CN106234229A - Method for temporarily planting taro tissue culture seedlings - Google Patents
Method for temporarily planting taro tissue culture seedlings Download PDFInfo
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- CN106234229A CN106234229A CN201610869732.3A CN201610869732A CN106234229A CN 106234229 A CN106234229 A CN 106234229A CN 201610869732 A CN201610869732 A CN 201610869732A CN 106234229 A CN106234229 A CN 106234229A
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- seedling
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- plant tissue
- fermentation
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- 238000000034 method Methods 0.000 title claims abstract description 47
- 244000205754 Colocasia esculenta Species 0.000 title abstract 2
- 235000006481 Colocasia esculenta Nutrition 0.000 title abstract 2
- 238000004161 plant tissue culture Methods 0.000 claims abstract description 86
- 241000196324 Embryophyta Species 0.000 claims abstract description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 53
- 239000003337 fertilizer Substances 0.000 claims abstract description 46
- 239000000758 substrate Substances 0.000 claims abstract description 37
- 238000004140 cleaning Methods 0.000 claims abstract description 23
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims description 86
- 230000004151 fermentation Effects 0.000 claims description 86
- 241000209094 Oryza Species 0.000 claims description 61
- 235000007164 Oryza sativa Nutrition 0.000 claims description 61
- 235000009566 rice Nutrition 0.000 claims description 61
- 241000221377 Auricularia Species 0.000 claims description 34
- 240000000599 Lentinula edodes Species 0.000 claims description 21
- 235000001715 Lentinula edodes Nutrition 0.000 claims description 21
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 13
- 229910052801 chlorine Inorganic materials 0.000 claims description 13
- 239000000460 chlorine Substances 0.000 claims description 13
- 240000000377 Tussilago farfara Species 0.000 claims description 11
- 235000004869 Tussilago farfara Nutrition 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 239000004744 fabric Substances 0.000 claims description 10
- 241000233866 Fungi Species 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 2
- 239000011499 joint compound Substances 0.000 abstract description 25
- 230000004083 survival effect Effects 0.000 abstract description 20
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 30
- 230000000052 comparative effect Effects 0.000 description 14
- 239000000203 mixture Substances 0.000 description 10
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- 239000005416 organic matter Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 230000035558 fertility Effects 0.000 description 5
- 239000007952 growth promoter Substances 0.000 description 5
- 238000009333 weeding Methods 0.000 description 5
- 244000037666 field crops Species 0.000 description 4
- 239000010408 film Substances 0.000 description 4
- 239000010409 thin film Substances 0.000 description 4
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
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- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
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- 238000012136 culture method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
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- 244000005700 microbiome Species 0.000 description 1
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- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of plant propagation, particularly provides a temporary planting method of a plant tissue culture seedling, and particularly relates to a temporary planting method of a taro tissue culture seedling. The method comprises the following steps: (1) hardening seedlings: hardening off the tissue culture seedlings of the plants; (2) cleaning: taking out the hardened plant tissue culture seedlings, sterilizing and then cleaning; (3) temporary planting: the cleaned plant tissue culture seedling is planted into a seedling field, and the substrate in the seedling field mainly comprises bacterial residue fermented fertilizer, mud and water. The method has the advantages of high planting speed, difficulty in generating weeds, low labor cost, short survival time and high survival rate.
Description
Technical field
The present invention relates to plant multiplication technique field, in particular to a kind of method of seedling of plant through temporary planting grouped cultivated, especially
Relate to a kind of method that Fructus Colocasiae Esculentae tissue cultured seedling is heeled in.
Background technology
The modes of reproduction of plant mainly has two kinds: 1, seminal propagation: seminal propagation is sexual propagation, and seed is by after planting
Root and then form plant；2, nourish and generate: nourish and generate also known as asexual propagation, mainly have separation, cuttage, press strip and transfer
Connecing, wherein the vegetative propagation of plant Seedling of isolated is called again tissue cultured seedling.Tissue cultured seedling be have according to plant cell totipotent
Theory, utilizes outside shade, and under aseptic and suitable artificial condition, the whole plant of cultivation, tissue cultured seedling reproduction speed is fast, cost
Low, high efficiency.
