CN105145357B - Potato virus-free plantlet liquid culturing apparatus and Liquid Culture quick-breeding method - Google Patents
Potato virus-free plantlet liquid culturing apparatus and Liquid Culture quick-breeding method Download PDFInfo
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- CN105145357B CN105145357B CN201510571967.XA CN201510571967A CN105145357B CN 105145357 B CN105145357 B CN 105145357B CN 201510571967 A CN201510571967 A CN 201510571967A CN 105145357 B CN105145357 B CN 105145357B
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Abstract
The present invention relates to a kind of potato virus-free plantlet liquid culturing apparatus.The potato virus-free plantlet liquid culturing apparatus includes nutrition pond, arranged on the seedbed of the nutrition pond and blake bottle, the nutrition pond includes pond body, the pond body includes inlet, leakage fluid dram and control valve, nutrient solution can be flowed by inlet and leakage fluid dram, the seedbed includes bed body and elevation and subsidence regulating device, the bed body includes field planting panel and multiple field planting holes through field planting panel, and the blake bottle is arranged on the field planting hole.The invention further relates to a kind of Liquid Culture quick-breeding method using the potato virus-free plantlet liquid culturing apparatus culture potato virus-free plantlet, standing liquid culture and intermittent Liquid Culture are combined by the technology, so that the culture of test tube seedling has more the advantage of high efficiency and low cost, in addition, the index such as Potato Plantlets in vitro cycle time, stem is thick, plant height, leaf area, dry weight is obviously improved.
Description
Technical field
The present invention relates to the reproduction technique of potato, belong to agricultural plantation technology field, and in particular to potato virus-free plantlet
Liquid culturing apparatus and its Liquid Culture quick-breeding method.
Background technology
At present, the culture of potato virus-free plantlet, mainly includes the form of solid, semisolid or fluid nutrient medium, common
There are labour intensive, the shortcoming such as of high cost, the degree of automation is low for tissue-cultured seedling solid and Semi-solid cell culture.From the 1980s
Mid-term starts, and western countries begin attempt to use the form of fluid nutrient medium to carry out Liquid Culture to potato virus-free plantlet.Mesh
Before, potato Liquid Culture generally using standing type fluid nutrient medium carry out, its have adapt to large-scale production, reduce cost and
Reduce the advantages such as labor cost.But since fluid nutrient medium is standing type, cause in Liquid Culture with the growth of time,
Fluid nutrient medium active ingredient is difficult to the timely and effective fresh nutrient of supplement after having been absorbed by seedling, often result in lopsided seedling and test tube seedling hair
The problems such as bad is educated, seriously affects the culture of test tube seedling, adds cultivation cost.
In addition, current potato test tube seedling Liquid Culture is generally by the way of the independent standing type culture of vial, and
Operation to culture medium in vial can there are labor and time it is with high costs the problem of, cause efficiency low and of high cost.Therefore,
The culture apparatus of a set of suitable potato Liquid Culture is designed, establishes new cultural method system, for reducing human cost consumption
Take, realize automation culture, reduce large-scale commercial operation cost, have significant Social benefit and economic benefit.
The content of the invention
In order to easily cause test tube seedling depauperation and manually training when solving above-mentioned potato test tube seedling standing type Liquid Culture
Technical problem of high cost is supported, the present invention provides a kind of high good seedling rate of test tube seedling, the degree of automation height and manually toxigenic capacity is low
Potato virus-free plantlet liquid culturing apparatus and its Liquid Culture quick-breeding method.
The present invention provides a kind of potato virus-free plantlet liquid culturing apparatus, including nutrition pond, seedbed and blake bottle；The battalion
Supporting pond includes pond body and protective film, the pond body one end open and has receiving space, and the protective film is covered in the pond body
Openend, the pond body include inlet, leakage fluid dram and control valve, and the inlet and the leakage fluid dram are respectively arranged on the pond
Body both ends are simultaneously connected with its inside, and the control valve is respectively arranged on the inlet and the leakage fluid dram；The seedbed is arranged on institute
In nutrition pond, the seedbed includes bed body and adjusts the lift adjustment that the bed body rises or falls in the nutrition pond
Device, the bed body include the field planting hole of field planting panel and multiple uniform intervals spread configurations, and the field planting hole is through described
It is colonized panel；The blake bottle is arranged on the seedbed by the field planting hole, and the blake bottle includes multiple stomatas and filter paper,
The stomata runs through the side wall and bottom of bottle arranged on the blake bottle, and the filter paper is arranged in the blake bottle and is layed in bottle
Bottom.
