CN102550382B - Rooting transplanting method for tissue culture poplar seedlings - Google Patents
Rooting transplanting method for tissue culture poplar seedlings Download PDFInfo
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Abstract
A rooting transplanting method for tissue culture poplar seedlings is used for solving the problem that the existing rooting transplanting methods lead to low survival rate of the tissue culture poplar seedlings. The rooting transplanting method for tissue culture poplar seedlings includes: firstly, placing tissue culture poplar seedlings into test tubes containing liquid rooting medium, erecting the tissue culture poplar seedlings, covering openings of the test tubes with seal films, allowing the seedlings to root to obtain the rooted seedlings; secondly, moving the rooted seedlings with 12-14mm long roots to a container with distilled water from the test tubes, and covering the rooted seedlings with a transparent container to preserve moisture for water culture; and thirdly, transplanting the rooted seedlings subjected to water culture to soil, covering the rooted seedlings with a transparent container to preserve moisture for 16 days, removing the transparent container, transplanting the rooted seedlings with the soil into a greenhouse and completing rooting and transplanting of the tissue culture poplar seedlings. The rooting transplanting method is safe and reliable, and the transplant survival rate of the tissue culture poplar seedlings transplanted into the greenhouse can reach more than 95%.
Description
Technical field
The present invention relates to a kind of rooting and transplant method of willow group training seedling.
Background technology
Plant Tissue Breeding comprises the explant collection, a series of processes such as the preparation of medium, can, sterilization, deposit, inoculation, cultivation.Need to carry out rooting and transplant after acquisition group training seedling, conventional method uses solid culture medium to take root, and indoor hardening or soil hardening are directly transplanted the rooting and transplant method of booth afterwards.Willow group training seedling is directly transplanted the easy dehydration of booth hardening and is wilted through after culture of rootage, causes organizing the survival rate of training seedling low.After willow group training seedling process solid culture of rootage, clean the process of root medium and easily hinder root, reduced the water absorbing capacity of root.Conventional method need to be carried out spraying and moisturizing to growing environment, and is higher to environmental requirement, and blade is very easy to dehydration and wilts, and causes organizing the transplanting survival rate of training seedling low.
Summary of the invention
The present invention will solve the low problem of survival rate that existing rooting and transplant method makes willow group training seedling, and a kind of rooting and transplant method of willow group training seedling is provided.
The rooting and transplant method of willow group training seedling of the present invention, carry out according to the following steps: one, willow group training seedling is put into the test tube that 4 ~ 6mL liquid root media is housed, make group training seedling upright, on the test tube lid, envelope bottle film, took root 11 ~ 13 days, the seedling of must taking root; Two, the seedling of taking root that root is reached 12 ~ 14mm moves on to from test tube in the container that distilled water is housed, and the depth of water is 10 ~ 20mm, buckles the transparent vessel moisturizing on the seedling of taking root, water planting 2 days; Three, then will take root transplantation of seedlings in soil after water planting, buckled the transparent vessel moisturizing 16 days on the seedling of taking root, wherein water 1 time front 4 day every day, water rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in afterwards 6 days every 2 days, and watered rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in last 6 days every 3 days, and then removed transparent vessel, the seedling balled transplanting of taking root in soil to booth, is namely completed the rooting and transplant of willow group training seedling; Wherein the described soil of step 3 is comprised of by weight 6:1 turfy soil and vermiculite.
Advantage of the present invention is as follows:
1, the inventive method is safe and reliable, be transplanted to booth after transplanting survival rate reach more than 95%.
2, utilize Boiling tube to take root, traditional existing rooting method will add agar in medium, like this, just must wash away the residual agar of root before transplanting, and easily hinders root, and transplanting survival rate is reduced.The present invention is taken root with Boiling tube, and willow group training seedling can be upright in test tube, takes root with the liquid root media, removed the step of washing agar from before transplanting, and avoided hindering root, improved transplanting survival rate.
3, due to the sucrose that contains 2% in root media, the osmotic pressure of medium is higher like this, is transplanted to soil, and afterwards, osmotic pressure acutely changes, and affects transplanting survival rate.Cultivated in water 2 days after the present invention is taken root, change the osmotic pressure environment of root, itself and the soil of transplanting are approached, make the root after transplanting recover rapidly water absorbing capacity in soil, increase transplanting survival rate.
4, the present invention detains the transparent vessel moisturizing on the seedling of taking root, and not limited by indoor humidity, and has reduced the evaporation of moisture, has avoided the poplar leaf dehydration, has increased the survival rate of plant.
5, soil need not be sterilized: soil sterilization is the material circumstance of traditional group training transplantation of seedlings, transplant on a large scale, the soil sterilization difficulty, traditional rooting method is put into sucrose and agar in medium, wash away in the process of agar, has agar residual, also have sucrose residual, increased like this possibility of root contaminating microorganisms, and then produced rotten, survival rate reduces.The inventive method carries out that liquid is taken root and the program of water planting, has reduced the residual of root sucrose and agar, and then has reduced the possibility of contaminating microorganisms, therefore, utilizes this method to take root, and transplants soil and need not sterilize, and is convenient and practical.
