CN104772272B - A kind of sharp separation acarid and the method for feedstuff - Google Patents
A kind of sharp separation acarid and the method for feedstuff Download PDFInfo
- Publication number
- CN104772272B CN104772272B CN201510095843.9A CN201510095843A CN104772272B CN 104772272 B CN104772272 B CN 104772272B CN 201510095843 A CN201510095843 A CN 201510095843A CN 104772272 B CN104772272 B CN 104772272B
- Authority
- CN
- China
- Prior art keywords
- acarid
- sieve
- feedstuff
- sub
- plastic plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000000926 separation method Methods 0.000 title claims abstract description 6
- 238000012216 screening Methods 0.000 claims abstract description 10
- 239000002023 wood Substances 0.000 claims abstract description 5
- 239000011148 porous material Substances 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000002474 experimental method Methods 0.000 description 12
- 241000611866 Tyrophagus putrescentiae Species 0.000 description 5
- 235000013312 flour Nutrition 0.000 description 5
- 230000000384 rearing effect Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 206010061225 Limb injury Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07B—SEPARATING SOLIDS FROM SOLIDS BY SIEVING, SCREENING, SIFTING OR BY USING GAS CURRENTS; SEPARATING BY OTHER DRY METHODS APPLICABLE TO BULK MATERIAL, e.g. LOOSE ARTICLES FIT TO BE HANDLED LIKE BULK MATERIAL
- B07B1/00—Sieving, screening, sifting, or sorting solid materials using networks, gratings, grids, or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of method that the invention provides sharp separation acarid and feedstuff, step is to take the sample needing to gather acarid from which in right amount, uses the sub-sieve of different pore size to carry out Preliminary screening according to the size of acarid；Uniformly being layered on plastic plate by sample after Preliminary screening, overturn by plastic plate after standing, substantial amounts of acarid and least a portion of feedstuff chip will adsorb on plastic plate；Tip upside down on above thinner sub-sieve by speckling with the plastic plate of sample after preliminary treatment, beat gently with hands or small wood, make all samples fall in sub-sieve, sub-sieve it is inverted after standing, then remaining feedstuff comes off, and remaining in sieve is required acarid entirely, substantially without feedstuff chip.
Description
Technical field
The present invention relates to a kind of method that acarid is isolated and purified with feedstuff, especially the acarid that experiment is cultivated is separated with feedstuff, the method for the acarid needed for quick obtaining lot of experiments.
Background technology
Great many of experiments sample is it is frequently necessary in the analysis or research of acarid biotic experiment.Although and a lot of in the acarid quantity of laboratory rearing, but mix with the feedstuff raising acarid, often enough experiment acarids cannot effectively be collected, obtaining these acarid samples at present typically by the manual method separated by foreign material, owing to its individuality is less, operation more bothers, and use and arrest instrument, when capturing such as brush pen, easily damage demodicid mite class sample and inefficiency, it is unfavorable for next step analysis experiment..So in view of factors above, in the urgent need to a kind of method in experimentation, it is possible to easy and collect the acarid needed for experimentation quickly.
Summary of the invention
It is an object of the invention to provide a kind of the most effective, a large amount of experiment acarids obtaining not damageds clean (few impurity or free from admixture), solution prior art is collected the problem that demodicid mite class wastes time and energy and experiment demodicid mite is produced damage influence final result of study.
For achieving the above object, the present invention is as follows for receiving the method step gathering the required acarid of experiment:
1. take the sample (wherein adulterate feedstuff chip) needing in right amount to gather acarid from which, use the sub-sieve of different pore size to carry out Preliminary screening according to the size of acarid；
2. being uniformly layered on plastic plate by the sample after Preliminary screening, stand 5 minutes, then overturn by plastic plate, substantial amounts of acarid and least a portion of feedstuff chip will adsorb on plastic plate；
3. select a suitable sub-sieve, tip upside down on above sub-sieve by speckling with the plastic plate of sample after preliminary treatment, beat gently with hands or small wood, all samples are made to fall in sub-sieve, after standing 2 minutes, be inverted sub-sieve, then remaining feedstuff comes off, remaining in sieve is required acarid entirely, substantially without feedstuff chip；
4. collecting the acarid finally given to equipped with in the dactylethrae of ethanol, be put in refrigerator, experiment further takes out when needing.
Beneficial effects of the present invention:
The present invention can obtain acarid needed for great many of experiments, prior art inefficiency and poor effect fast and effectively.
The pure chip without feedstuff of acarid that the present invention collects, prior art speckles with substantial amounts of feedstuff chip.
Operating process of the present invention is easy, and prior art operation is loaded down with trivial details and expends a large amount of manpower and materials.
The acarid that the present invention collects is all that living individuals and limbs are complete, and the acarid that prior art collects has certain limb injury more.
The recyclable cultivation of sample after present invention collection, cannot reclaim after sample collection of the prior art.
Detailed description of the invention
The equipment and the reagent that use in experiment include: sub-sieve (140 mesh, 180 mesh and 220 mesh), organic plastic plate (10cm × 25cm × 8mm), 70% ethanol, 1.5ml dactylethrae.
The present invention operates according to following case:
Embodiment 1
Sample, addicted to the collection of flour mite female one-tenth demodicid mite, is poured into inside 140 mesh sub-sieves, is carried out Preliminary screening by the ellipse of laboratory rearing.
