CN104541857A

CN104541857A - Method for cuttage seedling breeding in Myrica rubra Dongkui nutrition bowl

Info

Publication number: CN104541857A
Application number: CN201410788684.6A
Authority: CN
Inventors: 戚行江; 任海英; 梁森苗; 郑锡良; 颜丽菊
Original assignee: Zhejiang Academy of Agricultural Sciences
Current assignee: Zhejiang Academy of Agricultural Sciences
Priority date: 2014-12-17
Filing date: 2014-12-17
Publication date: 2015-04-29
Anticipated expiration: 2034-12-17
Also published as: CN104541857B

Abstract

The invention relates to a method for cuttage seedling breeding in a Myrica rubra Dongkui nutrition bowl. A cuttage strip base part is soaked in silver nitrate solution for half an hour; the cuttage strip base part is treated by indolacetic acid and the like; a mixture of peat and perlite serves as a substrate; cuttage seedling breeding is performed in a nutrition bowl. According to the seedling breeding method, the seedling breeding period is short (the whole seedling breeding period lasts for 9 months); the seedling breeding quality is high; the transplantation survival rate can be up to 100 percent and is obviously higher than that of seedbed seedling breeding; the method is convenient to operate and high in efficiency.

Description

A kind of Myrica rubra Dongkui nutritive cube cuttage and seedling culture method

(1) technical field

The present invention relates to a kind of Myrica rubra Dongkui nutritive cube cuttage and seedling culture method.

(2) background technology

Red bayberry is the distinctive fruit of south China, and domestic and international market has competitiveness, and Zhejiang is that red bayberry produces and the large province of scientific research, red bayberry area under cultivation has 1,300,000 mu, whole nation area under cultivation 5,000,000 mu, swift and violent in southwest growth momentum at present, need a large amount of healthy red bayberry nursery stocks.Breeding of current red bayberry nursery stock is main based on graft seedling growth.Propagation by grafiting advantage to keep fruit tree improved seeds characteristic, but the fruit tree virgin phase is longer, and reproduction speed is comparatively slow, and manual labor cost is higher.Cutting propagation is also important seedling-cultivating method on fruit tree, and especially in the upper use of grape (Yang Hongshuan, 2008) extensively, survival rate is more than 90%.The processing method of cuttage material and the influence research of environmental condition to adventive root more (Wang little Rong etc., 2003).Myrica(Myrica) cuttage root-taking difficulty seeds, cuttage survival rate is low, red bayberry cuttage and seedling culture have a small amount of report (king comes greatly, 1999; Li Minghong etc., 2008), but be all the bearing-age tree annual branch that grows directly from seeds that make use of life in 15 ~ 30 years, for current main breed Myrica rubra Dongkui also without any report.

East chief is the kind that in current red bayberry industry, area under cultivation is the widest, commodity property is best, but Myrica rubra Dongkui growth potential is strong, especially in first operation period often because growth potential is strong, go into operation late, early yield is low, of poor benefits.Cuttage and seedling culture main root is undeveloped, based on fibrous root, can weaken the growth potential of Myrica rubra Dongkui, therefore, in order to enable Myrica rubra Dongkui do sth. in advance result, in order to shorten growing-seedling period, reduce labour costs, be necessary the cuttage and seedling culture Improvement to Myrica rubra Dongkui, to obtaining efficient seedling-cultivating method, cultivate healthy nursery stock, shortening the juvenile phase, early go into operation, early income.

(3) summary of the invention

The object of the invention had both been to provide a kind of efficient, nursery stage is short, Quality of Seedlings is high Myrica rubra Dongkui nutritive cube cuttage and seedling culture method.

