WO2012031493A1

WO2012031493A1 - Frame used for fixing array of high-density cell-attached coverslips in petri dish

Info

Publication number: WO2012031493A1
Application number: PCT/CN2011/075309
Authority: WO
Inventors: 李宏; 刘佳; 孔庆友; 孙媛
Original assignee: Li Hong; Liu Jia; Kong Qingyou; Sun Yuan
Priority date: 2010-09-06
Filing date: 2011-06-03
Publication date: 2012-03-15
Also published as: CN202214367U; CN102206582A; CN101974425A

Abstract

Provided is a frame used for fixing array of high-density cell-attached coverslips in a petri dish, which has cross arms (1). There are several horizontal bulges (3) on the cross arm (1) at intervals. The frame is simple and easy to be used. The bulges can fix coverslips between cross arms and bulges so as to prevent coverslips from floating, dislocating or moving away and improve efficiency of cell culture. Moreover, in order to use the frame only one time, the connecting point between the cross arm and the bulge is easily broken when the coverslip is taken out.

Description

[Name of invention made by ISA according to Rule 37.2] Shelf for fixing high-density cell climbing arrays in petri dishes

Technical field:

The invention relates to an auxiliary device for cell culture, in particular to a simple structure and convenient use. A high-density array of cell slide culture dishes that prevent slides from floating and moving with a slide holder.

Background technique:

With the continuous enrichment of life science research content, the technical indicators for analysis and observation using cell culture experimental samples are also increasing, and the fields covered are expanding. However, if the cultured cells of each culture differ in terms of growth pattern, cell and molecular biological characteristics, the results of the experiment may be biased. In the past, an improved in vitro cell culture method was generally adopted, that is, taking some appropriate sizes (such as 10mm × 20mm, thickness 0.14~0.17mm Cover slips, acid soaked and fully rinsed and autoclaved; place coverslips in culture dishes/plates, place regular cells in front of the tablets; when the cells are substantially overgrown on the coverslips, remove the sheets; Perform subsequent tests. Since the coverslips used are easy to move and overlap at the bottom of the smooth bottle, the growth conditions and area of the cells on each cover slip are different, and there is a problem that the experimental results are deviated due to the poor identity of the slides. . In order to solve this problem, the Chinese patent number is 200610047607.0 The invention patent entitled 'High-density array cell crawler culture device, storage device and experimental device' discloses a high-density array cell crawler culture device, the structure of which is There is a carrier plate, and a plurality of sequentially arranged grooves are arranged on the carrier plate. The depth of the groove is 0.3~0.4mm, and the groove has a slide piece which is matched with the shape of the bottom of the groove 2, and can be not only on a single culture dish. Prepared 50 The slugs of the left and right cells can ensure the identity of the climbing pieces. However, because there is currently no dedicated slide fixture, The slide placed in the groove sometimes floats under the action of a certain external force of the fluid or dislodges or moves due to vibration during transportation.

Summary of the invention:

The present invention is to solve the above technical problems existing in the prior art, and provides a simple structure and convenient use. A high-density array of cell slide culture dishes that prevent slides from floating and moving with a slide holder.

The technical solution of the present invention is: a slide holder for a high-density array type cell climbing culture dish, characterized in that: a cross bracing is provided, and a plurality of spaced protrusions are arranged in the horizontal direction of the cross bracing Start.

A plurality of vertical positioning holes are spaced apart from the cross bracing.

The width of the cross section of the cross bracing is 0.1~0.15mm, and the height of the cross section is 0.3~0.5mm The protrusion is located at an upper end of the cross brace.

The protrusion is triangular or circular, and is connected to the cross bracing, and has a thickness of 0.1 to 0.15 mm.

The protrusion is a thin wedge shape with an outer thickness.

There are connecting ribs between the cross braces.

At least two horizontally located limiting members are disposed on the outer edge of the grid formed by the cross braces and the connecting ribs.

