CN210103903U

CN210103903U - Cell slide grabber

Info

Publication number: CN210103903U
Application number: CN201920372648.XU
Authority: CN
Inventors: 王万晨; 陈亮; 刘天一; 贾传龙; 秦登科
Original assignee: Huadong Hospital
Current assignee: Huadong Hospital
Priority date: 2019-03-22
Filing date: 2019-03-22
Publication date: 2020-02-21
Anticipated expiration: 2029-03-22

Abstract

The utility model discloses a cell climbing piece grabber, including the cavity barrel, push rod and cell climbing piece, the internal connection of cavity barrel has the push rod, and the push rod is dismantled with the cavity barrel and is connected, the bottom mounting of cavity barrel has the sucking disc, the spacing hole of high-order and the spacing hole of low-order have been seted up to the surface side of cavity barrel, the top side of cavity barrel is connected with the arm of pressing, the inside bottom of cavity barrel is fixed with reset spring, the bottom mounting of push rod have with the plug of cavity barrel looks adaptation, the bottom side of push rod is connected with elastic limiter, and elastic limiter passes through the lifter and is connected with the push rod, elastic limiter's diameter and the diameter looks adaptation in the spacing hole of high-order and the spacing hole of low-order. Has the advantages that: the utility model discloses a cell climbing piece grabber can be easily, quick snatch the cell climbing piece, because of the broken probability of misoperation results in the climbing piece when reducing to snatch the climbing piece, facilitates for carrying out relevant experiment.

Description

Cell slide grabber

Technical Field

The utility model relates to a cell climbing piece's transfer and the apparatus technical field of separation relate to a cell climbing piece grabber particularly.

Background

The cell slide-climbing technology is that a slide is soaked in a cell culture medium, cells grow on the slide, and the cell slide-climbing technology is mainly used for histology, immunohistochemistry, in-situ hybridization and the like. The slide used in the technology is also called a cell slide, and the surface is specially treated generally, so that the slide is more suitable for the adhesion of adherent cells. The thickness of the slide is 0.17mm, the diameter is 8mm/14mm/20mm/25mm, and the slide is respectively suitable for 48-hole cell culture plates/24-hole cell culture plates/12-hole cell culture plates/6-hole cell culture plates. The traditional operation steps are as follows:

1. and (5) disinfecting the cell slide.

2. 1 drop of culture medium is dropped into each hole of the culture plate, and then the climbing sheet is placed on the drop and is compressed (the climbing sheet is adsorbed on the culture plate by the action of surface tension).

3. And (3) digesting, centrifuging and resuspending the conventional cells, adding the blown cell suspension to each climbing sheet respectively, or directly inoculating the conventional cells to a culture hole to allow the cells to grow adherent.

4. Cell culture fluid was aspirated and washed three times with PBS, fixed with 4% paraformaldehyde or other fixative.

5. After the cell slide is made, the slide is carefully taken out by using tweezers for subsequent detection.

Among them, the adherent climbing sheets are difficult to be taken out by using a conventional small forceps, and the climbing sheets are easy to be crushed when being taken out, which is also the biggest defect of the method. At present, a method or an apparatus for simply and safely grabbing the climbing film is lacked

An effective solution to the problems in the related art has not been proposed yet.

SUMMERY OF THE UTILITY MODEL

The technical task of the utility model is to above not enough, provide a cell slide grabber, solve above-mentioned problem.

The technical scheme of the utility model is realized like this:

a cell climbing sheet grabber, which comprises a hollow cylinder body, a push rod and a cell climbing sheet, wherein the push rod is connected inside the hollow cylinder body, the push rod is detachably connected with the hollow cylinder body, the diameter of the bottom end of the hollow cylinder body is smaller than that of the top end of the hollow cylinder body, the bottom end of the hollow cylinder body is of a conical structure, a sucking disc is fixed at the bottom end of the hollow cylinder body, a high-position limiting hole and a low-position limiting hole are formed in one side edge of the surface of the hollow cylinder body, a pressing arm is connected to one side edge of the top end of the hollow cylinder body, a return spring is fixed at the bottom of the interior of the hollow cylinder, a rubber plug matched with the hollow cylinder is fixed at the bottom end of the push rod, an elastic stopper is connected with one side edge of the bottom of the push rod, and the elastic limiting stopper is connected with the push rod through an inclined rod, and the diameter of the elastic limiting stopper is matched with the diameters of the high-position limiting hole and the low-position limiting hole.

