CN2440820Y

CN2440820Y - Cell culture dish

Info

Publication number: CN2440820Y
Application number: CN 00254681
Authority: CN
Inventors: 陆纲
Original assignee: Individual
Current assignee: Individual
Priority date: 2000-09-22
Filing date: 2000-09-22
Publication date: 2001-08-01
Anticipated expiration: 2010-09-22

Abstract

The utility model relates to a cell culture dish. A groove-shaped cell culture cavity is arranged on the center point, the height of the cell culture cavity is at least 2 mm, and the outer diameter of the cell culture cavity is matched with a groove which is arranged on a bracket. An upper cover with a hole in the center is arranged on the cell culture dish, two electrodes can be vertically inserted into the upper cover, and the inserting ends are mutually bent towards the horizontal direction in parallel, and are arranged on the bottom of the cell culture cavity. The design can be directly arranged on the bracket, which has good stability and can not slide, and the bottom area is large. The goal that fluorescent probes are saved is achieved and cells grow well. Cell culture fluid which is added quantitatively can not flow out, the concentration is accurate, and the experiment precision is high. The utility model can also be matched with cell stimulating electrodes to use.

Description

Tissue Culture Dish

The utility model relates to a kind of cell culture apparatus, especially relates to a kind of Tissue Culture Dish.

At present, the fluorescent probe special cell culture dish that medical circle is generally used, be to be 35mm at a diameter, the hole, garden that it is 10.5mm that a diameter is dug in the bottom of the bulge that plastics are made, it is thick to stick with glue a 0.15mm again, size is the cover glass of 18.0mm * 18.0mm, promptly form one and surrounded by glass and plastics, diameter is 10.5mm, and the degree of depth is the cylindrical of 1.0mm, cell cultures is in cylinder, and the fluorescent probe mark also carries out therein.There is following shortcoming in it: (1) this product will place on the Stage microscope of fluorescent microscope or laser confocal microscope when observation, but its diameter is littler than Stage microscope, can not directly place on the Stage microscope, need do a size and the similar holder of Stage microscope, this product be placed in the holder just can observe then; (2) this product is smoothly placed on the carriage of Stage microscope, along with moving of Stage microscope easily slided, and easier moving it when wherein adding medicine and experimentize, thereby the previously selected visual field is changed, have to readjust; (3) floorage of this product is less, is 85.0mm ², in general cell in less environment growth phase to relatively poor; And its degree of depth is more shallow, is 1.0mm, and quantitatively the cell culture fluid that adds very easily flows out, and when wherein adding medicine, calculating good drug level in advance will change because liquid flows out, and influences experimental precision.

The purpose of this utility model provides a kind of simple in structure, good stability, and the experimental precision height not only can be used for fluorescent probe, also can be used for the Tissue Culture Dish of cell electricity irritation experiment.

The utility model Tissue Culture Dish, comprise the round cell culture dish, wherein the center in described round cell culture dish is provided with a groove shape cell culture chamber, and the height of described cell culture chamber is 2.0mm at least, and the external diameter of described Tissue Culture Dish matches with groove on the carriage.

The utility model Tissue Culture Dish, comprise outer ware and cover glass, wherein ware inside is equipped with the interior ware that a bottom bonds together with it coaxially outside described, there is a through hole center of ware and interior ware outside described, the sticking described cover glass in described through hole, the bottom of ware outside described, described through hole and cover glass form cell culture chamber together, and the height of described cell culture chamber is 2.0mm at least.The external diameter of described outer ware matches with groove on the carriage.

The further improvements of the utility model Tissue Culture Dish are that cell culture chamber is square, and its length of side is 12mm-18mm.

Another improvements of the utility model Tissue Culture Dish are that the diameter of outer ware is 57.0mm, and the diameter of interior ware is 35mm.

An improvement part again of the utility model Tissue Culture Dish is that described Tissue Culture Dish is provided with center foraminous loam cake, on described, cover and two electrodes of the vertical insertion in the square through hole diagonal lines corresponding section of Tissue Culture Dish, the insertion end of described two electrodes is parallel to each other to the horizontal direction bending, and be positioned at the bottom of cell culture chamber, the other end of two electrodes links to each other with lead.

The another improvements of the utility model Tissue Culture Dish are that described electrode is that platinum filament is made.

Advantage of the present utility model and positively effect are because the size and the Stage microscope of the design of this Tissue Culture Dish are similar, can directly place on the Stage microscope, good stability, be difficult for sliding, when adding medicine, also can not slide, its floorage is bigger than like product, both reached the purpose of saving fluorescent probe, and cell is owing to have relatively large growing environment, growth good; Because the bottom of Tissue Culture Dish is two-layer, has deepened the degree of depth of central square through hole, quantitatively the cell culture fluid that adds is difficult for flowing out, thereby after the medicine adding, concentration is accurate, the experimental precision height; Because the bottom of Tissue Culture Dish is a square opening, can with the supporting use of cytositimulation electrode.

