CN110384044A - The breeding method of one ganoid konjaku taro detoxification seedling stem - Google Patents

The breeding method of one ganoid konjaku taro detoxification seedling stem Download PDF

Info

Publication number
CN110384044A
CN110384044A CN201910756225.2A CN201910756225A CN110384044A CN 110384044 A CN110384044 A CN 110384044A CN 201910756225 A CN201910756225 A CN 201910756225A CN 110384044 A CN110384044 A CN 110384044A
Authority
CN
China
Prior art keywords
stem
seedling
detoxification
seedling stem
taro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201910756225.2A
Other languages
Chinese (zh)
Other versions
CN110384044B (en
Inventor
周庆红
朱强龙
单楠
黄英金
刘星月
方加军
周四红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangxi Agricultural University
Original Assignee
Jiangxi Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangxi Agricultural University filed Critical Jiangxi Agricultural University
Priority to CN201910756225.2A priority Critical patent/CN110384044B/en
Publication of CN110384044A publication Critical patent/CN110384044A/en
Application granted granted Critical
Publication of CN110384044B publication Critical patent/CN110384044B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses the breeding methods of a ganoid konjaku taro detoxification seedling stem, are related to taro detoxic seedling and cultivate field, specific breeding method is as follows: preparing culture medium using 6-BA, NAA, agar and sucrose, and places it in test tube;Stem apex is cut after the healthy and strong bulb vernalization of selected shape rule, stem apex is then inoculated into Fiber differentiation in stem apex growth medium, detoxification test tube plantlet is formed, test tube seedling is connected in bulb induced medium and obtains detoxification seedling stem;Detoxification seedling stem is transplanted and carries out hardening into turf+vermiculite matrix, during culture, observes and records taro growing state.The present invention by cultivating seedling stem using detoxification test tube plantlet in the medium, obtain detoxification seedling stem, then obtained detoxification seedling stem is transplanted into the hardening into turf+vermiculite matrix again, the induction of taro detoxification seedling stem, plantation survival rate can be effectively increased, reduces in test tube seedling planting process and breaks up serious situation, and seedling stem high yield, and easy to maintain, transport provides guarantee to the production of later period ganoid konjaku taro.

