CN1465221A

CN1465221A - Nutrient solution spray cuttage

Info

Publication number: CN1465221A
Application number: CNA021223343A
Authority: CN
Inventors: 葛路美
Original assignee: Individual
Current assignee: Individual
Priority date: 2002-06-07
Filing date: 2002-06-07
Publication date: 2004-01-07
Anticipated expiration: 2022-06-07
Also published as: CN1180671C

Abstract

The present invention relates to the field of plant reproduction and seedling propagation technology, and is characterized by using special-made nutrient liquor and spraying it on the foliage of cuttage on the culture medium so as to promote the cutting quick grow. Said invention is low in cost, and high in reproduction efficiency.

Description

Nutrient solution spray cuttage

Technical field

The present invention relates to plant propagation seedling growing process field, is a kind of under simple and easy condition, uses the nutrient solution of special preparation, sprays to the plant plugged ear blade face of cuttage on medium, promotes the new technology of breeding of the quick growth of seedling, is called for short nutrient solution spray cuttage.

Background technology

The plant propagation seedling growing process, traditional have seed seedling-raising, stem tuber to grow seedlings and method such as cuttage and seedling culture.Along with the continuous progress of modern science and technology, the eighties of last century later stage, all in the cellular tissure of research,, carry out vegetative advanced person's the new technology of growing seedlings both at home and abroad by the method for aseptic culture with plant, be commonly called as " clone " or " group training " traditionally.This technology no doubt has its advance, but must buy complete instrument and equipment, also must set up the higher sterile working laboratory of environmental requirement, so its expense must increase.The while trivial operations, the seedling cost of cultivation is higher relatively, and speed is slow, and the large-scale industrialized production requirement of incompatibility more can not generally be promoted under simple and easy condition.

Summary of the invention

The objective of the invention is: provide a kind of to the plant propagation field of growing seedlings and under simple and easy condition, to carry out vegetative new method for raising seedling fast.It is characterized in that root, stem, the leaf of plant are cut into segment, suitably handle then, cuttage is in culture matrix.Under field conditions (factors), also can in booth or thermostatic equipment, carry out foliar spray, promote that seedling grows fast with the special nutrient solution of preparing.The essence of this method is a kind of seedling method that combines with traditional cottage method according to the basic principle of " tissue culture ".Adopt the inventive method to carry out artificial breeding, simple, need not carry out numerous and diverse sterile working, thereby less investment, Yan Zhi nutrient solution is with low cost voluntarily, and growth of seedling speed is fast, so the production efficiency height.Be particularly suitable for seedling such as the extensive breeding flowers of batch production, fruits and vegetables, traditional Chinese medicine.Also the making that can cultivate flowers and middle-size and small-size potted landscape in family's balcony, garden, office is beautified the environment.

Embodiment

Nutrient solution spray cuttage is applicable to the epicormic branch cuttage of most of plants.Specific operation process is as follows:

One, prepare the employing of plugged ear plugged ear and give birth to substantial then and not lignified half ripe branch, each plugged ear is got 2-3 joint, and the excision lower blade keeps top 2-3 sheet, and the less branch in blade face will suitably stay more, is beneficial to photosynthesis.Plugged ear lower end incision site should be under joint 0.5 centimeters, plugged ear should keep fresh and alive with inserting with adopting as far as possible.

Two, cutting medium matrix should meet the following requirements:

1, can support plugged ear does not tilt at certain angle;

2, can make the plugged ear base portion contact nutrient solution morely;

3, can make plugged ear base portion exchange of air;

4, be convenient to take root and later transplanting behind the plugged ear root of hair.

The material that can make cutting medium is a lot, and as peat, grit, razor clam stone, rock wool etc., especially the tailing sponge block of sponge factory is more suitable for doing cutting medium.The container of splendid attire matrix can be with light plastics square box, size and highly random.

Three, disinfection and preliminary treatment are example with sponge block as matrix, should will do sponge and the sterilizing containers and the preliminary treatment of matrix before the cuttage.Sponge block cuts into plastics square box internal diameter generally big or small, and then cuts into 5 * 6cm ²The fritter of size but does not switch at the end, only switches to 4/5 just passablely, and the bottom is also connected together, and is convenient to manage from now on.To be deployed into certain density liquor potassic permanganate and pour in the plastics square box, put sponge block then into, and squeeze and remove air, soaking sterilization.Also available formalin or ultraviolet sterilization.After several hours, solution colour is thin out, and complete soln is gone, and extracts sponge block, be placed in the plastic casing, and with partial application again in the middle of every blockage sponge block, the degree of depth is 3/4 thickness that it is standby to form crack.(, also must pass through above-mentioned preliminary treatment) as making matrix with other materials

Plugged ear also must be passed through preliminary treatment before cuttage, method is that plugged ear is immersed in 10～100ppmNAA solution 1～10 hour after with sterilization again in order to root of hair.

Four, cuttage is placed on plugged ear in the crack of above-mentioned sponge block, does not allow plugged ear tilt.The plugged ear vacuum side of blade is not attached on the sponge, in order to the air exchange of vacuum side of blade yet.After cuttage is finished, do not move or move back and forth plugged ear more arbitrarily.

