CN113575151A

CN113575151A - Poplar cuttage stable rooting method

Info

Publication number: CN113575151A
Application number: CN202110854832.XA
Authority: CN
Inventors: 陈肃; 于越; 曲冠证; 刘智杰
Original assignee: Northeast Forestry University
Current assignee: Northeast Forestry University
Priority date: 2021-07-28
Filing date: 2021-07-28
Publication date: 2021-11-02
Anticipated expiration: 2041-07-28
Also published as: CN113575151B

Abstract

The invention discloses a stable rooting method for poplar by cutting, and belongs to the technical field of tree species propagation. The stable rooting method for poplar cuttage comprises the following steps: wrapping the top end of the explant with the leaf with a water absorbing material, circularly cutting the base part, soaking in water for culture, dripping auxin on the water absorbing material during culture to obtain the explant with adventitious roots, and then carrying out cuttage. The stable rooting method for cuttage effectively reduces the use concentration of exogenous auxin, avoids the phenomenon of bacterial breeding caused by the adoption of the traditional culture method, achieves the purposes of stably rooting the cuttage branches of the poplar and improving the transplanting survival rate, and provides a new working method for vegetative propagation of the poplar and quick rooting of cuttings.

Description

Poplar cuttage stable rooting method

Technical Field

The invention relates to the technical field of tree species propagation, in particular to a stable rooting method for poplar by cutting.

Background

The poplar has strong adaptability to the environment and wide distribution, and can grow well in both fertile land and barren mountain land. The poplar has high economic use value and environmental protection value, has straight trunk, fine material and fast growth, is one of important tree species of paper pulp raw material forests, and is also one of afforestation tree species of excellent water source conservation forests and main protection forest zones.

At present, poplar is mainly propagated by cutting seedlings, but the conventional cutting survival rate is low, and the problem that the poplar is easy to die after cutting exists, in the prior art, the cutting slips are mainly treated by rooting powder or a high-concentration auxin solution before cutting, but sufficient adventitious roots can not be grown before cutting, and the phenomena that the cutting slips are wasted and the cost is increased are easily caused. How to improve the rooting rate and the survival rate of cuttage or transplantation of poplar cuttage branches becomes a difficult problem to be solved urgently by technical personnel in the field.

Disclosure of Invention

The invention aims to provide a stable rooting method for poplar by cutting, which aims to solve the problems in the prior art, promotes stable rooting of explants by circularly cutting the bases of explants with leaves and dripping growth regulators at the top ends of the explants, and effectively improves the survival rate of the explants after cutting and transplanting.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides a stable rooting method for poplar cuttage, which comprises the following steps: wrapping the top end of the explant with a water absorbing material, cutting the base part circularly, soaking in water for culturing, and dripping auxin on the water absorbing material during culturing to obtain the explant with adventitious roots.

Further, the explant is a branch of a poplar; the poplar is selected from populus tremuloides, populus nigra, populus tremuloides, populus alba, populus diversifolia or populus euphratica; the length of the explant is 5-15 cm; the explant comprises at least 3 bud sites and 1 leaf.

Still further, the aspen is selected from aspen chinese, triploid populus alba or populus alba.

Further, the explant comprises 3-4 bud sites.

Further, the ring cutter specifically includes: performing circular cutting at a position 0.5-1.5 cm above the base of the explant; the width of the circular cut is 0.2-1 cm;

further, the ring-cutting depth is the sum of the thicknesses of the bark and the phloem.

Further, the water absorbent material includes at least one of absorbent gauze and absorbent cotton.

Further, the auxin comprises one or more of indolebutyric acid, naphthylacetic acid and indoleacetic acid.

Further, the concentration of the auxin is 0.1-0.5 mg/mL.

Further, the culturing specifically comprises: culturing for 10-25 days under the conditions that the temperature is 25-30 ℃, the illumination time is 16h and the illumination intensity is 2800-3000 Lux.

Further, the method also comprises the step of cutting the explant with the adventitious roots in mixed nutrient soil for culture after obtaining the explant with the adventitious roots.