In a kind of preferred embodiment of potato virus-free plantlet liquid culturing apparatus provided by the invention, the protective film is
PVC film.
In a kind of preferred embodiment of potato virus-free plantlet liquid culturing apparatus provided by the invention, the lift adjustment
Device is slide rail type elevation and subsidence regulating device.
In a kind of preferred embodiment of potato virus-free plantlet liquid culturing apparatus provided by the invention, the blake bottle number
Measure to be multiple, respectively the detachable field planting hole arranged on the seedbed.
The present invention provides a kind of potato virus-free plantlet Liquid Culture quick-breeding method, include the following steps：
Step 1: provide potato virus-free plantlet liquid culturing apparatus, fluid nutrient medium and virus-free potato tube plant；
The potato virus-free plantlet liquid culturing apparatus includes nutrition pond, seedbed and blake bottle；
The nutrition pond includes pond body and protective film, the pond body one end open and tool receiving space, the protective film cover
Arranged on the openend of the pond body, the pond body includes inlet, leakage fluid dram and control valve, the inlet and the leakage fluid dram
It is respectively arranged on the pond body both ends and is connected with its inside, the control valve is respectively arranged on the inlet and the leakage fluid dram；
The seedbed includes bed body and adjusts the lift adjustment that the bed body rises or falls in the nutrition pond
Device, the bed body include the field planting hole of field planting panel and multiple uniform intervals spread configurations, and the definite value hole is through described
It is colonized panel；
The blake bottle includes multiple stomatas and filter paper, and the stomata runs through the side wall and bottle arranged on the blake bottle
Bottom, the filter paper are arranged in the blake bottle and are layed in bottom of bottle；
The preparation of the fluid nutrient medium：Using MS culture mediums as basic culture medium, and addition is as follows in 1L MS culture mediums
Component, and make the final concentration of each component as follows：NAA0.05-0.1mg/L, 6-BA1.0-1.5mg/L, sucrose 30-40g/l, and adjust
PH value is saved to 5.6-5.8；
Step 2: it will be inoculated with after virus-free potato tube plant shearing and sterilization processing；
Specifically, the virus-free potato tube plant is cut into the bud section that length is 1-3cm by bud point, is placed in filter paper
Suck dry moisture, uses HgCl2, NaClO and 70-75% alcohol carry out disinfection sterilization treatment, then with aseptic water washing 4-5 times, and
With filter paper suck dry moisture, finally it is seeded on the filter paper in the blake bottle；
Step 3: the prepared fluid nutrient medium of step 1 is delivered in the pond body by the inlet,
And maintain certain altitude；
Step 4: the blake bottle to be arranged on to the field planting hole in the seedbed, and institute is adjusted by the elevation and subsidence regulating device
State the vertical height in seedbed so that the fluid nutrient medium in the pond body did not had the filter paper in the blake bottle just；
Step 5: being started first using standing liquid culture 2-3d to tooth section after taking root, then trained again using liquid batch
Support 5-10d.
In a kind of preferred embodiment of potato virus-free plantlet Liquid Culture quick-breeding method provided by the invention, in step 1
In, the potato virus-free plantlet liquid culturing apparatus and the fluid nutrient medium carry out vacuum sterilization 15-20min before use,
Vacuum is 0.1-0.15Mpa, and the temperature of sterilizing is 121 DEG C -128 DEG C.
In a kind of preferred embodiment of potato virus-free plantlet Liquid Culture quick-breeding method provided by the invention, in step 5
In, the standing liquid culture is the height by adjusting the seedbed so that the fluid nutrient medium in the pond body did not had institute
State the bud section 0.2-0.5mm on filter paper, and at 25 ± 2 DEG C, light application time 12-16h/d, intensity of illumination 2000-3000LUX conditions
Under in the blake bottle bud section carry out illumination cultivation；
The liquid batch culture is by the intermittent height for adjusting the seedbed of the elevation and subsidence regulating device so that institute
State the fluid nutrient medium in pond body and do not have bud section 0.2-0.5mm on the filter paper, or in excessively not described culture
The state of filter paper in bottle, intermittent adjusting frequency are 3-5 times/day, and the time for there be not filter paper every time maintains 5-10 points
Clock, carries out illumination cultivation under the conditions of natural light.
