CN212087676U

CN212087676U - Disposable pine wood nematode collecting and separating device

Info

Publication number: CN212087676U
Application number: CN201922335155.1U
Authority: CN
Inventors: 黄华艳; 赵鹏飞; 蒋学建; 赵程劼; 秦庭伟; 常明山
Original assignee: Guangxi Zhuang Autonomous Region Forestry Research Institute
Current assignee: Guangxi Zhuang Autonomous Region Forestry Research Institute
Priority date: 2019-12-24
Filing date: 2019-12-24
Publication date: 2020-12-08
Anticipated expiration: 2029-12-24

Abstract

The utility model discloses a separation device for collecting pine wood nematodes at one time, which is provided with a collecting bottle, a bottle cap, a funnel A, filter paper and a funnel B; a funnel A is arranged in a collecting bottle opening of the collecting bottle, and filter paper and a funnel B are sequentially arranged in the funnel A; the bottle cap is internally provided with a bottle cap convex ring, the bottle cap is in threaded fit connection with a collecting bottle opening of a collecting bottle, the funnel wall of the funnel A at one end of the funnel opening is uniformly provided with 1-3 circles of filtering holes, the funnel wall and the funnel bottom are connected into a whole, the funnel opening of the funnel A is provided with a vertical convex edge or a flat convex edge, the vertical convex edge is matched with the inner diameter of the collecting bottle opening, and the outer diameter of the flat convex edge is matched with the outer diameter of the collecting bottle opening. The filter separation of this separator and the effectual of collecting pine wood nematode, the simple operation, small easily carries, and when field operation, can the quickly separating out pine wood nematode, the reliability of ensureing the result is fit for middle-size and small-size forest defence station etc..

Description

Disposable pine wood nematode collecting and separating device

Technical Field

The utility model relates to a biological control technique relates to pine wood nematode separation and collects technical field, specifically is a disposable pine wood nematode separator that collects that is used for filtering separation and collects pine wood nematode sample.

Background

Pine wood nematode is the cause of pine wood nematode epidemics, and diseased pine wood will die rapidly, have high transmissibility and cause a large number of pine trees to die in a short time, and is called cancer of pine trees. Therefore, rapid quarantine of pine wood nematodes is an important prevention means. At present, the related quarantine departments commonly adopt indoor temporary inspection and a Bellman funnel method to separate the pine wood nematodes in laboratories. A large number of funnel devices and special fields are needed during batch detection, and the operation problem can be encountered due to the complex structure.

The existing separation device can not be operated in the field, the operation is complex, each single filter device needs to be specially cleaned, and the sample can be cross-infected due to incomplete cleaning. In view of the above, the utility model aims at providing an use portable once only to collect pine wood nematode separator.

Disclosure of Invention

An object of the utility model is to provide a convenient practicality, economy are cheap, can separate the nematode in open-air everywhere sample, and pine wood nematode separator is once only collected to prevention sample cross contamination possibility.

In order to achieve the above object, the present invention provides the following technical solutions:

a disposable pine wood nematode collecting and separating device is provided with a collecting bottle, a collecting bottle opening, a bottle cap, a funnel A, filter paper and a funnel B; a funnel A is arranged in a collecting bottle opening of the collecting bottle, and filter paper and a funnel B are sequentially arranged in the funnel A; a bottle cap convex ring is arranged in the bottle cap, and the bottle cap is in threaded fit connection with a collecting bottle opening of the collecting bottle.

Furthermore, the funnel wall of the funnel A at one end of the funnel opening is uniformly provided with a plurality of 1-3 circles of filtering holes, the funnel wall and the funnel bottom are connected into a whole, the funnel opening of the funnel A is provided with a funnel convex edge, the funnel convex edge is set to be a vertical convex edge or a flat convex edge, the vertical convex edge of the funnel A with the vertical convex edge is matched with the inner aperture of the collecting bottle opening, and the outer diameter of the flat convex edge of the funnel A with the flat convex edge is matched with the outer aperture of the collecting bottle opening.

Further, the outer diameter of the collecting bottle mouth is 25-30 mm.

Further, the diameter of the filtering hole is 1-2 mm.

Further, the funnel A is formed by injection molding or is formed by pressing and molding degradable composite materials.

Further, the depth of the funnel A is 7-10 mm.

Further, the collecting bottle and the bottle cap are commercially available small-sized mineral water bottles.

Further, the setting of funnel B is the same with funnel A's setting, and the setting of filter paper is different with funnel A and funnel B's setting for there is not the filtration pore.

