CN113640089B - Cell immunohistochemical staining device - Google Patents
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- CN113640089B CN113640089B CN202111094549.8A CN202111094549A CN113640089B CN 113640089 B CN113640089 B CN 113640089B CN 202111094549 A CN202111094549 A CN 202111094549A CN 113640089 B CN113640089 B CN 113640089B
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- 238000011532 immunohistochemical staining Methods 0.000 title claims abstract description 22
- 238000004113 cell culture Methods 0.000 claims abstract description 61
- 239000011148 porous material Substances 0.000 claims abstract description 24
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 18
- 239000011159 matrix material Substances 0.000 claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims description 87
- 230000009194 climbing Effects 0.000 claims description 21
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 3
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 3
- 241001330002 Bambuseae Species 0.000 claims description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 3
- 239000011425 bamboo Substances 0.000 claims description 3
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 2
- 230000001413 cellular effect Effects 0.000 claims 9
- 238000010186 staining Methods 0.000 abstract description 15
- 238000002474 experimental method Methods 0.000 description 27
- 238000004043 dyeing Methods 0.000 description 11
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000006143 cell culture medium Substances 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 238000003125 immunofluorescent labeling Methods 0.000 description 2
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- 239000011248 coating agent Substances 0.000 description 1
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- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
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Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
Abstract
A cell immunohistochemical staining device comprises a transparent cell culture pore plate, wherein a plurality of culture holes distributed in a matrix are arranged on the cell culture pore plate, a transparent culture cylinder is respectively placed in each culture hole, the culture cylinder comprises a hydrophobic left half cylinder body and a hydrophobic right half cylinder body, the upper ends of the left half cylinder body and the right half cylinder body are connected through hinges, the lower ends of the left half cylinder body and the right half cylinder body are respectively provided with supporting legs for fixing cell crawlers, the inner sides of the supporting legs are provided with clamping grooves, the left half cylinder body and the right half cylinder body are oppositely folded, a circular cell crawler is arranged between the supporting legs of the left half cylinder body and the right half cylinder body, the cell crawlers are fixed through the clamping grooves of the supporting legs in a clamping mode to form a culture cylinder which can take the cell crawlers down by separating the lower ends of the left half cylinder body and the right half cylinder body, the upper end of the cell culture pore plate is covered with a transparent upper cover, and the cell immunohistochemical staining from cell culture to the cell immunochemistry staining can be completed in the cell culture pore plate, the experimental efficiency and the success rate can be improved.
Description
Technical Field
The invention relates to the technical field of cell immune staining, in particular to a cell immunohistochemical staining device.
Background
Cell culture and cell immunohistochemical staining are routine experiments in the biological field, but the steps involved in the cell immunohistochemical staining are complicated, and at present, the routine operation method is to directly put a cell slide matched with a culture well plate into a culture well, then to perform cell culture in the culture well, and to fix cells for staining after the cell culture is completed. However, the process involves many steps, and since some expensive antibodies are often used in the cytoimmunohistochemical staining experiment or the cytoimmunofluorescent staining experiment, while the conventional cell culture well plate is not subjected to hydrophobic treatment, if the staining operation is directly performed in the culture well, leakage is likely to occur, which results in cell depletion, and a large amount of antibody is likely to be lost, which results in dry plate, a large amount of antibodies must be used during the experiment, thereby causing reagent waste and increasing the experiment cost, therefore, cell samples need to be transferred for many times during the operation, the samples are easy to fall off when being transferred, since the cell slide is usually a fragile transparent sheet, frequent clamping of the cell slide also easily causes rupture of the cell slide, thereby leading to the experiment failure to and transfer out that the sample is difficult to distinguish which side of cell culture at the cell slide, consequently the experiment success rate is lower.
Disclosure of Invention
The invention aims to provide a cell immunohistochemical staining device aiming at the defects of the prior art, which can complete the cell culture and the cytoimmunochemical staining in a culture cylinder without frequently transferring cell crawl sheets, can avoid the cell crawl sheets from overturning, save antibody reagents, simplify experiment steps and greatly improve the experiment efficiency and success rate.