Description

The breeding method of one ganoid konjaku taro detoxification seedling stem
Technical field
The present invention relates to taro detoxic seedling Cultivating techniques field, it is more particularly related to a ganoid konjaku taro detoxification seedling stem Breeding method.
Background technique
According to studying for a long period of time for scientist, the reason of crops quality fails mainly plant virus infection.To obtain High yield must just turn out virus-free nursery stock, i.e. detoxification nursery stock in production, and here it is the concepts of detoxic seedling.The agriculture section in China Skilled worker author obtains the nursery stock material of virus-free plantation using biotechnology measure from the plant of virus infection.Such as Ma Ling The little or no virus of the apical meristem of potato plant obtains disease-free plant frequently with apical meristem culture technique, i.e., Toxicity-removing white potato.Make original seed with the potato wedge that this plant is born, breed more virus-free potato seeds, is pushed away for large area in production It is wide to use;Detoxic seedling technology has also applied in the agricultural productions such as flowers, fruit tree, konjaku now, to produce high-quality nontoxic Flowers ball kind, virus-free fruit tree nursery stock etc..
Taro detoxic seedling being obtained by taro stem-apex Meristem culture at present, but detoxification shoot survival percent is low, transport is inconvenient, And easily occurring tufted seedling after plantation, seedling stem low output has seriously affected the popularization of taro detoxic seedling.
The patent of invention of patent application publication CN208081437U discloses a kind of taro with red buds virus-free seedling tissue culture numerous skill fastly Art is to choose the healthy and strong, bulb without disease pest, is sterilized, sterilized, the stem apex of picking 0.2mm size is inoculated into induced medium Carry out differentiation culture;After being trained Multiple Buds, Multiple Buds top is wiped out, it is fast numerous to be put into progress in proliferated culture medium;When growing thickly When bud bud-leaf is unfolded, it is put into progress strengthening seedling and rooting culture in root media, is then transferred to hardening.This method uses in breeding Detoxification technology twice, picking shoop-tips, improves detoxification efficiency.Formula is optimized in culture medium preparation, is conducive to test tube Seedling growth.Traditional drawback for using bulb big, at high cost as propagation material sowing quantity is overcome, control culture that can be artificial is given birth to Elongate member is not influenced by natural conditions, and materials amount is small, and culture materials economy, growth cycle is short, and breeding coefficient is big, is reduced Production cost maintains Different Varieties, improves yield and quality, it can be achieved that industrial seedling rearing and industrialization production.
But still there is more disadvantage in practice in above-mentioned technical proposal, the detoxic seedling such as obtained is not easy to maintain, Transport is inconvenient, causes to occur detoxic seedling mortality in transportational process and the later period is not easy the phenomenon that surviving, and hold after planting Easily differentiation, can not ensure the production of later period ganoid konjaku taro.
Summary of the invention
In order to overcome the drawbacks described above of the prior art, the embodiment of the present invention provides the cultivation side of a ganoid konjaku taro detoxification seedling stem Method, by utilizing the culture medium culture detoxification test tube plantlet of+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar, Test tube seedling stem is obtained, then obtained test tube seedling stem is transplanted into turf+vermiculite matrix again and is cultivated again, taro detoxification The induction of seedling stem can effectively increase taro plantation survival rate, reduce differentiation, and seedling stem high yield, and detoxification seedling stem is easy to maintain, just Break up after transport, plantation less, guarantee is provided to the production of later period ganoid konjaku taro.
To achieve the above object, the invention provides the following technical scheme: the breeding method of a ganoid konjaku taro detoxification seedling stem, specific to train It is as follows to educate method:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA, NAA, agar and sucrose, it is spare after high pressure sterilization after mixing;
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.5-2cm when, under the microscope The stem apex that taro simple bud is about 0.2-0.3mm is cut, stem apex is then inoculated into Fiber differentiation in culture medium, forms detoxicating cuvette Seedling;
S3, test tube seedling obtained in S2 is connected in seedling stem induced medium and is cultivated, obtain detoxification seedling stem;
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3 Seedling stem, which is transplanted, carries out hardening into turf+vermiculite matrix, during culture, observes and records taro seedling stem growing state.
In a preferred embodiment, in the step S1, Stem tip induction culture medium is specially MS+2mg/L6-BA+ + 3% sucrose of 0.5mg/LNAA+7g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S1, seedling stem induced medium is specially MS+0.8mg/L6- + 6% sucrose of BA+0.4mg/LNAA+6.8g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S1, seedling stem induced medium is specially MS+1.0mg/L6- + 8% sucrose of BA+0.5mg/LNAA+7g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S1, seedling stem induced medium is specially MS+1.2mg/L6- + 10% sucrose of BA+0.6mg/LNAA+7.2g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S3, seedling stem cultivation temperature is 20-30 DEG C.
In a preferred embodiment, in the step S3, the intensity of illumination of seedling stem Fiber differentiation is 4000lx, light It is 13-15h/d according to the time.
In a preferred embodiment, in the step S4, the ratio of turf and vermiculite is 1-3:1.
Technical effect and advantage of the invention:
The present invention passes through the culture medium progress using+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar Seedling stem is cultivated, detoxification seedling stem is obtained, then obtained detoxification seedling stem is transplanted again and carries out hardening into turf+vermiculite matrix, The induction of taro detoxification seedling stem can effectively increase plantation survival rate, reduce differentiation, and seedling stem high yield, and detoxification seedling stem is easily protected It deposits, is readily transported, it is undifferentiated after plantation, guarantee is provided to the production of later period ganoid konjaku taro.
Specific embodiment
