CN1179882A - Rapid bulb propagation method for betel nut taro seeds - Google Patents
Rapid bulb propagation method for betel nut taro seeds Download PDFInfo
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- CN1179882A CN1179882A CN 97118966 CN97118966A CN1179882A CN 1179882 A CN1179882 A CN 1179882A CN 97118966 CN97118966 CN 97118966 CN 97118966 A CN97118966 A CN 97118966A CN 1179882 A CN1179882 A CN 1179882A
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 244000205754 Colocasia esculenta Species 0.000 title abstract description 12
- 235000006481 Colocasia esculenta Nutrition 0.000 title abstract description 12
- 235000006226 Areca catechu Nutrition 0.000 title abstract 2
- 244000080767 Areca catechu Species 0.000 title abstract 2
- 239000001963 growth medium Substances 0.000 claims abstract description 32
- 239000007787 solid Substances 0.000 claims abstract description 22
- 230000001939 inductive effect Effects 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000012360 testing method Methods 0.000 claims abstract description 8
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 35
- 238000000338 in vitro Methods 0.000 claims description 23
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 19
- 229930006000 Sucrose Natural products 0.000 claims description 19
- 239000005720 sucrose Substances 0.000 claims description 19
- 240000008154 Piper betle Species 0.000 claims description 14
- 235000008180 Piper betle Nutrition 0.000 claims description 14
- 238000009395 breeding Methods 0.000 claims description 14
- 230000001488 breeding effect Effects 0.000 claims description 14
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 239000005556 hormone Substances 0.000 claims description 4
- 229940088597 hormone Drugs 0.000 claims description 4
- 229930191978 Gibberellin Natural products 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 claims description 2
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 2
- 239000003448 gibberellin Substances 0.000 claims description 2
- 230000035755 proliferation Effects 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 abstract description 7
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 230000001902 propagating effect Effects 0.000 abstract description 2
- 241001584859 Colocasia <moth> Species 0.000 abstract 1
- 230000002062 proliferating effect Effects 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 240000004270 Colocasia esculenta var. antiquorum Species 0.000 description 12
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000037039 plant physiology Effects 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a rapid bulb propagation method for betel nut taro seeds, belonging to the technical field of rapid propagation of plant seedlings. The specific method comprises applying 0.1% HgCl to the stem tip of the stem bud of colocasia esculenta2Surface sterilizing for 10-15 min, inoculating to inducing culture medium, culturing in test tube seedling after 50-60 days, transferring test tube seedling to proliferating culture medium, propagating in large amount, cutting off bud, transferring to MS solid hormone-free culture medium, extending bud to 8-15 cm after 20-25 days, putting test tube seedling in liquid or solid culture medium, and expanding test tube seedling stem to form test tube bulb with weight of 0.2-10 g after 50-60 days. The method comprisesFast reproduction speed, high reproduction rate, direct and simple transplantation of test tube corms to field, labor and cost saving, etc.
Description
The invention belongs to the quick propagating technology field of plant seedling, be exclusively used in of the quick breeding of yam betel kind with bulb.
Bulb is divided into stalwart taro with taro, three types of how sub-taro and bull taros.Manual information retrieval is to domestic two pieces of research reports.Yang Naibo, the tissue culture of 1994. taros. the test-tube plantlet of report is induced with proliferated culture medium and is respectively MS+NAA 0.2mg/l+6-BA 2.0mg/l and MS+NAA2.0mg/l+6-BA 0.2mg/l agar solid culture medium in the Plant Physiology Communications 30 (5) 357.Xu Pei literary composition 1991, the tissue culture of taro, used inducing culture is a B5+BA 3.0mg/l+NAA 0.1mg/l agar solid culture medium in the Plant Physiology Communications 27 (4) 295; Plant behind the rooting of vitro seedling in the vermiculite, water B5 macroelement and liquid microelement every day, seedling survives after two weeks.According to the books light disk retrieval result of Agricultural University Of Nanjing, taro stem apex cultured in vitro adds 10 again at LS+NAA 5.5mg/l+KT 0.2mg/l (LS1) or LS+1.85mg/l+KT2mg/l (LS2)
-3--4Directly form test-tube plantlet on the medium of mol/l polyamines.Report such as Y am am o to (1992) Colocassiaescu len ta cv.Ish ikaw a-w ase test-tube plantlet forms the cormel in vitro of 1 grammes per square metre after last 40 day at the liquid nutrient medium of MS+ sucrose 8%.More than why type taro of all undeclared used material in all research reports, test-tube seedling transplanting needs intermediate link to the land for growing field crops, test-tube plantlet to be planted in the vermiculite, water nutrient solution every day, all these processes all need certain man power and material's investment, have improved the production cost of planting with bulb.
