential direction, N is a positive integer, the N sealing holes are all arranged around the ventilation area, a ventilation plug 35 is arranged in the ventilation groove 33, N silicone rubber plugs 36 are arranged at the bottom end of the ventilation plug 35 along the circumferential direction, each silicone rubber plug 36 is arranged corresponding to one sealing hole, and the N silicone rubber plugs 36 on the ventilation plug 35 are correspondingly inserted into the N sealing holes. The other components and the connection mode are the same as those of the fourth embodiment.
In this embodiment, a ventilation groove 33 is formed in the cover body 31, a ventilation plug 35 is arranged in the ventilation groove 33, N soft silica gel plugs 36 are connected to the lower side of the ventilation plug 35 and embedded in the cover body 31, a plurality of ventilation through holes 34 are formed in the lower side of the ventilation plug 35 to achieve ventilation, and the value range of N is generally 2-4.
The sixth specific implementation mode: the present embodiment is described with reference to fig. 1 to 9, and the present embodiment is further limited to the detachable filter tube 5 described in the fifth embodiment, in the present embodiment, the detachable filter tube 5 includes a tube body 51, a filter screen 52 and an external threaded tube 53, the external threaded tube 53 is disposed at the top end of the tube body 51, the tube body 51 and the external threaded tube 53 are coaxially disposed, the bottom end of the external threaded tube 53 and the top end of the tube body 51 are integrally formed, the filter screen 52 is disposed on the inner wall of the tube body 51, the filter screen 52 is disposed near the bottom end of the tube body 51, the filter screen 52 is fixedly connected to the inner wall of the tube body 51, the external thread on the outer circumferential surface of the external threaded tube 53 is disposed in cooperation with the internal thread on the inner wall of the connection tube 24, and the detachable filter tube 5 is detachably connected to the connection tube 24 through the external threaded tube 53. The other components and the connection mode are the same as those of the fourth embodiment.
In the embodiment, the detachable filter tube 5 is connected with the weighing inner tube 2 in a threaded fastening mode, the filter screen 52 is arranged in the detachable filter tube 5, the filter tube can be conveniently detached and replaced according to the weighed substances, and the filter screens 52 with different apertures correspondingly play a role in filtering different weighed substances; a fixed ring can be arranged below the inner wall of the detachable filter tube 5, and the diameter of the outer wall of the fixed ring is equivalent to that of the inner wall of the detachable filter tube; the bottom of the inner wall of the detachable filter tube is provided with an anti-slip ring, so that the fixed ring is prevented from falling off in the centrifugal process; the fixed ring and the anti-slip ring act together to tightly press the filter screen 52 in the detachable filter tube, and the filter screen 2 is made of cellulose and polysulfone materials.
The seventh embodiment: the present embodiment is described with reference to fig. 1 to 9, and is further limited to the device according to the sixth embodiment, and the device according to the present embodiment further includes a base 6, a caulking groove is formed on the top surface of the base 6, the inner contour of the caulking groove is the same as the outer contour of the bottom of the weighing outer tube 1, and the weighing outer tube 1 is inserted into the base 6. Other components and connection modes are the same as those of the sixth embodiment.
So set up, weigh the outer tube 1 and mainly play and hold and weigh the inner tube 2 and filtrate the solution from the inner tube role; the base is arranged to support the outer tube of the weighing tube to be vertically placed.