The technical solution used in the present invention is:

A kind of Myrica rubra Dongkui nutritive cube cuttage and seedling culture method, described method comprises:

(1) cutting prepares: select life in 15 years healthy Myrica rubra Dongkui tree annual branch, get its taper 8 ~ 12cm, stay 3 ~ 4 leaves, the truncation of fringe bar upper cut, lower cut are cut sth. askew, as cutting; Generally carry out in annual mid-June;

(2) cutting pretreatment: cutting base portion 2 ~ 3cm first uses the silver nitrate solution of 0.1 ~ 0.2% (w/w) to soak 5 ~ 6 hours, then soaks 20 ~ 30min with pretreatment fluid; Described pretreatment fluid is composed as follows: heteroauxin 300 ~ 400mg/kg, first dissolves heteroauxin by ethanol in proper amount, then is settled to desired concn with water;

(3) cuttage: the matrix that in nutritive cube, splendid attire is mixed by peat and perlite, inserts cutting;

(4) maintenance: nutritive cube is placed on seedling-cultivating tray, hide with plastics Small plastic shed, every day carefully sprays clear water with watering pot, and in maintenance Small plastic shed, relative moisture is more than 80%, natural lighting, until nursery completes, mid-March next year transplants on soil, gentle slope.

Preferably, described matrix is peat: the mixture of perlite mass ratio 5:1.

Preferably, described pretreatment fluid is composed as follows: heteroauxin 400mg/kg, first dissolves heteroauxin by ethanol in proper amount, then is settled to desired concn with water.

Of the present inventionly effectively to be mainly reflected in: the inventive method nursery stage short (whole nursery stage needs 9 months), Quality of Seedlings are high, adopt seedling-cultivating method of the present invention, transplanting survival rate can reach 100%, be significantly higher than seedbed nursery, and easy to operate, efficiency is high.

(4) embodiment

Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this:

Embodiment 1:

1 materials and methods

1.1 cuttings prepare

Select the annual branch of the healthy Myrica rubra Dongkui tree crown bottom of life in 15 years, get its taper 8 ~ 12cm, stay 3 ~ 4 leaves, the truncation of fringe bar upper cut, from lower end bud 0.5cm, lower cut adopts the back side to cut sth. askew, and otch will smoothly can not break.

1.2 experimental design

Cutting base portion 2 ~ 3cm silver nitrate solution of 0.2% soaks 6 hours, then processes 30 minutes with heteroauxin or naphthalene acid solution (add or do not add sucrose and vitamin B1).Often kind of hormone solution soaks cutting 900, on average inserts respectively in duplicate in the nutritive cube filling matrix (peat: perlite=5:1) and in the seedbed of yellow soil.10 kinds of processing modes are in table 1.

Table 1

1.4 cottage method

Nutritive cube is fitly emitted on seedling-cultivating tray, waters sufficient water in seedling-cultivating tray and nursery, and cutting carefully inserts one at each nutritive cube after handling well, and the cutting density of yellow soil is also 5cm × 5cm.

1.5 nursery bed management methods

Seedling-cultivating tray and nursery use plastics Small plastic shed to hide.Every day carefully sprays with watering pot, and in maintenance Small plastic shed, relative moisture is more than 80%.

1.6 nursery stock transplanting

After the Survival rate of nursery stock of nutritive cube and nursery lot, mid-March next year transplants to gentle slope acid soil ground, investigates plant percent, transplanting survival rate=(transplant survival seedling number/transplanted seedling number) × 100% to end of the year next year.

1.7 data survey

After cuttage, 5 months investigation Survival rate of nursery stock strain numbers, are calculated to be motility rate, cuttage survival rate=(surviving strain number/total strain number) × 100%.The representative plant that Stochastic choice 10 survives from often process, measure most long shoot tip length and the rugosity of new pumping, statistic diameters is greater than the branch number of 2cm, measures length and the rugosity of the longest root of new pumping, statistic diameters is greater than the number of the root of 2cm, averages.DPS software duncan's new multiple range method analyzes significant difference.

2 results and analysis

The red bayberry cutting of water treatment does not survive, the cutting that 4 kinds of hormone solution soak, and use nutritive cube substrate seedling to be generally better than the seedbed cuttage of yellow soil, wherein process 3 and 4 cutting survival rates the highest, be 54.4-57.8%, the two does not have significant difference.Process 1, process 2 and process 5 cutting survival rates are 30.4-45.8%.Cutting survival rate in yellow soil seedbed is all below 18%.