The technical scheme of the invention is as follows: the utility model provides a cell immunohistochemical dyeing apparatus, includes transparent cell culture orifice plate, set up a plurality of cultivation holes that are the matrix distribution on the cell culture orifice plate, place a transparent culture cylinder in each cultivation hole respectively, the culture cylinder includes hydrophobic left half barrel, right half barrel, the upper end of left half barrel, right half barrel links to each other through articulated, the lower extreme of left half barrel, right half barrel sets up the supporting legs that is used for fixed cell climbing piece respectively, and the inboard of each supporting leg is equipped with the draw-in groove, left half barrel and right half barrel fold in opposite directions, set up a cell climbing piece between the supporting legs of left half barrel, right half barrel, the cell climbing piece is fixed by the draw-in groove joint of supporting legs, forms the culture cylinder that can take off the cell climbing piece separately left half barrel, right half barrel lower extreme, cell culture orifice plate upper end cover goes up transparent upper cover.
Further, upper end one side of half barrel on the left side sets up first articulated seat, and the upper end opposite side of half barrel on the left side sets up first articulated round pin, upper end one side of half barrel on the right side corresponds first articulated seat and sets up the articulated cooperation of second articulated round pin formation, and the upper end opposite side of half barrel on the right side corresponds first articulated pin and sets up the articulated cooperation of second articulated seat formation.
Further, equally divide on the articulated seat of first articulated seat, the second and do not be equipped with the groove of stepping down of axial extension, the symmetry sets up the hinge hole that communicates with the groove of stepping down on the cell wall of groove both sides of stepping down, the both sides of first articulated round pin, the articulated round pin of second are equallyd divide and do not set up outside convex articulated arch, make two articulated seats and two articulated round pins respectively through hinge hole, articulated protruding formation hinge cooperation.
Furthermore, the first hinge pin is inserted into the second hinge seat, and one end of the second hinge pin inserted into the first hinge seat is respectively configured to be arc-shaped.
Furthermore, the hinge hole is a strip-shaped hole, and the hinge protrusion is a spherical protrusion.
Furthermore, the upper end of the cell culture pore plate is provided with a fixing frame, the fixing frame is provided with a plurality of abdicating openings which are arranged in a matrix form by criss-cross fixing rods, and the fixing rods around the abdicating openings are respectively provided with a protruding part which extends to the upper ends of the left half-cylinder body and the right half-cylinder body and is used for pressing the culture cylinder.
Further, the dead lever end of mount is equipped with a plurality of resilient clip, resilient clip is "U" shape bending form, be equipped with fixed slot and resilient clip joint on the cell culture orifice plate.
Furthermore, the bottom of the culture hole of the cell culture hole plate is provided with a positioning groove, and the positioning groove corresponds to the supporting legs of the left half cylinder and the right half cylinder respectively, so that the cell climbing sheet is contacted with the bottom of the culture hole.
Furthermore, four corners of the cell culture pore plate are respectively provided with a boss, and the cell culture pore plate and the upper cover are correspondingly provided with oblique angles for distinguishing directions.
Further, a hydrophobic membrane is coated on the cell culture pore plate for hydrophobic treatment.