The specific implementation mode eight: the present embodiment is described with reference to fig. 1 to 9, and provides a method for weighing the fresh weight and dry weight of cells, which is realized by the following steps:
the method comprises the following steps: weighing the weight of the inner pipe when the inner pipe is unloaded; matching the detachable filter tube 5 which is in accordance with the aperture of the plant cell to be weighed with the weighing inner tube 2, placing the filter tube on an electronic balance for weighing to obtain the weight W1；
Step two: centrifuging the cells; adding a fixed volume of cell material into the inner tube of the weighing tube by using a pipette, placing the weighing inner tube 2 into the weighing outer tube 1 and transferring the weighing outer tube to a centrifuge for centrifugation, wherein the centrifuge is set at a rotation speed of 1000-;
step three: weighing the weight of the inner tube when weighing the object; after centrifugation, separating the weighing inner tube 2 from the weighing outer tube 1, wherein the culture solution for culturing cells in the weighing outer tube 1 is discarded; weighing the cell material to be weighed in the inner tube 2, putting the inner tube 2 on the electronic balance again, and weighing to obtain the weight W2At this time, the fresh weight of the cells is W2-W1；
Step four: weighing the dried inner tube when carrying the object; the weighing inner tube 2 is arranged on the weighing outer tube 1 and is just placed in a constant temperature drying box at 65 ℃. The air-permeable plug 35 above the sealing cover 3 is opened to expose the air-permeable through hole 34 below to enhance air circulation, which is beneficial to rapid drying of cell materials. Drying at constant temperature until the weight of the weighing inner tube 2 does not change any more, and recording the weight W3. At this time, the dry cell weight is W3-W1；
Step five: after the weighing work is finished, only the detachable filter tube 5 needs to be discarded, and the outer weighing tube 1 and the inner weighing tube 2 can be reused after being cleaned.
The present invention is not limited to the above embodiments, and any person skilled in the art can make many modifications and equivalent variations by using the above-described structures and technical contents without departing from the scope of the present invention.
Claims (8)
1. An apparatus for weighing fresh weight and dry weight of cells is characterized in that: the device comprises a weighing outer pipe (1), a weighing inner pipe (2), a sealing cover (3), a connecting belt (4) and a detachable filter pipe (5);
can dismantle chimney filter (5) set up in the bottom of weighing inner tube (2), and can dismantle chimney filter (5) and weigh inner tube (2) coaxial setting, can dismantle chimney filter (5) and weigh inner tube (2) and dismantle and be connected, weigh inner tube (2) and can dismantle the whole of chimney filter (5) constitution and insert and establish on weighing outer tube (1), and weigh inner tube (2) and weigh outer tube (1) overlap joint setting, sealed lid (3) are inserted and are established at the feed inlet department of weighing inner tube (2), sealed lid (3) and weigh and be equipped with connecting band (4) between inner tube (2), the one end and sealed lid (3) fixed connection of connecting band (4), the other end of connecting band (4) and weigh inner tube (2) fixed connection.
2. The device for measuring the fresh weight and the dry weight of the cells according to claim 1, wherein: the top of weighing outer tube (1) is the open end, and the bottom of weighing outer tube (1) is the blind end, and the cover is equipped with the overlap joint outer lane on the excircle face of open end, and the overlap joint outer lane sets up with weighing outer tube (1) integrated into one piece, and the top surface of overlap joint outer lane and the top surface of weighing the outer tube are located the coplanar.
3. The apparatus according to claim 2, wherein the dry weight of the fresh weight of the cells is: weigh inner tube (2) including middle body (21), overlap joint body (22), toper body (23) and connecting pipe (24), overlap joint body (22), middle body (21), toper body (23) and connecting pipe (24) from top to bottom coaxial set gradually, and the bottom of overlap joint body (22) sets up with the top integrated into one piece of middle body (21) and forms the ladder body, the bottom of middle body (21) and the macrostoma end integrated into one piece setting of toper body (23), the macrostoma end of toper body (23) sets up with the top integrated into one piece of connecting pipe (24), processing has the internal thread on the inner wall of connecting pipe (24), step overlap joint between overlap joint body (22) and middle body (21) is on weighing the overlap joint outer lane of outer tube (1) open end.
4. The apparatus according to claim 3, wherein the dry weight of fresh cell weight is measured by: sealed lid (3) are including lid (31), sealed tube (32) and sealing ring (37), sealed tube (32) set up on the lower surface of sealed lid (3), and the top of sealed tube (32) and the lower fixed surface of lid (31) are connected, sealing ring (37) set up on the interior disc of sealed tube (32) bottom, and sealing ring (37) and sealed tube (32) coaxial setting, the bottom terminal surface of sealed tube (37) and the bottom terminal surface coplane setting of sealed tube (32), the outer disc of sealed ring (37) and the inner wall fixed connection of sealed tube (32) bottom, the one end of connecting band (4) and the lateral wall fixed connection of lid (31), the other end and the overlap joint body (22) top outer wall fixed connection of connecting band (4).
