CN204560518U - One Plants deep-layer liquid tissue culture vessel - Google Patents
One Plants deep-layer liquid tissue culture vessel Download PDFInfo
- Publication number
- CN204560518U CN204560518U CN201520170279.8U CN201520170279U CN204560518U CN 204560518 U CN204560518 U CN 204560518U CN 201520170279 U CN201520170279 U CN 201520170279U CN 204560518 U CN204560518 U CN 204560518U
- Authority
- CN
- China
- Prior art keywords
- sponge
- loose structure
- culture vessel
- layer liquid
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model relates to a Plants deep-layer liquid tissue culture vessel, platymiscium culture vessel technical field.The utility model is the plant deep-layer liquid tissue culture vessel after improving, comprise the container (2) of hyalosome structure, be provided with the top cover (1) of filtering microorganism parts, be placed in the sponge (4) of the loose structure of the bottom of container (2), be placed in the filter paper (3) needing setting depending on cultivation or do not arrange on the sponge (4) of loose structure, the sponge ring (6) needing setting depending on cultivation or do not arrange under being placed in the sponge (4) of loose structure, is placed in the air filter (5) on top cover (1); Wherein: the sponge (4) of loose structure is for which is provided with the sponge of the loose structure of a pit.Of the present utility modelly effectively to be: structure is simple, effectively can avoid the problem of vitrifying, lopsided seedling; Culture is more quick to the absorption of nutrient, and growth rate is faster, and culture efficiency is high, shortens incubation time.
Description
Technical field:
The utility model relates to a Plants deep-layer liquid tissue culture vessel, platymiscium culture vessel technical field.
Background technology:
The feature of current plant tissue culture technique is that labour intensity is large, automaticity is low, solid culture cost is high, and this is also the reason of restriction tissue cultures commercial application.Compared with solid culture medium, liquid nutrient medium has the superiority of its uniqueness, and first it provides more consistent condition of culture, balanced in nutrition, and nutrient is easy to be absorbed by explant.Although liquid nutrient medium has many advantages compared with solid culture medium, cultivate in the long-term liquid medium within of culture, there will be the problem of vitrifying, lopsided seedling.
Utility model content:
The purpose of this utility model is the deficiency overcoming prior art, and provides a kind of plant deep-layer liquid tissue culture vessel that can improve culture efficiency and quality.
To achieve these goals, the technical solution adopted in the utility model is:
The utility model is the improvement on existing culture vessel basis.Plant deep-layer liquid tissue culture vessel after improvement, comprise the container (2) of hyalosome structure, be provided with the top cover (1) of filtering microorganism parts, be placed in the sponge (4) of the loose structure of the bottom of container (2), be placed in the filter paper (3) needing setting depending on cultivation or do not arrange on the sponge (4) of loose structure; The sponge ring (6) needing setting depending on cultivation or do not arrange under being placed in the sponge (4) of loose structure; Be placed in the air filter (5) on top cover (1); Wherein: the sponge (4) of loose structure is for which is provided with the sponge of the loose structure of a pit.
The utility model adopts commercial part and material to be prepared from according to a conventional method.
Of the present utility modelly effectively to be:
1, owing to being provided with the sponge of loose structure in transparent vessel, can culture be supported and not be immersed in liquid nutrient medium, improving the supply of oxygen, effectively avoiding the problem of vitrifying, lopsided seedling.
2, Bian liquid nutrient medium, saves agar resource, improves that medium is inner nutraceutically to be absorbed, and reduces costs and has positive effect with saving resource aspect.
3, adopt liquid culture, culture is more quick to the absorption of nutrient, and growth rate is faster, and culture efficiency is high, shortens incubation time.
4, by regulating the thickness of sponge, can deep-layer liquid cultivation be carried out, and extend the time of subculture, and then save incubation time.
Accompanying drawing illustrates:
Fig. 1 is main TV structure schematic diagram when arranging 1 sponge ring (6) under the utility model.
Fig. 2 is the vertical view of the utility model top cover.
Fig. 3 is the schematic diagram of the utility model sponge (4).
Fig. 4 is the utility model sponge ring (6) schematic diagram.
Embodiment:
Below in conjunction with drawings and Examples, the utility model is described further.
The utility model comprises the container 2 of hyalosome structure, is provided with the top cover 1 of filtering microorganism parts, is placed in the sponge 4 of the loose structure of the bottom of container 2, the filter paper 3 needing setting depending on cultivation or do not arrange on the sponge 4 of loose structure; The sponge being placed in loose structure needs arrange or do not arrange sponge ring 6 depending on cultivating for 4 times; Be placed in the air filter 5 on top cover 1; Wherein: the sponge 4 of loose structure is for which is provided with the sponge of the loose structure of a pit.Container 2 and top cover 1 are for being threaded.The quantity of sponge ring 6 looks to cultivate to be needed to determine.
Liquid nutrient medium routine used loads container 2, puts into the sponge 4 of loose structure, sponge is placed filter paper 3, top cover 1, cool stand-by after conventional high-pressure sterilizing.
Embodiment 1: the deep-layer liquid of carnation cultivates (contrasting with solid culture medium)
Adventitious bud inducing:
Be placed in the sponge 4 for loose structure of container 2, sponge 4 placed filter paper 3, do not establish sponge ring 6.
Under aseptic condition, in the middle part of aseptic nipper extruding sponge, squeeze away air, filter paper is connected with liquid nutrient medium by sponge, by aseptic carnation blade inoculation on filter paper 3, put into culturing room and cultivate.10 days subcultures once, until there is a large amount of adventitious bud formation.