Below in conjunction with the embodiment in the present invention, technical solution in the embodiment of the present invention is carried out clearly and completely Description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on this hair Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts Example, shall fall within the protection scope of the present invention.
Embodiment 1:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA, NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment PH is 5.7, and seedling stem induced medium is specially+6% sucrose of MS+0.8mg/L6-BA+0.4mg/LNAA+6.8g/L agar, mixing After obtain culture medium, adjustment pH is 5.7, and obtained culture medium is spare after high pressure sterilization;
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.5cm when, cut under the microscope It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into culture medium Fiber differentiation into detoxic seedling;
S3, detoxic seedling is connected in seedling stem induced medium and is cultivated, cultivation temperature is 20 DEG C, the illumination of seedling stem Fiber differentiation Intensity is 4000lx, and light application time 13h/d obtains detoxification seedling stem;
S4, turf and vermiculite are mixed according to a certain percentage, obtains transplanting medium, by detoxification seedling stem obtained in step S3 It transplants and carries out hardening into turf+vermiculite matrix, the ratio of turf and vermiculite is 2:1, during culture, observes and records taro seedling stem Growing state.
Embodiment 2:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and bulb induced medium, be added in minimal medium MS certain mass 6-BA, NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment PH is 5.7, and seedling stem induced medium is specially+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar, after mixing Culture medium is obtained, adjustment pH is 5.7, spare after obtained culture medium high pressure sterilization;
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.7cm when, cut under the microscope It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into culture medium Fiber differentiation into test tube seedling;
S3, S2 test tube seedling is connected in seedling stem induced medium, seedling stem cultivation temperature is 25 DEG C, and intensity of illumination is 4000lx, light application time 14h/d obtain detoxification seedling stem;
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3 Seedling stem, which is transplanted into turf+vermiculite matrix, carries out hardening, and the ratio of turf and vermiculite is 2:1, during culture, observes and records taro Seedling stem growing state.
Embodiment 3:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and bulb induced medium, be added in minimal medium MS certain mass 6-BA, NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment PH is 5.7, and seedling stem induced medium is specially+10% sucrose of MS+1.2mg/L6-BA+0.6mg/LNAA+7.2g/L agar, is adjusted Whole pH is 5.7, obtains culture medium after mixing, spare after obtained culture medium high pressure sterilization;
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 2cm when, cut under the microscope Taro simple bud is about the stem apex of 0.2-0.3mm, and stem apex is then inoculated into culture medium Fiber differentiation into detoxic seedling;
S3, detoxic seedling obtained in S2 is connected in seedling stem induced medium, cultivation temperature is 30 DEG C, the light of Fiber differentiation It is 4000lx according to intensity, light application time 15h/d obtains detoxification seedling stem;
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3 Seedling stem, which is transplanted into turf+vermiculite matrix, carries out hardening, and the ratio of turf and vermiculite is 2:1, during culture, observes and records kind Stem nematode situation.
Embodiment 4:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA, NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment PH is 5.7, and seedling stem induced medium is specially+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar, after mixing Culture medium is obtained, adjustment pH is 5.7, spare after obtained culture medium high pressure sterilization;
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.7cm when, cut under the microscope It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into culture medium Fiber differentiation into detoxic seedling;
S3, detoxic seedling obtained in S2 is connected in seedling stem induced medium, cultivation temperature is 25 DEG C, the light of Fiber differentiation It is 4000lx according to intensity, light application time 14h/d obtains detoxification seedling stem;
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3 Seedling stem, which is transplanted into turf+vermiculite matrix, carries out hardening, and the ratio of turf and vermiculite is 1:1, during culture, observes and records taro Seedling stem growing state.
Embodiment 5:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA, NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment PH is 5.7, and seedling stem induced medium is specially+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar, after mixing Culture medium is obtained, adjustment pH is 5.7, spare after obtained culture medium high pressure sterilization;
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.7cm when, cut under the microscope It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into the culture medium in test tube Fiber differentiation into detoxic seedling;
S3, detoxic seedling obtained in S2 is connected in seedling stem induced medium, cultivation temperature is 25 DEG C, the light of Fiber differentiation It is 4000lx according to intensity, light application time 14h/d obtains detoxification seedling stem;
S4, turf and vermiculite are mixed according to a certain percentage, obtains transplanting medium, by test tube seedling stem obtained in step S3 Transplanting is cultivated again into turf+vermiculite matrix, and the ratio of turf and vermiculite is 3:1, during culture, observes and records kind Stem nematode situation.
Embodiment 6:
The growth data of 30 taro detoxification seedling stems that above-described embodiment 1-5 is cultivated in the training period is randomly selected, is obtained Below table:
As seen from the above table, culture medium and transplanting medium chinese raw materials ratio are moderate in embodiment 2, and the temperature in incubation Degree and illumination are all proper, are conducive to the culture of taro detoxification seedling stem, obtained taro detoxic seedling rooting rate and survival rate is all significantly It improves, breaks up after plantation few.
Last: the foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, all in the present invention Spirit and principle within, any modification, equivalent replacement, improvement and so on, should be included in protection scope of the present invention it It is interior.