Adopt above-mentioned technical scheme: the device places a culture cylinder in a culture hole of a cell culture pore plate, the culture cylinder is folded by a left half cylinder body and a right half cylinder body in opposite directions, wherein the left half cylinder body and the right half cylinder body both have hydrophobic property, and a cell climbing sheet is clamped between the left half cylinder body and the right half cylinder body. When the cell culture plate is used, required cells can be directly cultured on the cell slide in the culture cylinder, and the cell culture plate, the culture cylinder and the upper cover are all transparent, so that the phase contrast microscope is convenient to observe the culture condition of the cells in the cell culture process. After the cell culture is finished, as the device adopts the hydrophobic left half cylinder body and the hydrophobic right half cylinder body, no leakage occurs, thus the cell slide does not need to be taken out, the culture cylinder can be continuously placed in the culture hole, and the cytoimmunochemistry staining experiment can be directly carried out on the cell climbing sheet in the culture cylinder, thus not only saving the use of antibodies in the staining experiment, but also effectively preventing the accidental situations such as dry sheet and the like, the situation that the cell is on which side cannot be distinguished can not occur, after the experiment is finished, the culture cylinder is taken out from the culture hole by hand, so that the left half cylinder body, the right half cylinder body and the cell climbing sheet are integrally taken out from the culture hole, the culture cylinder can be vertically taken out from the culture hole when taken out, the cell climbing sheet cannot be inclined or overturned, the cell is prevented from being lost, then the lower ends of the left half cylinder body and the right half cylinder body are separated, and the cell slide can be taken down for sealing and storing and the like. This device is from cell culture to completion in the culture section of thick bamboo that cell immunochemistry dyeing can all be in the cell culture orifice plate, and the cell slide is removed in the frequent centre gripping of experimentation need not, can effectively prevent the emergence of unexpected circumstances such as cell slide upset, antibody overflow to greatly practice thrift cell culture medium and the required antibody of dyeing, guarantee cell quantity and quality, and improve experimental efficiency and experiment success rate, provide the assurance for obtaining high-quality immunocyte dyeing picture. In addition, the device can be used for multicolor cell immunofluorescence staining, high-quality cell staining pictures can be obtained, and the experimental steps can be simplified due to the use of the device, so that a novice can quickly master the experimental method of the cell immunohistochemical staining.
The invention is further described with reference to the drawings and the specific embodiments in the following description.
Drawings
FIG. 1 is an exploded view of the present invention;
FIG. 2 is a schematic structural view of the left half cylinder of the present invention;
FIG. 3 is a top view of the left and right cartridge halves of the present invention;
FIG. 4 is a schematic view of the assembly of the culture cylinder of the present invention;
FIG. 5 is a schematic diagram of the structure of a cell culture well plate according to the present invention;
FIG. 6 is a schematic structural view of the fixing frame of the present invention;
fig. 7 is a schematic structural view of the upper cover of the present invention.
Detailed Description
Referring to fig. 1 to 7, a cell immunohistochemical staining apparatus includes a transparent cell culture well plate 1, a plurality of culture wells 2 distributed in a matrix are disposed on the cell culture well plate 1, and a transparent culture cylinder 3 is disposed in each culture well 2. The culture cylinder 3 comprises a hydrophobic left half cylinder body 4 and a hydrophobic right half cylinder body 5, the hydrophobic treatment can be carried out on the left half cylinder body 4 and the right half cylinder body 5 by adopting a mode of coating a hydrophobic film, liquid leakage of a liquid reagent in an experiment can be prevented, and therefore, a cell slide 6 does not need to be taken out when a cytochemical staining experiment is carried out, the culture cylinder 3 can be continuously placed in the culture hole 2 for experimental operation, so that required antibodies in the staining experiment are greatly saved, and the experimental cost is saved. In addition, a hydrophobic membrane can be coated on the cell culture pore plate 1 for hydrophobic treatment, so that the risk of liquid leakage of a liquid reagent is further reduced, the cell culture and the cytoimmunochemical staining can be completed in the culture cylinder 3 in the cell culture pore plate 1, and the cell slide does not need to be clamped and moved back and forth. The upper ends of the left half cylinder body 4 and the right half cylinder body 5 are connected through hinge joint, so that the upper ends of the left half cylinder body 4 and the right half cylinder body 5 can rotate relatively, the lower ends of the left half cylinder body 4 and the right half cylinder body 5 are respectively provided with supporting legs 7 for fixing cell climbing sheets 6, the inner sides of the supporting legs 7 are provided with clamping