CN212560300U - Cell culture plate - Google Patents
Cell culture plate Download PDFInfo
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- CN212560300U CN212560300U CN202020902556.0U CN202020902556U CN212560300U CN 212560300 U CN212560300 U CN 212560300U CN 202020902556 U CN202020902556 U CN 202020902556U CN 212560300 U CN212560300 U CN 212560300U
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- Prior art keywords
- plate
- hole
- cell culture
- opening
- cultivate
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- 238000004113 cell culture Methods 0.000 title claims abstract description 37
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 230000009194 climbing Effects 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 7
- 230000001174 ascending effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000010166 immunofluorescence Methods 0.000 description 3
- 238000003125 immunofluorescent labeling Methods 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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Abstract
The utility model provides a cell culture plate, which belongs to the field of cell culture containers. The cell culture plate comprises: a mounting plate and at least one culture well, each culture well comprising: cultivate the hole curb plate and cultivate the hole bottom plate, it is the cylinder type to cultivate the hole curb plate, first opening and second opening have been seted up respectively to the top and the bottom of cultivateing the hole curb plate, set up on the mounting panel with first opening matched with third opening, it installs on the bottom surface of mounting panel to cultivate the hole curb plate, and first opening and third opening sealing connection, be equipped with the external screw thread on the inner wall of cultivateing the hole curb plate, be provided with on the periphery wall of cultivateing the hole bottom plate with external screw thread matched with internal thread, still be provided with the sealing washer on the periphery wall of cultivateing the hole bottom plate, it installs on the inner wall of cultivateing the. This cell culture plate can be convenient for the experimenter and take away the cell climbing piece, and the difficult condition that takes place breakage or fracture of cell climbing piece in the in-process of taking moreover.
Description
Technical Field
The utility model relates to a cell culture container field, in particular to cell culture plate.
Background
Immunofluorescence techniques, also known as fluorescent antibody techniques, are primarily used to label or detect the corresponding antigen with a fluorescent antibody. In immunofluorescence techniques, cells are cultured using cell culture plates.
Culture wells are provided in conventional cell culture plates, and the number of culture wells is currently 6, 12, 24, 48, 96, 384, 1536, and the like. When carrying out immunofluorescence staining, the use amount problem of dyeing reagent is considered, and the experimenter often can lay the cell slide in advance in the culture hole, makes the cell that awaits measuring grow on the cell slide, takes out the cell slide from culture hole again, then carries out immunofluorescence staining to the cell on the cell slide again.
Cell slide is easy to place in the culture well, but is very difficult to take out. Because glass has good light transmissivity for the cell slide that uses at present is all made by glass, and the thickness of cell slide is less than 1mm, this can guarantee that the cell slide after dyeing can clearly observe fluorescence under the fluorescence microscope, but the glass that thickness is less than 1mm is fragile, therefore often the condition that the cell slide is broken or cracked takes place in the process of taking out the cell slide, once the cell slide breaks or breaks, the experiment is just so interrupted and need carry out immunofluorescence staining again, therefore, need to solve the problem that the cell slide breaks or breaks in the process of taking out.
Disclosure of Invention
In order to solve the problems of the prior art, the embodiment of the utility model provides a cell culture plate. The technical scheme is as follows:
the utility model provides a cell culture plate, cell culture plate includes: a mounting plate and at least one culture well, each culture well comprising: cultivate the hole curb plate and cultivate the hole bottom plate, it is the cylinder type to cultivate the hole curb plate, first opening and second opening have been seted up respectively to the top and the bottom of cultivateing the hole curb plate, seted up on the mounting panel with first opening matched with third opening, it installs to cultivate the hole curb plate on the bottom surface of mounting panel, just first opening with third opening sealing connection, be equipped with the external screw thread on the inner wall of cultivateing the hole curb plate, be provided with on the periphery wall of cultivateing the hole bottom plate with external screw thread matched with internal thread, still be provided with the sealing washer on the periphery wall of cultivateing the hole bottom plate, it passes through to cultivate the hole bottom plate rotatable seal of internal thread install cultivate on the inner wall of hole curb plate.
Specifically, the bottom of the culture well bottom plate is provided with a groove.
Furthermore, the shape of the groove is a straight shape, a cross shape, a meter shape, an inner triangle shape, an inner angle square shape or an inner hexagonal shape.
Specifically, the mounting plate is a light-shielding mounting plate.
Specifically, the cell culture plate further comprises a light-shielding cover plate, and the light-shielding cover plate covers the mounting plate.
Specifically, the cross section of the culture well bottom plate is in a straight line shape.