Furthermore, the high-position limiting hole, the low-position limiting hole and the pressing arm are located on the same side of the hollow cylinder and on the same horizontal line.

Further, the rubber plug is in interference fit with the hollow cylinder, and the diameter of the bottom end of the rubber plug is smaller than that of the top end of the rubber plug.

Furthermore, a pressing plate is fixed at the top end of the push rod, and the pressing plate is a circular pressing plate.

Furthermore, the vertical height of the pressing arm and the low-position limiting hole are on the same horizontal plane, and a convex pressing surface is fixed at the bottom end of the pressing arm and on one side edge close to the hollow cylinder.

Furthermore, the sucking disc is made of rubber.

The utility model has the advantages that:

1. the utility model discloses a cell climbing piece grabber can be easily, quick snatch the cell climbing piece, because of the broken probability of misoperation results in the climbing piece when reducing to snatch the climbing piece, facilitates for carrying out relevant experiment.

2. The utility model discloses simple structure, convenient operation makes things convenient for people's use more and snatchs the cell slide.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic view of a cell slide grabber according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a cell slide according to an embodiment of the present invention;

fig. 3 is a schematic structural view of a hollow cylinder according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of a push rod according to an embodiment of the present invention.

In the figure:

1. a hollow cylinder; 2. a push rod; 3. cell climbing sheets; 4. a suction cup; 5. a high-position limiting hole; 6. a low position limiting hole; 7. pressing the arm; 8. a return spring; 9. a rubber plug; 10. an elastic stopper; 11. an inclined lever; 12. and pressing the plate.

Detailed Description

In the description of the present invention, it is to be understood that the terms "center", "lateral", "up", "down", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified. Furthermore, the term "comprises" and any variations thereof is intended to cover non-exclusive inclusions.

The invention will be further explained with reference to the drawings and the specific embodiments.

Embodiment one, as shown in fig. 1 to 4, a cell slide grabber according to an embodiment of the present invention includes a hollow cylinder 1, a push rod 2 and a cell slide 3, wherein the interior of the hollow cylinder 1 is connected with the push rod 2, the push rod 2 is detachably connected with the hollow cylinder 1, the diameter of the bottom end of the hollow cylinder 1 is smaller than the diameter of the top end of the hollow cylinder 1, the bottom end of the hollow cylinder 1 is of a conical structure, a suction cup 4 is fixed at the bottom end of the hollow cylinder 1, a high position limiting hole 5 and a low position limiting hole 6 are formed at one side of the surface of the hollow cylinder 1, a pressing arm 7 is connected with one side of the top end of the hollow cylinder 1, a return spring 8 is fixed at the bottom of the hollow cylinder 1, a rubber plug 9 adapted to the hollow cylinder 1 is fixed at the bottom end of the push rod 2, an elastic stopper 10 is connected with the bottom of the push rod 2, and the elastic limiter 10 is connected with the push rod 2 through an inclined rod 11, and the diameter of the elastic limiter 10 is matched with the diameters of the high-position limiting hole 5 and the low-position limiting hole 6.

In the second embodiment, as shown in fig. 2, the high-position limiting hole 5, the low-position limiting hole 6 and the pressing arm 7 are located on the same side of the hollow cylinder 1 and on the same horizontal line.

In the third embodiment, as shown in fig. 1, the rubber plug 9 is in interference fit with the hollow cylinder 1, and the diameter of the bottom end of the rubber plug 9 is smaller than the diameter of the top end of the rubber plug 9.

In the fourth embodiment, as shown in fig. 1 or 3, a pressing plate 12 is fixed to a tip of the push rod 2, and the pressing plate 12 is a circular pressing plate 12.

In the fifth embodiment, as shown in fig. 1-2, the vertical height of the pressing arm 7 is on the same horizontal plane as the low-position limiting hole 6, and a convex pressing surface is fixed at the bottom end of the pressing arm 7 and on a side close to the hollow cylinder 1.

In a sixth embodiment, as shown in fig. 1-2, the suction cup 4 is made of rubber.