Five, irritating liquid is to make by the plugged ear otch upwards to carry nutrient to arrive blade with the spraying purpose, generates organic matter through photosynthesis and is transported to each growing point, especially arrives cutting part and forms callus, tells rootlet again, makes it form independently plant fast.When irritating liquid, the nutrient solution for preparing is poured in the plastics square box, allows sponge block 1/3rd be immersed in the nutrient solution.Should not be advisable with moistening not dripping off and on to plugged ear foliage spray nutrient solution at ordinary times, spray again after waiting to do next time.With the various nutriments of continuous supply.

Six, the management of inserting after the management cuttage of back is very important, with good conditionsily preferably carries out thermostatic control.When growing seedlings on open country or the balcony, note daily temperature report, the minimum air temperature surpasses 20 ℃ just can carry out cuttage, and the maximum air temperature must not surpass 35 ℃, is also noted that when the sun is fierce and shades.

Embodiment botanical name: youth-and-old-age (herbage flower)

Get the branch top and be about 1.5 centimetres of plugged ears, because of its blade face less, for guaranteeing photosynthetic needs, so need not remove blade substantially.Cuttage instrument and matrix sponge block are carried out soaking disinfection, sterilization processing with 0.2% liquor potassic permanganate.Approximately till treating that potassium permanganate faded in 2 hours.With the light partial application of scraper, its degree of depth is advisable with 3/4 thickness of sponge on sponge.Squeeze the potassium permanganate that goes in the sponge, pour the nutrient solution that has prepared simultaneously into, allow it suck in the sponge.Slightly outwards exerting oneself in both sides with left index finger and middle finger flicking sponge knife-edge part, forms an osculum.At this moment refer to that with the right hand two the folder plugged ears are placed in the osculum in the sponge, and make it that low-angle be arranged slightly.Decontrol left hand two and refer to, utilize the elastic force of sponge to clamp plugged ear and just can.20 ℃～30 ℃ temperature, under certain illumination condition, and on the plugged ear blade face, sprayed the nutrient solution that has prepared every 1～4 hour, so that the blade face is moistening for spending, under just seeing sponge by the 3rd day the Bai Genliao of several length more than 1cm arranged,, can transplant again through in a few days.

Claims

1, a kind of vegetative propagation technique of breeding plant seedlings with nutrient solution spray cuttage fast.It is characterized in that according to the basic principle of " tissue culture " and the mode that traditional cuttage and seedling culture combines, plugged ear foliage spray nutrient solution on the culture matrix of the nutrient solution by special preparation is arranged in from filling to cuttage promotes plugged ear to develop into breeding and seedling method and the process of seedling plant fast.

2, nutrient solution spray cuttage according to claim 1 is characterized in that the matrix employing of medium can be supported the difficult inclination of plugged ear, and ventilative, soft material is as peat, grit, razor clam stone, rock wool, sponge block etc.

3, the culture matrix of nutrient solution spray cuttage according to claim 1 and plugged ear, sterilization and preliminary treatment must carry out disinfection before the cuttage.Sterilization method has potassium permanganate, dipped into formalin or ultraviolet irradiation, and preliminary treatment is meant suitably to be handled the requirement that meets cuttage with matrix and be placed in the suitable containers.The preliminary treatment of plugged ear is exactly that the plugged ear after the sterilization was immersed in 10～100ppmNAA solution 1～10 hour.

4, nutrient solution spray cuttage according to claim 1 is characterized in that irritating in the culture matrix nutrient solution that special preparation is arranged, at ordinary times should be often off and on to plugged ear foliage spray nutrient solution.

5, nutrient solution spray cuttage according to claim 1 is characterized in that the nutrient solution of medium and spraying usefulness, by formulated specially (seeing Table 1) voluntarily.

Table 1 nutrient solution preparation table Nomenclature of drug Chemical formula (code name) Consumption (mg/litre) Ammonium nitrate NH ₄NO ₃ ????1650 Potassium nitrate KNO ₃ ????190 Magnesium sulfate MgSO ₄.7H ₂O ????37 Calcium chloride Cacl ₂.2H ₂O ????40 Potassium dihydrogen phosphate KH ₂PO ₄ ????17 The EDTA disodium Na ₂.EDTA ????3.73 Ferrous sulfate FeSO ₄.7H ₂O ????2.78 Potassium iodide K1 ????0.083 Boric acid H ₃BO ₃ ????0.62 Manganese sulphate MnSO ₄.4H ₂O ????2.23 Zinc sulphate ZnSO ₄.7H ₂O ????0.86 Sodium molybdate Na ₂M ₀O ₄.2H ₂O ????0.025 Copper sulphate CuSO ₄.5H ₂O ????0.0025 Cobalt chloride COCl ₂.5H ₂O ????0.0025 Inositol C ₆H ₁₂O ₆ ????10 Nicotinic acid C ₆H ₅NO ₂ ????0.05 The hydrochloric acid pyridoxic acid CBH ₁₂ClNO ₃ ????0.05 Thiamine hydrochloride C ₁₂H ₁₇ClN ₄OS.HCl ????0.01 Glycine C ₂H ₅NO ₂ ????0.2 The basic element of cell division IAA 10ppm～100ppm inspires root Auximone NAA 10ppm～100ppm growth promotion Delay inhibitor B9 10ppm～100ppm prevents spindling