Existing Fructus Colocasiae Esculentae heeling in technology of tissue culture seedlings is to be transplanted in substrate by tissue cultured seedling in (nutrient cup or seedbed), and substrate uses
The Vermiculitum of 1:1:1, peat soil and perlite, or 4 parts of husky earth fertile soil, or be transplanted in sandy loam to heel in 1-2 and open blade and new
Root heels in nutrient cup again or seedbed is heeled in, and can be transplanted to land for growing field crops after 45-75 days.Prior art Culture media configures
Loaded down with trivial details, and plantation speed is slow, easily generates weeds, need more manually to carry out weeding, be unfavorable for that amount reproduction, management require height, system
About Fructus Colocasiae Esculentae tissue cultured seedling popularizing planting.
In view of this, the special proposition present invention.
Summary of the invention
A kind of method that it is an object of the invention to provide seedling of plant through temporary planting grouped cultivated, it is fast, difficult that the method has plantation speed
Generate weeds, cost of labor is low, become the advantage that live time is short and survival rate is high.
In order to realize the above-mentioned purpose of the present invention, spy by the following technical solutions:
A kind of method that the invention provides seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by plant tissue culture Seedling seedling exercising；
(2) clean: take out the plant tissue culture Seedling after seedling exercising, sterilization, then clean；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, the substrate in rice seedling bed mainly fertile by dreg fermentation,
Mud and water composition.
Plant tissue culture Seedling is to have totipotent theory according to plant cell, utilizes outside shade, aseptic and suitable people
Under the conditions of work, the whole plant of cultivation.Plant tissue culture i.e. plant Sterile Culture Methods Used, be have according to plant cell all-round
The theory of property, utilizes organ such as root, stem, leaf, stem apex, flower, fruit etc. that plant is in vitro) tissue is (such as cambium layer, epidermis, skin
Layer, marrow cell, endosperm etc.) or cell (such as megaspore, sporidiole, somatic cell etc.) and protoplast, aseptic and suitable
Synthetic medium and illumination, temperature etc. artificial under the conditions of, callus, adventitious bud, adventitious root can be induced, eventually formed
The subject of whole plant.Owing to plant tissue culture Seedling is to be formed under aseptic and suitable artificial condition, adapt to external environment
Ability, the survival rate that directly transplanting grows in big Tanaka's field planting is extremely low, in the present invention, plant tissue culture Seedling is being transplanted to land for growing field crops
Before heel in, improve plant tissue culture Seedling environment to external world adaptability, improve photosynthesis, promote tissue cultured seedling robust growth,
It is finally reached the successful purpose of transplanting.
First plant tissue culture Seedling carries out seedling exercising makes the adaptive capacity to environment of plant tissue culture Seedling be improved, and is prone to after transplanting
Survive.Then take out the plant tissue culture Seedling after seedling exercising, sterilized, clean, during seedling exercising, easily produce pollution, topmost
Having antibacterial and fungal contamination, the growth promoter of contaminated plant tissue culture Seedling can affect adversely, it is therefore desirable to carries out disinfection,
Kill noxious bacteria etc., promote the growth promoter of plant tissue culture Seedling, then clean.Then heel in, the plant after cleaning
Tissue cultured seedling is implanted in rice seedling bed, and the substrate in rice seedling bed in the present invention mainly, mud fertile by dreg fermentation and water form, and this substrate is to planting
Thing tissue cultured seedling growth promoter is useful, and substrate is in a liquid state, and plant tissue culture Seedling is directly put in rice seedling bed by workman, does not dig not
Blinding, heels in speed fast, is greatly saved cost of labor；Secondly the preparation of substrate is simple, dreg fermentation fertilizer and mud physics
Mixing, then passes to water, and dreg fermentation fertilizer includes abundant organic matter, it is possible to promote plant tissue culture Seedling healthy growth；Again
Secondary due to substrate be liquid, be not likely to produce weeds, decrease the cost of artificial weeding, the plant after using said method to heel in becomes
Motility rate is up to 98%, and saving of labor more than 70%.
Further, the bottom surface elder generation cloth good dreg fermentation fertilizer in described rice seedling bed, make the mud on dreg fermentation fertilizer and bottom surface, rice seedling bed
It is mutually mixed, then passes to water, soak 7-30 days.The configuration process of substrate is simple, saving of work and time.
Preferably, described dreg fermentation fertilizer is prepared by dreg and leaven, and fermentation time is 20-40 days, during above-mentioned fermentation
Between typical case but indefiniteness for 20,22,24,26,28,30,32,34,36,38 or 40 days.
Preferably, the height of bottom surface, water surface distance rice seedling bed is 5-10cm, in the present invention, above-mentioned height typical case but indefiniteness
For 5,6,7,8,9 or 10cm..
Preferably, the height of bottom surface, water surface distance rice seedling bed is 8cm.
