CN201947752U - Tissue culture bottle for liquid culturing of potato detoxification base seedlings - Google Patents
Tissue culture bottle for liquid culturing of potato detoxification base seedlings Download PDFInfo
- Publication number
- CN201947752U CN201947752U CN 201020618094 CN201020618094U CN201947752U CN 201947752 U CN201947752 U CN 201947752U CN 201020618094 CN201020618094 CN 201020618094 CN 201020618094 U CN201020618094 U CN 201020618094U CN 201947752 U CN201947752 U CN 201947752U
- Authority
- CN
- China
- Prior art keywords
- bottle
- culturing
- tissue culture
- seedlings
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The utility model relates to a culture bottle for culturing potato base seedlings, in particular to a tissue culture bottle for liquid culturing of the potato detoxification base seedlings. The tissue culture bottle comprises a bottle body (3) and a bottle cover (2), wherein a ventilation filter film (1) is arranged on the bottle cover (2). The tissue culture bottle is characterized in that: a culturing plate (4) axially vertical to the bottle body (3) is arranged in the bottle body (3); and culturing holes (5) are formed on the culturing plate (4). The tissue culture bottle solves the problems that by liquid culturing, the seedlings cannot stand, the production cost is high, the growth of the seedlings is not as good as the growth of the seedlings cultured by the liquid culture medium; simultaneously, the production cost is reduced; when the annual capacity of each unit is 10,000,000 strains, about 24.3 yuan can be saved each year; and the culturing time of each generation is shorted for 5d. The tissue culture bottle is simple in structure, provides conditions for the liquid culturing, reduces the production cost and improves the production quality of the potato base seedlings.
Description
Technical field
The utility model relates to a kind of blake bottle that potato basis seedling is cultivated that is used for, especially potato detoxicating basis seedling liquid culture tissue culture bottle.
Background technology
The Nutrient Absorption that solid potato culture medium is supplied with explant is rapid not as liquid nutrient medium, and solid culture needs 20d to expand numerous 1 generation, liquid culture than and solid culture has shortened 5d, the average total dry weight of solid culture medium basis seedling growth is that to be that 4.40, root are long be 4.18mm for 121mg, the thick 1.07mm of stem, root bar number; The average total dry weight of liquid nutrient medium basis seedling growth is that 147mg, the thick 1.39mm of stem, root bar number are 10.40, the long 2.36mm of being of root, the short transplant survival that neatly helps young plant of root.
At present, the medium that is used for potato test tube basis seedling and propagating is generally the MS medium, and the medium fixture is an agar, and tissue culture bottle was made of bottle cap, ventilation filter membrane, bottle wall and the bottle end.Explant need be seeded in could be upright on the solid culture medium, need agar set thing 5g and prepare every liter of medium, thereby strengthened experimentation cost, the explant biological character of solid culture medium cultivation simultaneously is not as liquid nutrient medium,, root bar number thick etc. such as stem, thus the economic benefit of suitability for industrialized production potato influenced.
The utility model content
The purpose of this utility model provides a kind of potato detoxicating basis seedling liquid culture tissue culture bottle, can solve the defective that liquid nutrient medium can not be upright, and it is strong to reach the plant growing way by liquid culture, and Financial cost is low simultaneously.
For achieving the above object, the technical solution adopted in the utility model is:
A kind of potato detoxicating basis seedling liquid culture tissue culture bottle comprises bottle and bottle cap, is provided with the ventilation filter membrane on bottle cap, and its special feature is, is provided with in bottle and the axial vertical culture plate of bottle, has culture hole on this culture plate.
Wherein culture plate is stuck on the bottle inwall or culture plate is connected at an end with bottle by support, and culture plate and a bottle end spacing are 0.3-0.7cm.
Tissue culture bottle of the present utility model solved utilize liquid culture can not be uprightly, production cost is high and growing way not as the problem of liquid nutrient medium.Reduced production cost simultaneously, when the unit yearly productive capacity is 1,000 ten thousand strains, can save 24.3 ten thousand yuan every year, in incubation time per generation, shortened 5d.The utility model has structure comparatively simple, for liquid culture provides condition, has reduced production cost, has improved the quality of production of potato basis seedling.
Description of drawings
Accompanying drawing 1 is a structural representation of the present utility model.
Embodiment
The utility model is described in more detail below in conjunction with accompanying drawing: as shown in Figure 1, tissue culture bottle of the present utility model comprises bottle 3 and bottle cap 2, on bottle cap 2, be provided with ventilation filter membrane 1, thereby ventilation filter membrane 1 is circular mounting filtered air in the middle of bottle cap 2, ventilation filter membrane diameter is 1cm, is that the polyamide of 0.2 μ m is formed by the aperture.In bottle 3, be provided with and bottle 3 axial vertical culture plates 4, on this culture plate 4, have culture hole 5, the quantity of culture hole 5 and position and can decide in its sole discretion as required.The shape of culture plate 4 is identical with the shape of bottle 3 cross sections, promptly be circle, but culture plate 4 also can change thereupon when bottle 3 alterations of form obviously, culture plate 4 is stuck on bottle 3 inwalls or culture plate 4 is connected with the bottle end 6 by support, culture plate 4 general employing refractory polyethylene materials, and culture plate 4 is 0.3-0.7cm with the bottle ends 6 spacing.