In a kind of preferred embodiment of potato virus-free plantlet Liquid Culture quick-breeding method provided by the invention, in step 5
In, further include and ingredient analysis periodically is carried out to the fluid nutrient medium.
Compared to the prior art, potato virus-free plantlet liquid culturing apparatus provided by the invention and its Liquid Culture numerous side soon
Method has the advantages that：
First, by the design of potato virus-free plantlet liquid culturing apparatus, the step of potato virus-free plantlet Liquid Culture is simplified
Suddenly, reduce human cost consuming, reduce operation cost, realize significant Social benefit and economic benefit；Pass through Ma Ling
The design of the inlet and leakage fluid dram of potato detoxic seedling liquid culturing apparatus so that the fluid nutrient medium cost-effectively energy in nutrition pond
Access and effectively and timely supplement, avoid the malnutrition of test tube seedling and the situation of lopsided seedling occur, improve test tube seedling into
Motility rate and good seedling rate；
2nd, by the quick-breeding method based on potato virus-free plantlet liquid culturing apparatus of targeted design, using standing liquid
Body culture and liquid batch culture, coordinate the fluid nutrient medium and condition of culture of targeted design so that cultivation cycle shortens
22%-30%, and turn out the test tube seedling come have and grow fine (such as stem is thick, plant height, leaf area, dry weight index have it is aobvious
Write lifting), viral infection rate is low and the advantage further cultivated is needed after being easy to；
3rd, intermittent submergence is carried out to test tube seedling by using potato virus-free plantlet liquid culturing apparatus to cultivate, liquid training
Support base by it is original can subculture increase to twice three times, greatly reduce cost, at the same liquid batch cultivation stage be from
Cultivated under right illumination condition, it is possible to reduce power consumption.
Brief description of the drawings
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing, wherein：
Fig. 1 is the structure diagram of potato virus-free plantlet liquid culturing apparatus provided by the invention；
Fig. 2 is the seedbed frame schematic diagram of potato virus-free plantlet liquid culturing apparatus shown in Fig. 1；
Fig. 3 is the blake bottle structure diagram of potato virus-free plantlet liquid culturing apparatus shown in Fig. 1；
Fig. 4 is the step flow chart of potato virus-free plantlet Liquid Culture quick-breeding method provided by the invention.
Embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only the part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained all other without making creative work
Embodiment, belongs to the scope of protection of the invention.
This experiment is using the detoxification test tube plantlet of No. 3 potatos of river in Zhejiang Province potato as material to be tested, and by Ningbo of Zhejiang, Academy of Agricultural Sciences is studied
Institute development centre provides.
This experiment carries out in Ningbo City, Zhejiang Province academy of agricultural sciences Tissue Culture Center.
Referring to Fig. 1, it is the structure diagram of potato virus-free plantlet liquid culturing apparatus provided by the invention.The Ma Ling
Potato detoxic seedling liquid culturing apparatus 1 includes nutrition pond 11, feed system 13, seedbed 15 and blake bottle 17, the feed system
13 are connected with the nutrition pond 11, and for supplying fluid nutrient medium to the nutrition pond 11, the seedbed 15 is in horizontal direction
In institute's nutrition pond 11, the blake bottle 17 is detachable to be arranged on the seedbed 15.
The nutrition pond 11 is used to hold fluid nutrient medium, it includes pond body 111 and covers in the protection of the pond body 111
Film 113.
111 one end open of pond body and tool receiving space, the pond body 111 include inlet 1111, leakage fluid dram 1113
With control valve 1115, the inlet 1111 and the leakage fluid dram 1113 be respectively arranged on the both ends of the pond body 111 and with it
Portion connects, and the control valve 1115 is respectively arranged on the inlet 1111 and the leakage fluid dram 1113, and the inlet 1111 is used
In fluid nutrient medium input in the nutrition pond 11 and fluid nutrient medium is replaced, the leakage fluid dram 1113 is used for the nutrition pond 11
Interior fluid nutrient medium discharge and replacement fluid nutrient medium, the quantity of the control valve 1115 is two, for the nutrition pond 11
Interior fluid nutrient medium total capacity control.
The protective film 113 is covered in the openend of the pond body 111, for the inside of nutrition pond 11 and external environment
Isolation, the protective film 113 is PVC film.
On the one hand the feed system 13 can be that the nutrition pond 11 provides fluid nutrient medium, on the other hand recyclable liquid
Culture medium is simultaneously used for other crops, reduces environmental pollution.The feed system 13 include circulating tank 131, circulation inlet tube 133,
Circulating pump 135 and circulation outlet tube 137.