Description of drawings
Fig. 1 is the willow group training seedling photo that liquid is taken root in test tube in embodiment seven; Fig. 2 is the seedling photo of taking root that obtained after taking root in embodiment seven 12 days; Fig. 3 is that the willow that rooting method traditional in embodiment seven was taken root 12 days is organized the photo of training seedling; Fig. 4 is the seedling photo of taking root of water planting in step 2 in embodiment seven; Fig. 5 is the photo of transplantation of seedlings in the soil of taking root in step 3 in embodiment seven; Fig. 6 be in embodiment seven in rapid three moisturizing remove the seedling photo of taking root of transparent plastic cup after 16 days.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the rooting and transplant method of present embodiment willow group training seedling, carry out according to the following steps:: one, willow group training seedling is put into the test tube that 4 ~ 6mL liquid root media is housed, make group training seedling upright, envelope bottle film on the test tube lid, took root 11 ~ 13 days, the seedling of must taking root; Two, the seedling of taking root that root is reached 12 ~ 14mm moves on to from test tube in the container that distilled water is housed, and the depth of water is 10 ~ 20mm, buckles the transparent vessel moisturizing on the seedling of taking root, water planting 2 days; Three, then will take root transplantation of seedlings in soil after water planting, buckled the transparent vessel moisturizing 16 days on the seedling of taking root, wherein water 1 time front 4 day every day, water rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in afterwards 6 days every 2 days, and watered rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in last 6 days every 3 days, and then removed transparent vessel, the seedling balled transplanting of taking root in soil to booth, is namely completed the rooting and transplant of willow group training seedling; Wherein the described soil of step 3 is comprised of by weight 6:1 turfy soil and vermiculite.
In the rooting and transplant process of present embodiment, temperature remains on 25 ℃, and illumination condition is 8 hours dark of illumination 16 hours every days.
Embodiment two: what present embodiment was different from embodiment one is: the group of willow described in step 1 training seedling is that 6 ~ 9cm and stem are slightly 0.6 ~ 1.5mm with a terminal bud, 6 ~ 8 lateral lobes, plant heights.Other is identical with embodiment one.
Embodiment three: what present embodiment was different from embodiment one or two is: the diameter of test tube described in step 1 is 30mm, and length is 20cm.Other is identical with embodiment one or two.
Embodiment four: what present embodiment was different from one of embodiment one to three is: in step 1, willow group training seedling is put into the test tube that 5mL liquid root media is housed.Other is identical with one of embodiment one to three.
Embodiment five: what present embodiment was different from one of embodiment one to four is: took root in step 1 12 days.Other is identical with one of embodiment one to four.
Embodiment six: what present embodiment was different from one of embodiment one to five is: in step 2, the depth of water is 15mm.Other is identical with one of embodiment one to five.
Embodiment seven: the rooting and transplant method of present embodiment willow group training seedling, carry out according to the following steps: will be one, that 6 ~ 9cm and stem are slightly that the mountain new poplar group training seedling of 0.6 ~ 1.5mm is put into the test tube that 5mL liquid root media is housed with a terminal bud, 6 ~ 8 lateral lobes, plant heights, make group training seedling upright, envelope bottle film on the test tube lid, took root 12 days, the seedling of must taking root; Two, the seedling of taking root that root is reached 12 ~ 14mm moves on to from test tube in the plastic cup that distilled water is housed, and the depth of water is 15mm, buckles the moisturizing of transparent plastic cup on the seedling of taking root, water planting 2 days; Three, then will take root transplantation of seedlings in soil after water planting, buckled the transparent vessel moisturizing 16 days on the seedling of taking root, wherein water 1 time front 4 day every day, water rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in afterwards 6 days every 2 days, and watered rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in last 6 days every 3 days, and then removed transparent vessel, the seedling balled transplanting of taking root in soil to booth, is namely completed the rooting and transplant of willow group training seedling; Wherein the described soil of step 3 is comprised of by weight 6:1 turfy soil and vermiculite.
In the rooting and transplant process of present embodiment, temperature remains on 25 ℃, and illumination condition is 8 hours dark of illumination 16 hours every days.
The diameter of test tube described in step 1 is 30mm, and length is 20cm, sows rich garden supplies Co., Ltd by Shanghai and produces.
The root media of liquid described in step 1 is for containing 0.25mg/L NAA(α-naphthaleneacetic acid) and the MS medium of 20g/L sucrose.
The new poplar in mountain described in step 1 is take aspen (Populus.dividiana) as maternal, the artificial hybridization seeds take Xinjiang poplar (Populus.bolleana) as male parent.
The transplanting survival rate of present embodiment mountain new poplar group training seedling is 98%..
The present embodiment mountain new poplar group training seedling photo that liquid is taken root in test tube as shown in Figure 1, what obtained after taking root 12 days takes root the seedling photo as shown in Figure 2, the photo of the willow group training seedling that traditional rooting method was taken root 12 days as shown in Figure 3, can find out on the root of conventional method and adhere to medium, just easily hinder root after cleaning.In step 2 water planting take root the seedling photo as shown in Figure 4, take root the photo of transplantation of seedlings in the soil in step 3 as shown in Figure 5, what in step 3, the transparent plastic cup was removed in moisturizing after 16 days takes root the seedling photo as shown in Figure 6.