grooves 8, the left half cylinder body 4 and the right half cylinder body 5 are folded oppositely, a round cell climbing sheet 6 is arranged between the supporting legs 7 of the left half cylinder body 4 and the right half cylinder body 5, the cell climbing sheet 6 is clamped and fixed by the clamping grooves 8 of the supporting legs 7, the culture cylinder 3 of the cell climbing sheet 6 can be taken down by separating the lower ends of the left half cylinder body 4 and the right half cylinder body 5, therefore, when the cell climbing sheet 6 needs to be transferred, the culture cylinder 3 only needs to be held by hand to be vertically lifted out from the culture hole 2, then the lower ends of the left half cylinder body 4 and the right half cylinder body 5 are separated, and the cell climbing sheet 6 can be taken out from the clamping grooves 8 of the supporting legs 7, in the whole transfer process, can guarantee that cell slide 6 places the one end of cell sample towards the top all the time, prevent that cell slide 6 from taking place to overturn or slope in the transfer process, guarantee the cell quantity on cell slide 6. Cell culture orifice plate 1 cultivates 2 hole bottoms in hole and is equipped with constant head tank 23, constant head tank 23 corresponds with the supporting legs 7 of left half barrel 4, right half barrel 5 respectively, makes cell climbing piece 6 and the contact of the hole bottom of cultivateing hole 2 to make there is not the clearance between cell climbing piece 6 and the hole bottom of cultivateing hole 2, prevent that liquid reagent from remaining in the clearance in cell climbing piece 6 and cultivation hole 2. The upper end of the cell culture pore plate 1 is covered with the transparent upper cover 9, and the cell culture pore plate 1, the culture cylinder 3 and the upper cover 9 are all transparent, so that the device can be directly moved to a phase contrast microscope for observation, and the cell condition can be conveniently observed by using the phase contrast microscope in the cell culture process and a dyeing experiment. The four corners of cell culture orifice plate 1 are equipped with boss 24 respectively, make upper cover 9 lid when cell culture orifice plate 1 upper end, cell culture orifice plate 1 can support the inner wall of upper cover 9 through boss 24, strengthens upper cover 9 and cell culture orifice plate 1's reliability of being connected, cell culture orifice plate 1 and upper cover 9 correspond to be equipped with the oblique angle that is used for distinguishing the direction, improve the convenience of using. Moreover, the cell culture pore plate 1, the culture cylinder 3 and the upper cover 9 can be made of high-temperature-resistant plastics, so that the device can be suitable for the temperature of 4-100 ℃, and can adapt to the temperature change in the process of multicolor immunofluorescence dyeing.
In this embodiment, upper end one side of the half barrel body 4 in a left side sets up first articulated seat 10, and the upper end opposite side of the half barrel body 4 in a left side sets up first hinge pin 11, upper end one side of the half barrel body 5 in a right side corresponds first articulated seat 10 and sets up second hinge pin 12 and form articulated cooperation, and the upper end opposite side of the half barrel body 5 in a right side corresponds first hinge pin 11 and sets up second articulated seat 13 and form articulated cooperation, makes the half barrel body 4 in a left side and the half barrel body 5 in a right side articulate through articulated seat, the hinge pin that sets up relatively and link to each other. Equally divide on first articulated seat 10, the articulated seat 13 of second and do not be equipped with axially extended groove 14 of stepping down, the symmetry sets up the hinge hole 15 with groove 14 intercommunication of stepping down on the cell wall of groove 14 both sides of stepping down, first hinge pin 11, the both sides of second hinge pin 12 are equallyd divide and do not set up outside convex articulated arch 16, make two articulated seats and two hinge pins respectively through hinge hole 15, articulated arch 16 form articulated cooperation, during the assembly, insert corresponding articulated seat along groove 14 of stepping down with the hinge pin of left half barrel 4, right half barrel 5 respectively, until articulated arch 16 on the hinge pin insert in hinge hole 15, can make the upper end of left half barrel 4 and right half barrel 5 form articulated cooperation, hinge hole 15 is the bar hole, reserves the motion space for articulated arch 16, make articulated arch 16 can follow the extending direction motion of hinge hole 15, articulated arch 16 is the sphere arch, the hinge protrusion 16 may be inserted into the hinge hole 15 to provide a guide function, thereby facilitating assembly. The ends of the first hinge pin 11 inserted into the first hinge seat 10 and the second hinge pin 12 inserted into the second hinge seat 13 are respectively configured as circular arcs, which facilitates the rotation of the two hinge pins in the corresponding hinge seats. The left half cylinder body 4 and the right half cylinder body 5 of the embodiment are hinged by adopting the mode, the assembly is reliable, and the purpose of taking out the cell climbing sheet 6 from the lower ends of the left half cylinder body 4 and the right half cylinder body 5 by rotating the upper ends of the left half cylinder body 4 and the right half cylinder body 5 can be achieved.