Further, the consumption that the fermentation of described dreg is fertile is 1.2-1.5kg/m2, i.e. the bacterium on bottom surface, every square metre of rice seedling bed
Slag fermentation fertilizer is 1.2-1.5kg, and the consumption fertile when dreg fermentation is 1.2-1.5kg/m2Time, the content of organic matter in substrate is
Good, will not be because fertility deficiency causes plant tissue culture Seedling dysplasia or produces the phenomenon of burning root because fertility is excessive；In the present invention, on
State consumption typical case but indefiniteness for 1.2,1.3,1.4 or 1.5kg/m2。
Further, described dreg is edible fungi residue.Edible fungi residue contains numerous available nutrient, as slightly
Albumen, crude fat, crude fibre, trace element etc., also have many bioactive substances, such as polysaccharide, organic acid, enzyme, phenol
The chemical substances such as material；The dreg fermentation fertilizer formed after edible fungi residue is fermented can improve substrate fertility, stimulate plant roots
It is the generation of nitrogen-fixing microorganism, is a kind of well bacterial manure.
Preferably, described dreg is Auricularia dreg or Lentinus Edodes dreg.Auricularia dreg or Lentinus Edodes dreg are in the effect of leaven
Under, the ratio changing into organic matter is high, and dreg utilization rate is high, secondly uses Auricularia dreg or the Auricularia of Lentinus Edodes dreg fermentation
The granule that dreg fermentation is fertile or the fermentation of Lentinus Edodes dreg is fertile is relatively thick, good permeability.
Further, the density of heeling in of described plant tissue culture Seedling is: spacing in the rows 10-20cm, line-spacing 10-20cm.When plant group
When the spacing in the rows of seedlings cultivating and line-spacing are 10-20cm, heeling in density optimal, plant tissue culture Seedling growth promoter is good, does not haves and sends out
Educate the bad or phenomenon of substrate waste.
Preferably, the density of heeling in of described plant tissue culture Seedling is: spacing in the rows 15cm, line-spacing 15cm.
Preferably, described in the temperature heeled in be 15-35 DEG C, said temperature typical case but indefiniteness location 15,16,17,18,
19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34 or 35 DEG C.
Preferably, described in the humidity heeled in be 90%-98%, above-mentioned humidity typical case but indefiniteness for 90%, 91%,
92%, 93%, 94%, 95%, 96%, 97% or 98%.
Further, described plant tissue culture Seedling is Fructus Colocasiae Esculentae tissue cultured seedling or Horse hoof tissue cultured seedling.
Further, described seedling exercising outdoor scattered light or have 70% shading rate seedling growth greenhouse in carry out, seedling exercising temperature is
15-35 DEG C, the seedling exercising time is 1-2 days；
Described sterilization is that the strong chlorine oil aqueous solution using mass concentration to be 0.1%-0.3% carries out disinfection.
Further, described method is further comprising the steps of:
(4) railway carriage or compartment is played；
(5) described plant tissue culture Seedling is hidden with film and/or sunshade net；
(6) drainage trenching outside railway carriage or compartment.
Play railway carriage or compartment to be capable of heeling on a large scale；Plant tissue culture Seedling is hidden it can be avoided that plant tissue culture Seedling quilt with film or sunshade net
High light is tanned severely, and improves survival rate further；Outside railway carriage or compartment, water unnecessary in railway carriage or compartment can timely and effective be discharged by drainage trenching, it is to avoid plant
Tissue cultured seedling is flooded.
Further, a width of 0.8-1.2m in railway carriage or compartment in described railway carriage or compartment, the degree of depth in described gutter is 20-35cm, described draining
The width of ditch is 40-45cm, and the shading rate of described sunshade net is 70%-75%.
Preferably, the degree of depth in described gutter is 25cm.
