CN101880628A - Cell culture dish for constant-volume perfusion - Google Patents

Cell culture dish for constant-volume perfusion Download PDF

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Publication number
CN101880628A
CN101880628A CN 201010226356 CN201010226356A CN101880628A CN 101880628 A CN101880628 A CN 101880628A CN 201010226356 CN201010226356 CN 201010226356 CN 201010226356 A CN201010226356 A CN 201010226356A CN 101880628 A CN101880628 A CN 101880628A
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China
Prior art keywords
liquid
import
drain pipe
perfusion
culture dish
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Granted
Application number
CN 201010226356
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Chinese (zh)
Other versions
CN101880628B (en
Inventor
赵玉峰
王莉
陈健康
于军
王跃民
刘利兵
刘芳娥
曲萍
杨芳
黄小军
周幸春
铁茹
刘楠
田菲
梁向艳
张学策
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Fourth Military Medical University FMMU
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Fourth Military Medical University FMMU
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Priority to CN 201010226356 priority Critical patent/CN101880628B/en
Publication of CN101880628A publication Critical patent/CN101880628A/en
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Publication of CN101880628B publication Critical patent/CN101880628B/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a cell culture dish for constant-volume perfusion, which comprises an outer box and is characterized in that an inner box is sleeved in the outer box; the inner box takes a shape of an inverted bamboo hat; channels are respectively arranged between both sides of the upper circular truncated cone surface and the lower conical surface; one channel is a liquid inlet pipe and is of an L-shape; the other channel is a liquid outlet pipe and is of a U-shape; an inlet of the liquid inlet pipe is communicated with a perfusion administration device; an outlet of the liquid outlet pipe is communicated with a negative pressure suction device; an outlet of the liquid inlet pipe on the conical surface is lower than an inlet of the liquid outlet pipe on the conical surface; and the inlet of the liquid outlet pipe on the conical surface is upward.

Description

A kind of Tissue Culture Dish of constant-volume perfusion
Technical field
The present invention relates to a kind of medical experiment vessel, but particularly a kind of Tissue Culture Dish of constant-volume perfusion administration.
Background technology
In function assessment research, it is multiple medical experiment method commonly used that the viable cell of cultivating is carried out the perfusion administration.The requirement of perfusion administration be convenient, simple, fast, economize medicine etc.At present the diameter that culturing cell is commonly used be the disc culture dish of 35mm because the amount of liquid of being contained in the ware big (about 3ml), it is generally bad to change the liquid effect, can not reach the effect of fully changing liquid and saving medicine fast.
Summary of the invention
The culture dish that the purpose of this invention is to provide a kind of very convenient perfusion, can realize fully without any need for other culture dish supplementary units, fast, the task of low consumption of the liquid perfusion.
For reaching above purpose, the present invention takes following technical scheme to be achieved:
A kind of Tissue Culture Dish of constant-volume perfusion comprises outer box, it is characterized in that, is with an inner box in the described outer box, and this inner box is an inverted-shaped hat, and its round table surface both sides, top respectively are provided with a pipeline to the lower cone face, and one of them is a liquid-inlet pipe, is the L type; Another is a drain pipe, is the U type; The import UNICOM perfusion doser of liquid-inlet pipe; The outlet UNICOM negative pressure suction device of drain pipe; Liquid-inlet pipe is lower than the import of drain pipe on cone surface in the outlet on the cone surface, and the import of drain pipe on cone surface up.
In the such scheme, the import of described drain pipe on cone surface is provided with two at least along the cone surface bus, and while UNICOM's drain pipe, when being positioned at the import step of top, with the import sealing of below.
Can be provided with the circulation heat insulation liquid between described inner box and the outer box.
Advantage of the present invention is:
1, reduces the internal volume of culture dish, thereby effectively reduce liquid measure, the purpose that reaches fast, fully changes liquid and save medicine.
2, the inner box lower taper increases opening, helps pair cell and carries out record, carries out the operation of unicellular recording electrode when clamping as cell patch.
3, liquid-inlet pipe is lower than the import of drain pipe on cone surface in the outlet on the cone surface, has guaranteed the facility of perfusion and the thorough mixing of liquid.
4, adopt on L type liquid-inlet pipe, U type drain pipe and the drain pipe cone surface import up, liquid level remains unchanged when only utilizing structural ingenious design just can guarantee perfusion, thereby has reduced the situation that liquid dries up in interference and the culture dish.
5, by adjusting the height of drain pipe import, liquid level liftable when making perfusion, when satisfying the different sorts cell cultures to the volumetrical needs.
Description of drawings
Fig. 1 is a structure iron of the present invention.
Fig. 2 is the vertical view of Fig. 1.
Among Fig. 1 Fig. 2: 1, drain pipe outlet; 2, drain pipe; 3, heat insulation liquid; 4, culturing cell; 5, drain pipe import; 6, perfusate; 7, liquid-inlet pipe outlet; 8, the conical surface; 9, liquid-inlet pipe; 10, outer box; 11, inner box; 12, liquid-inlet pipe import.
Embodiment
The present invention is described in further detail below in conjunction with drawings and the specific embodiments.
As shown in Figure 1 and Figure 2, a kind of Tissue Culture Dish of constant-volume perfusion comprises outer box 10, be with an inner box 11 in the outer box 10, this inner box is an inverted-shaped hat, and its round table surface both sides, top respectively are provided with a pipeline to lower cone face 8, one of them is a liquid-inlet pipe 9, is the L type; Another is a drain pipe 2, is the U type; Import 12 UNICOM's perfusion dosers of liquid-inlet pipe; Outlet 1 UNICOM's negative pressure suction device of drain pipe; Liquid-inlet pipe is lower than the import 5 of drain pipe on cone surface in the outlet on the cone surface 7, and the import 5 of drain pipe on cone surface up.Pipe diameter is 2mm.
The import 5 of drain pipe on cone surface is provided with three along the cone surface bus, and while UNICOM's drain pipe, adjust the height of drain pipe import, when being positioned at the import step of top, import sealing with the below, liquid level liftable when making perfusion, when satisfying the different sorts cell cultures to the volumetrical needs.Can be provided with circulation heat insulation liquid 3 between inner box 11 and the outer box 10.
Principle of the present invention is to reduce actual culture area and nutrient solution space, and liquid-inlet pipe import 12 perfusion feed liquors, drain pipe export 1 vacuum suction sucking-off liquid.The pipe U-shaped design of fluid has prevented the excessive influence to liquid level of negative pressure, can keep liquid level in constant level.