Further, the height of the bottle cap convex ring is 3-5 mm.

The utility model discloses a have following several kinds of beneficial effects at least:

1. each disposable pine wood nematode collecting and separating device is an independent device and is formed by connecting a collecting bottle from bottom to top according to a bottle cap, a funnel, a filter paper sheet and the funnel. The inner side of the bottle cap and the edge of the collection bottle mouth are provided with screw threads which are mutually matched, and the space of the bottle cap is used for collecting sample extracting solution.

2. The disposable pine wood nematode separator that collects utilizes the mobility that soaks sample liquid, and the separator bottleneck is downward, through covering the filter paper piece on the funnel surface, collects the extract in the bottle lid, makes progress the separator bottleneck again, and the extract falls into the funnel bottom, reaches other impurity of getting rid of in the sample fast, and the filtering separation is effectual with collecting the pine wood nematode, the simple operation. Batch separation can be realized according to the number of samples, and resource waste is avoided.

3. The pine wood nematode separator is small in size and easy to carry, samples can be extracted in time during field operation, pine wood nematodes can be separated quickly, and the reliability of results is guaranteed.

4. The pine wood nematode separating device is convenient to collect at one time and is more conveniently used for emergency forest epidemic situations, is convenient for quickly filtering and separating the pine wood nematodes, and is suitable for small and medium-sized forest control stations and the like.

Drawings

FIG. 1: is a schematic diagram of a partial sectional structure of a built-in funnel of the device.

FIG. 2: the partial sectional structure of the bottleneck pressing funnel of the device is shown schematically.

FIG. 3: is a schematic structural diagram of the cross section of the funnel with the circular arc bottom.

FIG. 4: is a schematic sectional structure diagram of a flat-bottom funnel.

FIG. 5: is a top view of the funnel.

The labels in the figure are: 1-collection bottle, 11-collection bottle mouth, 2-bottle cap, 3-funnel A, 31-filtration hole, 32-funnel wall, 33-funnel bottom, 34-funnel mouth, 35-funnel convex edge, 36-bottle cap convex ring, 4-filter paper and 5-funnel B.

Detailed Description

The following provides a further detailed description of embodiments of the present invention with reference to the accompanying drawings. The following examples are merely one of the preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Ordinary changes and substitutions by those skilled in the art within the scope of the present disclosure are intended to be within the scope of the present disclosure.

Example 1

As shown in fig. 1 and fig. 3, a disposable pine wood nematode collecting and separating device is provided with a collecting bottle 1, a collecting bottle opening 11, a bottle cap 2, a funnel A3, filter paper 4 and a funnel B5; a funnel A3 is arranged in a collection bottle opening 11 of the collection bottle 1, and filter paper 4 and a funnel B5 are sequentially arranged in the funnel A3; a bottle cap convex ring 36 is arranged in the bottle cap 2, and the bottle cap 2 is in threaded fit connection with the collecting bottle opening 11 of the collecting bottle 1.

Furthermore, the funnel wall 32 of the funnel A3 at one end of the funnel opening 34 is uniformly provided with a plurality of 2 circles of filtering holes 31, the funnel wall 32 and the funnel bottom 33 are connected into a whole, the funnel opening 34 of the funnel A3 is provided with a funnel convex edge 35, the funnel convex edge 35 is provided with a chamfer vertical convex edge, and the vertical convex edge of the funnel A3 with the vertical convex edge is matched with the inner aperture of the collecting bottle mouth 11.

Further, the outer diameter of the collecting bottle opening 11 is 25 mm.

Further, the diameter of the filtering hole 31 is 1 mm.

Further, the funnel a3 is injection molded.

Further, the depth of the funnel a3 was 7 mm.

Further, the hopper B5 is provided in the same manner as the hopper A3, and the filter paper 4 is provided in a different manner from the hopper A3 and the hopper B5 without the filter holes 31.

Further, the bottle cap bead 36 has a height of 3 mm.

Example 2

Furthermore, the funnel wall 32 of the funnel A3 at one end of the funnel opening 34 is uniformly provided with a plurality of 3 circles of filtering holes 31, the funnel wall 32 and the funnel bottom 33 are connected into a whole, the funnel opening 34 of the funnel A3 is provided with a funnel convex edge 35, the funnel convex edge 35 is provided with a vertical convex edge, and the vertical convex edge of the funnel A3 with the vertical convex edge is matched with the inner aperture of the collecting bottle mouth 11.

