CN109943466A - A kind of cell climbing sheet grabber - Google Patents
A kind of cell climbing sheet grabber Download PDFInfo
- Publication number
- CN109943466A CN109943466A CN201910223995.0A CN201910223995A CN109943466A CN 109943466 A CN109943466 A CN 109943466A CN 201910223995 A CN201910223995 A CN 201910223995A CN 109943466 A CN109943466 A CN 109943466A
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- China
- Prior art keywords
- hollow cylinder
- push rod
- limit hole
- climbing sheet
- cell climbing
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- 230000009194 climbing Effects 0.000 title claims abstract description 39
- 239000000463 material Substances 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000001464 adherent effect Effects 0.000 description 2
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of cell climbing sheet grabbers, including hollow cylinder, push rod and cell climbing sheet, hollow cylinder is internally connected with push rod, and push rod and hollow cylinder dismantling connection, the bottom end of hollow cylinder is fixed with sucker, surface a side of hollow cylinder offers high-order limit hole and low level limit hole, top a side of hollow cylinder is connected with by pressure arm, the interior bottom portion of hollow cylinder is fixed with reset spring, the bottom end of push rod is fixed with rubber plug compatible with hollow cylinder, the bottom side side of push rod is connected with elastic spacing device, and elastic spacing device is connect by tilting bar with push rod, the diameter of elastic spacing device is adapted with the diameter of high-order limit hole and low level limit hole.The utility model has the advantages that cell climbing sheet grabber of the invention can easily, quickly grab cell climbing sheet, because of the probability that misoperation causes creep plate broken when reducing crawl creep plate, provide convenience to carry out related experiment.
Description
Technical field
The present invention relates to the transfers of cell climbing sheet and isolated equipment technology field, it particularly relates to which a kind of cell is climbed
Piece grabber.
Background technique
Cell climbing sheet technology, which refers to, allows slide to be immersed in cell culture medium, and cell is grown on slide, is mainly used for tissue
It learns, immunohistochemistry, in situ hybridization etc..The slide used in this technology is also referred to as cell climbing sheet, and special place is passed through on general surface
Reason, is more suitable for the adherency of attached cell.Creep plate is respectively suitable for a thickness of 0.17mm, diameter for 8mm/14mm/20mm/25mm.
48 porocyte culture plates/24 porocyte culture plates/12 porocyte culture plates/6 porocyte culture plates.Traditional operating procedure is:
1. cell climbing sheet sterilizes.
2. creep plate, is then placed on drop, compression (passes through the work of surface tension by 1 drop culture medium of drop in the every hole of culture plate
It is adsorbed on culture plate with by creep plate).
3. regular growth digests, it is centrifuged, is resuspended, the cell suspension of piping and druming is added separately on each creep plate, or directly
Routinely inoculating cell is to culture hole, allows its adherent growth.
4. sucking cell culture fluid, washed three times with PBS, 4% paraformaldehyde or other fixatives are fixed.
5. after finishing cell climbing sheet, carefully being taken out creep plate with tweezers, subsequent detection is carried out.
Wherein adherent creep plate is difficult to be taken out with conventional pincet, and creep plate is easily caught broken when being removed, this is also this
The maximum defect of method.The method or instrument for lacking crawl creep plate that can be simple and safe at present are directed to the problems in the relevant technologies,
Currently no effective solution has been proposed.
Summary of the invention
Technical assignment of the invention is against the above deficiency, a kind of cell climbing sheet grabber to be provided, to solve the above problems.
The technical scheme of the present invention is realized as follows:
A kind of cell climbing sheet grabber, including hollow cylinder, push rod and cell climbing sheet, the inside connection of the hollow cylinder
There are the push rod, and the push rod and the hollow cylinder dismantling connection, the diameter of the bottom end of the hollow cylinder is less than described
The diameter on the top of hollow cylinder, and the bottom end of the hollow cylinder is pyramidal structure, the bottom end of the hollow cylinder is fixed with
Sucker, surface a side of the hollow cylinder offer high-order limit hole and low level limit hole, the top of the hollow cylinder
A side is connected with by pressure arm, and the interior bottom portion of the hollow cylinder is fixed with reset spring, and the bottom end of the push rod is fixed with
Rubber plug compatible with the hollow cylinder, the bottom side side of the push rod are connected with elastic spacing device, and the elasticity limit
Position device is connect by tilting bar with the push rod, and the diameter of the elastic spacing device and the high-order limit hole and the low level limit
The diameter in position hole is adapted.
Further, the high-order limit hole, low level limit hole and the pressure arm of pressing are located at the same of the hollow cylinder
Side, and be located in same horizontal line.
Further, the rubber plug and the hollow cylinder are interference fitted, and the bottom diameter of the rubber plug is less than described
The top end diameter of rubber plug.
Further, the top of the push rod is fixed with pressing plate, and the pressing plate is round pressing plate.
Further, the vertical height by pressure arm and the low level limit hole are in same level, the pressing
The bottom end of arm and the press surface that protrusion is fixed with close to a side of the hollow cylinder.
Further, the sucker is rubber material.