Claims (8)

1. the breeding method of a ganoid konjaku taro detoxification seedling stem, it is characterised in that: specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, are added 6-BA, NAA, the agar of certain mass in MS culture medium And sucrose, it is spare after high pressure sterilization after mixing;
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.5-2cm when, cut under the microscope Taro simple bud is about the stem apex of 0.2-0.3mm, and stem apex is then inoculated into Fiber differentiation in Shoot Tip Culture base, forms detoxicating cuvette Seedling;
S3, the test tube seedling in S2 is inoculated into seedling stem induced medium and is cultivated, obtain detoxification seedling stem;
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, then by seedling stem obtained in step S3 Transplanting carries out hardening into turf+vermiculite matrix, during culture, observes and records taro seedling stem growing state.
2. the breeding method of ganoid konjaku taro detoxic seedling according to claim 1, it is characterised in that: in the step S1, stem apex Culture medium is specially+3% sucrose of MS+2mg/L 6-BA+0.5mg/L NAA+7g/L agar, and adjustment pH is 5.7.
3. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S1, kind Stem induced medium is specially+6% sucrose of MS+0.8mg/L 6-BA+0.4mg/L NAA+6.8g/L agar, and adjustment pH is 5.7.
4. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S1, kind Stem induced medium is specially+8% sucrose of MS+1.0mg/L 6-BA+0.5mg/L NAA+7g/L agar, and adjustment pH is 5.7.
5. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S1, kind Stem induced medium is specially+10% sucrose of MS+1.2mg/L 6-BA+0.6mg/L NAA+7.2g/L agar, and adjustment pH is 5.7。
6. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S3, kind Stem cultivation temperature is 20-30 DEG C.
7. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S3, kind The intensity of illumination of stem Fiber differentiation is 4000lx, light application time 13-15h/d.
8. the breeding method of ganoid konjaku taro detoxic seedling according to claim 1, it is characterised in that: in the step S4, turf Ratio with vermiculite is 1-3:1.
CN201910756225.2A 2019-08-16 2019-08-16 Cultivation method of virus-free seed stems of taros Active CN110384044B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910756225.2A CN110384044B (en) 2019-08-16 2019-08-16 Cultivation method of virus-free seed stems of taros

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910756225.2A CN110384044B (en) 2019-08-16 2019-08-16 Cultivation method of virus-free seed stems of taros

Publications (2)

Publication Number Publication Date
CN110384044A true CN110384044A (en) 2019-10-29
CN110384044B CN110384044B (en) 2023-01-24

Family

ID=68288850

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910756225.2A Active CN110384044B (en) 2019-08-16 2019-08-16 Cultivation method of virus-free seed stems of taros

Country Status (1)

Country Link
CN (1) CN110384044B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112655562A (en) * 2021-01-14 2021-04-16 上饶师范学院 Method for promoting germination of test-tube taro of red-bud taro
CN113455365A (en) * 2021-08-02 2021-10-01 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) Method for preserving potted plant of common wild rice seed stems

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1179882A (en) * 1997-10-07 1998-04-29 南京农业大学 Rapid bulb propagation method for betel nut taro seeds
CN102217550A (en) * 2011-06-09 2011-10-19 马宗新 Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros
CN103070071A (en) * 2013-01-22 2013-05-01 武汉市蔬菜科学研究所 Method for fast propagation of test tube taro by detoxification
EP2927323A2 (en) * 2011-04-11 2015-10-07 Targeted Growth, Inc. Identification and the use of krp mutants in plants
CN106234229A (en) * 2016-09-30 2016-12-21 广西壮族自治区农业科学院 Method for temporarily planting taro tissue culture seedlings
USPP27733P2 (en) * 2015-02-10 2017-02-28 Brian's Botanicals Colocasia plant named ‘Painted Black Gecko’
CN111919747A (en) * 2020-07-27 2020-11-13 衡阳市蔬菜研究所 Method for improving inductivity and rooting rate based on detoxified areca taro culture