Yam betel belongs to stalwart taro type, and bulb has very high nutritive value and health care.Area, the Yangtze river basin is being carried out large tracts of land and is being introduced a fine variety the popularization experiment in cultivation in recent years.But because this crop field natural propagation coefficient low (a year reproduction coefficient is 3-5) can not be produced enough kinds at short notice and satisfy the production demand with bulb.The inventor quotes forefathers and induces propagation and cormel in vitro to induce result in the research at the taro test-tube plantlet, and test of many times is achieving success all.Therefore, the yam betel kind remains a problem that presses for solution with the quick breeding of bulb.
The purpose of this invention is to provide a kind of yam betel kind bulb method for quickly breeding, induce the pointed one-tenth test-tube plantlet of yam betel plants stems, find inducing and breeding prescription of yam betel test-tube plantlet, reproduction coefficient is reached more than 15.The test-tube plantlet of inducing generation directly forms bulb in culture vessel be cormel in vitro, and cormel in vitro can directly be cultivated the field, saves labor, and reduces production costs.
Yam betel kind bulb method for quickly breeding provided by the present invention, the concrete operations step is as follows:
1, with the stem apex 0.1%HgCl of yam betel bulb bud
2Surface sterilizing 10-15 minute, behind the aseptic water washing, be inoculated into prescription and be basal culture medium+1-3 times of FeSO of MS solid-based
4+ 6-BA (6-benzyl aminopurine) 1.0mg/l (culture volume, Hereinafter the same)+NAA (α-Nai Yisuan) 0.1mg/l+GA
3(gibberellin) 0.05mg/l, on the inducing culture of pH 5.8, after 50-60 days, the stem apex of bud develops into test-tube plantlet;
2, test-tube plantlet is changed over to the basic solid culture medium of MS+6-BA 0.5-1.5mg/l+IBA (IBA: indolebutyric acid) on the proliferated culture medium of 0.05-1.5mg/l (pH5.8), after 25-30 days, each test-tube plantlet base portion differentiation and proliferation, bud is downcut, repeat same enrichment culture process, breed test-tube plantlet in large quantities;
3, the bud that forms on the test-tube plantlet is downcut, changing the MS solid over to does not have in the hormone culture-medium, and after 20-25 days, bud is elongated to 8-15 centimetre;
4, above-mentioned test-tube plantlet is put into prescription for MS+ sucrose 8-12% (weight ratio, Hereinafter the same)+6-BA 4.0-10.0mg/l, pH4-6.0, the degree of depth is 1.0-2.0 centimetre a liquid nutrient medium, after 50-60 days, the base portion stem of test-tube plantlet expands, and forming weight is the cormel in vitro of 0.4-1.0 gram;
Perhaps wipe out the foundation portion of above-mentioned test-tube plantlet, insert MS+NAA 4.5-6.0mg/l+6-BA 1.0-3.0mg/l+ sucrose 8-12%, pH is in the 4.-6.0 solid culture medium, and after 50-60 days, the test-tube plantlet basal part of stem expands and forms the cormel in vitro that weight is the 0.2-0.6 gram.
The condition of above-mentioned culturing room is cultivated with normal conventional, adds 1-3 times of FeSO in the inducing culture
4Refer to intrinsic FeSO in the MS culture medium prescription
4The 1-3 of amount doubly.MS solid-based basal culture medium refers to MS macroelement+trace element+organic principle+0.6% agar+3% sucrose according to conventional usage.
After gathering, the above cormel in vitro of field cultivation fresh weight 0.2 gram of cormel in vitro directly is seeded in 1-2 centimetre of degree of depth in the soil.If weather and soil drought water when planting.Watered once every three days later on.After 10-15 days, terminal bud stretches out native face, and underground part forms complete root system, and management is with conventional field practice.As cloudy weather for several days running weather, can water.