Further, the method for described seedling of plant through temporary planting grouped cultivated comprises the following steps:
(1) seedling exercising: by Fructus Colocasiae Esculentae tissue cultured seedling or Horse hoof tissue cultured seedling outdoor scattered light or have 70% shading rate seedling growth greenhouse in
Carrying out seedling exercising, seedling exercising temperature is 15-35 DEG C, seedling exercising 1-2 days；
(2) cleaning: take out the plant tissue culture Seedling after seedling exercising, the strong chlorine oil aqueous solution using mass concentration to be 0.2% is carried out
Sterilization, then cleans with clear water；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, the substrate in rice seedling bed mainly fertile by dreg fermentation,
Mud and water composition, the good consumption of bottom surface elder generation cloth in rice seedling bed is 1.2-1.5kg/m2Dreg fermentation fertilizer, make dreg fermentation fertile and rice seedling bed
Mud on bottom surface is mutually mixed, and then passes to water, soaks 7-30 days；The height of bottom surface, water surface distance rice seedling bed is 8cm, plant tissue culture
The density of heeling in of Seedling is spacing in the rows 15cm, line-spacing 15cm, and the temperature heeled in is 25 DEG C, and the humidity heeled in is 95%；Described dreg is sent out
Ferment fertilizer is prepared by Auricularia dreg or Lentinus Edodes dreg and leaven, and fermentation time is 20-40 days；
(4) railway carriage or compartment is played: play a width of 1m in railway carriage or compartment in railway carriage or compartment；
(5) described plant tissue culture Seedling is hidden with film and/or sunshade net: the shading rate of sunshade net is 70%-75%；
(6) drainage trenching outside railway carriage or compartment: the degree of depth in gutter is 25cm, and face, railway carriage or compartment is anhydrous, ditch depth of water 5-20cm.
Use said method that Fructus Colocasiae Esculentae tissue cultured seedling or Horse hoof tissue cultured seedling are heeled in, heel in speed fast, within 20 days, can survive,
Within 40 days, i.e. may migrate to land for growing field crops, become live time short, survival rate is high, is difficult to long weeds, and cost of labor is low.
Compared with prior art, the invention have the benefit that
The substrate that the method for a kind of seedling of plant through temporary planting grouped cultivated that the present invention provides uses mainly, Ni Heshui fertile by dreg fermentation
Composition, this substrate is useful to plant tissue culture Seedling growth promoter, and substrate is in a liquid state, and plant tissue culture Seedling is directly put into rice seedling bed by workman
In, not blinding of not digging, heel in speed fast, be greatly saved cost of labor；Secondly the preparation of substrate is simple, dreg
Fermentation fertilizer and mud physical mixed, then pass to water, and dreg fermentation fertilizer includes abundant organic matter, it is possible to promote plant tissue culture
Seedling healthy growth；Again due to substrate is liquid, it is not likely to produce weeds, decreases the cost of artificial weeding, use said method
Plant survival rate after heeling in is up to 98%, and saving of labor more than 70%.
The dreg fermentation fertilizer of the present invention uses Auricularia dreg or the fermentation of Lentinus Edodes dreg to prepare, and Auricularia dreg or Lentinus Edodes dreg exist
Under the effect of leaven, changing into organic ratio high, dreg utilization rate is high, secondly Auricularia dreg fermentation fertilizer or Lentinus Edodes dreg
The granule of fermentation fertilizer is relatively thick, good permeability；The consumption fertile when dreg fermentation is 1.2-1.5kg/m2Time, the organic matter in substrate contains
Amount is optimal, will not be because fertility deficiency causes plant tissue culture Seedling dysplasia or produces the phenomenon of burning root because fertility is excessive；Play railway carriage or compartment energy
Enough realizations are heeled on a large scale；Hide plant tissue culture Seedling it can be avoided that plant tissue culture Seedling is tanned severely by high light with film or sunshade net, enter one
Step improves survival rate；Outside railway carriage or compartment, water unnecessary in railway carriage or compartment can timely and effective be discharged by drainage trenching, it is to avoid plant tissue culture Seedling is flooded；Adopt
Plant tissue culture Seedling in aforementioned manners can survive for 20 days, within 40 days, i.e. may migrate to land for growing field crops, and live time is short, survival rate is high for one-tenth.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but those skilled in the art will
Understanding, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.In embodiment unreceipted specifically
Condition person, the condition advised according to normal condition or manufacturer is carried out.
A kind of method that the invention provides seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by plant tissue culture Seedling seedling exercising；
(2) clean: take out the plant tissue culture Seedling after seedling exercising, sterilization, then clean；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, the substrate in rice seedling bed mainly fertile by dreg fermentation,
Mud and water composition.
The substrate that said method uses mainly, mud fertile by dreg fermentation and water form, and plant tissue culture Seedling is grown by this substrate
Growing useful, and substrate is in a liquid state, plant tissue culture Seedling is directly put in rice seedling bed by workman, and speed heeled in by not blinding of not digging
Degree is fast, is greatly saved cost of labor；Secondly the preparation of substrate is simple, dreg fermentation fertilizer and mud physical mixed, then leads to
Entering water, dreg fermentation fertilizer includes abundant organic matter, it is possible to promote plant tissue culture Seedling healthy growth；Again due to substrate is
Liquid, is not likely to produce weeds, decreases the cost of artificial weeding, and the plant survival rate after using said method to heel in is up to
98%, saving of labor more than 70%.