The specific working process is as follows: when the elastic limiting device is used, the push rod 2 is pressed firstly, so that the elastic limiting device 10 is located in the low-position limiting hole 6, and when the push rod 2 is pressed, the elastic limiting device 10 is guided by the inclined rod 11 to enter the hollow cylinder body 1. The elastic limiting device 10 bounces when reaching the low position limiting hole 6, and the position of the push rod 2 is fixed. Then the suction cup 4 is placed at one corner of the cell slide 3, and the suction cup 4 and the cell slide 3 are tightly contacted with each other by a slight downward force. Then, the pressing arm 7 is pressed inward, and the pressing arm 7 pushes the elastic stopper 10 inward, so that the elastic stopper 10 is retracted into the hollow cylinder 1. The push rod 2 is pushed by a return spring 8, and the return spring 8 is a compression spring and drives the rubber plug 9 to move upwards. Because the rubber plug 9 and the hollow cylinder body 1 are in interference fit, when the rubber plug 9 moves upwards, negative pressure is formed inside the hollow cylinder body 1. At this time, the suction plate 4 is in close contact with the cell slide 3. The grabber of the cell slide 3 is lifted upwards, and the cell slide 3 can be separated from the attachment surface. After the cell climbing sheet 3 is moved to a desired position, the push rod 2 is pressed according to the method, so that the elastic stopper 10 reaches the low position limiting hole 6. At this time, the negative pressure in the hollow cylinder 1 disappears, and the cell climbing sheet 3 can be separated from the grabber of the cell climbing sheet 3.

In conclusion, with the aid of the technical scheme of the utility model, the cell slide 3 grabber of the utility model can easily and quickly grab the cell slide 3, reduce the probability of slide breakage caused by misoperation when grabbing the slide, and provide convenience for developing related experiments; the utility model discloses simple structure, convenient operation makes things convenient for people's use more and snatchs cell slide 3.

Through the above detailed description, the person skilled in the art can easily realize the present invention. It should be understood, however, that the intention is not to limit the invention to the particular embodiments described. On the basis of the disclosed embodiments, a person skilled in the art can combine different technical features at will, thereby implementing different technical solutions.

Claims

1. The cell climbing sheet grabber is characterized by comprising a hollow cylinder body (1), a push rod (2) and a cell climbing sheet (3), wherein the push rod (2) is connected inside the hollow cylinder body (1), the push rod (2) is detachably connected with the hollow cylinder body (1), the diameter of the bottom end of the hollow cylinder body (1) is smaller than that of the top end of the hollow cylinder body (1), the bottom end of the hollow cylinder body (1) is of a conical structure, a sucker (4) is fixed at the bottom end of the hollow cylinder body (1), a high-position limiting hole (5) and a low-position limiting hole (6) are formed in one side edge of the surface of the hollow cylinder body (1), a pressing arm (7) is connected to one side edge of the top end of the hollow cylinder body (1), a reset spring (8) is fixed at the bottom inside of the hollow cylinder body (1), a rubber plug (9) matched with the hollow cylinder body (1) is fixed at the bottom end of the push rod (2), an elastic limiting stopper (10) is connected to one side edge of the bottom of the push rod (2), the elastic limiting stopper (10) is connected with the push rod (2) through an inclined rod (11), and the diameter of the elastic limiting stopper (10) is matched with the diameter of the high-position limiting hole (5) and the diameter of the low-position limiting hole (6).

2. The cell-slide grabber as claimed in claim 1, wherein the high position limiting hole (5), the low position limiting hole (6) and the pressing arm (7) are located on the same side of the hollow cylinder (1) and on the same horizontal line.

3. The cell-slide grabber as claimed in claim 1, wherein the rubber plug (9) is in interference fit with the hollow cylinder (1), and the diameter of the bottom end of the rubber plug (9) is smaller than the diameter of the top end of the rubber plug (9).

4. The cell-slide grabber as claimed in claim 1, wherein a pressing plate (12) is fixed to a top end of the push rod (2), and the pressing plate (12) is a circular pressing plate (12).

5. The cell-slide grabber as claimed in claim 1, wherein the vertical height of the pressing arm (7) is in the same horizontal plane as the low-position limiting hole (6), and a convex pressing surface is fixed at the bottom end of the pressing arm (7) and on one side close to the hollow cylinder (1).

6. The cell-slide grabber as recited in claim 1, wherein the suction cup (4) is made of rubber.