This embodiment, further can also set up a mount 17 in the upper end of cell culture orifice plate 1, mount 17 forms a plurality of openings of stepping down 19 that are the matrix arrangement by the dead lever 18 of vertically and horizontally crossing, should step down opening 19 and play the effect of stepping down for culture cylinder 3, the experimenter of being convenient for operates, it is equipped with the bulge 20 that extends to left half barrel 4, the upper end of right half barrel 5 respectively on 19 dead lever 18 all around to let a position for push down culture cylinder 3, play the spacing conduct of axial to culture cylinder 3, make culture cylinder 3 fix in culture hole 2. The 18 ends of dead lever of mount 17 are equipped with a plurality of resilient clip 21, resilient clip 21 is "U" shape crooked form, be equipped with fixed slot 22 and resilient clip 21 joint on the cell culture orifice plate 1, during the installation, the open end of mediating resilient clip 21 makes crooked end insert in fixed slot 22, then loosen the open end and can go into resilient clip 21 card in fixed slot 22, utilize resilient clip 21's elastic deformation to fix the upper end at cell culture orifice plate 1 with mount 17, the dismouting is simple convenient.
When the device is used, required cells can be directly cultured on the cell slide 6 in the culture cylinder 3, after cell culture is completed, because the left half cylinder body 4, the right half cylinder body 5 and the cell culture pore plate 1 of the device have hydrophobicity, the condition of liquid leakage can not occur, the cell slide 6 does not need to be taken out, the culture cylinder 3 can be continuously placed in the culture pore 2, and the cell immunochemistry or immunofluorescence staining experiment can be directly carried out on the cell slide 6, so that the required antibodies in the culture medium and the staining experiment can be saved, the unexpected conditions such as dry cell caused by antibody loss can be effectively prevented, one end of the cell slide 6 cultured with the cells in the experiment process always faces upwards, the condition that the cells are in which plane can not be distinguished can not occur, after the experiment is completed, the culture cylinder 3 is held by hand to be vertically taken out from the culture pore 2, so that the left half cylinder body 4, The right half cylinder body 5 and the cell slide 6 are integrally taken out from the culture hole 2, the cell slide 6 cannot be inclined or overturned, the cell is prevented from being lost, and then the cell slide 6 can be taken down to be sealed and stored by separating the lower ends of the left half cylinder body 4 and the right half cylinder body 5. Because the cell culture hole plate 1, the culture cylinder 3 and the upper cover 9 are all transparent, the whole device can be placed under a phase contrast microscope to observe the cell condition in the cell culture process and the dyeing experiment.
This device is from cell culture to completion in the culture section of thick bamboo 3 of cell immunochemistry dyeing homoenergetic in cell culture orifice plate 1, cell slide 6 is removed to the frequent centre gripping of experimentation need not, can prevent effectively that cell slide 6 from overturning, the emergence of unexpected condition such as antibody overflow, thereby greatly practice thrift the volume of cell culture medium and the required antibody of dyeing, practice thrift the experimentation cost, guarantee cell quantity and quality simultaneously, and improve experimental efficiency and experiment success rate, provide the assurance for obtaining high-quality immune cell dyeing picture. In addition, use this device can also carry out polychrome cell immunofluorescence dyeing, can obtain high-quality cell staining picture equally, because use this device can simplify the experimental procedure, reduce the degree of difficulty of cell immunohistochemical staining experiment, consequently, be favorable to the novice to master the experimental method of cell immunohistochemical staining fast.