The circulating tank 131 is used to store fluid nutrient medium, and 133 one end of the circulation inlet tube connects the circulating tank
131, the other end connects the inlet 1111, and the circulating pump 135, institute are provided with the pipeline of the circulation inlet tube 133
State and the control valve 1115 is installed between circulating pump 135 and the inlet 1111；The connection of 137 one end of the circulation outlet tube
The circulating tank 131, the other end connect the liquid outlet 1113, the control valve are also provided with the circulation outlet tube 137
1115。
Please in conjunction with refering to Fig. 2, Fig. 2 is the seedbed frame schematic diagram of potato virus-free plantlet liquid culturing apparatus shown in Fig. 1.
The seedbed 15 includes bed body 151 and adjusts the rising-falling tone that the bed body 151 rises or falls in the nutrition pond 11
Regulating device 153, the bed body 151 include field planting panel 1511 and field planting hole 1513, the field planting hole 1513 be it is multiple, uniformly
It is spaced and is set through the field planting panel 1511, the blake bottle 17 is removably solid by the field planting hole 1513
Arranged on the field planting panel 1511.
The elevation and subsidence regulating device 153 is slide rail type elevation and subsidence regulating device.
Referring to Fig. 3, Fig. 3 is the blake bottle structure diagram of potato virus-free plantlet liquid culturing apparatus shown in Fig. 1.It is described
Blake bottle 17 distinguish it is detachable be arranged on the field planting hole 1513 in, the blake bottle 17 includes multiple stomatas 171 and filter paper
173, the stomata 171 runs through the side wall and bottom of bottle arranged on the blake bottle 17, and the filter paper 173 is arranged on the blake bottle 17
It is interior and be layed in bottom of bottle.
Referring to Fig. 4, it is the step flow chart of potato virus-free plantlet Liquid Culture quick-breeding method provided by the invention.It is described
Potato virus-free plantlet Liquid Culture quick-breeding method, includes the following steps：
Step S1, potato virus-free plantlet liquid culturing apparatus, fluid nutrient medium and virus-free potato tube plant are provided；
The potato virus-free plantlet liquid culturing apparatus includes nutrition pond, seedbed and blake bottle；
The nutrition pond includes pond body and protective film, the pond body one end open and tool receiving space, the pond body bag
Include inlet, leakage fluid dram and control valve, the inlet and the leakage fluid dram be respectively arranged on the pond body both ends and with inside it
Connection, the control valve are respectively arranged on the inlet and the leakage fluid dram, and the protective film is covered in the opening of the pond body
End；
The seedbed includes bed body and adjusts the rising-falling tone that the bed body rises or falls in the nutrition pond
Regulating device, the bed body include the field planting hole of field planting panel and multiple uniform intervals spread configurations, and the field planting hole runs through institute
State field planting panel；
The blake bottle includes multiple stomatas and filter paper, and the stomata, which runs through, is arranged on the blake bottle side wall and bottom of bottle,
The filter paper is arranged in the blake bottle and is layed in bottom of bottle；
The preparation of the fluid nutrient medium：MS culture mediums+NAA0.05-0.1mg/L, 6-BA1.0-1.5mg/L, sucrose 30-
40g/l, and pH value is adjusted to 5.6-5.8.