The newborn branch length of nutrient matrix feeding block seedlings raising and rugosity are 6-9.8cm and 1.8-2.1cm.Except processing 5, to survive the newborn branch length of seedling be 6.3cm in yellow soil seedbed, and other are all at below 5cm, and rugosity is between 1.4-1.6cm.Two kinds the different seedling raising manners tip is long and the thick most difference of the tip is not remarkable.Newborn tip quantity is substantially identical.

Nutrient matrix nursery survives shoot root length between 8.8-9cm, and yellow soil seedbed is long for 9.2cm except processing 5, and other are 4.2-5.8cm.It is 3 that nutrient matrix nursery survives shoot root number process 2 less, and other 3 are treated to 5-6 root, and process 5 radical of yellow soil is 4, but other process all only have 2.The rugosity of root, the root of process 8 is slightly 0.7cm, the thick 1-1.5cm of other all process roots, and difference is not remarkable.

The next year transplanting survival rate adopting feeding block seedlings raising of the present invention is all 100%, and the survival rate of seedling nursery is at 76-85%, the two significant difference.

Table 2: the red bayberry seedling growth parameter(s) of 5 months after cuttage

Note: lowercase is 5% significance level, and letter is all mutually does not have significant difference.

3 discuss

Result of study of the present invention finds, the annual spray of Myrica rubra Dongkui with 0.2% silver nitrate solution soak 6 hours after, the vitamin B1 solution of the sucrose+1mg/L of 400mg/kg heteroauxin or 400mg/kg heteroauxin+2% is used to soak half an hour again, then cuttage is in peat: in the nutritive cube of perlite=5:1, and cutting survival rate is 54.4-57.8%.This is lower than the survival rate 75.85-93.33% of grape, and this may be because the tannin generation of grape is far less than red bayberry, takes root easy than red bayberry.From production cost and simple to operate on can directly select 400mg/kg heteroauxin soak half an hour.

Indolebutyric acid (200mg/L) processes red bayberry seedling cutting of growing directly from seeds, cuttage root-taking survival rate 70-80% (king comes greatly to wait 1999), higher than the present invention survival rate, possible cause is the silver nitrate immersion cutting time is 12 hours, time less than the present invention's length 6, hormone solution soak time is 2-4 hour, time less than the long 1.5-3.5 of the present invention, also may be caused by Myrica rubra Dongkui cutting characteristic and seedling red bayberry cutting property difference.Although cuttage survival rate of the present invention is lower slightly, operating efficiency is higher than the research that king comes greatly to wait, and the present invention can complete within a working day, but the method that king comes greatly is due to long soaking time, cannot complete within a working day.And indolebutyric acid is used for Myrica rubra Dongkui, under equal conditions, cuttage survival rate is only about 30%, and after visible the present invention adopts heteroauxin process, cuttage plant percent is apparently higher than indolebutyric acid.The present invention adopts nutritive cube to contain the method nursery of matrix, and transplanting survival rate is significantly higher than seedbed nursery, and easy to operate.

Claims

1. a Myrica rubra Dongkui nutritive cube cuttage and seedling culture method, described method comprises:

(1) cutting prepares: select life in 15 years healthy Myrica rubra Dongkui tree annual branch, get its taper 8 ~ 12cm, stay 3 ~ 4 leaves, the truncation of fringe bar upper cut, lower cut are cut sth. askew, as cutting;

(2) cutting pretreatment: cutting base portion 2 ~ 3cm first soaks 5 ~ 6 hours with the silver nitrate solution of 0.1 ~ 0.2%, then soaks 20 ~ 30min with pretreatment fluid; Described pretreatment fluid is composed as follows: heteroauxin 300 ~ 400mg/kg, first dissolves heteroauxin by ethanol in proper amount, then is settled to desired concn with water;

(4) maintenance: nutritive cube is placed on seedling-cultivating tray, hide with plastics Small plastic shed, every day carefully sprays clear water with watering pot, and in maintenance Small plastic shed, relative moisture is more than 80%, and natural lighting, until nursery completes.

2. the method for claim 1, is characterized in that described matrix is peat: the mixture of perlite mass ratio 5:1.

3. the method for claim 1, is characterized in that described pretreatment fluid is composed as follows: heteroauxin 400mg/kg, first dissolves heteroauxin by ethanol in proper amount, then be settled to desired concn with water.