Specifically, the top surface of the culture hole bottom plate is provided with a boss, and the size of the boss is smaller than that of the cell climbing sheet.
Specifically, the culture wells are 6, 12, 24, 48, 96, 384 or 1536.
The embodiment of the utility model provides a beneficial effect that technical scheme brought is: the embodiment of the utility model provides a pair of cell culture plate, should cultivate hole bottom plate 2b through external force rotation, make and cultivate the hole bottom plate and can realize upwards or decurrent removal along the external screw thread on the inner wall of cultivateing the hole curb plate, when cultivateing the decurrent removal of hole bottom plate, can be with cultivateing the hole bottom plate and cultivateing the separation of hole curb plate, then take away the cell climbing piece again, when cultivateing the ascending removal of hole bottom plate, can be higher than the top surface of mounting panel with the top surface back-spin of cultivateing the hole bottom plate, the experimenter of can being convenient for takes away the cell climbing piece like this, and the cell climbing piece is at the difficult broken or cracked condition that takes place of the in-process of taking, this has improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram of a cell culture plate according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a culture well provided in an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a groove provided in an embodiment of the present invention;
fig. 4 is a schematic structural view of a light-shielding cover plate provided in the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.
Examples
The embodiment of the utility model provides a cell culture plate, as shown in figure 1, this cell culture plate includes: a mounting plate 1 and at least one culture well 2. Each culture well 2 comprises: a culture well side plate 2a and a culture well bottom plate 2 b. Cultivate hole curb plate 2a and be the cylinder type, combine fig. 2, first opening 21a and second opening 22a have been seted up respectively to the top and the bottom of cultivating hole curb plate 2a, set up on mounting panel 1 with first opening 21a matched with third opening 11a, cultivate hole curb plate 2a and install on mounting panel 1's bottom surface, and first opening 21a and third opening 11a sealing connection, be equipped with external screw thread 31 on cultivating hole curb plate 2 a's the inner wall, be provided with on cultivating hole bottom plate 2 b's the periphery wall with external screw thread 31 matched with internal thread 32, still be provided with sealing washer 4 on cultivating hole bottom plate 2 b's the periphery wall, cultivate hole bottom 2b and pass through the rotatable sealing installation of internal screw thread on cultivating hole curb plate 2 a's the inner wall. In this case, the mounting plate 1 and the culture well side plate 2a may have the same size as the corresponding parts of the conventional cell culture plate. This can improve the versatility of the cell culture plate. In this example, the cell culture plate is described by taking 6 culture wells 2 as an example.
As shown in FIG. 3, the bottom of the culture well bottom plate 2b is provided with a groove 5.
Further, the shape of the groove 5 may be rectangular, cross, square, triangular, square with inner angle or hexagonal. When the cell culture plate is used, the cell culture plate can be used together with a screwdriver, the screwdriver (not shown in the figure) matched with the shape of the groove 5 is selected, the head of the screwdriver is placed in the groove 5, the culture hole bottom plate 2b can be driven to rotate by rotating the screwdriver, and the culture hole bottom plate 2b can move up and down through the external threads 31 on the inner wall of the culture hole side plate 2 a. In this embodiment, the shape of the groove 5 is rectangular, and the rectangular groove 5 is convenient to process.
In particular, the mounting plate 1 may be a light-shielding mounting plate. In the immunofluorescence technique, all steps after adding fluorescent antibody need all be gone on under the condition of keeping away from the light, especially when carrying out line laser copolymerization focal operation, the environment of keeping away from the light can be provided to the light-proof mounting panel, can prevent that culture hole 2 from receiving the irradiation of scattered light and avoid taking place fluorescence quenching to guarantee that the experiment goes on smoothly, when realizing, the light-proof mounting panel can adopt and scribble dark coating at transparent mounting panel 1, can also cover the shading coating on mounting panel 1. The culture hole 2 is still made of transparent material, so that the influence on the addition of the culture medium and other operations after the light-shielding material is added can be avoided.
As shown in FIG. 4, the cell culture plate may further include a light-shielding cover 6, and the light-shielding cover 6 covers the mounting plate 1. The light-shielding cover plate 6 can further prevent scattered light from being irradiated into the culture hole 2 in the cell culture stage, so that the success rate of the experiment is improved.
Referring again to FIG. 1, the cross-section of the well bottom plate 2b may be rectangular. The top surface of rectangular cultivation hole bottom plate 2b can be convenient for lay cell climbing piece a, and the bottom surface of rectangular cultivation hole bottom plate 2b can be convenient for set up recess 5.