Step S2, vacuum sterilization is carried out to the potato virus-free plantlet liquid culturing apparatus and fluid nutrient medium；
Vacuum sterilization condition：Time is 15-20min, and vacuum 0.1-0.15Mpa, the temperature of sterilizing is 121 DEG C -128
℃。
Step S3, by the virus-free potato tube plant shear and sterilization processing after be inoculated with；
Specifically, the virus-free potato tube plant is cut into the bud section that length is 1-3cm, preferred length by bud point
For the bud section of 1.5-2.6cm, filter paper suck dry moisture is placed in, is carried out disinfection with the alcohol of HgCl2, NaClO and 70-75% at sterilizing
Reason, then with aseptic water washing 4-5 times, and with filter paper suck dry moisture, be finally seeded on the filter paper in the blake bottle；
Step S4, the prepared fluid nutrient medium is inputted to the pond body, and maintains certain altitude, fallen at the same time
Enter suitable bacteriostatic agent, further reduce the risk of catching an illness of the virus-free potato tube plant；
Step S5, the blake bottle is put into the seedbed, and the seedbed is adjusted by the elevation and subsidence regulating device
Vertical height so that the fluid nutrient medium in the pond body did not had the filter paper in the blake bottle just；
Step S6, started first using standing liquid culture 2-3d to tooth section after taking root, then using liquid batch culture 5-
10d；
Specific condition of culture is as follows：
Standing liquid culture：By the height for adjusting seedbed so that the fluid nutrient medium in the pond body did not had the filter
Bud section 0.2-0.5mm on paper, is preferably 0.5mm, and at 25 ± 2 DEG C, light application time 12-16h/d, intensity of illumination 2000-
Illumination cultivation is carried out to the bud section in the blake bottle under the conditions of 3000LUX；
Liquid batch culture：Pass through the intermittent height for adjusting seedbed of the elevation and subsidence regulating device so that in the pond body
Fluid nutrient medium do not had bud section 0.2-0.5mm, preferably 0.3mm on the filter paper, or in excessively not described training
The state of the filter paper in bottle is supported, intermittent adjusting frequency is 3-5 times/day, preferably 5 times/day, does not have time of filter paper every time
5-10 minutes, preferably 8 minutes are maintained, illumination cultivation is carried out under the conditions of natural light, final to obtain potato test tube seedling, it is high
Degree reaches 9-12cm, and root system is sturdy, and root long averagely reaches 8-10cm, and the quantity of root reaches 10-15 roots.At the same time including periodically to institute
State fluid nutrient medium composition to be detected, the Liquid Culture that the nutrition by detecting fluid nutrient medium can more renew in time
Base.Illumination is carried out under the conditions of natural light to support, and can reduce power consumption cost.
After one cultivation cycle, the fluid nutrient medium can be collected into corresponding container by leakage fluid dram, carry out ingredient analysis
Afterwards, then again fluid nutrient medium described in preparation steps one, fluid nutrient medium can be circulated and used three times, greatly reduce and be trained
This.The fertilising that the fluid nutrient medium after use three times can be used for other crops is circulated, reduces environmental pollution.
Potato virus-free plantlet liquid culturing apparatus and Liquid Culture quick-breeding method provided by the invention have below beneficial to effect
Fruit：
First, by the design of potato virus-free plantlet liquid culturing apparatus, the step of potato virus-free plantlet Liquid Culture is simplified
Suddenly, reduce human cost consuming, reduce operation cost, realize significant Social benefit and economic benefit；Pass through Ma Ling
The design of the inlet and leakage fluid dram of potato detoxic seedling liquid culturing apparatus so that the fluid nutrient medium cost-effectively energy in nutrition pond
Access and effectively and timely supplement, avoid the malnutrition of test tube seedling and the situation of lopsided seedling occur, improve test tube seedling into
Motility rate and good seedling rate；
2nd, by the quick-breeding method based on potato virus-free plantlet liquid culturing apparatus of targeted design, using standing liquid
Body culture and liquid batch culture, coordinate the fluid nutrient medium and condition of culture of targeted design so that cultivation cycle shortens
22%-30%, and turn out the test tube seedling come have and grow fine (such as stem is thick, plant height, leaf area, dry weight index have it is aobvious
Write lifting), viral infection rate is low and the advantage further cultivated is needed after being easy to；
3rd, intermittent submergence is carried out to test tube seedling by using potato virus-free plantlet liquid culturing apparatus to cultivate, liquid training
Support base by it is original can subculture increase to twice three times, greatly reduce cost, at the same liquid batch cultivation stage be from
Cultivated under right illumination condition, it is possible to reduce power consumption.
The foregoing is merely the embodiment of the present invention, is not intended to limit the scope of the invention, every to utilize this hair
The equivalent structure or equivalent flow shift that bright specification and accompanying drawing content are made, is directly or indirectly used in other relevant skills
Art field, is included within the scope of the present invention.