Embodiment eight: the rooting and transplant method of present embodiment willow group training seedling, carry out according to the following steps: will be one, that 6 ~ 9cm and stem are slightly that the group training seedling of the European-American Poplar 108 of 0.6 ~ 1.5mm is put into the test tube that 5mL liquid root media is housed with a terminal bud, 6 ~ 8 lateral lobes, plant heights, make group training seedling upright, envelope bottle film on the test tube lid, took root 12 days, the seedling of must taking root; Two, the seedling of taking root that root is reached 12 ~ 14mm moves on to from test tube in the plastic cup that distilled water is housed, and the depth of water is 15mm, buckles the moisturizing of transparent plastic cup on the seedling of taking root, water planting 2 days; Three, then will take root transplantation of seedlings in soil after water planting, buckled the transparent vessel moisturizing 16 days on the seedling of taking root, wherein water 1 time front 4 day every day, water rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in afterwards 6 days every 2 days, and watered rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in last 6 days every 3 days, and then removed transparent vessel, the seedling balled transplanting of taking root in soil to booth, is namely completed the rooting and transplant of willow group training seedling; Wherein the described soil of step 3 is comprised of by weight 6:1 turfy soil and vermiculite.
In the rooting and transplant process of present embodiment, temperature remains on 25 ℃, and illumination condition is 8 hours dark of illumination 16 hours every days.
The diameter of test tube described in step 1 is 30mm, and length is 20cm, sows rich garden supplies Co., Ltd by Shanghai and produces.
The root media of liquid described in step 1 is for containing 0.4mg/L IBA(indolebutyric acid) and the WPM medium of 20g/L sucrose.
European-American Poplar described in step 1 108 is the crossbreed of eastern cottonwood (Populus deltoids) and Populus nigra (Populus nigra).
The transplanting survival rate of the group training seedling of present embodiment European-American Poplar 108 is 97%.
Claims (6)
1. a willow is organized the rooting and transplant method of training seedling, the rooting and transplant method that it is characterized in that willow group training seedling, carry out according to the following steps: one, willow group training seedling is put into the test tube that 4～6mL liquid root media is housed, make group training seedling upright, envelope bottle film on the test tube lid, took root 11～13 days, the seedling of must taking root; Two, the seedling of taking root that root is reached 12～14mm moves on to from test tube in the container that distilled water is housed, and the depth of water is 10～20mm, buckles the transparent vessel moisturizing on the seedling of taking root, water planting 2 days; Three, then will take root transplantation of seedlings in soil after water planting, buckled the transparent vessel moisturizing 16 days on the seedling of taking root, wherein water 1 time front 4 day every day, water rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in afterwards 6 days every 2 days, and watered rear use running water in the spraying of transparent vessel inwall once at every turn; Watered 1 time in last 6 days every 3 days, and then removed transparent vessel, the seedling balled transplanting of taking root in soil to booth, is namely completed the rooting and transplant of willow group training seedling; Wherein the described soil of step 3 is comprised of by weight 6: 1 turfy soil and vermiculite.
2. the rooting and transplant method of a kind of willow group training seedling according to claim 1, is characterized in that the group of willow described in step 1 training seedling is that 6～9cm and stem are slightly 0.6～1.5mm with a terminal bud, 6～8 lateral lobes, plant heights.
3. a kind of willow group according to claim 1 and 2 is trained the rooting and transplant method of seedling, and the diameter that it is characterized in that test tube described in step 1 is 30mm, and length is 20cm.
4. the rooting and transplant method of a kind of willow group training seedling according to claim 3, is characterized in that in step 1, willow group training seedling being put into the test tube that 5mL liquid root media is housed.
5. the rooting and transplant method of a kind of willow group training seedling according to claim 4, is characterized in that taking root in step 1 12 days.
6. the rooting and transplant method of a kind of willow group training seedling according to claim 5, is characterized in that in step 2, the depth of water is 15mm.
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| CN104335887A (en) * | 2014-10-14 | 2015-02-11 | 金陵科技学院 | Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid |
| CN105052715B (en) * | 2015-08-03 | 2017-07-25 | 山东省林业科学研究院 | A kind of Poplar Breeding device and its application method |
| CN108575359A (en) * | 2018-04-27 | 2018-09-28 | 北京农学院 | A kind of processing of Malus admiring fruit tree training tissue culture seedling and tissue-cultured seedling rooting method |
| CN108849509A (en) * | 2018-07-05 | 2018-11-23 | 内蒙古和盛生态育林有限公司 | A kind of cultural method of poplar microbiological contamination tissue-cultured seedling |
| CN112868529B (en) * | 2021-02-04 | 2022-07-05 | 中国林业科学研究院林业研究所 | Method for improving seedling survival rate of hybrid filial generation of populus nigra |
| CN113575151B (en) * | 2021-07-28 | 2022-11-01 | 东北林业大学 | Poplar cuttage stable rooting method |
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