CN110384044A - The breeding method of one ganoid konjaku taro detoxification seedling stem - Google Patents
The breeding method of one ganoid konjaku taro detoxification seedling stem Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses the breeding methods of a ganoid konjaku taro detoxification seedling stem, are related to taro detoxic seedling and cultivate field, specific breeding method is as follows: preparing culture medium using 6-BA, NAA, agar and sucrose, and places it in test tube；Stem apex is cut after the healthy and strong bulb vernalization of selected shape rule, stem apex is then inoculated into Fiber differentiation in stem apex growth medium, detoxification test tube plantlet is formed, test tube seedling is connected in bulb induced medium and obtains detoxification seedling stem；Detoxification seedling stem is transplanted and carries out hardening into turf+vermiculite matrix, during culture, observes and records taro growing state.The present invention by cultivating seedling stem using detoxification test tube plantlet in the medium, obtain detoxification seedling stem, then obtained detoxification seedling stem is transplanted into the hardening into turf+vermiculite matrix again, the induction of taro detoxification seedling stem, plantation survival rate can be effectively increased, reduces in test tube seedling planting process and breaks up serious situation, and seedling stem high yield, and easy to maintain, transport provides guarantee to the production of later period ganoid konjaku taro.
Description
Technical field
The present invention relates to taro detoxic seedling Cultivating techniques field, it is more particularly related to a ganoid konjaku taro detoxification seedling stem
Breeding method.
Background technique
According to studying for a long period of time for scientist, the reason of crops quality fails mainly plant virus infection.To obtain
High yield must just turn out virus-free nursery stock, i.e. detoxification nursery stock in production, and here it is the concepts of detoxic seedling.The agriculture section in China
Skilled worker author obtains the nursery stock material of virus-free plantation using biotechnology measure from the plant of virus infection.Such as Ma Ling
The little or no virus of the apical meristem of potato plant obtains disease-free plant frequently with apical meristem culture technique, i.e.,
Toxicity-removing white potato.Make original seed with the potato wedge that this plant is born, breed more virus-free potato seeds, is pushed away for large area in production
It is wide to use；Detoxic seedling technology has also applied in the agricultural productions such as flowers, fruit tree, konjaku now, to produce high-quality nontoxic
Flowers ball kind, virus-free fruit tree nursery stock etc..
Taro detoxic seedling being obtained by taro stem-apex Meristem culture at present, but detoxification shoot survival percent is low, transport is inconvenient,
And easily occurring tufted seedling after plantation, seedling stem low output has seriously affected the popularization of taro detoxic seedling.
The patent of invention of patent application publication CN208081437U discloses a kind of taro with red buds virus-free seedling tissue culture numerous skill fastly
Art is to choose the healthy and strong, bulb without disease pest, is sterilized, sterilized, the stem apex of picking 0.2mm size is inoculated into induced medium
Carry out differentiation culture；After being trained Multiple Buds, Multiple Buds top is wiped out, it is fast numerous to be put into progress in proliferated culture medium；When growing thickly
When bud bud-leaf is unfolded, it is put into progress strengthening seedling and rooting culture in root media, is then transferred to hardening.This method uses in breeding
Detoxification technology twice, picking shoop-tips, improves detoxification efficiency.Formula is optimized in culture medium preparation, is conducive to test tube
Seedling growth.Traditional drawback for using bulb big, at high cost as propagation material sowing quantity is overcome, control culture that can be artificial is given birth to
Elongate member is not influenced by natural conditions, and materials amount is small, and culture materials economy, growth cycle is short, and breeding coefficient is big, is reduced
Production cost maintains Different Varieties, improves yield and quality, it can be achieved that industrial seedling rearing and industrialization production.
But still there is more disadvantage in practice in above-mentioned technical proposal, the detoxic seedling such as obtained is not easy to maintain,
Transport is inconvenient, causes to occur detoxic seedling mortality in transportational process and the later period is not easy the phenomenon that surviving, and hold after planting
Easily differentiation, can not ensure the production of later period ganoid konjaku taro.