The present invention compared with prior art has following advantage:
(1) compare with the method for field breeding yam betel kind with bulb, reproduction speed is fast, the reproduction rate height.A strain test-tube plantlet can be bred the 15-25 strain at 25-30 days among the present invention, and it is high to 10-15 centimetre to grow up through 20-25 days, can form through 50-60 days to plant again and use bulb.Every strain test-tube plantlet needs cormel in vitro of formation in 65-85 days.The test-tube plantlet breeding is calculated by per 30 days generation, annual 365 days, can breed test-tube plantlet 9-10 generation in preceding 300-260 days; The reproduction coefficient in per generation calculates by 15, and every strain test-tube plantlet can breed 15
9--10Strain.Be that every strain test-tube plantlet can be bred cormel in vitro 159--10 every year.(2) compare with the taro class crop method for tissue culture of having reported, the saving of labor economizes this, the survival rate height.The present invention adopts liquid and solid culture method directly to induce test-tube plantlet to form cormel in vitro.Adopt the rooting of vitro seedling method for transplanting in forefathers' the report, the domestication of test-tube plantlet requires certain temperature, and humidity and illumination condition need specific facility.Cormel in vitro directly is planted in the soil in field among the present invention, need not to protect facility, has reduced production cost.Cormel in vitro field cultivation survival rate is 100%, need not special operation simultaneously, guarantees when this method is used simple and reliable.
Embodiment 1
1, test-tube plantlet is induced: cut the tip of the bud that grows on the yam betel bulb, peel outside scale leaf off with scalpel, put into triangular flask, add 0.1%HgCl
2, kept 5-10 minute.On superclean bench, pour out HgCl
2After, add sterile water, change clothes 3-4 time.With material suck dry moisture on aseptic filter paper of surface sterilizing, be cut into 0.3-0.5 centimetre
2Piece of tissue, put on the inducing culture, cultivate in culturing room.Culturing room's condition is with common cultivation.The inducing culture based formulas is the FeSO of MS solid-based basal culture medium+1 times
4+ 6-BA 1.0mg/l+NAA 0.1mg/l+GA
30.05mg/l.After 50 days, the stem apex mounted blade grows up to test-tube plantlet.The piece of tissue of test-tube plantlet inductivity G formula Guan Miao/inoculation) be 55-60%.
2, the fast breeding of test-tube plantlet: test-tube plantlet is changed on the proliferated culture medium of the basic solid culture medium of MS+6-BA 1.5mg/l+IBA 0.5mg/l.After 25 days, each test-tube plantlet base portion is told the 20-25 strain.Again each bud is downcut, change in the same medium, repeat same process, a large amount of breeding test-tube plantlets.Ropagation coefficient of plantlet (bud is grown the test-tube plantlet number of body propagation outward in one month) is 20-25.
3, test-tube plantlet growth: the bud that forms on the test-tube plantlet is downcut, and changing the MS solid over to does not have in the hormone culture-medium, and after 20-25 days, bud is elongated to 8-15 centimetre.
4, inducing of cormel in vitro: above-mentioned high 8-15 centimetre test-tube plantlet is directly put into liquid nutrient medium.Culture medium prescription is MS+ sucrose 12%+6-BA 10.0mg/l; The medium degree of depth is 1.0-2.0 centimetre.After test-tube plantlet is put into, leave standstill and be placed in the culturing room.After 50-60 days, the base portion stem of test-tube plantlet expands, and forming weight is the cormel in vitro of 0.4-1.0 gram.Bulb is induced percentage G formula pipe bulb fresh weight greater than 0.2 gram/test-tube plantlet number) be 75-80%.
Embodiment 2
1, test-tube plantlet is induced: cut the tip of the bud that grows on the yam betel bulb, peel outside scale leaf off with scalpel, put into triangular flask, add 0.1%HgCl
2, kept 5-10 minute.On superclean bench, pour out HgCl
2After, add sterile water, change clothes 3-4 time.With material suck dry moisture on aseptic filter paper of surface sterilizing, be cut into 0.3-0.5 centimetre
2Piece of tissue, put on the inducing culture, cultivate in culturing room.Culturing room's condition is with common cultivation.The inducing culture based formulas is the FeSO of MS solid-based basal culture medium+3 times
4+ 6-BA 1.0mg/lNAA 0.1mg/l+GA
30.05mg/l.After 50 days, the stem apex mounted blade grows up to test-tube plantlet.Test-tube plantlet inductivity (piece of tissue of test-tube plantlet/inoculation) is 60-69%.