Beneficial effects of the present invention:
1, cell climbing sheet grabber of the invention can easily, quickly grab cell climbing sheet, reduce crawl creep plate
When cause creep plate broken because of misoperation probability, provide convenience to carry out related experiment.
2, the configuration of the present invention is simple, it is easy to operate, it is more convenient for people to use and cell climbing sheet is grabbed.
Detailed description of the invention
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is a kind of cell climbing sheet grabber schematic diagram according to an embodiment of the present invention；
Fig. 2 is the structural schematic diagram of cell climbing sheet according to an embodiment of the present invention；
Fig. 3 is the structural schematic diagram of hollow cylinder according to an embodiment of the present invention；
Fig. 4 is the structural schematic diagram of push rod according to an embodiment of the present invention.
In figure:
1, hollow cylinder；2, push rod；3, cell climbing sheet；4, sucker；5, high-order limit hole；6, low level limit hole；7, it presses
Arm；8, reset spring；9, rubber plug；10, elastic spacing device；11, tilting bar；12, pressing plate.
Specific embodiment
In the description of the present invention, it is to be understood that, term " center ", " transverse direction ", "upper", "lower", "left", "right",
The orientation or positional relationship of the instructions such as "vertical", "horizontal", "top", "bottom", "inner", "outside" be orientation based on the figure or
Positional relationship is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must
There must be specific orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.In addition, art
Language " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance or implicitly indicate institute
The quantity of the technical characteristic of instruction." first " is defined as a result, the feature of " second " can explicitly or implicitly include one
Or more this feature.In the description of the present invention, unless otherwise indicated, the meaning of " plurality " is two or more.
In addition, term " includes " and its any deformation, it is intended that cover and non-exclusive include.
The present invention will be further explained below with reference to the attached drawings and specific examples.
Embodiment one, as shown in Figs 1-4, a kind of cell climbing sheet grabber according to an embodiment of the present invention, including hollow
Body 1, push rod 2 and cell climbing sheet 3, the hollow cylinder 1 are internally connected with the push rod 2, and the push rod 2 with it is described hollow
1 dismantling connection of cylinder, the diameter of the bottom end of the hollow cylinder 1 are less than the diameter on the top of the hollow cylinder 1, and it is described in
The bottom end of empty cylinder 1 is pyramidal structure, and the bottom end of the hollow cylinder 1 is fixed with sucker 4, the surface one of the hollow cylinder 1
Side offers high-order limit hole 5 and low level limit hole 6, and top a side of the hollow cylinder 1 is connected with by pressure arm 7, institute
The interior bottom portion for stating hollow cylinder 1 is fixed with reset spring 8, and the bottom end of the push rod 2 is fixed with suitable with 1 phase of hollow cylinder
The rubber plug 9 matched, the bottom side side of the push rod 2 is connected with elastic spacing device 10, and the elastic spacing device 10 passes through inclination
Bar 11 is connect with the push rod 2, the diameter of the elastic spacing device 10 and the high-order limit hole 5 and the low level limit hole 6
Diameter be adapted.
Embodiment two, as shown in Fig. 2, the high position limit hole 5, low level limit hole 6 and the pressure arm 7 of pressing are located in described
The same side of empty cylinder 1, and be located in same horizontal line.
Embodiment three, as shown in Figure 1, the rubber plug 9 and the hollow cylinder 1 are interference fitted, and the bottom end of the rubber plug 9
Diameter is less than the top end diameter of the rubber plug 9.
Example IV, as shown in Fig. 1 or 3, the top of the push rod 2 is fixed with pressing plate 12, and the pressing plate 12 is
Round pressing plate 12.
Embodiment five, as shown in Figs. 1-2, the vertical height by pressure arm 7 and the low level limit hole 6 are in same level
On face, the press surface that protrusion is fixed with by the bottom end of pressure arm 7 and a side of the close hollow cylinder 1.
Embodiment six, as shown in Figs. 1-2, the sucker 4 are rubber material.
Specific workflow: in use, first pressing push rod 2 is located at elastic spacing device 10 in low level limit hole 6, pressing is pushed away
When bar 2, elastic spacing device 10 is inclined by bar 11 and leads into inside hollow cylinder 1.Elastic spacing device 10 reaches low level limit hole 6
When bounce, the position of fixed push rod 2.Then sucker 4 is placed in one jiao of cell climbing sheet 3, sucker 4 and thin is firmly made to end
Born of the same parents' creep plate 3 is in close contact.Then it presses inward against by pressure arm 7, inwardly pushes elastic spacing device 10 by pressure arm 7, make elastic spacing device
10 retract inside hollow cylinder 1.Push rod 2 is pushed by reset spring 8, and reset spring 8 is compressed spring, drives rubber plug 9 upward
Movement.Since rubber plug 9 and hollow cylinder 1 are interference fit, when rubber plug 9 moves upwards, negative pressure is formed inside hollow cylinder 1.
At this point, sucker 4 and cell climbing sheet 3 are closely sealed.Lifting 3 grabber of cell climbing sheet upwards, can be by cell climbing sheet 3 and attaching surface point
From.Cell climbing sheet 3 is moved to after needing position, presses push rod 2 by preceding method, elastic spacing device 10 is made to reach low level limit
Hole 6.At this point, negative pressure disappears in hollow cylinder 1, cell climbing sheet 3 can be separated with 3 grabber of cell climbing sheet.
In conclusion by means of above-mentioned technical proposal of the invention, 3 grabber of cell climbing sheet of the invention can be light
Easily, cell climbing sheet 3 is quickly grabbed, it is related to carry out because of the probability that misoperation causes creep plate broken when reducing crawl creep plate
Experiment is provided convenience；The configuration of the present invention is simple, it is easy to operate, it is more convenient for people to use and cell climbing sheet 3 is grabbed
It takes.
The technical personnel in the technical field can readily realize the present invention with the above specific embodiments,.But it answers
Work as understanding, the present invention is not limited to above-mentioned specific embodiments.On the basis of the disclosed embodiments, the technical field
Technical staff can arbitrarily combine different technical features, to realize different technical solutions.
Claims (6)
1. a kind of cell climbing sheet grabber, which is characterized in that described including hollow cylinder (1), push rod (2) and cell climbing sheet (3)
Hollow cylinder (1) is internally connected with the push rod (2), and the push rod (2) and the hollow cylinder (1) dismantling connection, institute
State the bottom end of hollow cylinder (1) diameter be less than the hollow cylinder (1) top diameter, and the hollow cylinder (1)
Bottom end is pyramidal structure, and the bottom end of the hollow cylinder (1) is fixed with sucker (4), surface a side of the hollow cylinder (1)
High-order limit hole (5) and low level limit hole (6) are offered, top a side of the hollow cylinder (1) is connected with by pressure arm
(7), the interior bottom portion of the hollow cylinder (1) is fixed with reset spring (8), the bottom end of the push rod (2) be fixed with it is described
The compatible rubber plug (9) of hollow cylinder (1), the bottom side side of the push rod (2) are connected with elastic spacing device (10), and described
Elastic spacing device (10) is connect by tilting bar (11) with the push rod (2), the diameter of the elastic spacing device (10) with it is described
The diameter of high-order limit hole (5) and the low level limit hole (6) is adapted.
2. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the high position limit hole (5), low level
Limit hole (6) and the same side for being located at the hollow cylinder (1) by pressure arm (7), and be located in same horizontal line.
3. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the rubber plug (9) with it is described hollow
Cylinder (1) interference fit, and the bottom diameter of the rubber plug (9) is less than the top end diameter of the rubber plug (9).
4. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the top of the push rod (2) is fixed
Have pressing plate (12), and the pressing plate (12) is round pressing plate (12).
5. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the vertical height by pressure arm (7)
Degree and the low level limit hole (6) are described by the bottom end of pressure arm (7) and close to the hollow cylinder (1) in same level
A side be fixed with protrusion press surface.
6. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the sucker (4) is rubber material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910223995.0A CN109943466A (en) | 2019-03-22 | 2019-03-22 | A kind of cell climbing sheet grabber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910223995.0A CN109943466A (en) | 2019-03-22 | 2019-03-22 | A kind of cell climbing sheet grabber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109943466A true CN109943466A (en) | 2019-06-28 |
Family
ID=67011391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910223995.0A Pending CN109943466A (en) | 2019-03-22 | 2019-03-22 | A kind of cell climbing sheet grabber |
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| Country | Link |
|---|---|
| CN (1) | CN109943466A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113640089A (en) * | 2021-09-17 | 2021-11-12 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
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| US20060253083A1 (en) * | 2005-04-26 | 2006-11-09 | Wenjie Liu | Cylindrical pushrod for needle-retracting safety injector and the needle-retraction controllable safety injector |
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| CN109335274A (en) * | 2018-12-09 | 2019-02-15 | 李宗鲜 | A kind of storage of cell climbing sheet, taking device |
| CN210103903U (en) * | 2019-03-22 | 2020-02-21 | 华东医院 | Cell slide grabber |
-
2019
- 2019-03-22 CN CN201910223995.0A patent/CN109943466A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060253083A1 (en) * | 2005-04-26 | 2006-11-09 | Wenjie Liu | Cylindrical pushrod for needle-retracting safety injector and the needle-retraction controllable safety injector |
| CN103345054A (en) * | 2013-07-21 | 2013-10-09 | 薛峰 | Cover glass sucking-holding device |
| CN103908711A (en) * | 2014-04-14 | 2014-07-09 | 林新颖 | Puncture combined-type syringe |
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| CN104789465A (en) * | 2015-04-16 | 2015-07-22 | 辽宁医学院 | Experimental device for detecting cell migration |
| CN205951551U (en) * | 2016-05-31 | 2017-02-15 | 常州信息职业技术学院 | Automatic pen |
| CN106865229A (en) * | 2017-03-14 | 2017-06-20 | 京东方科技集团股份有限公司 | Adsorbent equipment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113640089A (en) * | 2021-09-17 | 2021-11-12 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
| CN113640089B (en) * | 2021-09-17 | 2022-02-08 | 北京博纳致恒科技有限责任公司 | Cell immunohistochemical staining device |
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