Below in conjunction with embodiment and detailed description of the invention, the present invention is further detailed explanation.
Embodiment 1
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Fructus Colocasiae Esculentae training tissue culture seedling 2 days；
(2) clean: take out the plant tissue culture Seedling after seedling exercising, wash with the strong chlorine oil sterilization of mass concentration 0.2%, then clear water
Only；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, and the substrate in rice seedling bed is mainly fermented by Auricularia dreg
Fertilizer, mud and water composition.
Embodiment 2
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Horse hoof tissue cultured seedling outdoor scattered light lower refining seedling 1 day, form plant tissue culture Seedling；
(2) cleaning: take out plant tissue culture Seedling, the strong chlorine oil aqueous solution using mass concentration to be 0.1% carries out disinfection, then
Clean with clear water；
(3) heel in: plant tissue culture Seedling is implanted in rice seedling bed, mainly, the mud fertile by the fermentation of Lentinus Edodes dreg of the substrate in rice seedling bed and
Water forms；Bottom surface elder generation cloth good Lentinus Edodes dreg fermentation fertilizer in rice seedling bed, makes fertile and on bottom surface, rice seedling bed the mud of dreg fermentation be mutually mixed,
The consumption of Lentinus Edodes dreg fermentation fertilizer is 1.2kg/m2, then pass to water, soak 10 days；The height of bottom surface, water surface distance rice seedling bed is
7cm；The fermentation of Lentinus Edodes dreg is fertile to be prepared by Lentinus Edodes dreg and fermentation treasured fermentation, and fermentation time is 28 days；Heeling in of plant tissue culture Seedling
Density is spacing in the rows 12cm, line-spacing 12cm, and the temperature heeled in is 20 DEG C, and the humidity heeled in is 93%.
Embodiment 3
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Fructus Colocasiae Esculentae tissue cultured seedling seedling exercising 2 days in the seedling growth greenhouse by 70% shading rate, seedling exercising temperature is 25 DEG C；
(2) cleaning: take out the plant tissue culture Seedling after seedling exercising, the strong chlorine oil aqueous solution using mass concentration to be 0.2% is carried out
Sterilize, then clean with clear water；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, and the substrate in rice seedling bed is mainly fermented by Auricularia dreg
Fertilizer, mud and water composition；Bottom surface elder generation cloth good Auricularia dreg fermentation fertilizer in rice seedling bed, makes the mud phase on dreg fermentation fertilizer and bottom surface, rice seedling bed
Mixing mutually, the consumption of Auricularia dreg fermentation fertilizer is 1.3kg/m2, then pass to water, soak 15 days；Bottom surface, water surface distance rice seedling bed
It is highly 8cm；The fertile fermentation by Auricularia dreg and Junyikang of Auricularia dreg fermentation prepares, and fermentation time is 30 days；Plant tissue culture Seedling
Density of heeling in be spacing in the rows 15cm, line-spacing 15cm, the temperature heeled in is 24 DEG C, and the humidity heeled in is 95%；
(4) railway carriage or compartment is played: play a width of 1m in railway carriage or compartment in railway carriage or compartment；
(5) plant tissue culture Seedling is covered with thin film；
(6) drainage trenching outside railway carriage or compartment: the degree of depth in gutter is 25cm, the width in gutter is 42cm.
Embodiment 4
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Horse hoof tissue cultured seedling seedling exercising 2 days in the seedling growth greenhouse by 70% shading rate, seedling exercising temperature is 20 DEG C；
(2) cleaning: take out the plant tissue culture Seedling after seedling exercising, the strong chlorine oil aqueous solution using mass concentration to be 0.3% is carried out
Sterilize, then clean with clear water；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, and the substrate in rice seedling bed is mainly fermented by Auricularia dreg
Fertilizer, mud and water composition；Bottom surface elder generation cloth good Auricularia dreg fermentation fertilizer in rice seedling bed, makes the mud phase on dreg fermentation fertilizer and bottom surface, rice seedling bed
Mixing mutually, the consumption of Auricularia dreg fermentation fertilizer is 1.4kg/m2, then pass to water, soak 20 days；Bottom surface, water surface distance rice seedling bed
It is highly 10cm；The fertile fermentation by Auricularia dreg and agriculture Citroen zx of Auricularia dreg fermentation prepares, and fermentation time is 34 days；Plant tissue culture Seedling
Density of heeling in be spacing in the rows 18cm, line-spacing 18cm, the temperature heeled in is 28 DEG C, and the humidity heeled in is 97%；
(4) railway carriage or compartment is played: play a width of 1.1m in railway carriage or compartment in railway carriage or compartment；
(5) plant tissue culture Seedling is covered with thin film and the sunshade net that shading rate is 70%；
(6) drainage trenching outside railway carriage or compartment: the degree of depth in gutter is 30cm, the width in gutter is 44cm.