Summary of the invention
In order to overcome the drawbacks described above of the prior art, the embodiment of the present invention provides the cultivation side of a ganoid konjaku taro detoxification seedling stem
Method, by utilizing the culture medium culture detoxification test tube plantlet of+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar,
Test tube seedling stem is obtained, then obtained test tube seedling stem is transplanted into turf+vermiculite matrix again and is cultivated again, taro detoxification
The induction of seedling stem can effectively increase taro plantation survival rate, reduce differentiation, and seedling stem high yield, and detoxification seedling stem is easy to maintain, just
Break up after transport, plantation less, guarantee is provided to the production of later period ganoid konjaku taro.
To achieve the above object, the invention provides the following technical scheme: the breeding method of a ganoid konjaku taro detoxification seedling stem, specific to train
It is as follows to educate method:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA,
NAA, agar and sucrose, it is spare after high pressure sterilization after mixing；
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.5-2cm when, under the microscope
The stem apex that taro simple bud is about 0.2-0.3mm is cut, stem apex is then inoculated into Fiber differentiation in culture medium, forms detoxicating cuvette
Seedling；
S3, test tube seedling obtained in S2 is connected in seedling stem induced medium and is cultivated, obtain detoxification seedling stem；
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3
Seedling stem, which is transplanted, carries out hardening into turf+vermiculite matrix, during culture, observes and records taro seedling stem growing state.
In a preferred embodiment, in the step S1, Stem tip induction culture medium is specially MS+2mg/L6-BA+
+ 3% sucrose of 0.5mg/LNAA+7g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S1, seedling stem induced medium is specially MS+0.8mg/L6-
+ 6% sucrose of BA+0.4mg/LNAA+6.8g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S1, seedling stem induced medium is specially MS+1.0mg/L6-
+ 8% sucrose of BA+0.5mg/LNAA+7g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S1, seedling stem induced medium is specially MS+1.2mg/L6-
+ 10% sucrose of BA+0.6mg/LNAA+7.2g/L agar, adjustment PH are 5.7.
In a preferred embodiment, in the step S3, seedling stem cultivation temperature is 20-30 DEG C.
In a preferred embodiment, in the step S3, the intensity of illumination of seedling stem Fiber differentiation is 4000lx, light
It is 13-15h/d according to the time.
In a preferred embodiment, in the step S4, the ratio of turf and vermiculite is 1-3:1.
Technical effect and advantage of the invention:
The present invention passes through the culture medium progress using+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar
Seedling stem is cultivated, detoxification seedling stem is obtained, then obtained detoxification seedling stem is transplanted again and carries out hardening into turf+vermiculite matrix,
The induction of taro detoxification seedling stem can effectively increase plantation survival rate, reduce differentiation, and seedling stem high yield, and detoxification seedling stem is easily protected
It deposits, is readily transported, it is undifferentiated after plantation, guarantee is provided to the production of later period ganoid konjaku taro.
Specific embodiment
Below in conjunction with the embodiment in the present invention, technical solution in the embodiment of the present invention is carried out clearly and completely
Description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on this hair
Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
Embodiment 1:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA,
NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment
PH is 5.7, and seedling stem induced medium is specially+6% sucrose of MS+0.8mg/L6-BA+0.4mg/LNAA+6.8g/L agar, mixing
After obtain culture medium, adjustment pH is 5.7, and obtained culture medium is spare after high pressure sterilization；
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.5cm when, cut under the microscope
It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into culture medium Fiber differentiation into detoxic seedling；
S3, detoxic seedling is connected in seedling stem induced medium and is cultivated, cultivation temperature is 20 DEG C, the illumination of seedling stem Fiber differentiation
Intensity is 4000lx, and light application time 13h/d obtains detoxification seedling stem；
S4, turf and vermiculite are mixed according to a certain percentage, obtains transplanting medium, by detoxification seedling stem obtained in step S3
It transplants and carries out hardening into turf+vermiculite matrix, the ratio of turf and vermiculite is 2:1, during culture, observes and records taro seedling stem
Growing state.