Specifically, the culture wells 2 may be 6, 12, 24, 48, 96, 384, or 1536.
As shown in FIG. 2, a projection 7 may be provided on the top surface of the culture well bottom plate 2b, and the size of the projection 7 is smaller than that of the cell-climbing sheet a. When cell climbing piece a needs to be taken out, experimenters can take the cell climbing piece a away along the edge of boss 7, so that the experimenters can take out the cell climbing piece a.
The following briefly introduces the working principle of the cell culture plate provided by the embodiment of the present invention, which is as follows:
put cell climbing piece an and carry out cell culture on the top surface of cultivateing hole bottom plate 2b, treat the cell culture back that finishes, abandon the culture medium in the cultivation hole 2, should cultivate hole bottom plate 2b through external force rotation, make cultivation hole bottom plate 2b can realize upwards or decurrent removal along external screw thread 31 on the inner wall of cultivation hole curb plate 2a, when cultivateing the decurrent removal of hole bottom plate 2b, can separate cultivation hole bottom plate 2b and cultivation hole curb plate 2a, then take cell climbing piece an away again, when cultivateing the ascending removal of hole bottom plate 2b, can be with the top surface of cultivateing hole bottom plate 2b screw out and be higher than the top surface of mounting panel 1, the experimenter of can being convenient for like this takes cell climbing piece an away.
The embodiment of the utility model provides a pair of cell culture plate, should cultivate hole bottom plate 2b through external force rotation, make and cultivate the hole bottom plate and can realize upwards or decurrent removal along the external screw thread on the inner wall of cultivateing the hole curb plate, when cultivateing the decurrent removal of hole bottom plate, can be with cultivateing the hole bottom plate and cultivateing the separation of hole curb plate, then take away the cell climbing piece again, when cultivateing the ascending removal of hole bottom plate, can be higher than the top surface of mounting panel with the top surface back-spin of cultivateing the hole bottom plate, the experimenter of can being convenient for takes away the cell climbing piece like this, and the cell climbing piece is at the difficult broken or cracked condition that takes place of the in-process of taking, this has improved.
The above description is only an optional embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A cell culture plate, comprising: a mounting plate and at least one culture well, each culture well comprising: cultivate the hole curb plate and cultivate the hole bottom plate, it is the cylinder type to cultivate the hole curb plate, first opening and second opening have been seted up respectively to the top and the bottom of cultivateing the hole curb plate, seted up on the mounting panel with first opening matched with third opening, it installs to cultivate the hole curb plate on the bottom surface of mounting panel, just first opening with third opening sealing connection, be equipped with the external screw thread on the inner wall of cultivateing the hole curb plate, be provided with on the periphery wall of cultivateing the hole bottom plate with external screw thread matched with internal thread, still be provided with the sealing washer on the periphery wall of cultivateing the hole bottom plate, it passes through to cultivate the hole bottom plate rotatable seal of internal thread install cultivate on the inner wall of hole curb plate.
2. The cell culture plate of claim 1, wherein the bottom of the culture well bottom plate is provided with a groove.
3. The cell culture plate of claim 2, wherein the shape of the groove is a straight shape, a cross shape, a meter shape, an inner triangular shape, an inner square shape, or an inner hexagonal shape.
4. The cell culture plate of claim 1, wherein the mounting plate is a light-shielding mounting plate.
5. The cell culture plate of claim 1, further comprising a light-shielding cover plate overlying the mounting plate.
6. The cell culture plate of claim 1, wherein the culture well bottom plate is in-line in cross-section.
7. The cell culture plate according to claim 1, wherein a projection is provided on the top surface of the culture well bottom plate, and the size of the projection is smaller than that of the cell slide.
8. The cell culture plate of claim 1, wherein the culture wells are 6, 12, 24, 48, 96, 384, or 1536.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020902556.0U CN212560300U (en) | 2020-05-26 | 2020-05-26 | Cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020902556.0U CN212560300U (en) | 2020-05-26 | 2020-05-26 | Cell culture plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212560300U true CN212560300U (en) | 2021-02-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020902556.0U Expired - Fee Related CN212560300U (en) | 2020-05-26 | 2020-05-26 | Cell culture plate |
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| CN (1) | CN212560300U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113640089A (en) * | 2021-09-17 | 2021-11-12 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
-
2020
- 2020-05-26 CN CN202020902556.0U patent/CN212560300U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113640089A (en) * | 2021-09-17 | 2021-11-12 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
| CN113640089B (en) * | 2021-09-17 | 2022-02-08 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210219 |