Further, the outer diameter of the collecting bottle mouth 11 is 30 mm.

Further, the diameter of the filtering hole 31 is 2 mm.

Further, the funnel a3 is molded using a degradable composite material.

Further, the depth of the funnel a3 is 10 mm.

Further, the height of the cap bead 36 is 4 mm.

Example 3

As shown in fig. 2 and 4, a disposable pine wood nematode collecting and separating device is provided with a collecting bottle 1, a collecting bottle opening 11, a bottle cap 2, a funnel A3, filter paper 4 and a funnel B5; a funnel A3 is arranged in a collection bottle opening 11 of the collection bottle 1, and filter paper 4 and a funnel B5 are sequentially arranged in the funnel A3; a bottle cap convex ring 36 is arranged in the bottle cap 2, and the bottle cap 2 is in threaded fit connection with the collecting bottle opening 11 of the collecting bottle 1.

Furthermore, the funnel wall 32 at one end of the funnel opening 34 of the funnel A3 is uniformly provided with a plurality of 2 circles of filtering holes 31, the funnel wall 32 and the funnel bottom 33 are connected into a whole, the funnel opening 34 of the funnel A3 is provided with a funnel convex edge 35, the funnel convex edge 35 is provided with a flat convex edge, and the outer diameter of the flat convex edge is matched with the outer diameter of the collecting bottle opening 11.

Further, the outer diameter of the collecting bottle mouth 11 is 30 mm.

Further, the diameter of the filtering hole 31 is 1.5 mm.

Further, the funnel a3 is molded using a degradable composite material.

Further, the depth of the funnel a3 is 10 mm.

Further, the height of the cap flange 36 is 5 mm.

The utility model discloses separator's application method:

when in use, the filter paper sheet is wetted by the extracting solution and is absorbed in the funnel A. Putting all samples and extracting solutions into a connecting bottle, clamping a funnel A adsorbing filter paper sheets into a connecting bottle opening, then putting a funnel B into the connecting bottle opening, rotatably connecting a bottle cap, pushing the funnel into the connecting bottle opening by a bottle cap convex ring for 1 mm, inverting the bottle opening of a separating device downwards, releasing and filtering the pine wood nematode extracting solution to store the bottle cap, standing for 6 hours, placing the separating device with the bottle opening upwards, refluxing the sample extracting solution into the funnel, opening the connecting bottle cap, and collecting the sample extracting solution in the funnel for next detection.

Claims

1. The utility model provides a once only collect pine wood nematode separator which characterized in that: the device is provided with a collecting bottle (1), a collecting bottle mouth (11), a bottle cap (2), a funnel A (3), filter paper (4) and a funnel B (5); a funnel A (3) is arranged in a collecting bottle opening (11) of the collecting bottle (1), and filter paper (4) and a funnel B (5) are sequentially arranged in the funnel A (3); a bottle cap convex ring (36) is arranged in the bottle cap (2), and the bottle cap (2) is in threaded fit connection with a collecting bottle opening (11) of the collecting bottle (1).

2. The disposable pine wood nematode separation device of claim 1, wherein: funnel A (3) evenly sets up 1-3 circles at funnel wall (32) of funnel mouth (34) one end and filters hole (31) a plurality of, and funnel wall (32) and funnel bottom (33) are even integrative, and funnel mouth (34) of funnel A (3) set up funnel chimb (35), funnel chimb (35) set up to perpendicular chimb or flat chimb, and the perpendicular chimb of the funnel A (3) of perpendicular chimb matches with the internal diameter of collecting bottleneck (11), and the flat chimb external diameter of the funnel A (3) of flat chimb matches with the external diameter of collecting bottleneck (11).

3. The disposable collecting pine wood nematode separation device of claim 1 or 2, wherein: the outer diameter of the collecting bottle mouth (11) is 25-30 mm.

4. The disposable pine wood nematode separation device of claim 2, wherein: the diameter of the filtering holes (31) is 1-2 mm.

5. The disposable collecting pine wood nematode separation device of claim 1 or 2, wherein: the funnel A (3) is formed by injection molding or is formed by using a degradable composite material to be molded.

6. The disposable collecting pine wood nematode separation device of claim 5, wherein: the depth of the funnel A (3) is 7-10 mm.

7. The disposable pine wood nematode separation device of claim 1, wherein: the setting of funnel B (5) is the same with the setting of funnel A (3), and the setting of filter paper (4) is not the same place with the setting of funnel A (3) and funnel B (5) for not having filtration pore (31).