Claims (2)
1. potato detoxicating basis seedling liquid culture tissue culture bottle, comprise bottle (3) and bottle cap (2), on bottle cap (2), be provided with ventilation filter membrane (1), it is characterized in that: in bottle (3), be provided with and the axial vertical culture plate (4) of bottle (3), on this culture plate (4), have culture hole (5).
2. potato detoxicating as claimed in claim 1 basis seedling liquid culture tissue culture bottle, it is characterized in that: wherein culture plate (4) is stuck on bottle (3) inwall or culture plate (4) is connected with a bottle end (6) by support, and culture plate (4) is 0.3-0.7cm with bottle end (a 6) spacing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020618094 CN201947752U (en) | 2010-11-22 | 2010-11-22 | Tissue culture bottle for liquid culturing of potato detoxification base seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020618094 CN201947752U (en) | 2010-11-22 | 2010-11-22 | Tissue culture bottle for liquid culturing of potato detoxification base seedlings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201947752U true CN201947752U (en) | 2011-08-31 |
Family
ID=44491884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020618094 Expired - Fee Related CN201947752U (en) | 2010-11-22 | 2010-11-22 | Tissue culture bottle for liquid culturing of potato detoxification base seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201947752U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123527A (en) * | 2015-09-17 | 2015-12-09 | 山东省农作物种质资源中心 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
| CN105145357A (en) * | 2015-09-10 | 2015-12-16 | 宁波市农业科学研究院 | Liquid culture device and liquid culture rapid propagating method for potato virus-free plantlets |
| CN110447430A (en) * | 2019-09-10 | 2019-11-15 | 普安县欣新生物科技有限责任公司 | A kind of bletilla culture bottle |
-
2010
- 2010-11-22 CN CN 201020618094 patent/CN201947752U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145357A (en) * | 2015-09-10 | 2015-12-16 | 宁波市农业科学研究院 | Liquid culture device and liquid culture rapid propagating method for potato virus-free plantlets |
| CN105123527A (en) * | 2015-09-17 | 2015-12-09 | 山东省农作物种质资源中心 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
| CN110447430A (en) * | 2019-09-10 | 2019-11-15 | 普安县欣新生物科技有限责任公司 | A kind of bletilla culture bottle |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203467354U (en) | Water-planting device for tissue culture seedlings | |
| CN106508685B (en) | The method and its culture bottle of " three stage an of step " production test tube potato | |
| CN201947752U (en) | Tissue culture bottle for liquid culturing of potato detoxification base seedlings | |
| CN2781738Y (en) | Sucking type cotton breeding seeder | |
| CN204443496U (en) | Virus-free seed potato water planting produces seedling growing container | |
| CN205954009U (en) | Algae aeration culture device | |
| CN201444795U (en) | Ground-to-air coupling land saving income increment all-weather multifunctional stereoscopic cultivating device | |
| CN201036286Y (en) | Liftable float grass cultivating rack | |
| CN206596365U (en) | A kind of incubator for cultivating seeds | |
| CN202958381U (en) | High-performance water culture crop seedling raising device | |
| CN202819197U (en) | Device for culturing of banana seedlings in water | |
| CN108029544B (en) | A culture apparatus for rice seedling | |
| CN103931478B (en) | A kind of floating on water surface planting unit | |
| CN203597158U (en) | Seedling cultivation hole tray | |
| CN204742122U (en) | Dual -purpose blake bottle of plant tissue culture solid -liquid | |
| CN204653247U (en) | Seed cultivation device | |
| CN208317762U (en) | A kind of test water training wheat crops device for raising seedlings | |
| CN104137776B (en) | Blueberry tissue cultured seedling liquid rooting method and special culturing container | |
| CN201690871U (en) | Plant water culture device | |
| CN203040262U (en) | Fruit and vegetable three-dimensional cultivation device | |
| CN202714008U (en) | Rapid seedling-growing container | |
| CN202364645U (en) | Matrix-soil relay cultivation device | |
| CN201426296Y (en) | High-flux culture box of test-tube-free plant tissue cultivated seedling | |
| CN203860182U (en) | On-water floating planting device | |
| CN201409354Y (en) | Low-cost high-throughout plant tissue culture bag |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110831 Termination date: 20141122 |
|
| EXPY | Termination of patent right or utility model |