Comparative example 1
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Fructus Colocasiae Esculentae training tissue culture seedling 2 days；
(2) clean: take out the plant tissue culture Seedling after seedling exercising, wash with the strong chlorine oil sterilization of mass concentration 0.2%, then clear water
Only；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in seedbed, and the substrate in seedbed uses the Vermiculitum of 1:1:1, mud
Charcoal soil and perlite are made.
Comparative example 2
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Horse hoof tissue cultured seedling outdoor scattered light lower refining seedling 3 days；
(2) cleaning: take out the plant tissue culture Seedling after seedling exercising, the strong chlorine oil aqueous solution using mass concentration to be 0.1% is carried out
Sterilize, then clean with clear water；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, and the substrate in rice seedling bed is mainly fermented by Lentinus Edodes dreg
Fertilizer, mud and water composition；Bottom surface elder generation cloth good Lentinus Edodes dreg fermentation fertilizer in rice seedling bed, makes the mud phase on dreg fermentation fertilizer and bottom surface, rice seedling bed
Mixing mutually, the consumption of Lentinus Edodes dreg fermentation fertilizer is 1kg/m2, then pass to water, soak 10 days；The height of bottom surface, water surface distance rice seedling bed
Degree is 7cm；The fermentation of Lentinus Edodes dreg is fertile to be prepared by Lentinus Edodes dreg and fermentation treasured fermentation, and fermentation time is 15 days；Plant tissue culture Seedling
Heeling in density is spacing in the rows 22cm, line-spacing 22cm, and the temperature heeled in is 20 DEG C, and the humidity heeled in is 93%.
Comparative example 3
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Fructus Colocasiae Esculentae tissue cultured seedling seedling exercising 2 days in the seedling growth greenhouse by 50% shading rate, seedling exercising temperature is 25 DEG C；
(2) cleaning: take out the plant tissue culture Seedling after seedling exercising, the strong chlorine oil aqueous solution using mass concentration to be 0.2% is carried out
Sterilize, then clean with clear water；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, and the substrate in rice seedling bed is mainly fermented by Auricularia dreg
Fertilizer, mud and water composition；Bottom surface elder generation cloth good Auricularia dreg fermentation fertilizer in rice seedling bed, makes the mud phase on dreg fermentation fertilizer and bottom surface, rice seedling bed
Mixing mutually, the consumption of Auricularia dreg fermentation fertilizer is 2kg/m2, then pass to water, soak 15 days；The height of bottom surface, water surface distance rice seedling bed
Degree is 15cm；The fertile fermentation by Auricularia dreg and Junyikang of Auricularia dreg fermentation prepares, and fermentation time is 45 days；Plant tissue culture Seedling
Heeling in density is spacing in the rows 15cm, line-spacing 15cm, and the temperature heeled in is 24 DEG C, and the humidity heeled in is 95%；
(4) railway carriage or compartment is played: play a width of 1.5m in railway carriage or compartment in railway carriage or compartment；
(5) plant tissue culture Seedling is covered with thin film；
(6) drainage trenching outside railway carriage or compartment: the degree of depth in gutter is 45cm, the width in gutter is 42cm.