Indefinite bud strong sprout:
Be placed in the sponge 4 of the loose structure of container 2 for which is provided with the sponge of the loose structure of a pit.Sponge 4 divides into 2 or 3 sponge rings 6.
Aseptically, with the pit in the middle part of aseptic nipper extruding sponge, squeeze away air, by the pit of the carnation blade inoculation of existing differentiation adventitious buds to sponge, put into culturing room and cultivate.10 days subcultures once, until the height of indefinite bud is 4cm, have 8 leaves.
Embodiment 2: taking root of carnation indefinite bud
As shown in Figure 1, be placed in the sponge 4 for loose structure of container 2 for which is provided with the sponge 4 of the loose structure of a pit, sponge 4 is placed filter paper 3, sponge 4 divides into 1 sponge ring 6.
Under aseptic condition, in the middle part of aseptic nipper extruding sponge, squeeze away air, filter paper 3 is connected with liquid nutrient medium by sponge, the aseptic carnation indefinite bud with 8 leaves is seeded on filter paper 3, put into culturing room and cultivate.After taking root, hardening, transplanting, form the root system being rich in fibrous root in a large number in 10 days.
Contrast, in Table by the deep-layer liquid cultivation results of embodiment 1 and embodiment 2 with by the result that solid culture medium is cultivated:
Comparative result is known: adopt the utility model and the conventional liquid nutrient medium used to cultivate plant, effectively can not only avoid the problem of vitrifying, lopsided seedling, can also improve culture efficiency, shorten incubation time.
Claims (2)
1. a Plants deep-layer liquid tissue culture vessel, comprise the container (2) of hyalosome structure, be provided with the top cover (1) of filtering microorganism parts, it is characterized in that: it also comprises the sponge (4) of the loose structure of the bottom being placed in container (2), be placed in the filter paper (3) needing setting depending on cultivation or do not arrange on the sponge (4) of loose structure, the sponge ring (6) needing setting depending on cultivation or do not arrange under being placed in the sponge (4) of loose structure, is placed in the air filter (5) on top cover (1).
2., by a Plants deep-layer liquid tissue culture vessel according to claim 1, it is characterized in that, the sponge (4) of described loose structure is for which is provided with the sponge of the loose structure of a pit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520170279.8U CN204560518U (en) | 2014-12-09 | 2015-03-25 | One Plants deep-layer liquid tissue culture vessel |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420774627 | 2014-12-09 | ||
| CN2014207746278 | 2014-12-09 | ||
| CN201520170279.8U CN204560518U (en) | 2014-12-09 | 2015-03-25 | One Plants deep-layer liquid tissue culture vessel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204560518U true CN204560518U (en) | 2015-08-19 |
Family
ID=53853231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520170279.8U Expired - Fee Related CN204560518U (en) | 2014-12-09 | 2015-03-25 | One Plants deep-layer liquid tissue culture vessel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204560518U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173228A (en) * | 2017-06-27 | 2017-09-19 | 贵州村华秋实现代生态农业有限公司 | A kind of red kiwifruit method for culturing seedlings |
-
2015
- 2015-03-25 CN CN201520170279.8U patent/CN204560518U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173228A (en) * | 2017-06-27 | 2017-09-19 | 贵州村华秋实现代生态农业有限公司 | A kind of red kiwifruit method for culturing seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102119655B (en) | Natural light rapid breeding method for dendrobium officinale | |
| CN203048953U (en) | Dismountable plant tissue fluid culture device | |
| CN107155896B (en) | A method of promote African Chrysanthemum tissue culture to transplant seedling rooting | |
| CN103382443A (en) | Novel method for culturing microalgae | |
| CN202958378U (en) | Circulation type soilless culture device | |
| CN103548660B (en) | Plant seedling pot allowing automatic liquid absorption | |
| CN103146525B (en) | Production method of soft type composite multi-micro-function yeast | |
| CN204560518U (en) | One Plants deep-layer liquid tissue culture vessel | |
| CN206596365U (en) | A kind of incubator for cultivating seeds | |
| CN204968759U (en) | Iris stereoscopic cultivate frame | |
| CN204968690U (en) | Modular cave dish | |
| CN204185472U (en) | A kind of spawn fermentation tank of homogeneous heating | |
| CN103662513A (en) | Garbage can capable of being used for planting flowers and grass | |
| CN204409165U (en) | Cultivation apparatus without soil | |
| CN203761997U (en) | Oxygen aeration device for plant soilless culture | |
| CN202385593U (en) | Root-division cultivation device | |
| CN203072563U (en) | Hyacinth cultivation container | |
| CN104255453B (en) | A kind of store method of stevia plantlet in vitro | |
| CN204014679U (en) | A kind of peanut nutrient solution culture apparatus | |
| CN206227148U (en) | A kind of special cultivation alms bowl of oil tea nursery stock | |
| CN104067937B (en) | A kind of method of tidal type bio-reactor and cultivation lily seed ball thereof | |
| CN104170741B (en) | A kind of Storaged media of chrysanthemum tissue cultured seedling | |
| CN105993902B (en) | A kind of planting unit of ornamental waterweed | |
| CN103461090B (en) | Filament seedling and mariculture method of grateloupia filicina | |
| CN105145357B (en) | Potato virus-free plantlet liquid culturing apparatus and Liquid Culture quick-breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150819 Termination date: 20160325 |