2, the fast breeding of test-tube plantlet: test-tube plantlet is changed on the proliferated culture medium of the basic solid culture medium of MS+6-BA 1.0mg/l+IBA 1.0mg/l.After 25 days, each test-tube plantlet base portion is told the 20-25 strain.Again each bud is downcut, change in the same medium, repeat same process, a large amount of breeding test-tube plantlets.Ropagation coefficient of plantlet (bud is grown the test-tube plantlet number of body propagation outward in one month) is 20-25.
3, test-tube plantlet growth: the bud that forms on the test-tube plantlet is downcut, and changing the MS solid over to does not have in the hormone culture-medium, and after 20-25 days, bud is elongated to 8-15 centimetre.
4, inducing of cormel in vitro: the foundation portion of wiping out above-mentioned test-tube plantlet, insert MS+NAA 6.0mg/l+6-BA 1.0mg/l+ sucrose 8%, pH is in the 4.-6.0 solid culture medium, and after 50-60 days, the test-tube plantlet basal part of stem expands and forms the cormel in vitro that weight is the 0.2-0.6 gram.It is 75-80% that bulb is induced percentage (the cormel in vitro fresh weight is greater than 0.2 gram/test-tube plantlet number).
Embodiment 3
Repeat embodiment 1, embodiment 2 operating process, test-tube plantlet inducing culture based formulas is MS+6-BA 1.0mg/l+NAA 0.1mg/l+GA
30.05mg/l, pH5.81.5 times of FeSO
4Medium in, the test-tube plantlet inductivity is 55-60%; 3 times of FeSO
4Medium in, inductivity is 60-69%; 2.5 FeSO doubly
4Medium in, inductivity is 50-68%.
Test-tube plantlet changes in the proliferated culture medium, and after 25-30 days, each test-tube plantlet grows the 15-25 strain.Proliferated culture medium is: MS+ agar+following composition, and pH5.86-BA 0.5mg/l+IBA 0.05mg/l, reproduction coefficient are 21-26; 6-BA 0.5mg/l+IBA 1.5mg/l, reproduction coefficient are 18-23; 6-BA 1.5mg/l+IBA 0.05mg/l, reproduction coefficient are 15-20; 6-BA 1.5mg/l+IBA 0.3mg/l, reproduction coefficient are 20-25; 6-BA 1.0mg/l+IBA 0.1mg/l, reproduction coefficient 15-256-BA 0.5mg/l+IBA 1.0mg/l, reproduction coefficient is 15-20.
The liquid shallow culture medium of inducing of cormel in vitro is the following composition 6-BA of a MS+ 4.0mg/l+ sucrose 8%, bulb inductivity 85-90%, fresh weight 0.4-0.8 gram 6-BA 4.0mg/l+ sucrose 12%, bulb inductivity 80-90%, fresh weight 0.5-0.8 gram 6-BA 10.0mg/l+ sucrose 8%, bulb inductivity 75-85%, fresh weight 0.4-0.8 gram 6-BA 10.0mg/l+ sucrose 10%, bulb inductivity 75-80%, fresh weight 0.4-1.0 gram 6-BA 5.0mg/l+ sucrose 10%, bulb inductivity 98-100%, fresh weight 0.5-1.0 gram
The solid inducing culture of cormel in vitro is MS+0.6% agar+following composition NAA 4.5+6-BA 1.0+ sucrose 8%, bulb inductivity 80-85%, bulb fresh weight 0.2-0.4 gram NAA 5.0+6-BA 1.0+ sucrose 10%, bulb inductivity 80-94%, bulb fresh weight 0.2-0.6 gram NAA 6.0+6-BA 1.0+ sucrose 10%, bulb inductivity 71-80%, bulb fresh weight 0.2-0.4 gram NAA 6.0+6-BA 3.0+ sucrose 10%, bulb inductivity 85-90%, bulb fresh weight 0.2-0.3 gram NAA 6.0+6-BA 3.0+ sucrose 12%, bulb inductivity 80-95%, bulb fresh weight 0.2-0.3 gram NAA 6.0+6-BA 3.0+ sucrose 12%, bulb inductivity 88-95%, bulb fresh weight 0.2-0.3 restrains (NAA and 6-BA are mg/l)
Claims (2)
1, a kind of yam betel kind bulb method for quickly breeding is characterized in that:
1) with the stem apex 0.1%HgCl of yam betel bulb bud
2Surface sterilizing 10-15 minute, behind the aseptic water washing, be inoculated into prescription and be basal culture medium+1-3 times of FeSO of MS solid-based
4+ 6-BA (6-benzyl aminopurine) 1.0mg/l (culture volume, Hereinafter the same)+NAA (α-Nai Yisuan) 0.1mg/l+GA