5. The apparatus according to claim 4, wherein the dry weight of fresh cell weight is measured by: the processing of the center department of lid (31) upper surface has ventilative recess (33), the center department of ventilative recess (33) bottom is equipped with ventilative district, processing has a plurality of ventilative through-holes (34) in the ventilative district, the bottom of ventilative recess (33) has a N sealed hole along circumference equidistance processing, N is positive integer, a N sealed hole all sets up around ventilative district, be equipped with ventilative stopper (35) in ventilative recess (33), the bottom of ventilative stopper (35) is equipped with a N soft stopper of silica gel (36) along circumference, every soft stopper of silica gel (36) corresponds the setting with a sealed hole, a N soft stopper of silica gel (36) on ventilative stopper (35) correspond the cartridge on a N sealed hole.
6. The apparatus according to claim 5, wherein the dry weight of the fresh weight of the cells is: can dismantle filter tube (5) including body (51), filter screen (52) and external screw thread pipe (53), external screw thread pipe (53) set up the top at body (51), and body (51) and external screw thread pipe (53) coaxial setting, the bottom of external screw thread pipe (53) sets up with the top integrated into one piece of body (51), filter screen (52) set up on the inner wall of body (51), and filter screen (52) are close to the bottom setting of body (51), the inner wall fixed connection of filter screen (52) and body (51), the external screw thread on external circular surface of external screw thread pipe (53) sets up with the interior screw-thread fit of connecting pipe (24) inner wall, can dismantle filter tube (5) through external screw thread pipe (53) and connecting pipe (24) threaded disassembly and be connected.
7. The apparatus according to claim 6, wherein the dry weight of fresh cell weight is: the device also comprises a base (6), a caulking groove is processed on the top surface of the base (6), the inner contour of the caulking groove is the same as the outer contour of the bottom of the weighing outer tube (1), and the weighing outer tube (1) is inserted on the base (6).
8. A weighing method using the apparatus for weighing fresh and dry cell weights according to any one of claims 1 to 7, wherein: the method is realized by the following steps:
the method comprises the following steps: weighing the weight of the inner pipe when the inner pipe is unloaded; matching a detachable filter tube (5) which accords with the size and the aperture of the weighed plant cells to the weighing inner tube (2), placing the detachable filter tube on an electronic balance for weighing, and obtaining the weight W1；
Step two: centrifuging the cells; adding a fixed volume of cell material into the inner tube of the weighing tube by using a pipette, placing the weighing inner tube (2) into the weighing outer tube (1) and transferring the weighing inner tube into a centrifuge for centrifugation, wherein the centrifuge is set at a rotation speed of 1000-;
step three: weighing the weight of the inner tube when weighing the object; after centrifugation, separating the weighing inner tube (2) from the weighing outer tube (1), wherein the culture solution for culturing cells in the weighing outer tube (1) is discarded; weighing the cell material to be weighed in the inner tube (2), putting the inner tube (2) on the electronic balance again, and weighing to obtain the weight W2At this time, the fresh weight of the cells is W2-W1；
Step four: weighing the dried inner tube when carrying the object; placing the weighing inner tube (2) on the weighing outer tube (1), and placing the weighing inner tube and the weighing outer tube at a constant temperature of 65 DEG CAnd (4) drying in the oven. The upper ventilation plug (35) of the sealing cover (3) is opened, and the lower ventilation through hole (34) is exposed to enhance air circulation, so that the cell material can be dried quickly. Drying at constant temperature until the weight of the weighing inner tube (2) is not changed any more, and recording the weight W3. At this time, the dry cell weight is W3-W1；
Step five: after the weighing work is finished, only the detachable filter tube (5) is needed to be discarded, and the outer weighing tube (1) and the inner weighing tube (2) can be cleaned and reused.
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