Embodiment 2:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and bulb induced medium, be added in minimal medium MS certain mass 6-BA,
NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment
PH is 5.7, and seedling stem induced medium is specially+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar, after mixing
Culture medium is obtained, adjustment pH is 5.7, spare after obtained culture medium high pressure sterilization；
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.7cm when, cut under the microscope
It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into culture medium Fiber differentiation into test tube seedling；
S3, S2 test tube seedling is connected in seedling stem induced medium, seedling stem cultivation temperature is 25 DEG C, and intensity of illumination is
4000lx, light application time 14h/d obtain detoxification seedling stem；
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3
Seedling stem, which is transplanted into turf+vermiculite matrix, carries out hardening, and the ratio of turf and vermiculite is 2:1, during culture, observes and records taro
Seedling stem growing state.
Embodiment 3:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and bulb induced medium, be added in minimal medium MS certain mass 6-BA,
NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment
PH is 5.7, and seedling stem induced medium is specially+10% sucrose of MS+1.2mg/L6-BA+0.6mg/LNAA+7.2g/L agar, is adjusted
Whole pH is 5.7, obtains culture medium after mixing, spare after obtained culture medium high pressure sterilization；
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 2cm when, cut under the microscope
Taro simple bud is about the stem apex of 0.2-0.3mm, and stem apex is then inoculated into culture medium Fiber differentiation into detoxic seedling；
S3, detoxic seedling obtained in S2 is connected in seedling stem induced medium, cultivation temperature is 30 DEG C, the light of Fiber differentiation
It is 4000lx according to intensity, light application time 15h/d obtains detoxification seedling stem；
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3
Seedling stem, which is transplanted into turf+vermiculite matrix, carries out hardening, and the ratio of turf and vermiculite is 2:1, during culture, observes and records kind
Stem nematode situation.
Embodiment 4:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA,
NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment
PH is 5.7, and seedling stem induced medium is specially+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar, after mixing
Culture medium is obtained, adjustment pH is 5.7, spare after obtained culture medium high pressure sterilization；
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.7cm when, cut under the microscope
It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into culture medium Fiber differentiation into detoxic seedling；
S3, detoxic seedling obtained in S2 is connected in seedling stem induced medium, cultivation temperature is 25 DEG C, the light of Fiber differentiation
It is 4000lx according to intensity, light application time 14h/d obtains detoxification seedling stem；
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, by detoxification obtained in step S3
Seedling stem, which is transplanted into turf+vermiculite matrix, carries out hardening, and the ratio of turf and vermiculite is 1:1, during culture, observes and records taro
Seedling stem growing state.
Embodiment 5:
The present invention provides the breeding method of a ganoid konjaku taro detoxification seedling stem, specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, be added in minimal medium MS certain mass 6-BA,
NAA, agar and sucrose, Shoot Tip Culture base are specially+3% sucrose of MS+2mg/L6-BA+0.5mg/LNAA+7g/L agar, adjustment
PH is 5.7, and seedling stem induced medium is specially+8% sucrose of MS+1.0mg/L6-BA+0.5mg/LNAA+7g/L agar, after mixing
Culture medium is obtained, adjustment pH is 5.7, spare after obtained culture medium high pressure sterilization；
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.7cm when, cut under the microscope
It takes taro simple bud to be about the stem apex of 0.2-0.3mm, stem apex is then inoculated into the culture medium in test tube Fiber differentiation into detoxic seedling；
S3, detoxic seedling obtained in S2 is connected in seedling stem induced medium, cultivation temperature is 25 DEG C, the light of Fiber differentiation
It is 4000lx according to intensity, light application time 14h/d obtains detoxification seedling stem；
S4, turf and vermiculite are mixed according to a certain percentage, obtains transplanting medium, by test tube seedling stem obtained in step S3
Transplanting is cultivated again into turf+vermiculite matrix, and the ratio of turf and vermiculite is 3:1, during culture, observes and records kind
Stem nematode situation.