Claims (3)

1. the Tissue Culture Dish of a constant-volume perfusion comprises outer box, it is characterized in that, is with an inner box in the described outer box, and this inner box is an inverted-shaped hat, and its round table surface both sides, top respectively are provided with a pipeline to the lower cone face, and one of them is a liquid-inlet pipe, is the L type; Another is a drain pipe, is the U type; The import UNICOM perfusion doser of liquid-inlet pipe; The outlet UNICOM negative pressure suction device of drain pipe; Liquid-inlet pipe is lower than the import of drain pipe on cone surface in the outlet on the cone surface, and the import of drain pipe on cone surface up.
2. the Tissue Culture Dish of constant-volume perfusion as claimed in claim 1, it is characterized in that the import of described drain pipe on cone surface is provided with two at least along the cone surface bus, and while UNICOM's drain pipe, when being positioned at the import step of top, with the import sealing of below.
3. the Tissue Culture Dish of constant-volume perfusion as claimed in claim 1 is characterized in that, is provided with the circulation heat insulation liquid between described inner box and the outer box.
CN 201010226356 2010-07-14 2010-07-14 Cell culture dish for constant-volume perfusion Expired - Fee Related CN101880628B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201010226356 CN101880628B (en) 2010-07-14 2010-07-14 Cell culture dish for constant-volume perfusion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201010226356 CN101880628B (en) 2010-07-14 2010-07-14 Cell culture dish for constant-volume perfusion

Publications (2)

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CN101880628A true CN101880628A (en) 2010-11-10
CN101880628B CN101880628B (en) 2012-05-30

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113717840A (en) * 2021-08-12 2021-11-30 汕头大学医学院 U-shaped tube rapid drug delivery system and control method

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552321A (en) * 1993-08-24 1996-09-03 Bioptechs Inc. Temperature controlled culture dish apparatus
US6146881A (en) * 1997-09-24 2000-11-14 Steffen Hering Device and method for handling objects in a liquid bath
CN2440820Y (en) * 2000-09-22 2001-08-01 贾弘褆 Cell culture dish
US20090191631A1 (en) * 2006-07-10 2009-07-30 Reimhard Bornemann 3-D petri-dish for the culture and studies of cells
CN101597565A (en) * 2009-07-10 2009-12-09 曾勇 Tissue Culture Dish
CN201378218Y (en) * 2009-03-13 2010-01-06 周晋 Dual-purpose dish for culture and perfusion
CN201459140U (en) * 2009-07-27 2010-05-12 国家纳米科学中心 Parallel-plate flowing small chamber with tiny ridges

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552321A (en) * 1993-08-24 1996-09-03 Bioptechs Inc. Temperature controlled culture dish apparatus
US6146881A (en) * 1997-09-24 2000-11-14 Steffen Hering Device and method for handling objects in a liquid bath
CN2440820Y (en) * 2000-09-22 2001-08-01 贾弘褆 Cell culture dish
US20090191631A1 (en) * 2006-07-10 2009-07-30 Reimhard Bornemann 3-D petri-dish for the culture and studies of cells
CN201378218Y (en) * 2009-03-13 2010-01-06 周晋 Dual-purpose dish for culture and perfusion
CN101597565A (en) * 2009-07-10 2009-12-09 曾勇 Tissue Culture Dish
CN201459140U (en) * 2009-07-27 2010-05-12 国家纳米科学中心 Parallel-plate flowing small chamber with tiny ridges

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113717840A (en) * 2021-08-12 2021-11-30 汕头大学医学院 U-shaped tube rapid drug delivery system and control method

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Granted publication date: 20120530

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