Further, the mixed nutrient soil is nutrient soil: vermiculite: perlite is added into the mixture in a volume ratio of 5: 2: 1, uniformly mixing and then sterilizing; the cutting depth is 3-5 cm.

Furthermore, the nutrient soil is humus nutrient soil.

The invention discloses the following technical effects:

(1) the stable rooting method for the poplar cuttings effectively reduces the using concentration of the auxin, compared with the prior art, the using concentration of the auxin is reduced by tens of times to hundreds of times, the method is simple and easy to implement, the rooting period of the branches can be shortened, the method can be used for quickly and massively culturing the cuttage branches, and the preparation cost of the cuttage branches is saved.

(2) The invention adopts the method of dripping auxin on the water absorbing material at the top end of the explant, promotes the rooting of the explant and solves the problems of the breeding phenomenon of bacteria at the base of the explant and the difficulty in removing the bacteria caused by the adoption of the method of soaking the base in the prior art.

(3) The invention provides a method for stable rooting of poplar by cutting, which comprises the steps of wrapping a water absorbing material at the top end of an explant with leaves and bud points, circularly cutting the base part of the explant, immersing the explant in water for culture, and dripping auxin on the water absorbing material during the culture, thereby effectively reducing the time required by rooting of the explant, reducing the use concentration of the auxin, saving time and manpower, achieving the purpose of stable rooting of branches of the poplar, transplanting the rooted explant after cutting culture, improving the survival rate and the growth speed after transplanting, and providing a new working method for vegetative propagation of poplar and rapid rooting of cuttings.

(4) The leaves left by the explant can generate organic matters such as auxin, cane sugar and the like required by rooting, so that the cutting rooting is facilitated, and nutrient substances are provided for the rooting of the explant.

(5) The explant base girdling method adopted by the invention can enable nutrients generated by photosynthesis of the leaf parts to be accumulated above the girdling parts, and has a promoting effect on rooting of healing tissues.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a diagram of a seedling of Populus tremuloides cultivated in example 1 of the present invention;

FIG. 2 is a diagram of a seedling of Populus tremula obtained by the cultivation in step (4) of example 5 of the present invention;

FIG. 3 is a diagram of a triploid Populus tremula seedlings cultured in step (4) of example 6 of the present invention;

FIG. 4 is a diagram of Populus alba seedlings cultured in step (4) of example 7 of the present invention.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

Example 1

A stable rooting method for poplar cuttage comprises the following steps:

(1) preparing an explant: cutting robust branches from grown plant populus tremuloides, and cutting the branches into branches with the length of about 12cm and 2 leaves with 4 bud points as explants.

(2) Peeling off the epidermis at a position about 1cm above the explant base by adopting a circular cutting method, wherein the peeling width is about 0.5cm, the peeling thickness is the sum of the thicknesses of the explant bark and the phloem (on the premise of not damaging the xylem), obtaining the explant with the base subjected to circular cutting, then winding absorbent cotton at the top end of the explant and fixing the explant by using a rubber band.

(3) Putting the explant obtained by the treatment in the step (2) into a plastic tissue culture bottle, adding clear water, wherein the water amount is higher than that of a cutting slip girdling part, then putting the explant into a greenhouse with the temperature of 26 +/-1 ℃, the illumination time of 16h and the illumination intensity of 2800Lux for rooting culture, dropwise adding 0.2mg/mL indolebutyric acid on absorbent cotton at the top end of the explant during the culture, dropwise adding 1mL indolebutyric acid every day, and culturing for 14 d.

(4) Inserting the explant with the adventitious roots prepared in the step (3) into sterilized mixed nutrient soil (the mixed nutrient soil is obtained by mixing nutrient soil, vermiculite and perlite according to the volume ratio of 5: 2: 1 and then sterilizing), wherein the insertion depth is about 4cm, and culturing for 18d to obtain young populus erecta seedlings (see figure 1).

Example 2

The same as example 1 except that the concentration of indolebutyric acid in step (3) is 0.1 mg/mL.

Example 3

The same as example 1, except that the concentration of indolebutyric acid in step (3) is 0.5 mg/mL.

Example 4

The same as example 1, except that the concentration of indolebutyric acid in step (3) is 1 mg/mL.