3(gibberellin) 0.05mg/l, on the inducing culture of pH 5.8, after 50-60 days, the stem apex of bud develops into test-tube plantlet;
2) test-tube plantlet is changed over to the basic solid culture medium of MS+6-BA 0.5-1.5mg/l+IBA (IBA: indolebutyric acid) on the proliferated culture medium of 0.05-1.5mg/l, after 25-30 days, each test-tube plantlet base portion differentiation and proliferation downcuts bud, repeat same enrichment culture process, breed test-tube plantlet in large quantities;
3) bud that forms on the test-tube plantlet is downcut, changing the MS solid over to does not have in the hormone culture-medium, and after 20-25 days, bud is elongated to 8-15 centimetre;
4) above-mentioned test-tube plantlet is put into prescription and be MS+ sucrose 8-12% (weight ratio, Hereinafter the same)+and 6-BA 4.0-10.0mg/l, the degree of depth is 1.0-2.0 centimetre a liquid nutrient medium, after 50-60 days, the base portion stem of test-tube plantlet expands, and forming weight is the cormel in vitro of 0.4-1.0 gram;
Perhaps wipe out the foundation portion of above-mentioned test-tube plantlet, insert MS+NAA 4.5-6.0mg/l+6-BA 1.0-3.0mg/l+ sucrose 8-12%, pH is in the 4.-6.0 solid culture medium, and after 50-60 days, the test-tube plantlet basal part of stem expands and forms the cormel in vitro that weight is the 0.2-0.6 gram.
2, yam betel kind bulb method for quickly breeding according to claim 1, it is characterized in that: the liquid nutrient medium degree of depth of test-tube plantlet culture test tube bulb is 1-2 centimetre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97118966A CN1073344C (en) | 1997-10-07 | 1997-10-07 | Method for propagating corms for betel nut taro seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97118966A CN1073344C (en) | 1997-10-07 | 1997-10-07 | Method for propagating corms for betel nut taro seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1179882A true CN1179882A (en) | 1998-04-29 |
| CN1073344C CN1073344C (en) | 2001-10-24 |
Family
ID=5175092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97118966A Expired - Fee Related CN1073344C (en) | 1997-10-07 | 1997-10-07 | Method for propagating corms for betel nut taro seeds |
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| CN (1) | CN1073344C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
| CN103535277A (en) * | 2013-09-18 | 2014-01-29 | 安徽省农业科学院园艺研究所 | Method for efficiently producing clocasia escalenta Schott microsphere stems |
| CN107667807A (en) * | 2016-07-31 | 2018-02-09 | 陆超 | A kind of yam betel cultural method |
| CN110384044A (en) * | 2019-08-16 | 2019-10-29 | 江西农业大学 | The breeding method of one ganoid konjaku taro detoxification seedling stem |
| CN110741925A (en) * | 2019-11-01 | 2020-02-04 | 广西壮族自治区农业科学院 | Cross breeding method for taro |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02286019A (en) * | 1989-04-28 | 1990-11-26 | Mitsui Petrochem Ind Ltd | Multiplication of tuber of araceae plant |
-
1997
- 1997-10-07 CN CN97118966A patent/CN1073344C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
| CN103535277A (en) * | 2013-09-18 | 2014-01-29 | 安徽省农业科学院园艺研究所 | Method for efficiently producing clocasia escalenta Schott microsphere stems |
| CN107667807A (en) * | 2016-07-31 | 2018-02-09 | 陆超 | A kind of yam betel cultural method |
| CN110384044A (en) * | 2019-08-16 | 2019-10-29 | 江西农业大学 | The breeding method of one ganoid konjaku taro detoxification seedling stem |
| CN110741925A (en) * | 2019-11-01 | 2020-02-04 | 广西壮族自治区农业科学院 | Cross breeding method for taro |
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| Publication number | Publication date |
|---|---|
| CN1073344C (en) | 2001-10-24 |
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