Claims (10)
1. A cell immunohistochemical staining device comprising a transparent cell culture well plate (1), characterized in that: the cell culture pore plate (1) is provided with a plurality of culture holes (2) distributed in a matrix, a transparent culture cylinder (3) is respectively placed in each culture hole (2), the culture cylinder (3) comprises a left half cylinder body (4) and a right half cylinder body (5) which are hydrophobic, the upper ends of the left half cylinder body (4) and the right half cylinder body (5) are connected through hinges, the lower ends of the left half cylinder body (4) and the right half cylinder body (5) are respectively provided with supporting legs (7) used for fixing cell crawlers (6), the inner side of each supporting leg (7) is provided with a clamping groove (8), the left half cylinder body (4) and the right half cylinder body (5) are oppositely folded, a round cell crawlers (6) are arranged between the supporting legs (7) of the left half cylinder body (4) and the right half cylinder body (5), and the cell crawlers (6) are clamped and fixed by the clamping grooves (8) of the supporting legs (7), the culture cylinder (3) which can be used for separating the lower ends of the left half cylinder body (4) and the right half cylinder body (5) and taking down the cell climbing sheet (6) is formed, and the upper end of the cell culture pore plate (1) is covered with a transparent upper cover (9).
2. The cellular immunohistochemical staining apparatus of claim 1, wherein: the upper end one side of half barrel (4) in a left side sets up first articulated seat (10), and the upper end opposite side of half barrel (4) in a left side sets up first articulated round pin (11), the upper end one side of half barrel on the right side (5) corresponds first articulated seat (10) and sets up second articulated round pin (12) and form articulated cooperation, and the upper end opposite side of half barrel on the right side corresponds first articulated round pin (11) and sets up articulated seat (13) of second and form articulated cooperation.
3. The cellular immunohistochemical staining apparatus of claim 2, wherein: equally divide on first articulated seat (10), the articulated seat of second (13) and do not be equipped with axial extension's groove of stepping down (14), the symmetry sets up hinge hole (15) that feeds through with groove of stepping down (14) on the cell wall of groove of stepping down (14) both sides, the both sides of first articulated round pin (11), the articulated round pin of second (12) are equallyd divide and do not set up outside convex articulated protruding (16), make two articulated seats and two articulated round pins respectively through hinge hole (15), articulated protruding (16) formation hinge cooperation.
4. The cellular immunohistochemical staining apparatus of claim 2, wherein: the first hinge pin (11) is inserted into the second hinge seat (13), and one end of the second hinge pin (12) inserted into the first hinge seat (10) is respectively provided with an arc shape.
5. The cellular immunohistochemical staining apparatus of claim 3, wherein: the hinge holes (15) are strip-shaped holes, and the hinge protrusions (16) are spherical protrusions.
6. The cellular immunohistochemical staining apparatus of claim 1, wherein: the upper end of cell culture orifice plate (1) sets up a mount (17), mount (17) form a plurality of matrix arrangement's of letting position mouth (19) by dead lever (18) that move about freely and quickly alternately, let and be equipped with respectively on position mouth (19) dead lever (18) all around and extend to left half barrel (4), bulge (20) of right half barrel (5) upper end for push down culture section of thick bamboo (3).
7. The cellular immunohistochemical staining apparatus of claim 6, wherein: the end of the fixing rod (18) of the fixing frame (17) is provided with a plurality of elastic clips (21), each elastic clip (21) is in a U-shaped bending shape, and the cell culture pore plate (1) is provided with a fixing groove (22) and an elastic clip (21) in a clamping connection mode.
8. The cellular immunohistochemical staining apparatus of claim 1, wherein: the bottom of the culture hole (2) of the cell culture pore plate (1) is provided with a positioning groove (23), the positioning groove (23) respectively corresponds to the supporting legs (7) of the left half cylinder body (4) and the right half cylinder body (5), so that the cell climbing sheet (6) is contacted with the bottom of the culture hole (2).
9. The cellular immunohistochemical staining apparatus of claim 1, wherein: bosses (24) are respectively arranged at four corners of the cell culture pore plate (1), and oblique angles for distinguishing directions are correspondingly arranged on the cell culture pore plate (1) and the upper cover (9).
10. The cellular immunohistochemical staining apparatus of claim 1, wherein: and a hydrophobic membrane is coated on the cell culture pore plate (1) for hydrophobic treatment.
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| CN214032536U (en) * | 2020-07-06 | 2021-08-24 | 广州华越肾科再生医学科技有限公司 | Cell culture plate without damaging matrigel and cells |
| CN213086005U (en) * | 2020-08-19 | 2021-04-30 | 苏州大学 | Cell climbing sheet culture matching device |
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