Comparative example 4
A kind of method of seedling of plant through temporary planting grouped cultivated, comprises the following steps:
(1) seedling exercising: by Horse hoof tissue cultured seedling seedling exercising 1 day in the seedling growth greenhouse by 80% shading rate, seedling exercising temperature is 20 DEG C；
(2) cleaning: take out the plant tissue culture Seedling after seedling exercising, the strong chlorine oil aqueous solution using mass concentration to be 0.4% is carried out
Sterilize, then clean with clear water；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, and the substrate in rice seedling bed is mainly fermented by Auricularia dreg
Fertilizer, mud and water composition；Bottom surface elder generation cloth good Auricularia dreg fermentation fertilizer in rice seedling bed, makes the mud phase on dreg fermentation fertilizer and bottom surface, rice seedling bed
Mixing mutually, the consumption of Auricularia dreg fermentation fertilizer is 1.7kg/m2, then pass to water, soak 20 days；Bottom surface, water surface distance rice seedling bed
It is highly 10cm；The fertile fermentation by Auricularia dreg and agriculture Citroen zx of Auricularia dreg fermentation prepares, and fermentation time is 34 days；Plant tissue culture Seedling
Density of heeling in be spacing in the rows 18cm, line-spacing 18cm, the temperature heeled in is 28 DEG C, and the humidity heeled in is 85%；
(4) railway carriage or compartment is played: play a width of 1m in railway carriage or compartment in railway carriage or compartment；
(5) plant tissue culture Seedling is covered with thin film and the sunshade net that shading rate is 50%；
(6) drainage trenching outside railway carriage or compartment: the degree of depth in gutter is 30cm, the width in gutter is 44cm.
Fructus Colocasiae Esculentae tissue cultured seedling or Horse hoof tissue cultured seedling are heeled in, is divided into 8 groups, the Fructus Colocasiae Esculentae group training that often 1000 growing states of group are identical
Seedling or Horse hoof tissue cultured seedling, it is heeled in by the method being respectively adopted embodiment 1-4 and comparative example 1-4, and to plantation speed, one-tenth
Live time, survival rate and cost of labor carry out record, wherein plantation speed and cost of labor all on the basis of embodiment 1 in proportion
Being calculated, result is as shown in table 1.
Table 1 embodiment 1-4 and comparative example 1-4 heel in log
The comparing result of embodiment 1 and comparative example 1 understands, and the method plantation speed using the present invention to provide is fast, because right
Ratio 1 uses traditional solid state substrate, and need in planting process to dig and blinding etc. operates, and embodiment 1 uses this
Liquid matrix in bright, directly implants plant tissue culture Seedling in liquid matrix during plantation, and embodiment 1 is not likely to produce weeds,
Comparative example 1 is easily generated weeds, and therefore artificial weeding cost and the planting cost of embodiment 1 are low, and survival rate is high, become live time
Short, within 17 days, can survive.Comparative example 2 is compared with embodiment 2, and the consumption that the seedling exercising time is longer, the fermentation of Lentinus Edodes dreg is fertile is relatively low, strain
Away from relatively big with line-spacing, so the absorbent nutrition of every plant tissue culture Seedling institute is less, the therefore plant survival time in comparative example 2
Longer, survival rate is relatively low, cost of labor is higher comparatively speaking.Comparative example 3 is compared with embodiment 3, shading rate during seedling exercising is relatively low,
The consumption of Auricularia dreg fermentation fertilizer is higher, and when shading rate is relatively low, during seedling exercising, plant tissue culture Seedling is difficult to raw by strong illumination
Root survives, and the highest phenomenons such as plant tissue culture Seedling appearance burning root that cause of consumption that the fermentation of Auricularia dreg is fertile, the plant survival time is more
Long, reduce the survival rate of plant, the most relatively costly.Comparative example 4 is compared with embodiment 4, the strong chlorine oil used by sterilization
The consumption that the mass concentration of aqueous solution is higher, the fermentation of Auricularia dreg is fertile is higher, it is relatively low to heel in humidity and the shading rate of sunshade net relatively
Low, therefore the plant in comparative example 4 occurs in that blade is impaired and burns the phenomenons such as root, and the plant survival time is longer, plant survival rate
Relatively low, the most relatively costly.
By above comparing result, embodiment 1-4 is in plantation speed, one-tenth live time, survival rate and cost of labor side
Face is superior to or equal to comparative example 1-4, makes discovery from observation in addition, uses the method for embodiment 1-4 to produce without weeds, this is described
The method inventing the seedling of plant through temporary planting grouped cultivated provided has plantation speed generation weeds fast, difficult, cost of labor is low, become live time short
The advantage high with survival rate.
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that without departing substantially from the present invention's
May be made that in the case of spirit and scope many other change and amendment.It is, therefore, intended that in the following claims
Including all such changes and modifications belonged in the scope of the invention.
Claims (10)
1. the method for a seedling of plant through temporary planting grouped cultivated, it is characterised in that comprise the following steps:
(1) seedling exercising: by plant tissue culture Seedling seedling exercising；
(2) clean: take out the plant tissue culture Seedling after seedling exercising, sterilization, then clean；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, mainly, the mud fertile by dreg fermentation of the substrate in rice seedling bed and
Water forms.