The connecting rib is a ring that is connected to the ends of the plurality of struts.

The invention has the advantages of simple structure and convenient use, and the protrusions provided can The slide placed in the groove is fixed to effectively prevent the slide from floating, dislocating or moving, and the uniform and stable cell climbing can be obtained with high quality and quantity, further improving the working efficiency of the cell culture; especially the point of the protrusion and the cross bracing The connection method can be broken by the external force of the removal slide, thereby ensuring the property of the cell climbing culture device for one-time use.

BRIEF DESCRIPTION OF THE DRAWINGS:

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic view showing the structure of a first embodiment of the present invention.

Fig. 2 is a view showing the use effect of the embodiment 1 of the present invention.

Fig. 3 is a schematic structural view of Embodiment 2 of the present invention.

Fig. 4 is a view showing the use effect of the embodiment 2 of the present invention.

Figure 5 is a structural effect view of Embodiment 3 of the present invention.

Figure 6 is a structural effect view of Embodiment 4 of the present invention.

Figure 7 is a schematic view showing the structure of Embodiment 5 of the present invention.

Figure 8 is a schematic view showing the structure of Embodiment 6 of the present invention.

detailed description:

Specific embodiments of the present invention will be described below with reference to the accompanying drawings.

Example 1

As shown in FIG. 1 , a cross brace 1 is provided, and a plurality of spaced

protrusions

3 and 3 are arranged in the horizontal direction of the cross brace 1 It can be of various shapes and can be placed on one side or both sides of the horizontal support 1 in the horizontal direction.

The number of the cross braces 1, the number of the projections 3, and the spacing are all based on the high-density array cell crawler culture device. The groove size and quantity depend on it. When used, as shown in Figure 2, a long groove matching the cross brace 1 can be machined on the carrier plate of the high-density array cell slide culture device applied, and the cross bracing is installed during the installation. Placed in the long groove to ensure tightly mounted on the carrier plate of the high-density array cell slide culture device, the cross brace 1 is placed at the pitch of the groove 5, and the protrusion 3 is located above the groove 5 for fixing Groove 5 Inside the slide.

Example 2:

As shown in Fig. 3, as in the first embodiment, a cross brace 1 is provided, and a plurality of spaced projections are connected in the horizontal direction of the cross brace 1 The projections 3 may be of various shapes and may be disposed on one side or both sides of the horizontal support 1 in the horizontal direction. Different from Embodiment 1, a plurality of vertical positioning holes 5 are spaced apart from each other on the cross member 1.

The number of the cross braces 1, the projections 3, the number and spacing of the positioning holes 5 are all required to be based on the high-density array cell crawler culture device on the slide plate. The groove size and quantity depend on it. When used, as shown in Figure 4, a long groove matching the cross brace 1 can be machined on the carrier plate of the high-density array cell crawler culture device applied and the positioning hole can be machined in the groove. 5 Matching studs, when installing the cross braces 1 in the long groove, the positioning holes 5 are placed on the studs, and then the studs are hot-melted. Therefore, it is ensured that the integrated mounting is fixed on the carrier plate of the high-density array cell crawler culture device. The cross braces 1 are placed at the pitch of the grooves 5, and the projections 3 are located above the grooves 5 for fixing the slides in the grooves 5.

Example 3:

As shown in FIG. 5, a cross brace 1 is provided, and a plurality of spaced protrusions 3 are arranged in the horizontal direction of the cross brace 1 , and the protrusion 3 It can be various shapes, such as a thin wedge shape inside the outer thickness. The protrusion 3 may be disposed on one side or both sides of the horizontal support 1 in the horizontal direction, and the width of the cross section of the cross support 1 is 0.1 to 0.15 mm, and the height of the cross section is 0.3~0.5mm, the protrusion 3 is located at the upper end of the cross brace 1.