Other details of the present invention and characteristics can be cheer and bright by reading the embodiment hereinafter encyclopaedize in conjunction with the accompanying drawings, wherein:

Fig. 1 is the perspective view of the utility model Tissue Culture Dish first embodiment;

Fig. 2 is the sectional view of the utility model Tissue Culture Dish first embodiment;

Fig. 3 is the perspective view of the utility model Tissue Culture Dish loam cake;

Fig. 4 is the sectional view of the utility model Tissue Culture Dish second embodiment;

Fig. 5 places use synoptic diagram on the fluorescent microscope for the utility model Tissue Culture Dish.

As Fig. 1, shown in 2, the utility model Tissue Culture Dish 1 comprises outer ware 2 and cover glass 3, and wherein ware 2 inside are equipped with the interior ware 4 that a bottom bonds together with it coaxially outside, there is a through hole 5 center of ware 2 and interior ware 4 outside, through hole 5 can be circular, the square arbitrary shape that waits, the sticking described cover glass 3 in the described through hole in the bottom of ware 25 places outside, through hole 5 and cover glass 3 form cell culture chamber 6 together, and the height of described cell culture chamber 6 is 2.0mm at least.The external diameter of described outer ware 2 matches with groove (not drawing among the figure) on the carriage.In the present embodiment, the diameter of outer ware is 57.0mm, and the diameter of interior ware is 35mm.

When needs are done cell electricity irritation experiment, use the Tissue Culture Dish that has square opening, and the length of side of square opening is 12mm-18mm.And should cover loam cake 7, as shown in Figure 3, the center of loam cake 7 is provided with the hole, on loam cake 7, vertically insert two electrodes 8,9 with the square opening diagonal lines corresponding section of Tissue Culture Dish 1, the insertion end of described two electrodes 8,9 is parallel to each other to the horizontal direction bending, and be positioned at the bottom of cell culture chamber 6, the other end of two electrodes 8,9 links to each other with lead 10.

As shown in Figure 4, be the utility model Tissue Culture Dish second embodiment, comprise round cell culture dish 1`, wherein the center in described round cell culture dish 1` is provided with a groove shape cell culture chamber 6, the height of described cell culture chamber 6 is 2.0mm at least, and the external diameter of described Tissue Culture Dish 1` matches with groove on the carriage.In the present embodiment, the shape of cell culture chamber 6 can be circular, and square other arbitrary shapes that waits when doing cell electricity irritation experiment, use to have the Tissue Culture Dish of square opening, and cover and the identical loam cake 7 of above-mentioned loam cake 7 structures.

As shown in Figure 5, use synoptic diagram for the utility model Tissue Culture Dish, earlier cell inoculation is grown in the cell culture chamber 6 of Tissue Culture Dish 1, regularly change nutrient solution, treat that cell grows to proper time, in Tissue Culture Dish, carry out the fluorescent probe mark, then, Tissue Culture Dish 1 is placed on the Stage microscope of fluorescent microscope 11 and observe, when needs cytositimulation electrode is tested, cover loam cake 7, electrode 8 on the loam cake 7, in 9 cell culture chamber 6 that will be inserted in the Tissue Culture Dish 1, its upper conductor 10 is connected with electric stimulating instrument 12, can add medicine in cell by the aperture at loam cake 7 centers as required, because the electrode trend is for parallel, thereby the electric field of producing is uniform, meet the needs of experiment, thereby realized its inner variation that takes place when being subjected to electricity irritation of real-time monitored cell, resulting result is more accurate, reliably.

Claims

1, a kind of Tissue Culture Dish, comprise the round cell culture dish, it is characterized in that the center in described round cell culture dish is provided with a groove shape cell culture chamber, the height of described cell culture chamber is 2.0mm at least, and the external diameter of described Tissue Culture Dish matches with groove on the carriage.

2, a kind of Tissue Culture Dish, comprise outer ware and cover glass, it is characterized in that ware inside is equipped with the interior ware that a bottom bonds together with it coaxially outside described, there is a through hole center of ware and interior ware outside described, the sticking described cover glass in described through hole, the bottom of ware outside described, described through hole and cover glass form cell culture chamber together, and the height of described cell culture chamber is 2.0mm at least, and the external diameter of described outer ware matches with groove on the carriage.

3, Tissue Culture Dish according to claim 1 and 2 is characterized in that cell culture chamber is square, and its length of side is 12mm-18mm.

4, Tissue Culture Dish according to claim 3, it is characterized in that described Tissue Culture Dish is provided with center foraminous loam cake, on described, cover and two electrodes of the vertical insertion in the square through hole diagonal lines corresponding section of Tissue Culture Dish, the insertion end of described two electrodes is parallel to each other to the horizontal direction bending, and be positioned at the bottom of cell culture chamber, the other end of two electrodes links to each other with lead.

5, Tissue Culture Dish according to claim 4, the diameter that it is characterized in that described outer ware is 57.0mm, the diameter of interior ware is 35mm.

6, Tissue Culture Dish according to claim 5 is characterized in that described electrode is that platinum filament is made.