Embodiment 6:
The growth data of 30 taro detoxification seedling stems that above-described embodiment 1-5 is cultivated in the training period is randomly selected, is obtained
Below table:
As seen from the above table, culture medium and transplanting medium chinese raw materials ratio are moderate in embodiment 2, and the temperature in incubation
Degree and illumination are all proper, are conducive to the culture of taro detoxification seedling stem, obtained taro detoxic seedling rooting rate and survival rate is all significantly
It improves, breaks up after plantation few.
Last: the foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, all in the present invention
Spirit and principle within, any modification, equivalent replacement, improvement and so on, should be included in protection scope of the present invention it
It is interior.
Claims (8)
1. the breeding method of a ganoid konjaku taro detoxification seedling stem, it is characterised in that: specific breeding method is as follows:
S1, preparation Shoot Tip Culture base and seedling stem induced medium, are added 6-BA, NAA, the agar of certain mass in MS culture medium
And sucrose, it is spare after high pressure sterilization after mixing；
S2, choose vernalization behind healthy and strong, the bulb wash clean surface without disease pest, when bud it is long to 1.5-2cm when, cut under the microscope
Taro simple bud is about the stem apex of 0.2-0.3mm, and stem apex is then inoculated into Fiber differentiation in Shoot Tip Culture base, forms detoxicating cuvette
Seedling；
S3, the test tube seedling in S2 is inoculated into seedling stem induced medium and is cultivated, obtain detoxification seedling stem；
S4, turf and vermiculite are mixed according to a certain percentage, obtains seedling stem transplanting medium, then by seedling stem obtained in step S3
Transplanting carries out hardening into turf+vermiculite matrix, during culture, observes and records taro seedling stem growing state.
2. the breeding method of ganoid konjaku taro detoxic seedling according to claim 1, it is characterised in that: in the step S1, stem apex
Culture medium is specially+3% sucrose of MS+2mg/L 6-BA+0.5mg/L NAA+7g/L agar, and adjustment pH is 5.7.
3. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S1, kind
Stem induced medium is specially+6% sucrose of MS+0.8mg/L 6-BA+0.4mg/L NAA+6.8g/L agar, and adjustment pH is 5.7.
4. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S1, kind
Stem induced medium is specially+8% sucrose of MS+1.0mg/L 6-BA+0.5mg/L NAA+7g/L agar, and adjustment pH is 5.7.
5. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S1, kind
Stem induced medium is specially+10% sucrose of MS+1.2mg/L 6-BA+0.6mg/L NAA+7.2g/L agar, and adjustment pH is
5.7。
6. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S3, kind
Stem cultivation temperature is 20-30 DEG C.
7. the breeding method of ganoid konjaku taro detoxification seedling stem according to claim 1, it is characterised in that: in the step S3, kind
The intensity of illumination of stem Fiber differentiation is 4000lx, light application time 13-15h/d.
8. the breeding method of ganoid konjaku taro detoxic seedling according to claim 1, it is characterised in that: in the step S4, turf
Ratio with vermiculite is 1-3:1.
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| CN113455365A (en) * | 2021-08-02 | 2021-10-01 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for preserving potted plant of common wild rice seed stems |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112655562A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
| CN112655562B (en) * | 2021-01-14 | 2023-01-03 | 上饶师范学院 | Method for promoting germination of test-tube taro of red-bud taro |
| CN113455365A (en) * | 2021-08-02 | 2021-10-01 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for preserving potted plant of common wild rice seed stems |
| CN113455365B (en) * | 2021-08-02 | 2022-05-31 | 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) | Method for preserving potted plant of common wild rice seed stems |
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