The sample stayed after Preliminary screening in sieve is uniformly layered on clean organic plastic plate, after standing 5 minutes, organic plastic plate is overturn, remaining sample is tipped upside down on above 220 mesh sub-sieves after feedstuff chip comes off, beat gently with small wood, comprise the oval sample addicted to flour mite and a small amount of feedstuff chip and fall in sieve, after standing 2 minutes, the 220 mesh sub-sieves filling sample are upside down in and oval raise on device addicted to flour mite, the ellipse of remaining demodicid mite state is recycled addicted to flour mite.Stay in final 220 mesh sub-sieves is oval addicted to flour mite female one-tenth demodicid mite entirely.With the dactylethrae equipped with 70% ethanol, the acarid collection in 220 mesh sub-sieves is used as follow-up experimentation.
Embodiment 2
The collection of the tyrophagus putrescentiae female one-tenth demodicid mite of laboratory rearing, pours into sample inside 180 mesh sub-sieves, carries out Preliminary screening.
The sample stayed after Preliminary screening in sieve is uniformly layered on clean organic plastic plate, after standing 5 minutes, organic plastic plate is overturn, remaining sample is tipped upside down on above 220 mesh sub-sieves after feedstuff chip comes off, beat gently with small wood, the sample comprising tyrophagus putrescentiae and a small amount of feedstuff chip is fallen in sieve, after standing 2 minutes, what the 220 mesh sub-sieves filling sample were upside down in tyrophagus putrescentiae raises on device, is recycled by the tyrophagus putrescentiae of remaining demodicid mite state.Stay in final 220 mesh sub-sieves is tyrophagus putrescentiae female one-tenth demodicid mite entirely.With the dactylethrae equipped with 70% ethanol, the acarid collection in 220 mesh sub-sieves is used as follow-up experimentation.
Claims (3)
1. a sharp separation acarid and the method for feedstuff, it is characterised in that step is as follows:
(1). take the sample needing to gather acarid from which in right amount, use the sub-sieve of different pore size to carry out Preliminary screening according to the size of acarid；
(2). being uniformly layered on plastic plate by the sample after Preliminary screening, stand, then overturn by plastic plate, substantial amounts of acarid and fraction of feedstuff chip will adsorb on plastic plate；
(3). select a suitable sub-sieve, tip upside down on above sub-sieve by speckling with the plastic plate of sample after preliminary treatment, beat gently with hands or small wood, make all samples fall in sub-sieve, stand, be inverted sub-sieve, then remaining feedstuff comes off, and remaining in sieve is required acarid entirely；
(4). the acarid finally given is collected to equipped with in the dactylethrae of ethanol.
Sharp separation acarid the most according to claim 1 and the method for feedstuff, it is characterised in that the sub-sieve that step (1) is used is 140 mesh or 180 mesh sieves.
Sharp separation acarid the most according to claim 1 and the method for feedstuff, it is characterised in that the sub-sieve that step (3) is used is 220 mesh sieves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510095843.9A CN104772272B (en) | 2015-03-04 | 2015-03-04 | A kind of sharp separation acarid and the method for feedstuff |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510095843.9A CN104772272B (en) | 2015-03-04 | 2015-03-04 | A kind of sharp separation acarid and the method for feedstuff |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104772272A CN104772272A (en) | 2015-07-15 |
| CN104772272B true CN104772272B (en) | 2016-11-23 |
Family
ID=53614217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510095843.9A Expired - Fee Related CN104772272B (en) | 2015-03-04 | 2015-03-04 | A kind of sharp separation acarid and the method for feedstuff |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104772272B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3081679B1 (en) * | 2018-06-01 | 2021-11-26 | Ynsect | LAYING MEDIA FOR INSECTS CONTAINING A TEXTURING SUBSTRATE |
| FR3081678B1 (en) * | 2018-06-01 | 2021-10-29 | Ynsect | LAYING MEDIA FOR INSECTS CONTAINING A SOLID SUBSTRATE |
| CN111298515B (en) * | 2019-11-19 | 2021-10-22 | 四川兴华路通再生资源科技有限公司 | Concrete system is retrieved in tank car carwash |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3195490B2 (en) * | 1994-02-15 | 2001-08-06 | 日清製粉株式会社 | Sieve unit |
| CN2894865Y (en) * | 2005-08-05 | 2007-05-02 | 刘玉军 | Internally-enclosed separator |
| CN201270728Y (en) * | 2008-08-21 | 2009-07-15 | 张�杰 | Manure-removing and trash-discharging machine for flour weevil |
| RU2010149554A (en) * | 2010-12-03 | 2012-06-10 | Федеральное государственное образовательное учреждение высшего профессионального образования "Кубанский государственный аграрный у | DEVICE FOR SEPARATION OF BIOHUMUS FROM WORMS |
| CN103299959A (en) * | 2013-05-20 | 2013-09-18 | 中国农业科学院植物保护研究所 | Novel producing method of predatory mites |
-
2015
- 2015-03-04 CN CN201510095843.9A patent/CN104772272B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN104772272A (en) | 2015-07-15 |
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| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161123 Termination date: 20200304 |