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1179882A (en) * 1997-10-07 1998-04-29 南京农业大学 Rapid bulb propagation method for betel nut taro seeds
EP2927323A2 (en) * 2011-04-11 2015-10-07 Targeted Growth, Inc. Identification and the use of krp mutants in plants
CN102217550A (en) * 2011-06-09 2011-10-19 马宗新 Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros
CN103070071A (en) * 2013-01-22 2013-05-01 武汉市蔬菜科学研究所 Method for fast propagation of test tube taro by detoxification
USPP27733P2 (en) * 2015-02-10 2017-02-28 Brian's Botanicals Colocasia plant named ‘Painted Black Gecko’
CN106234229A (en) * 2016-09-30 2016-12-21 广西壮族自治区农业科学院 Method for temporarily planting taro tissue culture seedlings
CN111919747A (en) * 2020-07-27 2020-11-13 衡阳市蔬菜研究所 Method for improving inductivity and rooting rate based on detoxified areca taro culture

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
MURAKAMI, KENJI等: "CALLUS FORMATION AND PLANT-REGENERATION FROM ETIOLATED STEM OF TARO (COLOCASIA-ESCULENTA SCHOTT", 《ENGEI GAKKAI ZASSHI》 *
刘星月等: "红芽芋脱毒试管芋诱导及植株再生", 《园艺学报》 *
刘玉平等: "试管芋诱导的研究", 《园艺学报》 *
赵建萍等: "日本芋品种茎尖离体快繁技术研究", 《中国种业》 *
郭克婷等: "张溪香芋茎尖组培快繁技术研究", 《广东农业科学》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112655562A (en) * 2021-01-14 2021-04-16 上饶师范学院 Method for promoting germination of test-tube taro of red-bud taro
CN112655562B (en) * 2021-01-14 2023-01-03 上饶师范学院 Method for promoting germination of test-tube taro of red-bud taro
CN113455365A (en) * 2021-08-02 2021-10-01 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) Method for preserving potted plant of common wild rice seed stems
CN113455365B (en) * 2021-08-02 2022-05-31 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) Method for preserving potted plant of common wild rice seed stems

Also Published As

Publication number Publication date
CN110384044B (en) 2023-01-24

Similar Documents

Publication Publication Date Title
CN101611697B (en) Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19'
CN103960134B (en) Method for producing sweet potato detoxified seedlings in water culture manner
CN102870680B (en) Efficient rapid propagation technique appropriate for detoxified rabbiteye blueberries
CN101491215B (en) Chinese toon tissue-culture quick propagation technique
CN102217550A (en) Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros
CN102047842A (en) Method for directly regenerating plants by adopting citrullus lanatus cotyledon nodes
CN103416304A (en) Method for cultivating water-saving and drought-resistant rice anther
CN110384044A (en) The breeding method of one ganoid konjaku taro detoxification seedling stem
CN106538382B (en) Method for establishing efficient eremochloa ophiuroides regeneration system by taking young ears as explants
CN103155869A (en) Sweet cherry rootstock Colt tissue culture method
CN1203754C (en) Breeding technology of test-tube konjac
CN103563747A (en) Detoxification and rapid-propagation method of huilou yam
CN108112479B (en) A kind of stem section of papaya sprout Bud Differentiation vacantly plants leaf promoting root growth method
CN103477976A (en) Stem tissue culture seedling method of dendrobium candidum
CN105010123B (en) The method and culture medium of strawberry distant hybrid are obtained by rescue isolated culture
JP2005168399A (en) Method for producing phalaenopsis clone seedling
CN100391333C (en) Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum
CN106613973B (en) Utilize the method for tissue-cultured seedling leaf regeneration adventitious bud fast breeding Chinese azalea
CN108401907A (en) A kind of Apples Dwarf Stocks M9T337 tissue cultures volume production method
CN101874471B (en) Plant regeneration method of dianthus caryophyllus direct somatic embryo generating path and special culture medium
CN104663445A (en) In-vitro rapid propagation technology of high lipid-producing pinus elliottii
CN104115751A (en) Culture method for obtaining regenerated plantlet by utilizing Chinese cabbage bulb leaves
CN1110250C (en) Fast seedling growing mehtod for Chinese flowering crabapple zhumei
CN113711914A (en) Caragana microphylla in-vitro regeneration method taking cotyledonary node as explant
KR100457999B1 (en) Propagation Method of Pinus rigida × P. taeda through Somatic Embryogenesis Technique

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant