CN102550382A - Rooting transplanting method for tissue culture poplar seedlings - Google Patents
Rooting transplanting method for tissue culture poplar seedlings Download PDFInfo
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Abstract
A rooting transplanting method for tissue culture poplar seedlings is used for solving the problem that the existing rooting transplanting methods lead to low survival rate of the tissue culture poplar seedlings. The rooting transplanting method for tissue culture poplar seedlings includes: firstly, placing tissue culture poplar seedlings into test tubes containing liquid rooting medium, erecting the tissue culture poplar seedlings, covering openings of the test tubes with seal films, allowing the seedlings to root to obtain the rooted seedlings; secondly, moving the rooted seedlings with 12-14mm long roots to a container with distilled water from the test tubes, and covering the rooted seedlings with a transparent container to preserve moisture for water culture; and thirdly, transplanting the rooted seedlings subjected to water culture to soil, covering the rooted seedlings with a transparent container to preserve moisture for 16 days, removing the transparent container, transplanting the rooted seedlings with the soil into a greenhouse and completing rooting and transplanting of the tissue culture poplar seedlings. The rooting transplanting method is safe and reliable, and the transplant survival rate of the tissue culture poplar seedlings transplanted into the greenhouse can reach more than 95%.
Description
Technical field
The present invention relates to a kind of rooting and transplant method of willow tissue cultivating seedling.
Background technology
Plant Tissue Breeding comprises the explant collection, a series of processes such as culture medium preparation, can, sterilization, deposit, inoculation, cultivation.Obtain need carry out rooting and transplant behind the tissue cultivating seedling, conventional method uses solid culture medium to take root, indoor refining seedling or soil refining seedling, the rooting and transplant method of direct transplanting booth afterwards.The willow tissue cultivating seedling is through after the culture of rootage, and direct transplanting booth refining seedling is prone to dehydration wilts, and causes the survival rate of tissue cultivating seedling low.After the willow tissue cultivating seedling process solid culture of rootage, clean the process of root medium and hinder root easily, reduced the water absorbing capacity of root.Conventional method need be carried out spraying and moisturizing to growing environment, and is higher to environmental requirement, and blade is very easy to dehydration and wilts, and causes the transplanting survival rate of tissue cultivating seedling low.
Summary of the invention
The present invention will solve the low problem of survival rate that existing rooting and transplant method makes the willow tissue cultivating seedling, and a kind of rooting and transplant method of willow tissue cultivating seedling is provided.
The rooting and transplant method of willow tissue cultivating seedling of the present invention, carry out according to the following steps: one, the willow tissue cultivating seedling is put into the test tube that 4～6mL liquid root media is housed, make tissue cultivating seedling upright, envelope bottle film was taken root the seedling of must taking root 11～13 days on the test tube flap; Two, the seedling of taking root that root is reached 12～14mm moves on to from test tube in the container that distilled water is housed, and the depth of water is 10～20mm, on the seedling of taking root, buckles transparent vessel and preserves moisture, water planting 2 days; Three, then with taking root transplantation of seedlings in soil after the water planting, on the seedling of taking root, buckle transparent vessel and preserved moisture 16 days, water 1 wherein preceding 4 day every day, and the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in afterwards 6 days per 2 days, the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in last 6 days per 3 days, and removed transparent vessel then, the seedling balled transplanting of taking root in the soil to booth, is promptly accomplished the rooting and transplant of willow tissue cultivating seedling; Wherein the said soil of step 3 is made up of by weight 6: 1 turfy soil and vermiculite.
Advantage of the present invention is following:
1, the inventive method is safe and reliable, be transplanted to booth after transplanting survival rate reach more than 95%.
2, utilize Boiling tube to take root, traditional existing rooting method will add agar in medium, and like this, the just necessary residual agar of flush away root is hindered root easily before transplanting, and transplanting survival rate is reduced.The present invention is taken root with Boiling tube, and the willow tissue cultivating seedling can be upright in test tube, takes root with the liquid root media, before transplanting, removed the step of washing agar from, avoided hindering root, improved transplanting survival rate.
3, owing to contain 2% sucrose in the root media, the osmotic pressure of medium is higher like this, is transplanted to soil, and afterwards, osmotic pressure acutely changes, and influences transplanting survival rate.The present invention cultivated in water 2 days at the back of taking root, and changed the osmotic pressure environment of root, made it approaching with the soil of transplanting, and made the root after the transplanting in soil, recover water absorbing capacity rapidly, increased transplanting survival rate.
4, the present invention detains transparent vessel and preserves moisture on the seedling of taking root, and not limited by indoor humidity, and has reduced the evaporation of moisture, has avoided the poplar leaf dehydration, has increased the survival rate of plant.
5, soil need not be sterilized: soil sterilization is the material circumstance of traditional group training transplantation of seedlings, transplants the soil sterilization difficulty on a large scale; Traditional rooting method is put into sucrose and agar in medium, in the process of flush away agar, it is residual to have agar; It is residual also to have sucrose; Increased the possibility of root contaminating microorganisms like this, and then produced mashed root, survival rate reduces.The inventive method is carried out the liquid program with water planting of taking root, and has reduced the residual of root sucrose and agar, and then has reduced the possibility of contaminating microorganisms, therefore, utilizes this method to take root, and transplanting soil need not be sterilized, and is convenient and practical.
Description of drawings
Fig. 1 is the willow tissue cultivating seedling photo that liquid is taken root in test tube in the embodiment seven; The take root seedling photo of Fig. 2 for obtaining after taking root 12 days in the embodiment seven; Fig. 3 is the photo of the willow tissue cultivating seedling that traditional rooting method was taken root 12 days in the embodiment seven; Fig. 4 is the seedling photo of taking root of water planting in the step 2 in the embodiment seven; Fig. 5 is the photo of transplantation of seedlings in the soil of taking root in the step 3 in the embodiment seven; Fig. 6 is for removing the seedling photo of taking root of transparent plastic cup after preserving moisture 16 days in rapid three in the embodiment seven.
Embodiment
Technical scheme of the present invention is not limited to following cited embodiment, also comprises the combination in any between each embodiment.
Embodiment one: the rooting and transplant method of this embodiment willow tissue cultivating seedling; Carry out according to the following steps:: one, the willow tissue cultivating seedling is put into the test tube that 4～6mL liquid root media is housed, make tissue cultivating seedling upright, envelope bottle film on the test tube flap; Took root the seedling of to take root 11～13 days; Two, the seedling of taking root that root is reached 12～14mm moves on to from test tube in the container that distilled water is housed, and the depth of water is 10～20mm, on the seedling of taking root, buckles transparent vessel and preserves moisture, water planting 2 days; Three, then with taking root transplantation of seedlings in soil after the water planting, on the seedling of taking root, buckle transparent vessel and preserved moisture 16 days, water 1 wherein preceding 4 day every day, and the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in afterwards 6 days per 2 days, the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in last 6 days per 3 days, and removed transparent vessel then, the seedling balled transplanting of taking root in the soil to booth, is promptly accomplished the rooting and transplant of willow tissue cultivating seedling; Wherein the said soil of step 3 is made up of by weight 6: 1 turfy soil and vermiculite.
Temperature remains on 25 ℃ in the rooting and transplant process of this embodiment, and illumination condition is 8 hours dark of illumination 16 hours every days.
Embodiment two: what this embodiment and embodiment one were different is: the tissue cultivating seedling of willow described in the step 1 has a terminal bud, 6～8 lateral lobes, plant heights are that 6～9cm and stem slightly are 0.6～1.5mm.Other is identical with embodiment one.
Embodiment three: what this embodiment was different with embodiment one or two is: the diameter of test tube described in the step 1 is 30mm, and length is 20cm.Other is identical with embodiment one or two.
Embodiment four: what this embodiment was different with one of embodiment one to three is: in the step 1 willow tissue cultivating seedling is put into the test tube that 5mL liquid root media is housed.Other is identical with one of embodiment one to three.
Embodiment five: what this embodiment was different with one of embodiment one to four is: took root in the step 1 12 days.Other is identical with one of embodiment one to four.
Embodiment six: what this embodiment was different with one of embodiment one to five is: the depth of water is 15mm in the step 2.Other is identical with one of embodiment one to five.
Embodiment seven: the rooting and transplant method of this embodiment willow tissue cultivating seedling; Carry out according to the following steps: the new poplar tissue cultivating seedling in mountain that, will have a terminal bud, 6～8 lateral lobes, plant heights and be 6～9cm and stem and slightly be 0.6～1.5mm is put into the test tube that 5mL liquid root media is housed; Make tissue cultivating seedling upright; Envelope bottle film was taken root the seedling of must taking root 12 days on the test tube flap; Two, the seedling of taking root that root is reached 12～14mm moves on to from test tube in the plastic cup that distilled water is housed, and the depth of water is 15mm, on the seedling of taking root, buckles the transparent plastic cup and preserves moisture, water planting 2 days; Three, then with taking root transplantation of seedlings in soil after the water planting, on the seedling of taking root, buckle transparent vessel and preserved moisture 16 days, water 1 wherein preceding 4 day every day, and the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in afterwards 6 days per 2 days, the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in last 6 days per 3 days, and removed transparent vessel then, the seedling balled transplanting of taking root in the soil to booth, is promptly accomplished the rooting and transplant of willow tissue cultivating seedling; Wherein the said soil of step 3 is made up of by weight 6: 1 turfy soil and vermiculite.
Temperature remains on 25 ℃ in the rooting and transplant process of this embodiment, and illumination condition is 8 hours dark of illumination 16 hours every days.
The diameter of test tube described in the step 1 is 30mm, and length is 20cm, sows rich garden supplies Co., Ltd by Shanghai and produces.
The root media of liquid described in the step 1 is for containing the MS medium of 0.25mg/L NAA (NAA), 5.5g agar and 20g sucrose.
The new poplar in mountain described in the step 1 is to be female parent with aspen (Populus.dividiana), is the artificial hybridization seeds of male parent with Xinjiang poplar (Populus.bolleana).
The transplanting survival rate of the new poplar tissue cultivating seedling in this embodiment mountain is 98%.
The new poplar tissue cultivating seedling photo that liquid is taken root in test tube in this embodiment mountain is as shown in Figure 1; The seedling photo of taking root that obtained after taking root 12 days is as shown in Figure 2; The photo of the willow tissue cultivating seedling that traditional rooting method was taken root 12 days is as shown in Figure 3; Can find out on the root of conventional method and adhere to medium, clean the back and just hinders root easily.The seedling photo of taking root of water planting is as shown in Figure 4 in the step 2, and the transplantation of seedlings of taking root in the step 3 is as shown in Figure 5 to the photo in the soil, and the seedling photo of taking root of removing the transparent plastic cup after preserving moisture in the step 3 16 days is as shown in Figure 6.
Embodiment eight: the rooting and transplant method of this embodiment willow tissue cultivating seedling; Carry out according to the following steps: the tissue cultivating seedling that, will have a terminal bud, 6～8 lateral lobes, plant heights and be 6～9cm and stem and slightly be the American-European poplar 108 of 0.6～1.5mm is put into the test tube that 5mL liquid root media is housed; Make tissue cultivating seedling upright; Envelope bottle film was taken root the seedling of must taking root 12 days on the test tube flap; Two, the seedling of taking root that root is reached 12～14mm moves on to from test tube in the plastic cup that distilled water is housed, and the depth of water is 15mm, on the seedling of taking root, buckles the transparent plastic cup and preserves moisture, water planting 2 days; Three, then with taking root transplantation of seedlings in soil after the water planting, on the seedling of taking root, buckle transparent vessel and preserved moisture 16 days, water 1 wherein preceding 4 day every day, and the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in afterwards 6 days per 2 days, the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in last 6 days per 3 days, and removed transparent vessel then, the seedling balled transplanting of taking root in the soil to booth, is promptly accomplished the rooting and transplant of willow tissue cultivating seedling; Wherein the said soil of step 3 is made up of by weight 6: 1 turfy soil and vermiculite.
Temperature remains on 25 ℃ in the rooting and transplant process of this embodiment, and illumination condition is 8 hours dark of illumination 16 hours every days.
The diameter of test tube described in the step 1 is 30mm, and length is 20cm, sows rich garden supplies Co., Ltd by Shanghai and produces.
The root media of liquid described in the step 1 is for containing the WPM medium of 0.4mg/L IBA (indolebutyric acid), 5.5g agar and 20g sucrose.
American-European poplar 108 described in the step 1 is the crossbreed of eastern cottonwood (Populus deltoids) and Populus nigra (Populus nigra).
The transplanting survival rate of the tissue cultivating seedling of the American-European poplar 108 of this embodiment is 97%.
Claims (6)
1. the rooting and transplant method of a willow tissue cultivating seedling; The rooting and transplant method that it is characterized in that the willow tissue cultivating seedling; Carry out according to the following steps: one, the willow tissue cultivating seedling is put into the test tube that 4～6mL liquid root media is housed, make tissue cultivating seedling upright, envelope bottle film on the test tube flap; Took root the seedling of to take root 11～13 days; Two, the seedling of taking root that root is reached 12～14mm moves on to from test tube in the container that distilled water is housed, and the depth of water is 10～20mm, on the seedling of taking root, buckles transparent vessel and preserves moisture, water planting 2 days; Three, then with taking root transplantation of seedlings in soil after the water planting, on the seedling of taking root, buckle transparent vessel and preserved moisture 16 days, water 1 wherein preceding 4 day every day, and the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in afterwards 6 days per 2 days, the back of at every turn watering uses running water in the spraying of transparent vessel inwall once; Watered 1 time in last 6 days per 3 days, and removed transparent vessel then, the seedling balled transplanting of taking root in the soil to booth, is promptly accomplished the rooting and transplant of willow tissue cultivating seedling; Wherein the said soil of step 3 is made up of by weight 6: 1 turfy soil and vermiculite.
2. the rooting and transplant method of a kind of willow tissue cultivating seedling according to claim 1 is characterized in that the tissue cultivating seedling of willow described in the step 1 has a terminal bud, 6～8 lateral lobes, plant heights are that 6～9cm and stem slightly are 0.6～1.5mm.
3. the rooting and transplant method of a kind of willow tissue cultivating seedling according to claim 1 and 2, the diameter that it is characterized in that test tube described in the step 1 is 30mm, length is 20cm.
4. the rooting and transplant method of a kind of willow tissue cultivating seedling according to claim 3 is characterized in that in the step 1 willow tissue cultivating seedling being put into the test tube that 5mL liquid root media is housed.
5. the rooting and transplant method of a kind of willow tissue cultivating seedling according to claim 4 is characterized in that taking root in the step 1 12 days.
6. the rooting and transplant method of a kind of willow tissue cultivating seedling according to claim 5 is characterized in that the depth of water is 15mm in the step 2.
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Cited By (6)
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| CN104335887A (en) * | 2014-10-14 | 2015-02-11 | 金陵科技学院 | Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid |
| CN105052715A (en) * | 2015-08-03 | 2015-11-18 | 山东省林业科学研究院 | Populus breeding device and use method thereof |
| CN108575359A (en) * | 2018-04-27 | 2018-09-28 | 北京农学院 | A kind of processing of Malus admiring fruit tree training tissue culture seedling and tissue-cultured seedling rooting method |
| CN108849509A (en) * | 2018-07-05 | 2018-11-23 | 内蒙古和盛生态育林有限公司 | A kind of cultural method of poplar microbiological contamination tissue-cultured seedling |
| CN112868529A (en) * | 2021-02-04 | 2021-06-01 | 中国林业科学研究院林业研究所 | Method for improving seedling survival rate of hybrid filial generation of populus nigra |
| CN113575151A (en) * | 2021-07-28 | 2021-11-02 | 东北林业大学 | Poplar cuttage stable rooting method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104335887A (en) * | 2014-10-14 | 2015-02-11 | 金陵科技学院 | Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid |
| CN105052715A (en) * | 2015-08-03 | 2015-11-18 | 山东省林业科学研究院 | Populus breeding device and use method thereof |
| CN108575359A (en) * | 2018-04-27 | 2018-09-28 | 北京农学院 | A kind of processing of Malus admiring fruit tree training tissue culture seedling and tissue-cultured seedling rooting method |
| CN108849509A (en) * | 2018-07-05 | 2018-11-23 | 内蒙古和盛生态育林有限公司 | A kind of cultural method of poplar microbiological contamination tissue-cultured seedling |
| CN112868529A (en) * | 2021-02-04 | 2021-06-01 | 中国林业科学研究院林业研究所 | Method for improving seedling survival rate of hybrid filial generation of populus nigra |
| CN112868529B (en) * | 2021-02-04 | 2022-07-05 | 中国林业科学研究院林业研究所 | Method for improving seedling survival rate of hybrid filial generation of populus nigra |
| CN113575151A (en) * | 2021-07-28 | 2021-11-02 | 东北林业大学 | Poplar cuttage stable rooting method |
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