Method the most according to claim 1, it is characterised in that the bottom surface elder generation cloth good dreg fermentation fertilizer in described rice seedling bed, makes bacterium
Fertile and on bottom surface, rice seedling bed the mud of slag fermentation is mutually mixed, and then passes to water, soaks 7-30 days；
Preferably, described dreg fermentation fertilizer is prepared by dreg and leaven, and fermentation time is 20-40 days；
Preferably, the height of bottom surface, water surface distance rice seedling bed is 5-10cm, preferably 8cm.
Method the most according to claim 2, it is characterised in that the consumption of described dreg fermentation fertilizer is 1.2-1.5kg/m2。
Method the most according to claim 2, it is characterised in that described dreg is edible fungi residue；
Preferably, described dreg is Auricularia dreg or Lentinus Edodes dreg.
Method the most according to claim 1, it is characterised in that the density of heeling in of described plant tissue culture Seedling is: spacing in the rows 10-
20cm, line-spacing 10-20cm；
Preferably, the density of heeling in of described plant tissue culture Seedling is: spacing in the rows 15cm, line-spacing 15cm；
Preferably, the temperature heeled in described in is 15-35 DEG C；
Preferably, the humidity heeled in described in is 90%-98%.
Method the most according to claim 1, it is characterised in that described plant tissue culture Seedling is Fructus Colocasiae Esculentae tissue cultured seedling or the training of Horse hoof group
Seedling.
Method the most according to claim 1, it is characterised in that described seedling exercising is in outdoor scattered light or has 70% shading rate
Carrying out in seedling growth greenhouse, seedling exercising temperature is 15-35 DEG C, and the seedling exercising time is 1-2 days；
Described sterilization is that the strong chlorine oil aqueous solution using mass concentration to be 0.1%-0.3% carries out disinfection.
Method the most according to claim 1, it is characterised in that described method is further comprising the steps of:
(4) railway carriage or compartment is played；
(5) described plant tissue culture Seedling is hidden with film and/or sunshade net；
(6) drainage trenching outside railway carriage or compartment.
Method the most according to claim 8, it is characterised in that a width of 0.8-1.2m in railway carriage or compartment in described railway carriage or compartment, described gutter
The degree of depth be 20-35cm, the width in described gutter is 40-45cm, and the shading rate of described sunshade net is 70%-75%；
Preferably, the degree of depth in described gutter is 25cm.
Method the most according to claim 1, it is characterised in that the method for described seedling of plant through temporary planting grouped cultivated includes following step
Rapid:
(1) seedling exercising: by Fructus Colocasiae Esculentae tissue cultured seedling or Horse hoof tissue cultured seedling outdoor scattered light or have 70% shading rate seedling growth greenhouse in carry out
Seedling exercising, seedling exercising temperature is 15-35 DEG C, seedling exercising 1-2 days；
(2) cleaning: take out the plant tissue culture Seedling after seedling exercising, the strong chlorine oil aqueous solution using mass concentration to be 0.2% carries out disinfection,
Then clean with clear water；
(3) heel in: the plant tissue culture Seedling after cleaning is implanted in rice seedling bed, mainly, the mud fertile by dreg fermentation of the substrate in rice seedling bed and
Water forms, and the good consumption of bottom surface elder generation cloth in rice seedling bed is 1.2-1.5kg/m2Dreg fermentation fertilizer, make dreg fermentation fertile and bottom surface, rice seedling bed
On mud be mutually mixed, then pass to water, soak 7-30 days；The height of bottom surface, water surface distance rice seedling bed is 8cm, plant tissue culture Seedling
Heeling in density is spacing in the rows 15cm, line-spacing 15cm, and the temperature heeled in is 25 DEG C, and the humidity heeled in is 95%；Described dreg fermentation fertilizer
Being prepared by Auricularia dreg or Lentinus Edodes dreg and leaven, fermentation time is 20-40 days；
(4) railway carriage or compartment is played: play a width of 1m in railway carriage or compartment in railway carriage or compartment；
(5) described plant tissue culture Seedling is hidden with film and/or sunshade net: the shading rate of sunshade net is 70%-75%；
(6) drainage trenching outside railway carriage or compartment: the degree of depth in gutter is 25cm, and face, railway carriage or compartment is anhydrous, ditch depth of water 5-20cm.
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| CN110384044A (en) * | 2019-08-16 | 2019-10-29 | 江西农业大学 | The breeding method of one ganoid konjaku taro detoxification seedling stem |
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