Claims (3)
1. a kind of potato virus-free plantlet Liquid Culture quick-breeding method, it is characterised in that include the following steps：
Step 1: provide potato virus-free plantlet liquid culturing apparatus, fluid nutrient medium and virus-free potato tube plant；
The potato virus-free plantlet liquid culturing apparatus includes nutrition pond, seedbed and blake bottle；
The nutrition pond includes pond body and protective film, the pond body one end open and tool receiving space, the protective film cover in
The openend of the pond body, the pond body include inlet, leakage fluid dram and control valve, the inlet and leakage fluid dram difference
Connected arranged on the pond body both ends and with its inside, the control valve is respectively arranged on the inlet and the leakage fluid dram；
The seedbed includes bed body and adjusts the elevation and subsidence regulating device that the bed body rises or falls in the nutrition pond,
The bed body includes the field planting hole of field planting panel and multiple uniform intervals spread configurations, and the field planting hole runs through the field planting face
Plate；
The blake bottle includes multiple stomatas and filter paper, and the stomata runs through the side wall and bottom of bottle arranged on the blake bottle, institute
Filter paper is stated in the blake bottle and is layed in bottom of bottle；
The preparation of the fluid nutrient medium：Using MS culture mediums as basic culture medium, and addition such as the following group in 1L MS culture mediums
Point, and make the final concentration of each component as follows：NAA0.05-0.1mg/L, 6-BA 1.0-1.5mg/L, sucrose 30-40g/l, and adjust
PH value is saved to 5.6-5.8；
Step 2: it will be inoculated with after virus-free potato tube plant shearing and sterilization processing；
Specifically, the virus-free potato tube plant is cut into the bud section that length is 1-3cm by bud point, is placed in filter paper and blots water
Point, use HgCl2, NaClO and 70-75% alcohol carry out disinfection sterilization treatment, then with aseptic water washing 4-5 times, and use filter paper
Suck dry moisture, is finally seeded on the filter paper in the blake bottle；
Step 3: the prepared fluid nutrient medium of step 1 is delivered in the pond body by the inlet, and tie up
Hold certain altitude；
Step 4: the blake bottle to be arranged on to the field planting hole in the seedbed, and the seedling is adjusted by the elevation and subsidence regulating device
The vertical height of bed so that the fluid nutrient medium in the pond body did not had the filter paper in the blake bottle just；
Step 5: starting first using standing liquid culture 2-3d to tooth section after taking root, liquid batch culture 5- is then used again
10d；
Specifically, the standing liquid culture is the height by adjusting the seedbed so that the Liquid Culture in the pond body
Base did not had the bud section 0.2-0.5mm on the filter paper, and at 25 ± 2 DEG C, light application time 12-16h/d, intensity of illumination 2000-
Illumination cultivation is carried out to the bud section in the blake bottle under the conditions of 3000LUX；
The liquid batch culture is by the intermittent height for adjusting the seedbed of the elevation and subsidence regulating device so that the pond
Internal fluid nutrient medium did not had the bud section 0.2-0.5mm on the filter paper, or in excessively not described blake bottle
Filter paper state, intermittent adjusting frequency be 3-5 times/day, the time for there be not filter paper every time maintains 5-10 minutes,
Illumination cultivation is carried out under the conditions of natural light.
2. potato virus-free plantlet Liquid Culture quick-breeding method according to claim 1, it is characterised in that in step 1,
The potato virus-free plantlet liquid culturing apparatus and the fluid nutrient medium carry out vacuum sterilization 15-20min, vacuum before use
Spend for 0.1-0.15Mpa, the temperature of sterilizing is 121 DEG C -128 DEG C.
3. potato virus-free plantlet Liquid Culture quick-breeding method according to claim 1, it is characterised in that in step 5,
Further include and ingredient analysis periodically is carried out to the fluid nutrient medium.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510571967.XA CN105145357B (en) | 2015-09-10 | 2015-09-10 | Potato virus-free plantlet liquid culturing apparatus and Liquid Culture quick-breeding method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510571967.XA CN105145357B (en) | 2015-09-10 | 2015-09-10 | Potato virus-free plantlet liquid culturing apparatus and Liquid Culture quick-breeding method |
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| CN105145357B true CN105145357B (en) | 2018-04-13 |
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| CN115024219A (en) * | 2022-06-07 | 2022-09-09 | 江苏农林职业技术学院 | Liquid intermittent immersion cultivation method for potato tissue culture seedlings |
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| CN1074241C (en) * | 1997-01-22 | 2001-11-07 | 中国科学院遗传研究所 | Culture container and method for producing potato miniature potato etc. plant material using container thereof |
| CN201015341Y (en) * | 2007-05-11 | 2008-02-06 | 中国科学院沈阳应用生态研究所 | Intermittent immersion type plant incubators |
| CN201947752U (en) * | 2010-11-22 | 2011-08-31 | 北方民族大学 | Tissue culture bottle for liquid culturing of potato detoxification base seedlings |
| CN202617912U (en) * | 2012-04-25 | 2012-12-26 | 甘肃省农垦农业研究院 | Solid-liquid dual-purpose tissue culture bottle for plant |
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