Example 5

The difference from the example 1 is that the explant in the step (1) is Chinese aspen branch; the growth of the seedlings cultured in the step (4) is shown in FIG. 2.

Example 6

The difference from example 1 is that the explants in step (1) are triploid poplar shoots; the growth of the seedlings cultured in the step (4) is shown in FIG. 3.

Example 7

The difference from example 1 is that the explants in step (1) are poplar shoots; the growth of the seedlings cultured in the step (4) is shown in FIG. 4.

Comparative example 1

(1) Preparing an explant: cutting robust branches from grown plant populus tremuloides, and cutting the branches into branches with the length of about 12cm and 4 bud points to serve as explants.

(2) Putting the explant obtained by the treatment in the step (1) into a plastic tissue culture bottle, adding indolebutyric acid with the concentration of 400mg/L to soak the cuttings, and then putting the explant into a greenhouse with the temperature of 26 +/-1 ℃, the illumination time of 16h and the illumination intensity of 2800Lux to perform rooting culture for 14 d.

And (4) counting the adventitious root length, the induction rate and the cuttage survival rate of the explants cultured in the step (3) of the examples 1-7 and the comparative example 1.

TABLE 1 explant adventitious root length

Examples	Adventitious root induction ratio (%)	Length of adventitious root (cm)	Survival rate of cuttage (%)
				Example 1	87.5	8.2	100
Example 2	60	4.25	100
				Example 3	47.5	3.85	100
Example 4	27.5	3.5	100
				Example 5	90	3.75	100
Example 6	85	4.2	100
				Example 7	70	1.8	100
Comparative example 1	45	3.25	67.5

Note: the induction rate calculation mode of the adventitious root is as follows: the number of rooting cuttings/total cuttings is multiplied by 100%;

the length of the adventitious root refers to: length of the adventitious root master root;

the cuttage survival rate calculation mode is as follows: the number of the development cuttings/total cutting cuttings after cutting is multiplied by 100 percent.

As can be seen from Table 1, the application of 0.2mg/L IBA resulted in the best rooting promotion effect on the cutting of Populus tremuloides.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. A stable rooting method for poplar cuttage is characterized by comprising the following steps: wrapping the top end of the explant with a water absorbing material, cutting the base part circularly, soaking in water for culturing, and dripping auxin on the water absorbing material during culturing to obtain the explant with adventitious roots.

2. The stable rooting by poplar cutting method according to claim 1, wherein the explant is a shoot of a poplar; the poplar is selected from populus tremuloides, populus nigra, populus tremuloides, populus alba, populus diversifolia or populus euphratica; the length of the explant is 7-15 cm; the explant comprises at least 3 bud sites and 1 leaf.

3. The poplar cutting stable rooting method according to claim 1, wherein the ring cutting specifically comprises: performing circular cutting at a position 0.5-1.5 cm above the base of the explant; the width of the circular cutting is 0.2-1 cm.

4. The poplar cutting stable rooting method according to claim 1, wherein the girdling depth is the sum of thicknesses of bark and phloem.

5. The poplar cutting stable rooting method according to claim 1, wherein the water absorbing material comprises at least one of absorbent gauze and absorbent cotton.

6. The poplar cutting stable rooting method according to claim 1, wherein the auxin comprises one or more of indolebutyric acid, naphthylacetic acid and indoleacetic acid.

7. The stable rooting method for poplar cuttage according to claim 6, wherein the concentration of the auxin is 0.1-0.5 mg/mL.

8. The poplar cutting stable rooting method according to claim 1, wherein the culturing specifically comprises: culturing for 10-25 days under the conditions that the temperature is 25-30 ℃, the illumination time is 16h and the illumination intensity is 2800-3000 Lux.

9. The stable rooting by cutting method for poplar as claimed in claim 1, wherein the method further comprises culturing the explant after obtaining adventitious roots by cutting in mixed nutrient soil.

10. The poplar cutting stable rooting method according to claim 9, wherein the mixed nutrient soil is a nutrient soil: vermiculite: perlite is added into the mixture in a volume ratio of 5: 2: 1, uniformly mixing and then sterilizing; the cutting depth is 3-5 cm.