The installation method can be as in the embodiment 1. Due to the protrusion 3 and the cross support 1 For the point connection and the thin thickness, the external force when the slide is taken out can be broken, thereby ensuring the properties of the cell climbing culture device for one-time use.

Example 4:

As shown in Fig. 6, there are a plurality of cross braces 1 which are arranged parallel to each other and spaced apart, and there is a connecting rib 2 between the cross braces 1 in the cross braces 1 A plurality of spaced-apart triangular protrusions 3 are connected in the horizontal direction, and the protrusions 3 may be disposed on one side or both sides of the horizontal direction 1 in the horizontal direction, and the connecting ribs 2 may be connected to the plurality of horizontal lines as shown in FIG. Support 1 The ring of the endpoint. The spacing between the cross braces 1, the number and spacing of the projections 3 are all based on the high density array cell slide culture device on the slide plate. Depending on the size and number of grooves, it is ensured that after the compact mounting on the carrier plate of the high-density array cell culture device, the cross braces 1 are placed at the pitch of the grooves 5, and the projections 3 are located above the grooves 5 for Fixed groove 5 Inside the slide.

Example 5:

As shown in Figure 7:

The basic structure is the same as in the embodiment 4 except that the cross braces 1 and the connecting ribs 2 The outer edge of the frame is provided with at least two limiting members 4 (ribs or grooves) in the horizontal direction, which can be arranged on the cross braces 1 or / and the connecting ribs 2. The protrusion 3 is round, and the cross support 1 For point connection, the thickness is 0.1~0.15mm.

It can be processed and restrained on the inner side of the side wall of the culture dish where the carrier plate of the high-density array cell culture device is placed. Matching grooves or ribs for further positioning.

Example 6:

As shown in Figure 8, there are a plurality of cross braces 1 which are arranged parallel to each other and spaced apart. There is a connecting rib 2 between the cross braces 1 , in which the cross braces 1 The horizontal direction is connected with a plurality of spaced protrusions 3, and the protrusions 3 may be disposed on one side or both sides of the horizontal support 1 in the horizontal direction, and the connecting ribs 2 may be connected to the plurality of cross supports as shown in the figure 1 The vertical support between the other structures can be the same as in the embodiment 5.

The materials used in the examples 1~6 should meet the medical requirements.

Claims

1 . A high-density array type cell slide culture dish with a slide holder, characterized in that: a cross bracing (1) is provided, and the cross bracing (1) A plurality of spaced protrusions (3) are connected in the horizontal direction.

2 . The slide holder for a high-density array type cell slide culture dish according to claim 1, wherein the cross support (1) ) A plurality of vertical positioning holes ( 5 ) are arranged at intervals.

3 . The slide holder for a high-density array type cell slide culture dish according to claim 1, wherein a width of the cross section of the cross member (1) is 0.1~0.15mm, the height of the cross section is 0.3~0.5mm, and the protrusion (3) is located at the upper end of the cross bracing (1).

4 . A slide holder for a high-density array type cell slide culture dish according to claim 1 or 2 or 3, wherein: said protrusion (3) ) is a triangle or a circle, and is connected to the cross bracing ( 1 ) as a point, and has a thickness of 0.1 to 0.15 mm.

5 . A slide holder for a high-density array type cell slide culture dish according to claim 1 or 2 or 3, wherein: said protrusion (3) ) is a thin wedge in the outer thickness.

6 . A slide holder for a high-density array type cell slide culture dish according to claim 1 or 2 or 3, wherein: said cross support (1) There is a connecting rib ( 2 ) between.

7 . The slide holder for a high-density array type cell slide culture dish according to claim 6, wherein: the cross bracing (1) and the connecting rib (2) The outer edge of the frame is provided with at least two limiting members (4) in the horizontal direction.

8 . The slide holder for a high-density array type cell slide culture dish according to claim 7, wherein the connecting rib (2) is connected to a plurality of cross braces ( 1) The ring of the endpoint.